Resistance identification method of sweet potato leaf curl virus disease

Abstract

The invention discloses a resistance identification method of sweet potato leaf curl virus disease. The method includes following steps: obtaining original seedlings not containing leaf curl virus disease SPLCV after a to-be-identified variety is subjected to culture of virus-free test tube seedlings and molecular detection; putting the original seedlings into hole trays for quick propagation; allowing high-power expanding propagation of leaf curl virus indicating plants; identifying insect-borne virus inoculation when variety virus-free tissue culture seedlings are isolated and in an airtightcondition; identifying grafting virus inoculation in a plant net room condition; performing yield comparison experiment planting, harvesting, measuring yield, and conserving seeds; discharging seedsin the next year, and investigating and identifying emerging seedling leaf curl virus symptom showing rate; evaluating leaf curl virus resistant (tolerant) characteristics of the variety. Propagationlaws of the SPLCV are utilized, natural virus transmission, insect-borne virus inoculation and grafting virus inoculation are performed on the identified variety at different stages, grading is performed according to grading standards through seedling bed performance of each variety in the next year, resistant, tolerant and sensitive characteristics of the variety to the SPLCV are determined, anda basic resistant source is provided for breeding of SPLCV resistant varieties.

Description

Technical field [0001] The invention relates to a method for identifying resistance to sweet potato leaf roll virus disease. Background technique [0002] As the seventh largest food crop in the world, sweet potato is mainly distributed in Asia, Africa, Latin America and other regions. China's planting area accounts for about 80% of the world's total. However, due to the widespread existence of sweet potato virus disease, the yield and quality of sweet potato were seriously affected. According to data, the incidence rate of field varieties of sweet potato virus disease is 100%. In East Africa and South America, the sweet potato production has been greatly reduced or even ceased due to the virus disease. The United States and Japan have also successively reported that sweet potato virus disease has reduced the yield and quality of sweet potato. Disease is an important disease in China's sweetpotato producing areas, and it is an important reason for the degradation of sweetpotatoe...

AI Technical Summary

Problems solved by technology

The above three methods have certain feasibility for the evaluation of virus resistance of some viruses in sweet potato varieties, but there are many problems in the identification of sweet potato leafroll virus, which mainly uses insects as mediators, because there are two types of virus resistance performance of varieties: one is variety It has anti-virus genes, and the other is to reduce insect-borne viruses and avoid diseases

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