Method for simultaneously determining content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid
A technology of cornel glucoside and oleanolic acid, which is applied to the content of loganin, oleanolic acid and ursolic acid, and simultaneously determines the field of morroniside and cornel glucoside, which can solve the problems of poor water solubility and large differences in structure and properties , Increased difficulty and other issues, to achieve the effect of high repeatability, good separation and high sensitivity
Active Publication Date: 2017-05-17
天津市第一中心医院
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Problems solved by technology
But, oleanolic acid and ursolic acid are a pair of isomers close in structure, and water solubility is extremely poor, when acetonitrile and 0.1% phosphoric acid are mobile phase gradient elutions, two peaks overlap and can't be separated; Simultaneously, The structure and properties of oleanolic acid and ursolic acid are quite different from those of morroniside, loganin and cornus, which further increases the difficulty of simultaneous detection of the contents of these five active ingredients
In the prior art, there is no report on simultaneous detection of the content of these five active ingredients
Method used
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Embodiment 1
[0072] (1) Establishment of standard curve
[0073] The reference substances morroniside (batch number 20150617), cornelin (batch number 20140529), loganin (batch number 20150112), oleanolic acid (batch number 20150826) and ursolic acid (batch number 20150727) were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Co., Ltd., the quality scores are all ≥98.0%.
[0074] Accurately weigh 1.6, 3.6, 1.4, 1.6, and 2.0 mg of morroniside, loganin, cornelin, oleanolic acid, and ursolic acid reference substances, respectively, and use ethanol as a solvent to prepare 1.2 mL of mixed reference substances solution; the resulting mixed reference substance solution is diluted in multiples to obtain mixed reference substance solutions of different dilution concentrations, and the mass concentrations of morroniside in the mixed reference substance solution are respectively 1333.33, 666.67, 333.33, 166.67, 83.33, 41.67, 20.83, 10.42mg / L, the mass concentrations of loganin were 3000.00, 15...
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Abstract
The invention provides a method for simultaneously determining the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid. The method comprises the following steps: extracting a to-be-tested sample by virtue of a polarity organic solvent under an ultrasonic condition, so as to obtain an extracting solution; and carrying out high performance liquid chromatography on the obtained extracting solution to obtain the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid in the to-be-tested sample according to standard curves of the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid and a liquid chromatogram of the to-be-tested sample. By utilizing the method provided by the invention, the content of morroniside, loganin, cor-nuside, oleanolic acid and ursolic acid can be simultaneously determined; and furthermore, the separation degree is good, the sensitivity and the simultaneously are high, the recovery rate is relatively high, and an effective detection measure is provided for a preparation utilizing the five active components as quality test indexes.
Description
technical field [0001] The invention relates to the technical field of determination of active ingredients, in particular to a method for simultaneously determining the contents of morroniside, loganin, cornus glucoside, oleanolic acid and ursolic acid. Background technique [0002] The hepatoprotective agent is a preparation extracted from the traditional Chinese medicinal material Cornus officinalis, and its main active ingredients are morroniside, loganin, cornuside, oleanolic acid and ursolic acid. Among them, morroniside, loganin and cornus new glycosides belong to the iridoid glycosides (Iridoids), which have the functions of lowering blood sugar and lipids, protecting the liver and gallbladder, anti-tumor, anti-virus, anti-bacterial, anti-oxidation and regulating cardiovascular System and digestive system and other pharmacological activities; oleanolic acid and ursolic acid belong to organic acid compounds, which are a pair of pentacyclic triterpenoid isomers with si...
Claims
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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 刘宏胜王树森张雅敏徐新女刘涛
Owner 天津市第一中心医院
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