Virus fast detecting method for ginseng fruit tissue cultural seedlings of free-virus

A detection method, ginseng fruit technology, applied in gardening methods, botanical equipment and methods, cultivation, etc., can solve the problems that the symptoms of viral diseases are easily affected by environmental factors and take a long time, so as to achieve the effect of healing and shortening the time

Inactive Publication Date: 2008-08-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method takes a long time and takes 1 year to complete, and the symptoms of viral diseases are easily affected by environmental factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] 1), the preparation of the culture medium, including each component of the basic culture medium and the culture medium of each stage of tissue culture and the weight per liter are:

[0057] (1) Basic medium: choose MS medium or 1 / 2MS medium, agar 8g / L, pH5.8;

[0058] (2) Induction medium: MS+BA 1mg / L+IAA 0.1mg / L+sucrose 30g / L;

[0059] (3) Micro cutting medium: MS+BA0.5mg / L+NAA0.1mg / L+sugar 30g / L;

[0060] (4) Strong seedling medium: MS+BA 0.1mg / L+NAA 0.05mg / L+PP 333 0.2mg / L+white sugar 30g / L;

[0061] (5) Micro-grafting medium: MS+BA 0.1mg / L+IBA 0.1mg / L+sucrose 40g / L;

[0062] (6) Rooting medium: 1 / 2MS+NAA0.1mg / L+white sugar 20g / L;

[0063] (7) Sterile seeding medium: 1 / 2MS + white sugar 20g / L.

[0064] 2), ginseng fruit stem tip detoxification culture:

[0065] (1) Selection and sterilization of explants: clip terminal buds from potted plants or field plants as explants, and use them as explants for detoxification tissue culture after sterilization;

[0066] ...

Embodiment 2

[0092] In this example, the basic medium: MS medium or 1 / 2MS medium, agar 7g / L, pH5.6; induction medium: MS+BA 0.5mg / L+IAA 0.05mg / L+ sucrose 30g / L ;Micro cutting medium: MS+BA 0.1mg / L+NAA0.05mg / L+sugar 30g / L; Strong seedling medium: MS+BA 0.05mg / L+NAA 0.01mg / L+PP 333 0.1mg / L+sugar 30g / L; micrografting medium: MS+BA 0.05mg / L+IBA 0.05mg / L+sucrose 40g / L; rooting medium: 1 / 2MS+NAA 0.05mg / L+sugar 20g / L; Sterile seeding medium: 1 / 2MS + 20g / L sucrose. During the detoxification culture of ginseng fruit stem tips, the terminal buds were taken as explants, soaked in 75% alcohol for 0.5-1.0 min, then sterilized with 0.1% mercuric chloride solution for 10 min, and finally rinsed with sterile water for 3-5 times. When cultivating aseptic seedlings of indicator plant tomato, seeds are sterilized by soaking in 75% alcohol for 0.5-1.0 min, then sterilizing with 0.1% mercuric chloride solution for 30 min, and finally rinsing with sterile water for 3-5 times. All the other steps and conditio...

Embodiment 3

[0094] In this example, the basic medium: select MS medium or 1 / 2MS medium, agar 9g / L, pH5.7; induction medium: MS+BA 2.0mg / L+IAA 0.5mg / L+sucrose; micro cutting Medium: MS+BA 1.0mg / L+NAA 0.5mg / L+sugar 30g / L; Strong seedling medium: MS+BA 0.5mg / L+NAA 0.1mg / L+PP 333 0.5mg / L+sugar 30g / L; micrografting medium: MS+BA 0.5mg / L+IBA 0.5mg / L+sucrose 40g / L; rooting medium: 1 / 2MS+NAA0.5mg / L+sugar 20g / L; Sterile seeding medium: 1 / 2MS + sugar 20g / L. During the detoxification culture of ginseng fruit shoot tips, the stems with axillary buds were taken as explants, soaked in 75% alcohol for 0.5-1.0 min, then sterilized with 0.1% mercuric chloride solution for 15 min, and finally rinsed with sterile water for 3-5 times. When cultivating aseptic seedlings of indicator plant tomato, seeds are sterilized by soaking in 75% alcohol for 0.5-1.0 min, then sterilizing with 0.1% mercuric chloride solution for 20 min, and finally rinsing with sterile water for 3-5 times. All the other steps and condi...

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Abstract

The invention relates to a rapid virus detection method of monorchid herminium herb virus-free seedlings of tissue culturing, which belongs to the technology field of plant virus detection, including the following steps: preparing the culture medium, stem tip virus-free culturing, virus-free seedling culturing of the indicator plant tomato, micrografting culturing and the proliferation culture of the virus-free seedlings of tissue culturing. The method has the advantages that the adopted culture medium, tissue culturing conditions and the micrograft cutter are applicable to the micrografting culturing of the virus-free seedlings and facilitating the healing of the graft unions with a grafting rate at more than 90%, which fully meet the requirement of the virus detection. Compared with the conventional field indicator plant detection, the method greatly reduces the required detection time, which is free from time and environmental factors and can be carried out under the laboratory conditions throughout the year. The method is very practical and applicable to the rapid virus detection of the monorchid herminium herb virus-free seedlings and the mass production of the virus-free seedlings.

Description

technical field [0001] The invention relates to the technical field of virus-free tissue culture and rapid propagation of plants, in particular to a virus rapid detection method for virus-free tissue culture seedlings of ginseng fruit. Background technique [0002] Ginseng fruit (Solamun muricatum Ait), also known as fragrant fruit, is an erect perennial herb or subshrub of Solanaceae, native to Peru and Ecuador in South America. Its nature likes warm, cool and dry climate, and the suitable temperature for growth is 18-20°C. Ginseng fruit is served as berries, low in sugar and fat, and can be fried, roasted, fried, made into soup, eaten fresh, etc. According to analysis, its fruit is rich in vitamins and various trace elements needed by the human body: copper, potassium, magnesium, selenium, etc. Eating ginseng fruit has a good preventive effect on various cancers, high blood pressure, diabetes, coronary heart disease, etc., and is known as the king of anti-cancer and gree...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G1/00A01G1/06A01G31/00C12N5/04
Inventor 陈剑平徐刚汪一婷吕永平牟豪杰
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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