42 results about "Gene technology" patented technology

The invention belongs to the technical field of genes, and discloses a method for efficiently expressing foreign protein based on an NC048599.1 site in a CHO cell genome. A site for stably expressing a protein in a CHO cell genome is located in a range of 69195000 to 69199000 of a CHO cell gene NC048599.1 with a sequence as shown in SEQ ID NO: 1 (Sequence Identifier Number 1), and the sequence of the CHO cell gene NC048599.1 is shown in SEQ ID NO: 1. Different protein genes are introduced at fixed positions in a CHO cell genome, and stable expression is carried out.

The invention discloses a transcription factor TgWRKY65 of torreya grandis as well as a coding gene and application of the transcription factor TgWRKY65. The amino acid sequence of the transcription factor TgWRKY65 is shown as SEQ ID NO.2, and the nucleotide sequence of the coding gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor TgWRKY65. According to the invention, a homologous expression material is constructed through cloning of the TgWRKY65 gene, a transgenic technology and gene silencing, and the application prospect in the aspect of increasing the content of the torreya grandis catechin is great; when the coding gene is over-expressed, the content of catechin is increased; when the coding gene is silenced, the content of catechin is reduced.

This invention belongs to the field of gene technology for disease control. This invention provides a method for preparing biocontrol engineered bacteria using *Trichoderma harzianum* as a dsRNA vector, comprising the following steps: determining the target gene based on the pathogenic fungus; using the target gene as an arm sequence, and centering on the VdTublin intron sequence of the *Verticillium dahliae* endogenous gene, connecting two identical arm sequences to both ends of the intron in a forward and reverse direction, respectively, to construct the target sequence; and transferring the target sequence into *Trichoderma harzianum* using ATMT to obtain engineered *Trichoderma harzianum* bacteria. The engineered strain obtained by this invention has a good inhibitory effect on the growth and pathogenicity of the corresponding fungus.

The invention relates to a rice gene OsMYB63L and application of the rice gene OsMYB63L in regulation and control of spike branching and yield, and belongs to the technical field of plant gene breeding, the nucleotide sequence of the gene OsMYB63L is as shown in SEQ ID NO.1, the amino acid sequence of protein coded by the gene is as shown in SEQ ID NO.2, overexpression of the gene OsMYB63L promotes development of the spike branching of rice, then the yield of rice is increased, a transgenic technology is utilized, and the yield of the rice is increased. Overexpression is carried out on the rice gene OsMYB63L, and a rice plant with increased ear branch number and increased yield can be obtained. The invention discovers and verifies that the rice gene OsMYB63L has the function of regulating and controlling the ear branching number and the yield of rice. And a new gene resource and a technical path are provided for molecular breeding of high-yield rice.

The invention provides a gene for regulating and controlling the shape of a tomato leaf and application thereof, and a construction method of a recombinant expression vector is characterized in that an SlPUB26 gene is inserted into a vector containing a strong promoter by utilizing an overexpression transgenic technology. Transgenic plants with different gene expression level up-regulation multiples are obtained through agrobacterium tumefaciens-mediated transformation, so that single plants with different leaf phenotype change degrees are obtained. According to the method, a precise targeting sequence does not need to be designed, the miss-target hidden danger does not exist, and single plants with different SlPUB26 gene expression levels can be obtained only by detecting the gene expression quantity, so that the single plants with different leaf shape change degrees are obtained. The technical operation difficulty is low, and the method is more suitable for non-professional molecular breeding teams or grass-roots agricultural scientific research institutions. The higher the up-regulation multiple of the expression level of the SlPUB26 gene is, the lower the length-width ratio of the tomato leaf is, so that the shape of the tomato leaf can be quickly and accurately adjusted by adjusting the expression level of the SlPUB26 gene.

The invention discloses an FvePREs transcription factor and application thereof in promoting curling of strawberry petals, in a general agricultural cultivation technology, the petals are curled by gibberellin (GA3) or a growth regulator for actively inducing the curling of the petals, and excessive hormone may cause malformation and curling of the petals and also may cause side effects such as fruit hollowness, quality reduction and the like. According to the scheme, the gene technology is ingeniously utilized to directly act on a plant endogenous regulation and control system, the synergistic effect of strawberry petal curling and quality optimization is achieved by activating or inhibiting specific gene expression (such as cell division and nutrient distribution), and in addition, the over-expression FvePREs transcription factor can be used for improving the yield of strawberry petals by changing the hereditary characteristics of strawberries. The good petal curling effect can be kept in the long-term planting process, dependence on external chemical substances can be reduced, and sustainable agricultural development is facilitated.

The invention relates to the field of gene technology application, in particular to a tomato salt stress gene SlGLUB, a primer and application of the tomato salt stress gene SlGLUB. In the invention, the SlGLUB gene can promote the root development of the tomato under normal conditions, and after salt stress treatment, the SlGLUB gene can improve the salt tolerance of the tomato by adjusting the root development of the tomato plant and increasing the total root length and the root surface area, and from the result of paraffin sections of roots, stems and leaves, the salt tolerance of the tomato can be improved on the cellular level. The SlGLUB gene can reduce the damage of salt stress to tomato plants, the SlGLUB gene can improve the capability of removing active oxygen by improving the activity of antioxidant enzyme, and the membrane lipid peroxidation degree can be reduced, so that the salt resistance of the tomato plants is improved.

The invention relates to the technical field of biological genes, in particular to a gene directed editing system applied to mycoplasma bovis, which is an editing system for expressing a combination of a single plasmid of GP35 single-chain annealing protein and a targeting fragment ssDNA (single-stranded deoxyribonucleic acid), and comprises an expression plasmid of a GP35 gene and the ssDNA of the targeting fragment. The invention relates to a gene directed editing method applied to mycoplasma bovis. The gene directed editing method comprises the following steps: constructing a mycoplasma bovis strain for expressing GP35 single-chain annealed protein, constructing an ssDNA single chain of a targeting fragment, and performing gene editing on a target gene to obtain a pdhC gene deleted strain. According to the invention, a KanR gene is successfully inserted at the 158bp position of a pdhC gene coding region of a mycoplasma bovis PG45 strain, so that a mycoplasma bovis pdhC gene oriented deletion strain is obtained. According to the invention, the metabolism-related gene directed deletion strain M. bovis PG45 [delta] pdhC is successfully constructed, and the pdhC protein is characterized to have the ability of regulating the growth rate of mycoplasma bovis and maintaining colonial morphology.

This invention relates to the field of gene technology, and more particularly to the preparation of a zebrafish model lacking Trmt61a enzyme activity, for the purpose of preparing a stable genetic model. trmt61a D181A -P2A-EGFP Knock-in zebrafish strains. This invention utilizes CRISPR / Cas9 technology to... trmt61a d181a trmt61a d181a The mutation site was specifically introduced into the zebrafish genome, resulting in the loss of Trmt61a catalytic activity and tRNA m 1 A zebrafish model with significantly reduced tRNA modification levels. This zebrafish model fills a research gap and provides a basis for tRNA m... 1 A provides a usable in vivo research model for studying the biological developmental regulatory mechanisms related to epigenetic modifications and elucidating the pathological mechanisms of diseases (such as malignant tumors and hematopoietic dysfunction), which can be directly used to explore the core role of this epigenetic modification in development and disease.

The invention belongs to the technical field of genes, discloses a method for detecting and mining associated genes and regulatory pathways by using double-layer optimization, solves the black box problem in a machine learning prediction process and provides biological mechanism explanation. The previous research mostly utilizes multi-omics data to predict phenotypes, but a machine learning mode is difficult to really evaluate the action size and action approach of genes, and lacks interpretability at the same time. According to the RADAR, a parameter is given to each omics of each gene for double-layer optimization solution, phenotypes can be explained to the maximum extent, and meanwhile the action degree of each omics can be evaluated. In addition, RADAR can process input data of any number of omics, and a program can automatically identify the number of the omics and calculate the effect size of each omics. RADAR can accept omics input of different gene numbers, and the actual data condition is better met.

This invention belongs to the field of gene technology and discloses a method for efficient expression of exogenous proteins based on the NC_048601.1 site within the CHO cell genome. The site within the CHO cell genome used for stable protein expression is located within the range of bases 16027000-16033000 of NC_048601.1 in the CHO cell genome, and its nucleotide sequence is shown in SEQ ID NO: 1. This invention introduces different protein genes into a fixed location within the CHO cell genome and achieves stable expression.

The invention discloses a dsRNA fragment capable of improving wheat amylopectin content and a synthesis method and application thereof, and relates to the field of wheat starch content regulation, pET28a is used as an initial skeleton, a sequence containing a reverse T7 promoter, multiple cloning sites and ISA1 or ISA2 gene fragment is inserted, a recombinant vector is constructed through restriction enzyme digestion connection of restriction enzymes SalI and EcoRI, and the recombinant vector is used as a recombinant vector for improving wheat amylopectin content. And transforming the recombinant expression vector into an escherichia coli HT115 competent cell, and synthesizing the dsRNA fragment after induced expression. According to the invention, a non-transgenic transient silencing technology is adopted, and target gene expression is inhibited in a targeted manner, so that the problems of long period, low efficiency, dependence on complex gene cloning and vector construction processes and potential off-target effect and ecological risk existing in the traditional gene technology are avoided.

The invention discloses a construction method and application of an hMRGPRX3 humanized mouse model and an atopic dermatitis model, and relates to the technical field of disease model construction. The whole gene sequence of the human MRGPRX3 or a vector containing the gene sequence of the hMRGPRX3 is transferred into mouse genome DNA (Deoxyribonucleic Acid) by utilizing a transgenic technology, and a functional human hMRGPRX3 receptor can be simulated and expressed in a mouse model more truly by transferring upstream and downstream regulatory sequences of the hMRGPRX3 gene. The pruritus signal transduction mediated by the receptor in human dorsal root ganglion sensory neurons can be accurately simulated, and signal deviation caused by species difference of a traditional mouse model is overcome.

The application belongs to the technical field of genes, and discloses a method for efficiently expressing exogenous proteins based on a NC_048604-1 site in a CHO cell genome. The site for stably expressing proteins in the CHO cell genome is located in a 94142000-94148000 base range of the NC_048604-1 in the CHO cell genome, and the nucleotide sequence is shown as SEQ ID NO:1. In the fixed position in the CHO cell genome, different protein genes are introduced and stably expressed.

The application belongs to the technical field of genes, and discloses a method for efficiently expressing exogenous proteins based on a Dlg3 gene site in a CHO cell genome. The Dlg3 gene site for stably expressing proteins in the CHO cell genome is located in the range of 69498000-69504000 bases of the CHO cell genome NC_048604.1, and the nucleotide sequence is shown as SEQ ID NO:1. In the fixed position of the CHO cell genome, different protein genes are introduced and stably expressed.

This invention relates to the field of gene technology, specifically disclosing a method for enhancing the secretion of secondary metabolites based on the regulation of quorum sensing signal molecules. This method activates the expression of bacterial secondary metabolite synthesis gene clusters through bacterial-fungal interaction, comprising the following steps: (a) co-culturing bacteria and fungi in contact or non-contact mode; (b) during co-culturing, the fungi secrete the quorum sensing signal molecule farnesol; (c) the bacteria sense farnesol through a two-component system, activating the expression of secondary metabolite synthesis genes. This invention effectively overcomes the gene silencing problem caused by metabolic load or lack of regulation in natural strains by activating the originally trace expression of secondary metabolite synthesis gene clusters in bacteria using the fungal-derived quorum sensing signal molecule farnesol.

This invention belongs to the field of gene technology and discloses a bacterial strain B11 antagonistic to soft rot disease of Amorphophallus muelleri. Isolated from the rhizosphere soil of Amorphophallus muelleri, it was identified as the golden subspecies of *Pseudomonas aeruginosa*. This strain effectively inhibits the growth of the soft rot pathogen Pcc, reducing the incidence and disease index of soft rot in Amorphophallus muelleri. It also possesses the ability to dissolve organophosphates and produce siderophores, and exhibits broad-spectrum antagonistic activity against various fungal diseases of Amorphophallus muelleri, including white mold, leaf spot, and dry rot. Whole-genome analysis shows that the B11 genome is 6.77 Mb in size, with a GC content of 62.81%, encoding 5,964 genes, including gene clusters related to antibacterial activity such as phenazine, nitropyrrolizin, and siderophores. This strain has good development and application potential in the control of soft rot in Amorphophallus muelleri.

The invention discloses two GST family genes linked with herbicide resistance and application of the two GST family genes, and belongs to the technical field of herbicide resistance genes. The invention discloses CDS sequences of GLGST4 and GSGST5 of Galium aparine for the first time, and the CDS sequences are respectively shown as SEQ ID NO.1 and SEQ ID NO.4; gLGST4 and GSGST5 genes are expressed in Escherichia coli for the first time, so that transgenic Escherichia coli can obtain drug resistance to florasulam, and two novel florasulam drug-resistant GST family genes derived from Galium aparine are provided; the provided GLGST4 and GSGST5 genes can be used for cultivation of ALS inhibitor herbicide-resistant crop varieties and construction, optimization and transformation of herbicide degradation engineering bacteria, and two marker genes are provided for research of an ALS inhibitor herbicide-resistant mechanism of cleavers, resistance detection of cleavers, and determination of medication time and dosage.

This invention relates to the field of rice transgenic technology, and particularly to rice genes. OsGLO2 Application in regulating rice plant height. This invention utilizes genes... OsGLO2 Overexpression in rice ZH11 significantly increased plant height and promoted rice growth. OsGLO2 The gene-editing vector at the gene-editing site was transferred into rice ZH11 to knock out the gene. Phenotypic analysis revealed that the plant height of the transgenic rice was significantly reduced. These results indicate that... OsGLO2 Genes positively regulate rice development, thereby significantly increasing rice plant height and promoting rice growth. Therefore, by regulating... OsGLO2 OsGLO2 OsGLO2 OsGLO2 OsGLO2 OsGLO2 OsG The expression level of genes in rice plants can effectively regulate rice plant height, which has great application prospects in the scientific research of molecular breeding for the cultivation of new rice varieties.

The invention relates to the technical field of biology, in particular to recombinant human ferritin, a nano-drug prepared from the recombinant human ferritin and a preparation method and application of the nano-drug. The recombinant human ferritin is obtained by taking the human heavy-chain ferritin as a template through gene technologies such as mutation and the like and an escherichia coli cloning and expression technology. The recombinant human ferritin has good water solubility and stability, the preparation method is simple, rapid, green and environment-friendly, the prepared drug carrier can efficiently embed and load insoluble small molecules under mild reaction conditions, the ferritin nano-carrier is upgraded and modified, and the application prospect is wide. The application of insoluble micromolecules in the fields of food and medicine can be expanded, and a foundation is laid for storage, in-vivo intake and delivery of the insoluble micromolecules.

The invention belongs to the technical field of genes, and discloses a method for efficiently expressing a foreign protein based on a Dlg3 gene locus in a CHO cell genome. The Dlg3 gene locus for stably expressing the protein in the CHO cell genome is located in the 69498000 -69504000 basic group range of the CHO cell genome NC048604.1, and the nucleotide sequence of the Dlg3 gene locus is as shown in SEQ ID NO: 1. According to the invention, different protein genes are introduced at fixed positions in a CHO cell genome, and stable expression is carried out.

The invention discloses application of a small heat shock protein AtHsp18.5 to improvement of drought resistance and heavy metal resistance of crops, and belongs to the field of molecular biology and biotechnology. The small heat shock protein AtHsp18.5 is coded by a gene of which the nucleotide sequence is shown as SEQ ID NO. 1. An expression vector is constructed by virtue of a genetic engineering means, and an AtHsp18.5 transgenic arabidopsis plant is obtained by virtue of a transgenic technology. Through comparative analysis of growth phenotypes and physiological and biochemical indexes of transgenic plants and wild plants, it is proved that the tolerance of the plants to drought and heavy metal stress can be remarkably improved by transferring the AtHsp18.5 gene. The invention provides a new gene resource and technical scheme for cultivating drought-resistant and heavy metal stress-resistant crop varieties by utilizing a genetic engineering means.

This invention provides a horse chestnut tiglyl-CoA ligase gene. AcCCL3 This invention relates to the AcCCL3 protein and its applications, belonging to the field of gene technology. For the first time, this invention has identified and verified the key enzyme gene responsible for the biosynthesis of tigrazol-CoA in horse chestnut. AcCCL3 This invention provides a complete analysis of its biosynthetic pathway, filling a knowledge gap in this field. It can be based on... AcCCL3 This gene, through synthetic biology strategies and an engineered system, has achieved the synthesis of tiglyl-CoA, completely eliminating reliance on traditional, complex, and demanding chemical synthesis routes. This significantly reduces production costs from the source, enabling large-scale production and successfully solving the core challenge of large-scale, stable preparation and supply of tiglyl-CoA. It also breaks through the key precursor constraints that have hindered the biosynthetic research, drug development, and industrial production of a series of high-value aescin compounds, represented by aescin A.

The application discloses application of miR396-x in regulating anthocyanin content of purple bud tea and belongs to the field of botany and gene technology. In the application, miR396-x can be used as a target point inhibitor to regulate expression of a methylase gene CsMET1 of the purple bud tea, control a methylation rate of a CsSOT gene and / or a CsF3'H1 gene of the purple bud tea, and then the anthocyanin content of the purple bud tea can be regulated, thereby providing a new direction for subsequent breeding of new tea varieties.

The invention discloses applications of a tobacco NtWRKY6 gene, a coding protein, a vector and a host cell. It is found for the first time that NtWRKY6 promotes synthesis and accumulation of tobacco polyphenols through NtPAL and NtWRKY41. A genetic material of which the expression level and the polyphenol content of related genes synthesized by polyphenols are remarkably increased due to over expression of the NtWRKY6 gene is obtained by utilizing a transgenic technology. A genetic material with the polyphenol content remarkably reduced due to NtWRKY6 function deletion is obtained by using a gene editing technology. And a new way is provided for regulating and controlling beneficial metabolites of tobacco and obtaining a new variety.

This invention belongs to the fields of fruit and vegetable science and gene technology, and discloses the application and vector of the grape VvNAC17 gene in regulating postharvest grape berry abscission. The gene sequence of the VvNAC17 gene is shown in SEQ ID NO.1, and the amino acid sequence of its encoded protein is shown in SEQ ID NO.2. The VvNAC17 silencing technology of this invention provides precise regulation at the gene molecular level, reducing the uncertainties of external environment and storage and transportation conditions, significantly reducing berry abscission rate, and improving the marketability and economic benefits of grapes during storage and transportation. By constructing a VvNAC17-RNAi vector, this invention verifies for the first time the key positive regulatory role of the VvNAC17 gene in postharvest berry abscission. The gene silencing technology does not rely on complex storage and transportation conditions and avoids the potential impact of chemical agents on fruit quality and the environment, providing an innovative solution for modern grape storage and transportation technology.

The invention relates to an application of a transporter and a gene thereof in improving tolerance of filamentous fungi to ionic liquid, and belongs to the technical field of genes. The invention aims to solve the problems that the stability of filamentous fungi in ionic liquid is poor at present, so that a fungus and ionic liquid synergistic technology is difficult to effectively apply and the like. The invention provides a transporter for improving tolerance of fungi to ionic liquid and a gene of the transporter. The nucleotide sequence of the transporter gene is shown as SEQ ID NO.1; the amino acid sequence of the transporter is as shown in SEQ ID NO. 2. After gene overexpression of the transporter provided by the invention, tolerance of filamentous fungus aspergillus terreus to ionic liquid is improved, including improvement of growth ability of a strain in the ionic liquid and improvement of activity of cellulase and hemicellulase of the strain in the ionic liquid.

The application belongs to the field of agricultural biological gene technology, and relates to a wheat powdery mildew resistance gene PmDR696 The application provides a closely linked molecular marker, a detection primer and an application. PmDR696 The molecular marker can be applied to detection, identification and auxiliary identification of a wheat powdery mildew resistance gene. PmDR696 The primer can be used for detecting whether a wheat breeding material carries the wheat powdery mildew resistance gene before the wheat powdery mildew is not developed at a wheat seedling stage. PmDR696 The marker is not only rapid, accurate, cost-saving, efficient and fast in breeding.

The invention discloses a fusion protein and application thereof in preparation of a medicine for intervening in a hepatic fibrosis process, and relates to the field of preparation of biological products. The invention designs and constructs a novel fusion protein which has targeted binding capacity and efficient thrombolysis capacity. Besides, according to the invention, a gene for coding the fusion protein and a polypeptide gene capable of specifically neutralizing plasminogen activator inhibitor 1 (PAI-1) are creatively connected through a P2A self-cleavage peptide and constructed in a single expression vector, so that co-expression of two functional proteins is realized. The co-expression system can directly and efficiently activate plasminogen through fusion protein in a hepatic fibrosis pathological microenvironment on one hand, and remove endogenous inhibition through PAI-1 polypeptide binding inhibitor on the other hand, so that degradation of fibrous protein and extracellular matrix is doubly and efficiently promoted; and a new active molecule and gene technology strategy is provided for the intervention of the hepatic fibrosis related pathological process.

The application relates to the technical field of genetic engineering, and particularly relates to application of a corn ZmMTI-9 gene in reducing cadmium accumulation in rice grains. Through early test, it is found that the corn ZmMTI-9 gene is involved in responding to the process of cadmium stress, the application carries out function verification on the gene by cloning and analyzing the corn ZmMTI-9 gene and combining with heterologous overexpression of the gene in rice through transgenic technology. The results show that the cadmium tolerance of the ZmMTI-9 overexpression plant is significantly enhanced, and the cadmium content in the grains is significantly reduced. The application provides a feasible technical scheme for rice grain low-cadmium accumulation breeding and production.