80results about How to "Effective detection means" patented technology

The invention discloses a high temperature electrical capacitance tomography sensor and a production method thereof. The sensor can be applied to a 300 DEG C high temperature environment. The sensor includes high temperature-resistant array-distributed measuring electrodes, a shaft end shielding electrode, an insulation and isolation layer, an outer shield cover and a signal transmission line, wherein the array distribution electrodes comprise N measuring electrodes; and the signal transmission line comprises a high temperature section and a normal temperature section and comprises a cable core, an insulating layer and a shield silk screen. In the invention, the defect of no resistance of traditional sensors to a high temperature is improved, the problem of difficulty in arrangement of electrodes in the high temperature environment is solved, the electrodes and the outer shield cover are isolated and fixed by a high temperature-resistant insulating material, and signals of the measuring electrodes are led out through the high temperature signal transmission line, so an effective means is provided for researching gas and solid two-phase distribution, the fluidization characteristics and relevant reaction monitoring in the high temperature environment, and the application field of an electrical capacitance tomography technology is widened.

The invention relates to a safety detection forewarning device of running parts of a railway vehicle, and belongs to the technical field of safety detection of railway vehicles. The safety detection forewarning device solves the problem that manual detection for vehicles on railway is low in efficiency. The safety detection forewarning device comprises a vehicle wheel signal acquiring and distributing module, a vehicle number acquiring module, a sound acquiring and processing module, a temperature acquiring and processing module, an image acquiring and processing module, a power acquiring andprocessing module, a laser image acquiring and processing module, a main computer, a displayer and an alarm device. When the vehicle running on a railway line passes through the safety detection forewarning device, the safety detection forewarning device detects the vehicle in the following steps of: detecting the running part of the vehicle; enabling all acquisition modules to analyze and processthe acquired data to determine whether the working state of the parts of the detected vehicle is normal; and finally, combining analysis data from the modules to perform fault content forecasting. The safety detection forewarning device disclosed by the invention is mainly used for detecting vehicles passing through the device.

The invention firstly establishes an ultrahigh-efficiency liquid chromatography method which is used for quickly extracting and determining nine medicinal biogenic amines in the kudzu vine root whichis a medicine-and-food homologous product. The nine biogenic amines comprise tryptamine, phenylethylamine, putrescine, cadaverine, histamine, octopamine, tyramine, spermidine and spermine. After breaking a kudzu vine root sample, extraction by 0.4mol/L hydrochloric acid is utilized. Derivatization is performed in front of a dansyl chloride column. Ammoniacal liquor is added for terminating reaction, and acetonitrile is added for realizing a constant volume. A UPLC chromatographic column is utilized. Ammonium acetate and acetonitrile are used as mobile phase for performing gradient elution. A diode array detector is utilized for detecting in a wavelength of 254nm. The detecting method has an excellent linear relation. The sample recovery rate is 75.6-97.2%, and the relative standard deviation is 0.12-5.32%. The method limit of quantitation of the nine biogenic amines are respectively 10mg/kg. The method has advantages of simple operation, high speed and high sensitivity. The method canbe used for simultaneously measuring the residual amount of nine biogenic amines in the animal and botanical food such as kudzu vine root.

The invention discloses a human physiological feature selection algorithm based on combination of filtering and improved clustering. The algorithm includes S1, selecting an impedance model, collectingfirst feature parameters and second feature parameters to construct an initial feature set and a final optimal sub set; S2, introducing a filtering algorithm and applying the algorithm on each feature in the collected data; S3, ranking the feature set according to HSIC values from large to small; S4, adding features ranked first K into the feature set and filtering away body component unrelated parameters by utilizing the filtering algorithm, and constructing an initial data set; S5, constructing a feature sparse graph based on the data set according to the clustering algorithm; S6, utilizingthe improved clustering algorithm to screen redundancy features in the clusters. By adopting the algorithm provided by the invention, human body component predication precision can be improved, and amore effective detection means can be provided for human body component study and clinic application.

The invention discloses a high-throughput kit for simultaneously detecting three avian virus diseases, and a detecting method and the application thereof. A detecting kit for detecting NDV ((NewcastleDisease Virus), IBV (Infectious bronchitis Virus), AIV (Avian Influenza Virus) and H5, H7 and H9 subtype influenza virus nucleic acid thereof through a gene chip method provided by the invention hasa higher detecting speed, higher sensitivity and convenience in operation. The invention researches and develops a detecting kit for simultaneously detecting the NDV, the IBV, the AIV and the H5, theH7 and the H9 subtype influenza virus nucleic acid thereof, and can quickly diagnose whether chickens are infected with the detected viruses and monitor the morbidity situation of the chickens in practical production. A new and effective detecting means is provided for large-scale virus monitoring, epidemiological investigation, and prevention and control of the NDV, IBV, AIV and H5, H7 and H9 subtype influenza virus nucleic acid clinically.

The invention discloses a water body heavy metal semi-quantitative analysis method based on X ray fluorescent spectroscopy. The method comprises the following steps: a) drawing a calibration curve with a to-be-detected heavy metal standard liquid; b) collecting a water sample by adopting a water sample collector; c) analyzing the sample collected in the step b) by adopting an XRF spectroanalysis instrument; and d) calibrating a numerical value measured in the step c) by adopting the calibration curve obtained in the step a), so that heavy metal content of the water sample is obtained. The method disclosed by the invention has the advantages that the water sample is adsorbed to a piece of a solid adsorbent filter paper by using the water sample collector, and then an XRF instrument is used for detecting heavy metals attached to the filter paper, so that quick and simple determination on the heavy metals in a water body is realized, and a rapid and effective detection means is provided for environmental monitoring; the method is applicable to detection of the water sample which is polluted by multiple heavy metals such as mercury, lead, chromium, nickel and arsenic and is in a certain concentration range; and the method disclosed by the invention is based on an XRF method, the analysis sensitivity of the method is influenced by the XRF instrument, and calibration by virtue of a standard solution needs to be carried out before the method disclosed by the invention is used.

The invention provides a method for simultaneously determining the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid. The method comprises the following steps: extracting a to-be-tested sample by virtue of a polarity organic solvent under an ultrasonic condition, so as to obtain an extracting solution; and carrying out high performance liquid chromatography on the obtained extracting solution to obtain the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid in the to-be-tested sample according to standard curves of the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid and a liquid chromatogram of the to-be-tested sample. By utilizing the method provided by the invention, the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid can be simultaneously determined; and furthermore, the separation degree is good, the sensitivity and the simultaneously are high, the recovery rate is relatively high, and an effective detection measure is provided for a preparation utilizing the five active components as quality test indexes.

The invention discloses a method for quickly detecting Impatiens necrotic spot virus on oncidiums. The method uses Impatiens necrotic spot virus antiserum to detect the Impatiens necrotic spot virus on oncidium leaves which represent a concentric circle chlorosis spot symptom; a special primer ZI2R of the Impatiens necrotic spot virus is utilized to synthesize cDNA; the synthesized cDNA is applied, and a special primer ZI2F/ZI2R and a special primer ZI1F/ZI1R are respectively used to amplify to establish a RT-PCR assay. The method can also perform secondary amplification to a PCR product of ZI1F/ZI1R amplification respectively with ZI1F/ZI3R, ZI3F/ZI1R and ZI3F/ZI3R as inner primers to establish a nested PCR assay. The method has high sensitivity and good specificity, and provides an effective detection means for timely preventing and curing the attacking of the Impatiens necrotic spot virus to strains.

The invention discloses a quantitative detection method for simultaneously detecting three cat susceptible viruses and a primer probe combination thereof. The sequences of the primer probe combinationare respectively FPV-F, FPV-R, FPV-P, FHV-1-F, FHV-1-R, FHV-1-P, FCV-F, FCV-R and FCV-P. The multiplex fluorescence quantitative PCR method provided by the invention can be used for simultaneously detecting three cat viruses including FPV, FHV-1 and FCV; has high detection specificity and sensitivity and simple, convenient and rapid operation, can rapidly identify and diagnose whether the cat isinfected with the virus to be detected or not in a clinical sample, can quantitatively monitor the onset condition of the cat, and provides an effective detection means for clinical monitoring of FPV,FHV-1 and FCV of the cat, epidemiological investigation, and prevention and control of the diseases.

The invention relates to a method for measuring trace triptorelin. The trace triptorelin in a sample is measured by using a liquid chromatography and mass spectrometry technology. The method is simple in operations and accurate in results, and has good specificity and high sensitivity. The invention also relates to applications of the measurement method for the qualitatively or quantitatively measuring the content of the triptorelin in a biological sample and for pharmacokinetic studies of triptorelin preparations.

The invention provides real-time fluorescent quantitative PCR nucleic acid sequences and a kit for detecting Clostridium botulinum type A. The nucleic acid sequences comprise an upstream primer A-F, adownstream primer A-R and a probe A-P. As the primers and the probe provided by the invention are used for detecting Clostridium botulinum type A, detection accuracy reaches 100%; and when the primers and the probe are used for amplification of other 25 intestinal pathogenic bacteria and common bacterial DNAs, no specific amplification curve appears, so the primers and the probe have good specificity. According to a fluorescence quantitative PCR standard curve constructed on the basis of a recombinant plasmid standard substance, it is determined that the detection sensitivity to Clostridium botulinum type A is 5.04*10<2> copies/[mu]l. In a fluorescent quantitative PCR detection system, when the concentration of a recombinant plasmid containing the A toxin gene is in the range of 10<2>-10<9> copies/[mu]l, the Ct values of amplification reactions and template concentrations are in good linear relationship. The real-time fluorescent quantitative PCR nucleic acid sequences and the kit ofinvention have the characteristics of sensitive reaction, high specificity, rapidity, convenience, high throughput, etc., and provide an effective detection means for screening, large-scale quarantineand epidemiological investigation of Clostridium botulinum type A.

The invention belongs to the field of virus and epidemic disease diagnosis and animal quarantine, particularly relates to an African swine fever virus p72 recombinant protein, and further discloses colloidal gold immunochromatography test paper constructed based on the recombinant protein and a method for detecting an African swine fever virus antibody. According to the colloidal gold test paper based on African swine fever virus p72 protein double-antigen sandwich, a recombinant antigen of ASFV strong immunogenicity main structural protein p72 is marked by colloidal gold, and ASFV antibody in porcine serum is detected in a double-antigen sandwich mode. According to the double-antigen sandwich colloidal gold immunochromatography test paper, after a sample is collected, the sample is mixed with a sample diluent according to a certain proportion and then dropwise added into a sample hole, a detection result can be obtained within 5 minutes, the result is accurate, the double-antigen sandwich colloidal gold immunochromatography test paper can be directly used for detecting a virus antibody in suspicious porcine serum, and the double-antigen sandwich colloidal gold immunochromatography test paper is especially suitable for on-site ASFV serological diagnosis, epidemiological investigation and swine international trade quarantine inspection.

The invention discloses an electromagnetic wave propagation speed based biological tissue electromagnetic parameter imaging device and method and belongs to the field of biological tissue electromagnetic parameter imaging. The electromagnetic wave propagation speed based biological tissue electromagnetic parameter imaging device comprises an electromagnetic wave transmitting coil, an electromagnetic wave receiving coil, a U-shaped arm, an objective table, a motor unit, a motor driving unit, a control unit and an opening type oscillating circuit. The electromagnetic wave propagation speed based biological tissue electromagnetic parameter imaging method comprises initializing devices; measuring the electromagnetic wave propagation speed of biological tissues; enabling an FPGA (Field Programmable Gate Array) to upload the electromagnetic wave propagation time to a terminal computer in real time through a single chip microcomputer, enabling the terminal computer to calculate the electromagnetic wave propagation speed of positions of the biological tissues through a speed formula according to the distance between the electromagnetic wave transmitting coil and the electromagnetic wave receiving coil; performing image reconstruction on the biological tissue through a regularization Gaussian Newton reconstruction algorithm (NOSER) and obtaining a reconstruction image of the biological tissue. The electromagnetic wave propagation speed based biological tissue electromagnetic parameter imaging device and method provides a brand new, simple and effective detection mean and is low in cost and free of radiation compared with traditional medical imaging equipment.

The invention relates to the field of tumor diagnosis, and discloses establishment of a composition for early diagnosis of ovarian cancer, ovarian cancer can be predicted, diagnosed and/or prognostically evaluated by measuring cfDNA content, cfDNA TP53 mutation abundance and CA125 protein expression level, the sensitivity, specificity and accuracy of the composition reach 91.11%, 94.34% and 93.38%, and the detection rate of early ovarian cancer reaches 78.9%. By adopting the marker combination and the model scoring method disclosed by the invention, 71.05% of patients can be detected in CA125 negative ovarian cancer patients, 74% of subjects can be correctly judged in CA125 positive non-tumor people, and the problems of CA125 leak detection and error detection can be well solved. According to the invention, an effective early ovarian cancer diagnosis or screening marker can be provided clinically, and the prediction accuracy and the detection rate of the early ovarian cancer can be improved.

The invention discloses serum markers for preoperatively detecting liver cancer microvascular invasion. A reverse serum-protein omics technology is adopted to in vivo study associated antigens and antibodies of liver cancer MVI for the first time, the serum markers for preoperatively detecting liver cancer microvascular invasion are determined, and an effective detection means is provided for clinical preoperative early diagnosis of MVI.