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Method for in-vitro culture of stem apexes of sweet potatoes and virus detection of tissue culture seedlings

A virus detection and in vitro culture technology, applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., to achieve the effects of high virus detection accuracy, improved success rate, and effective detection methods

Inactive Publication Date: 2017-07-25
TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no varieties with complete resistance to the virus and a method of complete immunity, and there is no specific agent. The use of stem tip detoxification technology is still the fundamental method to prevent and control sweet potato virus diseases, restore good seed properties, and improve yield and quality.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A method for in vitro culture of sweet potato shoot tips and virus detection in tissue cultured seedlings, the specific steps are:

[0018] (1) Material collection and disinfection

[0019] Cut off the top buds of sweet potato seedlings growing vigorously in the field in June, cut off the leaves, rinse with tap water for 40 minutes, blot the water with sterile filter paper, put it on a clean workbench and soak it with 75% alcohol for 25 seconds, and then use 0.1% L Mercury disinfection for 5 minutes, finally rinsed with sterile water for 7 times, and set aside;

[0020] (2) Shoot tip stripping and cultivation

[0021] Under the binocular dissecting microscope, peel off the outer leaves of the sterilized sweet potato terminal buds with the tip of a syringe, leaving only one leaf primordium, and inoculate five shoot tips with a size of 0.1mm on the primary medium for cultivation. The medium sucrose is 30g / L, the agar is 6.0g / L, the pH is 5.8, the culture temperature is ...

Embodiment 2

[0027] A method for in vitro culture of sweet potato shoot tips and virus detection in tissue cultured seedlings, the specific steps are:

[0028] (1) Material collection and disinfection

[0029] Cut off the top buds of sweet potato seedlings growing vigorously in the field in June, cut off the leaves, rinse with tap water for 50 minutes, blot the water with sterile filter paper, place it on a clean bench and soak it with 75% alcohol for 25 seconds, and then rinse it with 0.1% L Mercury disinfection for 5 minutes, finally rinsed with sterile water 8 times, and set aside;

[0030] (2) Shoot tip stripping and cultivation

[0031] Under the binocular dissecting microscope, peel off the outer leaves of the sterilized sweet potato terminal buds with the tip of a syringe, leaving only 2 leaf primordia, and inoculate 6 shoot tips with a size of 0.2mm on the primary medium for cultivation. The medium sucrose is 30g / L, the agar is 6.0g / L, the pH is 5.8, the culture temperature is 25...

Embodiment 3

[0037] A method for in vitro culture of sweet potato shoot tips and virus detection in tissue cultured seedlings, the specific steps are:

[0038] (1) Material collection and disinfection

[0039] Cut off the top buds of sweet potato seedlings growing vigorously in the field in June, cut off the leaves, rinse with tap water for 45 minutes, blot the water with sterile filter paper, place it on a clean bench and soak it with 75% alcohol for 25 seconds, and then rinse it with 0.1% L Mercury disinfection for 5 minutes, finally rinsed with sterile water 8 times, and set aside;

[0040] (2) Shoot tip stripping and cultivation

[0041] Under the binocular dissecting microscope, peel off the outer leaves of the sterilized sweet potato terminal buds with the tip of a syringe, leaving only one leaf primordium, and inoculate 6 shoot tips with a size of 0.2mm on the primary medium for cultivation. The medium sucrose is 30g / L, the agar is 6.0g / L, the pH is 5.8, the culture temperature is...

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Abstract

The present invention is a kind of method for in vitro culture of sweet potato stem tip and detection of virus in tissue culture seedlings. The specific steps are: (1) material collection and disinfection: in June, cut the top buds of sweet potato seedlings growing vigorously in the field, cut off the leaves, Rinse with tap water, dry the water with sterile filter paper, and place it on an ultra-clean workbench for disinfection; (2) Stripping and culturing of shoot tips: Under a binocular dissecting microscope, peel off the outer surface of the sterilized sweet potato shoots with the tip of a syringe. (3) virus detection of tissue culture seedlings: virus detection was carried out by using the needle-pricking juice dipping method of indicator plants, and the virus-carrying seedlings were eliminated according to the detection results. Keep the virus-free seedlings. The invention provides a method for in vitro culture of sweet potato shoot tips and virus detection of tissue cultured seedlings. The success rate of sweet potato detoxification is improved, the accuracy of virus detection is high, and an effective detection means is provided for preventing and controlling sweet potato virus diseases.

Description

technical field [0001] The invention relates to the field of virus detection of sweet potato tissue culture seedlings, in particular to a method for in vitro culture of sweet potato shoot tips and virus detection of tissue culture seedlings. Background technique [0002] Sweet potato is a vegetatively propagated crop. Field planting is susceptible to infection by feather mottle virus (SPFMV), cauliflower mosaic virus (SPCLV), latent virus (SPLV) and tobacco mosaic virus (TMV), leading to species degradation. Reduced resistance, reduced yield and quality. The virus infection rate of field plants in most planting areas in my country is 60%-70%, and some even reach 90%, which leads to a 29% decrease in sweet potato production every year. At present, there are no varieties with complete resistance to the virus, no method of complete immunity, and no specific medicaments. The use of stem tip detoxification technology is still the fundamental method to prevent and control sweet p...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12Q1/70
CPCA01H4/001A01H4/008C12Q1/04
Inventor 王立国王刚李燕李建军高翔张嘉
Owner TIANJIN FENGHUA YULONG AGRI DEV CO LTD
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