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Microbial agent for preventing and treating cumquat huanglongbing and preparation method thereof

A microbial inoculum, the technology of kumquat huanglong, applied in the field of prevention and control of kumquat, can solve the problems of no insecticide, unable to cut off the transmission medium of Huanglongbing, and achieve long storage period, high number of effective live bacteria and fast reproduction. Effect

Inactive Publication Date: 2017-01-25
FOSHAN YANHUI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The microbial composite bacteria include Paenibacillus Kliben and Bacillus subtilis, the biological deposit number of the Paenibacillus Klibben is CGMCC No.7996, and the microbial composite bacteria can specifically be microbial composite bacterial liquid or microorganism Compound bacterial agent can inhibit pathogenic bacteria, but has no insecticidal function, and cannot cut off the transmission medium of Huanglongbing, so as to prevent and control citrus Huanglongbing

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A microbial agent for preventing and treating huanglongbing of kumquat. The raw material ratio of the microbial agent is as follows: 20 parts of Streptomyces fermented liquid, 30 parts fermented liquid of Streptomyces kavier, 20 parts fermented liquid of Saccharomyces pyrolyticum, 20 parts of Bacillus Nissler fermented liquid, 20 parts of Trichoderma trichoderma fermented liquid;

[0049] In order to achieve the above object, a preparation method of a microbial inoculant for preventing and treating huanglongbing of kumquat, comprises the following steps:

[0050] Step 1: Preparation of Streptomyces fermented broth

[0051] Take out the preservation tube of Streptomyces spp., use Gaoshi No. 1 solid medium for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and streak inoculate it into an eggplant bottle containing 150 ml of Gaoshi No. 1 solid medium, and cultivate it in an incubator at 30°C for 7 days. After the bacterial lawn is overgrown...

Embodiment 2

[0076] Detection of the prevention and control effect of the microbial complex bacteria of the present invention on kumquat huanglongbing, the test was carried out on March 1, 2015

[0077] As of March 1, 2016, it was carried out in a fruit industry base in Xinhui District, Jiangmen City, Guangdong Province. The incidence of Huanglongbing in the experimental area was relatively serious, and the incidence rate basically reached 65%. It was carried out by root irrigation and foliar spraying. Divided into two groups of treatments, test group: 100 trees, root irrigation l00ml / plant, foliage spraying 2L / mu, all diluted 100 times; control group: 100 trees, 20% Haoniandong EC Budweiser) 1500-2000 times, beta-cypermethrin 800-1000 times; medicinal 99% mineral oil (Luying) 150-200 times + 48% chlorpyrifos 1500 times; use at least once a month;

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Abstract

The invention provides a microbial agent for preventing and treating cumquat huanglongbing and a preparation method thereof, and particularly provides a microbial agent for preventing huanglongbing of cumquat, citrus shantianju and citrus and a preparation method thereof. The microbial agent is formed by fermenting and then mixing bacteria like Gulake streptomyces, Kawuer streptomyces, bacillus psychrosaccharolyticus, nissl bacillus and tufted trichoderma proportionally. Procreation and propagation of gram negative bacteria are inhibited by means of root irrigation and foliage spray, bacteria are used to treat bacteria, and harmful pathogenic bacteria in soil are killed, so that tree root systems are more developed, tree cell tubes are smooth, yellow leaves of fruit trees are turned green, and healthy new treetops are enabled to grow; the microbial agent generates insecticidal antibiotics like flavensomycin and inhibits or damages development process of eubactericera and eggs of other disease-transmitting insect (like citrus aphid), so that adult rate is reduced, orchard pest occurrence quantity is reduced, transmission media of huanglongbing are cut off, and effects on preventing and controlling citrus huanglongbing are realized.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a microbial agent for preventing and controlling huanglongbing of kumquat, sweet orange and citrus and a preparation method thereof. Background technique [0002] Citrus (including kumquat, tangerine, and common citrus) is the most productive fruit in the world, and has been cultivated in my country for more than 4,000 years. In 2009, my country's citrus cultivation area and output surpassed Brazil and the United States, ranking first in the world. However, while the citrus industry is developing rapidly, Huanglongbing has become the most serious and devastating disease in citrus production. As early as the middle of the 18th century, there were related reports in India. [0003] Reinking reported for the first time in South my country in the 1920s. For more than half a century, citrus huanglongbing has seriously restricted the development of citrus industries in G...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/14A01N63/02A01N63/04A01P1/00C12R1/07C12R1/465C12R1/885
CPCA01N63/10A01N63/30C12N1/14C12N1/20A01N2300/00
Inventor 蒋常德胡艳晖
Owner FOSHAN YANHUI BIOTECH CO LTD
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