52 results about "Indicator plant" patented technology

The invention provides a Pseudomonas.chlororaphis subsp.Aurantiaca Pa40, preservation number of which is CGMCCNo.2764. The strain generates HCN, prolease and siderophore relative to antibacterium and has plate board inhibition effect on a majority of tested plant pathogenic fungi and a part of tested plant pathogenic bacteria. Control effect of the biocontrol strain Pa40 on rhizoctonia solani is detected by taking wheat as an indicator plant on the plate, in the greenhouses and in the fields; a result shows that the control effects of the Pa40 on the rhizoctonia solani on the plate bed, in the greenhouse and in the fields are respectively 53.97%, 68.10% and 72.46%; wherein the control effects on the rhizoctonia solani in the greenhouses and fields are higher than that of validamycin.

Methods, devices, and systems may be utilized for detecting one or more properties of a plant area and generating a map of the plant area indicating at least one property of the plant area. The system comprises an inspection system associated with a transport device, the inspection system including one or more sensors configured to generate data for a plant area including to: capture at least 3D image data and 2D image data; and generate geolocational data. The datacenter is configured to: receive the 3D image data, 2D image data, and geolocational data from the inspection system; correlate the 3D image data, 2D image data, and geolocational data; and analyze the data for the plant area. A dashboard is configured to display a map with icons corresponding to the proper geolocation and image data with the analysis.

By providing a control system in communication with a hydraulic system for adjusting an angle of smoothing tools of a harrow, and a communications system for determining a present geographic location of the tillage implement in communication with the control system, the angle of the smoothing tools can be adjusted according to presently determined coordinate locations on a map which indicates amounts of plant residue. This may allow an automated process in which smoothing tools can be continuously adjusted during a tilling session in order to provide the most aggressive setting for optimum field leveling and residue flow based on field conditions.

The invention relates to a rapid virus detection method of monorchid herminium herb virus-free seedlings of tissue culturing, which belongs to the technology field of plant virus detection, including the following steps: preparing the culture medium, stem tip virus-free culturing, virus-free seedling culturing of the indicator plant tomato, micrografting culturing and the proliferation culture of the virus-free seedlings of tissue culturing. The method has the advantages that the adopted culture medium, tissue culturing conditions and the micrograft cutter are applicable to the micrografting culturing of the virus-free seedlings and facilitating the healing of the graft unions with a grafting rate at more than 90%, which fully meet the requirement of the virus detection. Compared with the conventional field indicator plant detection, the method greatly reduces the required detection time, which is free from time and environmental factors and can be carried out under the laboratory conditions throughout the year. The method is very practical and applicable to the rapid virus detection of the monorchid herminium herb virus-free seedlings and the mass production of the virus-free seedlings.

The invention relates to a stem tip detoxification and cultivation method for a sweet potato virus seedling. The method comprises the following steps: (1) taking a sweet potato block infected by sweet potato feathery mottle virus and / or sweet potato G virus; cultivating the sweet potato block indoors to sprout; (2) shearing scissoring terminal buds growing to 1.5-2.0 cm for disinfection; (3) peeling off the stem tips with two leaf primordiums in a sterile manner among the terminal buds as explants for vaccination cultivation to obtain regeneration plants; (4) conducting 2-3 times of successive transfer cultivation on the regeneration plants obtained in the step (3) to build a strain; conducting inspection by adopting an indicator plant method and a cellulose nitrate membrane ELISA in sequence to obtain a detoxified strain; (5) proliferating the detoxified strain quickly to obtain the detoxification seedling. The method can quickly build cultivation system suitable for stem tip cultivation and rapid propagation detoxification seedlings of majority of sweet potato varieties / strains.

The invention discloses a method for determining water demand of a detected plant by utilizing water demand information of an indicator plant. The method comprises the following steps: first, planting an indicator plant and a detected plant at a same time in the same environment by a same cultivation method; respectively measuring a CO2 response curve of leaf photosynthesis of the detected plant and the indicator plant by a portable photosynthesis apparatus through a conventional method; second, on the basis of net photosynthetic rate and transpiration rate of the detected plant and the indicator plant under different carbon dioxide concentrations in the CO2 response curves measured by the portable photosynthesis apparatus, calculating water utilization rate of the detected plant and the indicator plant under different carbon dioxide concentrations; third, fitting the water utilization rate data of the detected plant with the water utilization rate data of the indicator plant under a corresponding carbon dioxide concentration into a proportional function; and acquiring an oblique ratio of the above equation, so as to obtain a water utilization coefficient of the detected plant; fourth, respectively determining net photosynthetic rate of the detected plant leaf and the indicator plant leaf by using the portable photosynthesis apparatus through a conventional method; and fifth, according to the water utilization coefficient of the detected plant, the net photosynthetic rate of the detected plant leaf and the indicator plant leaf, and the water demand of the indicator plant, acquiring the water demand of the detected plant.

The invention discloses a detection method for residual imazapic in soil. The detection method comprises the following steps: acquiring soil of a to-be-detected peanut field before crop rotation and potting an indicative plant sensitive to imazapic in the soil of the to-be-detected peanut field; potting another indicative plant sensitive to imazapic in control soil, i.e., a seedling growing matrix frequently used in gardening; and observing and comparing the growth conditions of the seedling of the sensitive plant potted in the soil of the to-be-detected peanut field and the seedling of the sensitive plant potted in the control soil and determining whether residual imazapic in the soil of the peanut field is safe to later rape and wheat to be planted in the field. Compared with conventional instrumental analysis and determination methods, the detection method provided by the invention has the characteristics of easiness, low cost and good operability; and the method can be easily learned and used, does not need professionals and expensive equipment and reagents, is easy to promote and apply and provides effective bases for field safety production and management.

Methods, devices, and systems may be utilized for detecting one or more properties of a plant area and generating a map of the plant area indicating at least one property of the plant area. The system comprises an inspection system associated with a transport device, the inspection system including one or more sensors configured to generate data for a plant area including to: capture at least 3D image data and 2D image data; and generate geolocational data. The datacenter is configured to: receive the 3D image data, 2D image data, and geolocational data from the inspection system; correlate the 3D image data, 2D image data, and geolocational data; and analyze the data for the plant area. A dashboard is configured to display a map with icons corresponding to the proper geolocation and image data with the analysis.

The invention discloses a resistance identification method of sweet potato leaf curl virus disease. The method includes following steps: obtaining original seedlings not containing leaf curl virus disease SPLCV after a to-be-identified variety is subjected to culture of virus-free test tube seedlings and molecular detection; putting the original seedlings into hole trays for quick propagation; allowing high-power expanding propagation of leaf curl virus indicating plants; identifying insect-borne virus inoculation when variety virus-free tissue culture seedlings are isolated and in an airtightcondition; identifying grafting virus inoculation in a plant net room condition; performing yield comparison experiment planting, harvesting, measuring yield, and conserving seeds; discharging seedsin the next year, and investigating and identifying emerging seedling leaf curl virus symptom showing rate; evaluating leaf curl virus resistant (tolerant) characteristics of the variety. Propagationlaws of the SPLCV are utilized, natural virus transmission, insect-borne virus inoculation and grafting virus inoculation are performed on the identified variety at different stages, grading is performed according to grading standards through seedling bed performance of each variety in the next year, resistant, tolerant and sensitive characteristics of the variety to the SPLCV are determined, anda basic resistant source is provided for breeding of SPLCV resistant varieties.

A method and system for monitoring the health of plants in a field. The method and system acquire a thermal image indicative of thermal energy emitted by the plants and process the thermal image to assess variations in the temperatures among the plants.

The invention discloses protein for plant response to low-phosphorus stress and high-salt stress, a coding gene and application thereof. The protein is protein which has the following amino acid sequences: 1) the protein which has the amino acid residue sequence of SEQ ID No.2 in a sequence table; and 2) the protein which causes the amino acid residue sequence of the SEQ ID No.2 in the sequence table to be replaced and / or lose and / or add by one or more amino acid residues, has functions of improving the plant sensitivity to low-phosphorus stress and high-salt stress and is derived from the SEQ ID No.2. The gene can be used for cultivating new plant varieties with low-phosphorus resistance and high-salt resistance, as well as indicator plants which are sensitive to low phosphorus and high salt.

The invention discloses a method for determining the crop rotation safety interval of bensulfuron methyl residual in soil, and belongs to the technical field of pesticide residual determination. The method adopting soil before crop rotation of wheat field and rice field as identification field soil comprises the following steps: potting a bensulfuron methyl sensible indicative plant in the identification field soil; and measuring and comparing the seedling height, the root length and the overground part fresh weight reduction of the sensible indicative plant in the identification field soil and the sensitive indicative plant in contrast oil after the sensible indicative plant grows for 7 d, and determining the crop rotation safety interval of bensulfuron methyl residual in soil to succeeding crops. The method has the advantages of low price, accuracy in determination, strong operability, easiness in promotion and application, and provision of effective basis for field safety production management.

The invention discloses a method of detecting hidden ground objects under a vegetation background based on an unmanned gyroplane. The method comprises steps of scanning a target area row by row by theunmanned gyroplane carrying a hyperspectral imaging spectrometer, and acquiring spectrum data and storing the data; calculating fluorescence value information of the target area by using a fluorescence extraction algorithm; acquiring a remote sensing high resolution optical remote sensing image of the target area through a satellite, and preprocessing the fluorescence value information and the remote sensing image so as to obtain fluorescence value information; and finally, extracting a fluorescent probe from the fluorescence value information by using Fraunhofer dip method so as to determinewhether the target area has hidden ground objects. The detection method can better detect vegetation camouflage technologies, and can be widely used in the national defense area. The detected chlorophyll fluorescence can be used for indicating physiological and biochemical processes of plant cells, reflects real working states of plant photosynthesis timely and accurately and diagnoses health state of plants.

The invention discloses a preparation method of functional chromogenic health indication plant deodorization cat litter. The functional chromogenic health indication plant deodorization cat litter has a health indication function, further has antibacterial and deodorization effects and is good in water absorption and clustering property. According to technical key point, the functional chromogenic health indication plant deodorization cat litter is prepared from the following components in parts by weight: 40%-60% of modified bean dreg powder, 15%-30% of modified plant fiber, 10%-30% of a filler, 1%-10% of a plant adhesive, 10%-20% of a compound indicator and 1%-10% of a functional additive. According to the preparation method of the functional chromogenic health indication plant deodorization cat litter, the modified bean dreg powder and the modified plant fiber are prepared by adopting a specific technology, so that the water absorption and the agglomeration property are better; a compound indicator can realize an acid-base chromogenic reaction on cat urine, so that the health condition of pets is warned; and in addition, functional plant additives are added, so that the functional chromogenic health indication plant deodorization cat litter has the effects of inhibiting bacteria and deodorizing.

The present invention is a kind of method for in vitro culture of sweet potato stem tip and detection of virus in tissue culture seedlings. The specific steps are: (1) material collection and disinfection: in June, cut the top buds of sweet potato seedlings growing vigorously in the field, cut off the leaves, Rinse with tap water, dry the water with sterile filter paper, and place it on an ultra-clean workbench for disinfection; (2) Stripping and culturing of shoot tips: Under a binocular dissecting microscope, peel off the outer surface of the sterilized sweet potato shoots with the tip of a syringe. (3) virus detection of tissue culture seedlings: virus detection was carried out by using the needle-pricking juice dipping method of indicator plants, and the virus-carrying seedlings were eliminated according to the detection results. Keep the virus-free seedlings. The invention provides a method for in vitro culture of sweet potato shoot tips and virus detection of tissue cultured seedlings. The success rate of sweet potato detoxification is improved, the accuracy of virus detection is high, and an effective detection means is provided for preventing and controlling sweet potato virus diseases.

The invention relates to a method for culturing and planting seedlings of color virus-free sweet potatoes. The method is characterized by utilizing the characteristic of low virus content or no virus content in stem tips of the sweet potatoes, cutting meristems of the stem tips of 0.2-0.4mm under an aseptic manipulation, forming test tube seedlings by culturing on a specific culture medium, and further detecting the virus-free seedlings by serology and indicator plant grafting. Meanwhile, the invention further provides the corresponding tissue rapid propagation technical regulations and a factorized production system according to different types, thereby accelerating the propagation of a large number of color style virus-free sweet potato seedlings. The method can propagate the potato species which has high nutritional value and bright color and is applicable to fresh-eating, baking or processing of the preserved sweet potatoes.

The invention discloses a rice sugar-induced promoter SRN1 and application thereof. Belongs to the technical field of gene engineering. The nucleotide sequence of the rice sugar-induced promoter SRN1 is as shown in SEQ ID No. 1. The promoter SRN1 belongs to a tissue-specific promoter induced by cane sugar and glucose, and has important application value in the field of plant genetic engineering and research on the conduction mechanism of sugar signal molecules in organisms. The promoter SRN1 provides a tool for directional expression of genes for genetic engineering breeding of important agronomic traits and adversity stress coping of rice or other food crops, and can also be used for indicating the level of soluble sugar in plants in scientific research.

The invention provides a method and a device for determining a plant care mode. The method comprises the following steps of: obtaining an image of a plant to be cared for; extracting structural features of the plant to be cared for from the image, the structural features comprising at least one of a root, a stem, a leaf, a flower and a fruit; determining a nursing mode corresponding to the plant to be cared for according to the structural characteristics of the plant to be cared for and a recognition model for indicating a relationship between the structural characteristics of the plant and the nursing mode of the plant. Compared with the prior art, the method saves the manual searching process and improves the efficiency of acquiring the plant care mode.

The invention discloses a plant low potassium sensitive correlated protein AtLKR1, its coding gene and application. The protein is the protein of the following 1) or 2): 1) a protein composed of an amino acid sequence as shown in Sequence 2 of a sequence table; 2) a plant low potassium sensitive correlated protein derived from 1) through the replacement and / or deletion and / or addition of one or more amino acid residues in the amino acid residual sequence as shown in Sequence 2 of the sequence table. After the gene AtLKR1 is expressed in a target plant, the plant shows the low potassium sensitivity, and can be treated as an indication plant for soil potassium index for an early warning of low potassium in soil; otherwise, knocking out the AtLKR1 gene provided by the invention or reducing the AtLKR1 gene expression level, the plant shows low potassium resistant property. The gene provided by the invention is of great significance for the cultivation of new low potassium tolerant species and the research of plants' low potassium tolerance.

The current invention provides a method for rapid testing of stress tolerance in a plant and a method of producing plants with enhanced stress tolerance, in particular cold and / or drought tolerance. Such a method may be applied in breeding and selection programs for this trait, or for determining the timing of induction of stress and cold tolerance or hardening of a plant, for instance for agricultural purposes. The current invention exploits a difference in the temperature and light regimen induced transcriptional regulation of several types of dehydrins, in order to determine a ratio of dehydrin types that is indicative of cold and / or drought tolerance, induction of hardening and dormancy in a plant.

The invention provides a kit and a detection method for indicating subcellular localization of plant nucleolus and a cajal body. The kit comprises two fusion protein tobacco fiber proteins, which are fusion protein YFP-NbFib2 with yellow fluorescence and fusion protein CFP-NbFib2 with cyan fluorescence. The specificity of the kit adopted in the invention is higher, and the nucleolus and cajal body subcellular organelle on the cell nucleus can be specifically indicated; the operation is simpler; pre-observed protein can be injected simultaneously, so that the damage for plants is reduced; the operation is safer; cancerogenic substances such as poisonous substances are prevented from contacting in the process of using DAPI; the result judgment is simple; and the color of the fluorescence is observed just in a confocal state.

A farming machine includes one or more image sensors for capturing an image as the farming machine moves through the field. A control system accesses an image captured by the one or more sensors and classifies each pixel in the image as ground pixels or plant pixels. The control system then determines a ground plane for the image based on the classified ground pixels and corrects the pixels in the image based on the determined ground plane. Plant pixels in the image can represent plants and the control system identifies plant pixel clusters as plants. The difference in height of plants relative to other plants in a field can be indicative for plant treatments. As such, the control system determines a height of each plant pixel cluster relative to the ground plane and compares the height of the pixel cluster to other plant pixel clusters in the field. Based on the determined height dissimilarity, the control system can identify the plant for treatment and actuate treatment mechanisms to treat the plant.

The invention relates to a nondestructive screening method for plant genetic transformation and application thereof. Plant genetic transformation depends on an efficient screening system, and a commonscreening system comprises antibiotic screening and reporting system screening. The existing reporting system screening needs special equipment and expensive chemicals or destructive treatment on biological samples. The invention constructs a new reporter gene RUBY, and the enzyme synthesized by the gene can convert tyrosine into red betacyanin. The invention utilizes RUBY to indicate the occurrence of plant genetic transformation, and successfully realizes nondestructive screening of naked eye visible plant genetic transformation under visible light.

The invention belongs to the technical field of pollution monitoring. The invention discloses a system and a method for determining transfer of atmospheric settling heavy metals in sediments. The system for determining the transfer of atmospheric settling heavy metals in the sediments comprises a sample collection module, a deposition column preparation module, a seawater sample treatment module,an air sample treatment module, a soil sample treatment module, a first calculation module, a central control module, a bryophyte sample treatment module, a bryophyte sample analysis module, a secondcalculation module, a heavy metal transfer comparison module, a cloud storage module and a display module. According to the invention, atmospheric flow and disturbance of the external environment canbe isolated by utilizing the sediment seawater isolation method, the sediment is well protected, and a measurement result can accurately reflect a real numerical value of heavy metal from atmosphericsedimentation in the region; and the bryophyte is used as a biological monitoring indication plant, the atmospheric settlement heavy metal pollution condition is monitored by using a moss bag method,and basic data is provided for ecological environment protection.

The invention discloses a cultivation method of moss for biological monitoring, which comprises the following steps: collecting a whole piece of moss, cutting off the rhizoid of the moss, sieving the collected whole piece of moss for the first time, and sowing for the first time; cutting off rhizoid with moss after first-time sowing, performing second-time sieving, and preparing for second-time sowing; a moss culture solution is prepared, the moss culture solution is used for conducting second-time sowing and cultivation on moss, and protonemata is obtained; preparing a moss attachment matrix and an attachment matrix skeleton; the protonemata obtained in the second step is sown and attached to a moss attachment substrate, the protonemata differentiates on the moss attachment substrate to generate gametophytes, and the gametophytes grow on the moss attachment substrate to form target moss. The moss for biological monitoring cultivated by the invention has high sensitivity to micro-plastics in the environment, can effectively monitor the micro-plastics in the environment, and can be used as a preferred environmental micro-plastic pollution indication plant.

The invention discloses protein for plant response to low-phosphorus stress and high-salt stress, a coding gene and application thereof. The protein is protein which has the following amino acid sequences: 1) the protein which has the amino acid residue sequence of SEQ ID No.2 in a sequence table; and 2) the protein which causes the amino acid residue sequence of the SEQ ID No.2 in the sequence table to be replaced and / or lose and / or add by one or more amino acid residues, has functions of improving the plant sensitivity to low-phosphorus stress and high-salt stress and is derived from the SEQ ID No.2. The gene can be used for cultivating new plant varieties with low-phosphorus resistance and high-salt resistance, as well as indicator plants which are sensitive to low phosphorus and high salt.

The invention discloses a method for judging the safety interval of crop rotation for residual bensulfuron in soil, which belongs to the technical field of pesticide residue determination. The soil before crop rotation in wheat fields and rice fields is used as the identification field soil, and the benzosulfuron-methyl residue is used as the identification field soil. The sensitive indicator plants of sulfuron-methyl are potted into the identification field soil; after the sensitive indicator plants grow for 7 days, the plant height, root length and above-ground parts of the seedlings of the sensitive indicator plants in the identification field soil and the sensitive indicator plants in the control soil are measured and compared. The fresh weight inhibition rate can be used to determine the safety interval of bensulfuron-methyl residues in the field soil for subsequent crop rotations. The invention has the advantages of low price, accurate judgment, strong operability, etc., is easy to popularize and apply, and provides an effective basis for safe production management in the field.