50 results about "Indicator plant" patented technology

The invention provides a Pseudomonas.chlororaphis subsp.Aurantiaca Pa40, preservation number of which is CGMCCNo.2764. The strain generates HCN, prolease and siderophore relative to antibacterium and has plate board inhibition effect on a majority of tested plant pathogenic fungi and a part of tested plant pathogenic bacteria. Control effect of the biocontrol strain Pa40 on rhizoctonia solani is detected by taking wheat as an indicator plant on the plate, in the greenhouses and in the fields; a result shows that the control effects of the Pa40 on the rhizoctonia solani on the plate bed, in the greenhouse and in the fields are respectively 53.97%, 68.10% and 72.46%; wherein the control effects on the rhizoctonia solani in the greenhouses and fields are higher than that of validamycin.

Methods, devices, and systems may be utilized for detecting one or more properties of a plant area and generating a map of the plant area indicating at least one property of the plant area. The system comprises an inspection system associated with a transport device, the inspection system including one or more sensors configured to generate data for a plant area including to: capture at least 3D image data and 2D image data; and generate geolocational data. The datacenter is configured to: receive the 3D image data, 2D image data, and geolocational data from the inspection system; correlate the 3D image data, 2D image data, and geolocational data; and analyze the data for the plant area. A dashboard is configured to display a map with icons corresponding to the proper geolocation and image data with the analysis.

The invention relates to a stem tip detoxification and cultivation method for a sweet potato virus seedling. The method comprises the following steps: (1) taking a sweet potato block infected by sweet potato feathery mottle virus and/or sweet potato G virus; cultivating the sweet potato block indoors to sprout; (2) shearing scissoring terminal buds growing to 1.5-2.0 cm for disinfection; (3) peeling off the stem tips with two leaf primordiums in a sterile manner among the terminal buds as explants for vaccination cultivation to obtain regeneration plants; (4) conducting 2-3 times of successive transfer cultivation on the regeneration plants obtained in the step (3) to build a strain; conducting inspection by adopting an indicator plant method and a cellulose nitrate membrane ELISA in sequence to obtain a detoxified strain; (5) proliferating the detoxified strain quickly to obtain the detoxification seedling. The method can quickly build cultivation system suitable for stem tip cultivation and rapid propagation detoxification seedlings of majority of sweet potato varieties/strains.

The invention discloses a detection method for residual imazapic in soil. The detection method comprises the following steps: acquiring soil of a to-be-detected peanut field before crop rotation and potting an indicative plant sensitive to imazapic in the soil of the to-be-detected peanut field; potting another indicative plant sensitive to imazapic in control soil, i.e., a seedling growing matrix frequently used in gardening; and observing and comparing the growth conditions of the seedling of the sensitive plant potted in the soil of the to-be-detected peanut field and the seedling of the sensitive plant potted in the control soil and determining whether residual imazapic in the soil of the peanut field is safe to later rape and wheat to be planted in the field. Compared with conventional instrumental analysis and determination methods, the detection method provided by the invention has the characteristics of easiness, low cost and good operability; and the method can be easily learned and used, does not need professionals and expensive equipment and reagents, is easy to promote and apply and provides effective bases for field safety production and management.

The invention discloses a resistance identification method of sweet potato leaf curl virus disease. The method includes following steps: obtaining original seedlings not containing leaf curl virus disease SPLCV after a to-be-identified variety is subjected to culture of virus-free test tube seedlings and molecular detection; putting the original seedlings into hole trays for quick propagation; allowing high-power expanding propagation of leaf curl virus indicating plants; identifying insect-borne virus inoculation when variety virus-free tissue culture seedlings are isolated and in an airtightcondition; identifying grafting virus inoculation in a plant net room condition; performing yield comparison experiment planting, harvesting, measuring yield, and conserving seeds; discharging seedsin the next year, and investigating and identifying emerging seedling leaf curl virus symptom showing rate; evaluating leaf curl virus resistant (tolerant) characteristics of the variety. Propagationlaws of the SPLCV are utilized, natural virus transmission, insect-borne virus inoculation and grafting virus inoculation are performed on the identified variety at different stages, grading is performed according to grading standards through seedling bed performance of each variety in the next year, resistant, tolerant and sensitive characteristics of the variety to the SPLCV are determined, anda basic resistant source is provided for breeding of SPLCV resistant varieties.

The invention discloses protein for plant response to low-phosphorus stress and high-salt stress, a coding gene and application thereof. The protein is protein which has the following amino acid sequences: 1) the protein which has the amino acid residue sequence of SEQ ID No.2 in a sequence table; and 2) the protein which causes the amino acid residue sequence of the SEQ ID No.2 in the sequence table to be replaced and/or lose and/or add by one or more amino acid residues, has functions of improving the plant sensitivity to low-phosphorus stress and high-salt stress and is derived from the SEQ ID No.2. The gene can be used for cultivating new plant varieties with low-phosphorus resistance and high-salt resistance, as well as indicator plants which are sensitive to low phosphorus and high salt.

The invention discloses a method of detecting hidden ground objects under a vegetation background based on an unmanned gyroplane. The method comprises steps of scanning a target area row by row by theunmanned gyroplane carrying a hyperspectral imaging spectrometer, and acquiring spectrum data and storing the data; calculating fluorescence value information of the target area by using a fluorescence extraction algorithm; acquiring a remote sensing high resolution optical remote sensing image of the target area through a satellite, and preprocessing the fluorescence value information and the remote sensing image so as to obtain fluorescence value information; and finally, extracting a fluorescent probe from the fluorescence value information by using Fraunhofer dip method so as to determinewhether the target area has hidden ground objects. The detection method can better detect vegetation camouflage technologies, and can be widely used in the national defense area. The detected chlorophyll fluorescence can be used for indicating physiological and biochemical processes of plant cells, reflects real working states of plant photosynthesis timely and accurately and diagnoses health state of plants.

The invention discloses a preparation method of functional chromogenic health indication plant deodorization cat litter. The functional chromogenic health indication plant deodorization cat litter has a health indication function, further has antibacterial and deodorization effects and is good in water absorption and clustering property. According to technical key point, the functional chromogenic health indication plant deodorization cat litter is prepared from the following components in parts by weight: 40%-60% of modified bean dreg powder, 15%-30% of modified plant fiber, 10%-30% of a filler, 1%-10% of a plant adhesive, 10%-20% of a compound indicator and 1%-10% of a functional additive. According to the preparation method of the functional chromogenic health indication plant deodorization cat litter, the modified bean dreg powder and the modified plant fiber are prepared by adopting a specific technology, so that the water absorption and the agglomeration property are better; a compound indicator can realize an acid-base chromogenic reaction on cat urine, so that the health condition of pets is warned; and in addition, functional plant additives are added, so that the functional chromogenic health indication plant deodorization cat litter has the effects of inhibiting bacteria and deodorizing.

The present invention is a kind of method for in vitro culture of sweet potato stem tip and detection of virus in tissue culture seedlings. The specific steps are: (1) material collection and disinfection: in June, cut the top buds of sweet potato seedlings growing vigorously in the field, cut off the leaves, Rinse with tap water, dry the water with sterile filter paper, and place it on an ultra-clean workbench for disinfection; (2) Stripping and culturing of shoot tips: Under a binocular dissecting microscope, peel off the outer surface of the sterilized sweet potato shoots with the tip of a syringe. (3) virus detection of tissue culture seedlings: virus detection was carried out by using the needle-pricking juice dipping method of indicator plants, and the virus-carrying seedlings were eliminated according to the detection results. Keep the virus-free seedlings. The invention provides a method for in vitro culture of sweet potato shoot tips and virus detection of tissue cultured seedlings. The success rate of sweet potato detoxification is improved, the accuracy of virus detection is high, and an effective detection means is provided for preventing and controlling sweet potato virus diseases.

The invention discloses a rice sugar-induced promoter SRN1 and application thereof. Belongs to the technical field of gene engineering. The nucleotide sequence of the rice sugar-induced promoter SRN1 is as shown in SEQ ID No. 1. The promoter SRN1 belongs to a tissue-specific promoter induced by cane sugar and glucose, and has important application value in the field of plant genetic engineering and research on the conduction mechanism of sugar signal molecules in organisms. The promoter SRN1 provides a tool for directional expression of genes for genetic engineering breeding of important agronomic traits and adversity stress coping of rice or other food crops, and can also be used for indicating the level of soluble sugar in plants in scientific research.

The current invention provides a method for rapid testing of stress tolerance in a plant and a method of producing plants with enhanced stress tolerance, in particular cold and/or drought tolerance. Such a method may be applied in breeding and selection programs for this trait, or for determining the timing of induction of stress and cold tolerance or hardening of a plant, for instance for agricultural purposes. The current invention exploits a difference in the temperature and light regimen induced transcriptional regulation of several types of dehydrins, in order to determine a ratio of dehydrin types that is indicative of cold and/or drought tolerance, induction of hardening and dormancy in a plant.

The invention provides a kit and a detection method for indicating subcellular localization of plant nucleolus and a cajal body. The kit comprises two fusion protein tobacco fiber proteins, which are fusion protein YFP-NbFib2 with yellow fluorescence and fusion protein CFP-NbFib2 with cyan fluorescence. The specificity of the kit adopted in the invention is higher, and the nucleolus and cajal body subcellular organelle on the cell nucleus can be specifically indicated; the operation is simpler; pre-observed protein can be injected simultaneously, so that the damage for plants is reduced; the operation is safer; cancerogenic substances such as poisonous substances are prevented from contacting in the process of using DAPI; the result judgment is simple; and the color of the fluorescence is observed just in a confocal state.

A farming machine includes one or more image sensors for capturing an image as the farming machine moves through the field. A control system accesses an image captured by the one or more sensors and classifies each pixel in the image as ground pixels or plant pixels. The control system then determines a ground plane for the image based on the classified ground pixels and corrects the pixels in the image based on the determined ground plane. Plant pixels in the image can represent plants and the control system identifies plant pixel clusters as plants. The difference in height of plants relative to other plants in a field can be indicative for plant treatments. As such, the control system determines a height of each plant pixel cluster relative to the ground plane and compares the height of the pixel cluster to other plant pixel clusters in the field. Based on the determined height dissimilarity, the control system can identify the plant for treatment and actuate treatment mechanisms to treat the plant.

The invention relates to a nondestructive screening method for plant genetic transformation and application thereof. Plant genetic transformation depends on an efficient screening system, and a commonscreening system comprises antibiotic screening and reporting system screening. The existing reporting system screening needs special equipment and expensive chemicals or destructive treatment on biological samples. The invention constructs a new reporter gene RUBY, and the enzyme synthesized by the gene can convert tyrosine into red betacyanin. The invention utilizes RUBY to indicate the occurrence of plant genetic transformation, and successfully realizes nondestructive screening of naked eye visible plant genetic transformation under visible light.

The invention discloses a cultivation method of moss for biological monitoring, which comprises the following steps: collecting a whole piece of moss, cutting off the rhizoid of the moss, sieving the collected whole piece of moss for the first time, and sowing for the first time; cutting off rhizoid with moss after first-time sowing, performing second-time sieving, and preparing for second-time sowing; a moss culture solution is prepared, the moss culture solution is used for conducting second-time sowing and cultivation on moss, and protonemata is obtained; preparing a moss attachment matrix and an attachment matrix skeleton; the protonemata obtained in the second step is sown and attached to a moss attachment substrate, the protonemata differentiates on the moss attachment substrate to generate gametophytes, and the gametophytes grow on the moss attachment substrate to form target moss. The moss for biological monitoring cultivated by the invention has high sensitivity to micro-plastics in the environment, can effectively monitor the micro-plastics in the environment, and can be used as a preferred environmental micro-plastic pollution indication plant.

The invention discloses a research method for a succession process mechanism of a three-river-source alpine marsh wetland, and belongs to the technical field of environmental engineering, and the method comprises the following steps: selecting four indicating plants: Kobresia tibetica, Kobresia pygmaea, Carex scabrirostris and Carex muliensis. according to the method, hilly depression microtopography degradation of the alpine marsh wetland is a progressive process of transition from a primary wetland to a dry wetland and finally transition to an alpine meadow. The research result of the invention is beneficial to comprehensive understanding of the degradation process of the three-river-source alpine marsh wetland ecosystem.

The invention provides a transgenic soybean indicating auxin concentrations. The soybean repeats construction of DR5V2 promoters many times by using short promoter regions of auxin responding key genes, and beta-glucosidase (GUS) reporter genes are connected. The transgenic soybean can respond to the change of the auxin concentrations. After a GUS substrate, namely, 5-bromine-4-chlorine-3-indole-beta-gluconate(X-gluc), is stained, the distribution areas and concentration change of the auxin can be observed by a microscopy. Compared with a previously published soybean-auxin indicator plant pGH3-GUS, the provided DR5-V2-GUS transgenic soybean is more accurate in auxin indicating.