Brew yeast and application of brew yeast to manufacturing alcohol through fermentation

A technology for Saccharomyces cerevisiae and ethanol, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low ethanol yield and high production cost of fermented ethanol, achieve high economic value, improve low fermentation yield, and improve tolerance. Effect

Inactive Publication Date: 2014-05-28
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of low ethanol production in the current fermentation ethanol production, no strains with high ethanol production, and high production cost of ethanol production by fermentation, the present invention provides a strai

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Screening method for Saccharomyces cerevisiae SC-X234

[0032] The starting strain, Saccharomyces cerevisiae SC-100, was purchased from Angel Yeast Co., Ltd. Angel yeast dry powder for easy labeling, and the laboratory named it SC-100.

[0033] Screening Saccharomyces cerevisiae SC-X234 with SC-100 as the starting strain, the specific steps are as follows:

[0034] a) Preparation of single spore suspension: make spore suspension from Saccharomyces cerevisiae at a concentration of 1×10 6 individual / mL.

[0035] b) Mutagenesis of the spt15 gene mediated by low-energy ion beams: Take 100 μL of spore suspension and 50 μL of the plasmid containing spt15, evenly spread it on a sterile plate, dry it with sterile wind, and use 20Kev energy to 40×10 14 ions / cm 2 Nitrogen ion mutagenesis was carried out under the dose. After ion implantation, elution was carried out with 1mL TE solution in a sterile environment. The eluted bacterial solution was incubated at 37°C for 1...

Embodiment 2

[0049] This example illustrates the biological morphology and genetic stability of the mutant strain Saccharomyces cerevisiae SC-X234

[0050] Morphology and physiochemical characteristics of Saccharomyces cerevisiae SC-X234 of the present invention: the colony is milky white, facultative anaerobic, the colony size is 2-3mm, circular colony, the growth temperature is 35-40°C, the optimum pH is 5.0-5.5, Methylene blue staining: Live bacteria are colorless and dead bacteria are blue.

[0051] Carry out subculture experiment with the culture medium and culture condition among the embodiment 1, the result is shown in Table 2,

[0052] Table 2 Genetic stability of Saccharomyces cerevisiae SC-X234

[0053] Number of passages Alcohol production (v / v) 1 15.25 2 15 3 15 4 15.25 5 14.75 6 15.25

[0054] From the analysis of genetic stability test results, it can be known that the mutant strain Saccharomyces cerevisiae SC-X234 has stable al...

Embodiment 3

[0056] This example shows that Saccharomyces cerevisiae SC-X234 uses corn saccharification liquid to ferment alcohol

[0057] The culture medium formula described in the present embodiment is as follows:

[0058] Plate medium: glucose 2%, peptone 2%, agar 2%, yeast extract 2%, the rest is water, natural pH.

[0059] Incline medium: glucose 2%, peptone 2%, agar 2%, yeast extract 2%, the rest is water, natural pH.

[0060] Seed medium: 5% glucose, 0.5% yeast extract, 0.01% magnesium sulfate, 0.2% calcium chloride, 0.2% ammonium sulfate, 0.1% diammonium hydrogen phosphate, pH 5.5, and glucose needs to be dissolved.

[0061] Fermentation medium: corn saccharification liquid 32%, yeast extract 0.1%, calcium chloride 0.01%, ammonium sulfate 1%, diammonium hydrogen phosphate 0.5%, magnesium sulfate 0.01% pH5.5, in which glucose needs to be separated, corn saccharification liquid It is the product of corn treated with liquefaction enzyme and glucoamylase.

[0062] Saccharomyces cer...

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Abstract

The invention discloses a strain of brew yeast and application of the brew yeast to manufacturing alcohol through fermentation. The brew yeast, (Saccharomyces cerevisiae) SC-X234, is collected on January 6th, 2014 at China Center for Type Culture Collection (CCTCC), with the collection number of CCTCC M2014005; the feeble train N<+> infusion mediate spt15 gene technology is adopted, SC-100 is used as initiation strain; primary screening is conducted by utilizing a TTC slab and an alcohol slab; the obtained strain is subjected to fermentation and secondary screening to gradually increase the tolerance of the yeast in alcohol; the manufacturing strain SC-X234 with the yield of 15.75% can be finally obtained; the strain can greatly increase the fermentation productivity; the sugar to alcohol conversion ratio is high; the alcohol yield in a 5 L fermentation tank can reach 126 g/L, which is 87.5% higher than that of the initiation strain; the alcohol has a very high economic value as a fuel alcohol.

Description

technical field [0001] The invention belongs to the technical field of microbial fermentation, and in particular relates to a strain of Saccharomyces cerevisiae and its application in producing ethanol by fermentation. Background technique [0002] Fossil energy is the main body of today's energy structure, but it is a very valuable non-renewable resource with limited reserves. Taking my country as an example, although my country is a big oil producer, it is also a big oil consumer and a big shortage of oil resources. In 2008, my country produced 190 million tons of crude oil, consumed 365 million tons of oil, and imported 175 million tons of crude oil, accounting for 48% of total consumption. By the end of 2006, my country's cumulative proven recoverable oil reserves were 2.043 billion tons. According to this calculation, the existing oil can only meet the needs of several decades, and the contradiction between oil supply and demand is very prominent and will continue to in...

Claims

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Application Information

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IPC IPC(8): C12N1/19C12P7/06C12R1/865
CPCY02E50/10
Inventor 虞龙陈晓双李玉燕刘媛
Owner NANJING UNIV OF TECH
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