169 results about "Gene technology" patented technology

The invention relates to a syphilis serological screening or diagnosis method. The purpose is that the method has the characteristics of low price, high speed, sensitivity and specificity. The technical scheme provided by the invention is that: a preparation method of rTpN15-17-47-ELISA for detecting syphilis serum antibodies comprises the following steps in turn: constructing an artificial fusion gene tpN15-17-47 of tpN15, tpN17 and tpN47 and a prokaryotic expression system E.coliBL21DE3Pet42a-tpN15-17-47 by gene technology, purifying the recombinant expression product rTpN15-17-47 as a coating antigen, establishing rTpN15-17-47-ELISA for detecting syphilis serum antibodies. The fusion gene tpN15-17-47 has a nucleotide sequence and an amino acid sequence as shown in sequence table 5.

The present invention belongs to the technical field of cell biology and particularly relates to a technology for regulating and controlling a Jak-Stat pathway to differentiate, dedifferentiate and rejuvenate cells, and an application of the technology. Rejuvenated cell products and/or cell products of different lineage types are obtained by small molecule compound combination, cytokine combination or recombinant protein combination, a gene editing technology and a transgenic technology to quantitatively and/or regularly regulate and control gene or protein targets of the Jak-Stat signaling pathways in cells. The provided technology for regulating and controlling the Jak-Stat signal pathway, and the product/derivatives of the cell product can be used for re-programming cells, tissues, organs and organisms, construct tissue engineering materials, repair damages of tissues and organs of mammals and the aged and degenerated tissues and organs, delay or reverse aging processes of the cells, tissues, organs and organisms, and can be used for immunoregulation of the cells, tissues, organs and organisms.

The invention discloses a strain of brew yeast and application of the brew yeast to manufacturing alcohol through fermentation. The brew yeast, (Saccharomyces cerevisiae) SC-X234, is collected on January 6th, 2014 at China Center for Type Culture Collection (CCTCC), with the collection number of CCTCC M2014005; the feeble train N<+> infusion mediate spt15 gene technology is adopted, SC-100 is used as initiation strain; primary screening is conducted by utilizing a TTC slab and an alcohol slab; the obtained strain is subjected to fermentation and secondary screening to gradually increase the tolerance of the yeast in alcohol; the manufacturing strain SC-X234 with the yield of 15.75% can be finally obtained; the strain can greatly increase the fermentation productivity; the sugar to alcohol conversion ratio is high; the alcohol yield in a 5 L fermentation tank can reach 126 g/L, which is 87.5% higher than that of the initiation strain; the alcohol has a very high economic value as a fuel alcohol.

The invention discloses a beta-glucosaccharase gene, wherein the nucleotide sequence of the beta-glucosaccharase gene is shown as the SEQ ID NO.1. The invention further discloses recombinant beta-glucosaccharase, wherein the amino acid sequence of the recombinant beta-glucosaccharase is shown as the SEQ ID NO.2. The invention also discloses a screening method of the recombinant beta-glucosaccharase. The screening method comprises the following steps: extraction of microbial metagenomic DNA from the mildewed sugarcane leaves; establishment of a metagenomic library; screening of the beta-glucosaccharase gene from the metagenomic library, wherein the nucleotide sequence of the beta-glucosaccharase gene is shown as the SEQ ID NO.1; and cloning and expression for the obtained beta-glucosaccharase gene, thus the recombinant beta-glucosaccharase is obtained, wherein the amino acid sequence of the recombinant beta-glucosaccharase is shown as the SEQ ID NO.2. The recombinant beta-glucosaccharase has extremely high activity and is not sensitive to hydrolysis products under the alkaline condition.

The present invention belongs to the field of molecular biology and gene technology, and is especially the nucleotide coding sequence of flavoid-3', 5'-hydroxylase gene expressed in butterfly orchid, and its application in changing flower color. The present invention relates to the recombinant expression vector including the said gene, the transgenic plant, nucleotide primer sequence for obtaining the gene, the oligonucleotide sequence probe for detecting endogenous expression mode and method of analyzing and identifying the expression mode of the endogenous gene in butterfly orchid. Transforming the gene into petunia obtains transgenic plant with differentially expressed flavoid-3', 5'-hydroxylase gene and altered flower color.

The invention relates to the gene technology and medicine field, and in particular, relates to a method for predicting prognosis based on combined analysis of rectal cancer transcriptome genes and methylation and a model application. The invention provides an application of differentially expressed genes and differentially methylated genes in prognostic risk prediction of rectal cancer. The six special differentially expressed genes are TAMM41, AC022387.1, AP004609.1, GVQW3, AC245060.2 and AC027644.1. The seven special differential methylated genes are MIR7848, LRRC74B, MRPL13, SNRPD3, EMILIN2, NACAP1 and C8or87. The better choice is provided for early screening of rectal cancer, the accuracy of screening is improved, the mortality rate and local recurrence rate of rectal cancer patients are reduced, individualized and precise treatment is realized through the risk prediction model, and the prognosis of the patients is improved.

The invention belongs to the technical field of network communication security of the Internet of Things (IoT), and particularly relates to a malicious network behavior identification method and device based on the software gene technology. The malicious network behavior identification method based on the software gene technology comprises the following steps: constructing a network gene bank forterminals and the cloud; and carrying out malicious network behavior identification based on the network gene bank. The malicious network behavior identification device based on the software gene technology comprises a terminal and cloud network gene bank construction module, and a malicious network behavior identification module, wherein the terminal and cloud network gene bank construction module is used for constructing the network gene bank for the terminals and the cloud; and the malicious network behavior identification module is used for carrying out the malicious network behavior identification based on the network gene bank. The malicious network behavior identification method and device based on the software gene technology provided by the invention has the advantages that the gene detection efficiency is improved, and high IoT security is guaranteed.

The invention belongs to the field of gene technology and medicine, in particular to C9orf139 and MIR600HG as prognostic markers of pancreatic cancer and an establishment method of the markers. The pancreatic cancer prognosis markers comprise C9orf139 and MIR600HG. By exploring the differentially-expressed mRNA, lncRNA and miRNA in pancreatic cancer, a ceRNA network is constructed to find new molecular screening biomarkers for pancreatic cancer and provide new analytical and molecular mechanisms for lncRNAs, miRNAs and pancreatic cancer mRNA. The invention determines two new biomarkers as potential pancreatic cancer prognosis indicators, and thus the prognosis of pancreatic cancer diseases is improved, and the accuracy of early diagnosis of pancreatic cancer is improved.

The invention relates to an improved cotton fiber color gene technology, and particularly relates to a cotton gene and a plant expression binary vector constructed by the gene and application to improvement of cotton brown fiber color. A nucleotide sequence of the cotton GhMATE1 gene is shown in SEQ ID: NO.1. An amino acid sequence of a protein coded by the gene is shown in SEQ ID: NO.2. On the basis of an early-cloned cotton GhTT12a gene (Chinese patent application number: 201310380270.5, and data of application: 2013-8-27), by using a bioinformatics analysis and electronic cloning method, a new gene containing an MATE (multidrug and toxic compound extrusion) conserved domain database is cloned, but a gene structure and a shearing mode of the cotton GhMATE1 gene are completely different from those of the GhTT12a gene, and the cotton GhMATE1 gene has 78 percent of sequence homology with a nucleotide sequence of an arabidopsis thaliana testa brown pigment synthetic TT12 (Transparent Testa) gene.

The present invention relates to gene technology field, and is gene diagnosis chip for individual administration and its making process and usage. The gene diagnosis chip includes bearing substrate and gene probes distributed on the substrate, and the gene probes contain oligonucleotide sequences complementary with the medicine metabolism and effect related gene mutant sites and have reference probes set. Each mutant site has two types of probes, wild type and mutant type, designed. The chip making process includes designing two types of probes of length 17-22 bp for each mutant site, synthesizing probes with DNA sequence synthesizing instrument, applying probes onto the substrate with chip sample applying instrument, curing the chip and storing at 4 deg.c. The usage of the gene diagnosis chip includes sampling, sample processing, PCR amplification, crossbreeding, washing, reading, result analysis and other steps.

The invention discloses an ultrahigh permeability bread yeast and a preparation method thereof, and relates to a pattern biological bread yeast, which can be used for detecting environmental genotoxic carcinogen. Through reconstruction of genetic gene technology, the yeast has improved permeability, so that the yeast has wider detection range and higher sensitivity to environmental pollutants. Through gene knock-out technology, genes CWP1 and CWP2, which are used for encoding protein mannose protein serving as an important structure of bread yeast cell wall, is inactivated to improve the permeability of the bread yeast cell wall to environmental carcinogen; and genes SNQ2 and PDR5, which are used for encoding an efflux pump protein on a cell membrane of bread yeast, is inactivated to improve the accumulation of the environmental pollutants in the bread yeast cell. Through the integration of two reconstruction strategies, an RNR3-1acZ system is constructed when four genes CWP1, CWP2, SNQ2 and PDR5 knock out bread microzyme, which can improve the sensitivity of the system to detecting the environmental genotoxic carcinogen.

The present invention relates to the field of biological gene technology detection, and specifically relates to a method and a kit for detecting mutational load of human genome based on high-throughput sequencing. The method for detecting the mutational load of the human genome based on high-throughput sequencing is as follows: extracting a genomic DNA double stranded nucleic acid sample from a blood and tissue sample, loading the genomic DNA double stranded nucleic acid sample onto a machine for sequencing the determination area 0.8-2.4Mb of the sample to obtain a nucleic acid sequence of the blood and tissue sample, performing automatic processing on the obtained nucleic acid sequence, and calculating the number of mutation sites in the tissue sample according to the formula of mutational load = the total number of mutations in the determination area of the sample / the size of the determination area of the sample to obtain the number of the mutational load. The specific area is selected for target sequencing, double samples are used for paired detection, sequencing depth is simultaneously increased, sequencing accuracy and sensitivity can be improved, and the required amount of data for the sample is small. The present invention also provides the kit for detecting the mutational load of the human genome used for the method.

The invention provides a gene and polypeptide for regulating and controlling plant height of crop and an application of the polypeptide and belongs to the fields of gene technology and botany. The sequence of the gene is any one of SEQ ID NO: 1-6. According to the gene provided by the invention, the plant height of crops can be remarkably regulated, so that the plant height of rice and other crops can be better controlled, the yield is guaranteed, and the aim of stable output and high yield can be achieved. The gene provided by the invention has important application value to crop improvementand breeding.

Belonging to the field of gene technology in molecular biology, the invention relates to a betula platyphylla BplSPL1 gene for promoting precocious flowering and an encoded protein thereof. The invention is characterized in that the betula platyphylla BplSPL1 gene for promoting precocious flowering has a nucleotide sequence shown as SEQ ID NO:1 in a sequence table, and has an amino acid sequence shown as SEQ ID NO:2. The invention provides the betula platyphylla flowering gene BplSPL1 and applies the gene to construction of a BplSPL1 gene's plant expression vector, and the constructed plant expression vector is subjected to agrobacterium impregnation of an arabidopsis thaliana plant so as to obtain a transgenic arabidopsis thaliana plant and achieve precocious flowering. Therefore, the gene has the function of inducing precocious flowering of arabidopsis thaliana and can regulate plant leaf development. The gene provided by the invention utilizes genetic engineering technology to regulate betula platyphylla flowering time and leaf development, provides gene resources and theoretical basis, and has great application value.

The invention belongs to the field of gene technology and medicine, and particularly relates to a marker for predicting prognosis of colon cancer and application of the marker. The marker for predicting prognosis of the colon cancer comprises autophagy-related lncRNA and/or autophagy-related mRNA markers, wherein the autophagy-related lncRNA is CASC9, PCAT6, AP006621.2, GS1-124K5.4, MIR4435-2HG, AL354993.2, AC048344.4, AC010973.2, AL590483.1, AL137782.1, STAG3L5P-PVRIG2P-PILRB, LINC00513, SNHG16 and AP001554.1; and the autophagy-related mRNA is GRID1, DAPK1, RAB7A, PELP1, ULK3 and WIPI2. The marker for predicting the prognosis of the colon cancer can be used for predicting the prognosis risk and survival rate of the colon cancer, provides a guidance basis for prognosis and health management of patients, and realizes individual accurate treatment.

The invention discloses a biosynthesis gene cluster of Citrinin, which belongs to the microorganism gene technology area. The invention provides ten genes which are related to the biosynthesis Citrinin. The genes are nine modification genes of Citrinin which are ctnD, ctnE, ctnF, ctnG, ctnH, ctnI, orf2, orf3, orf4 and a regulation gene of Citrinin which is ctnR1. The genes, proteins and the antibodies provided by the invention can be used for the detection and development of the compound and protein which are used in medicine, industry and agriculture areas. The invention also provides a way for blocking the biosynthesis of Citrinin.

The invention provides a gene for controlling the plant height and neck length of spike of rice and application, belonging to the technical field of plant genetic engineering and relating to the technical fields of plant transgenic technology and rice molecular breeding. A target mimic gene MIMb which weakens the eui (elongated uppermost internode)-1 gene interference function of artificial microRNA is obtained by the plant gene cloning technology. The nucleotide sequence of the target mimic gene MIMb is shown in SEQ NO:5 in a sequence table. The rice material with plant height and neck length of spike recovered to the wild levels is obtained by hybridizing the rice materials eZS97A and MH63-MIMb with long necks of spike. The invention also discloses a preparation method and application of the gene.

The invention belongs to the field of gene technology and discloses a fusion gene data detection method based on big data, a computer program and a terminal. The known fusion gene breakpoint, N sequences in front of the breakpoint and M sequences behind the breakpoint are combined to form a seed, and the seeds are assembled to form a fusion gene database. The RNA of the gene to be detected is extracted by a kit; cDNA of the gene to be detected is obtained by reverse transcription of the extracted RNA by using a reverse transcription kit. The fusion gene is amplified by polymerase chain reaction using cDNA as template. Gene information to be detect is obtained by detect a gene fragment to be detected by a gene detection technology. The invention can effectively improve the gene sequence alignment efficiency and the user experience by comparing the sequences to be aligned according to the judgment result.

The invention discloses an application of a gene OsPIL16 in simultaneously improving tolerance of rice to drought stress and salt stress. In the application, a cDNA segment of the gene OsPIL16 of Nipponbare rice is adopted as an applied gene, the applied gene is transformed into rice in a forward direction, the expression level of the gene OsPIL16 is improved, and the genetically-modified positive rice plant has the salt tolerance and drought tolerance both improved. Under the salt stress (200mM NaCl) condition, the survival rate of a reference material is 1.3%, while the survival rate of genetically-modified rice seedlings is 68-75%, which is obviously higher than that of the reference material; under the drought (20% PEG4000) condition, the survival rate of the reference material is 18%, while the survival rate of the genetically-modified rice seedlings is 81-87%. With the adoption of the application provided by the invention, a new thought is provided for culturing rice varieties with multiple resistances and high resistances, and a theoretical basis is also provided for improving the stress resistance of other crops by utilizing the heterologous gene technology.

The invention relates to the field of gene technology and biomedicine, in particular to a method of for establishing and verifying a breast cancer prognosis evaluation model based on seven special genes. The invention provides the seven genes related to survival in breast cancer development and the method of for establishing and verifying the breast cancer prognosis evaluation model based on the seven special genes. The seven genes related to survival in breast cancer development comprise LYVE1, CD209, TMEM190, TUBA3D, AL049749.1, AC123595.1 and LILRB5. The model can correctly evaluate breastcancer onset risks, accurately predict patient prognosis and guide clinical treatment. Through transcriptome data and clinical data analysis, it is found that prognosis is more accurate through jointprediction via the seven genes, and prognosis evaluation of a patient can lay a foundation for clinical transformation through a nematic diagram.

The invention discloses a molecular and genetic engineering technology for cultivating colorful fluorescent pearls, and relates to a technology for cultivating bright-colored pearls and fluorescent pearls. The preparing technology includes the steps that a chemical self-assembled technology is used, natural dye molecules (algas rich in natural dye molecules are selected to be used as feed) are led in during the process of pearl forming, self-assembling is achieved on organic matrixes of pearl layers, and therefore the purpose of dying the pearls which are finally formed is achieved. Natural dye is used for conducting dyeing on the pearl layers, so that various colorful pearls can be achieved in a controllable mode. Through the modern genetic technology, fluorescent protein which is used widely in the biological chemistry scientific researches is applied to pearl oyster mantle tissue, and therefore the pearls with various fluorescent effects can be produced.

Belonging to the fields of functional material loaded drugs and gene technologies, the invention discloses a composite material of a functional mesoporous silica loaded drug and siRNA, a preparation method and application thereof in preparation of anticancer drugs. The composite material is prepared by the method comprising the steps of: adding mesoporous silica into a 3-mercaptopropyl trimethoxysilane solution, conducting heating reaction and separation to obtain thiolated mesoporous silica, dispersing the thiolated mesoporous silica into a 2, 2-dithiodipyridine solution, and carrying out heating reaction to obtain disulfide bond modified mesoporous silica, adding the disulfide bond modified mesoporous silica into a doxorubicin water solution, then adding thiolated siRNA, and conducting separation, thus obtaining the composite material of the functional mesoporous silica loaded drug and siRNA. The composite material provided by the invention can be applied to preparation of anticancer drugs, can load drugs into cells, significantly improves the enrichment amount of drugs in cells, plays a good mesopore blocking role, and reaches the effect of silencing gene when released in cells.

The invention belongs to the field of ornamental plant transgenic technology, particularly relates to a method for culturing transgenic dianthus and more particularly discloses a method for culturing transgenic dianthus by taking agrobacterium-mediated embryogenic callus of the dianthus as a material. The method comprises the following steps of embryogenic callus induction, genetic transformation, screening and regeneration, GUS (glucuronidase) coloration detection and PCR (polymerase chain reaction) detection. The method is characterized in that the GUS report gene and the ACO target gene are introduced into the receptor which is the embryogenic callus of the ianthus through agrobacterium mediation, different factors affecting the embryogenic callus transformation are screened, kanamycin or hygromycin is used as a screening agent to screen the transformed embryogenic callus, and the screening is carried out through GUS coloration, PCR detection and other auxiliary methods to obtain the transgenic dianthus. The invention provides a novel technology platform for the innovation of the germplasm resources for the genetic transformation of the dianthus.

The invention relates to a method and a kit for detecting genotype of thiopurine methyltransferase (TPMT), belonging to gene technology field. The method comprises the following steps of: performing polymerase chain reaction to a sample DNA using specific primers of TPMT gene polymorphism sites, digesting and amplifying the product with restriction endonuclease, and determining genotype according to the length polymorphism of the digested fragments, wherein the primers at least include a specific primer for detecting the TPMT G460A polymorphism site and a specific primer for detecting the A719G polymorphism site. The inventive method and the kit for genotype detection can provides guidance for the individualized administration of purine drugs; and has the advantages of good stability and reliability, low price, rapidness, and applicability to clinic application.

The invention belongs to the technical field of genes and especially relates to application of a gene Loc_Os01g12810. The gene Loc_Os01g12810 can be applied to rice chloroplast development regulationmechanism researches; a gene method is utilized to regulate an expression level of the gene Loc_Os01g12810 to research rice chloroplast development regulation mechanisms; a nucleotide sequence of thegene Loc_Os01g12810 is shown in SEQ ID NO:1 in a sequence table; an amino acid sequence of encoding protein of the gene Loc_Os01g12810 is shown in SEQ ID NO:2 in the sequence table. According to the application of the gene Loc_Os01g12810 disclosed by the invention, that the rice gene Loc_Os01g12810 is a gene related with rice chloroplast development is proved for the first time. The Loc_Os01g12810is a PPR gene. Cloning and biological function verification of the gene have important reference significance in rice chloroplast development molecule mechanism researches and researches on effects of PPR family gene in rice development.

The invention relates to a novel recombinant lactobacillus resisting diabetes mellitus type 1 and application thereof and belongs to the field of biomedicine and discloses a diabetes mellitus type 1 resistant vaccine using Lactococcus lactics as a vector and apreparation method and application thereof. A recombinant Lactococcus lactics vaccine using Lactococcus lactics as a vector and induced to express fusion protein HSP65-6IA2P2 is built by using a micro gene technology platform of the lab. The correct expression of a fusion protein vaccine is detected by Western blot analysis. In-vivo animal tests show that the vaccine can be orally administered to give significant reduction in the occurrence of diabetes in NOD (non-obese diabetic) mice and to prevent diabetes mellitus type 1, and has a promising clinical application prospect in terms of preventing diabetes.