Microbial agent for preventing and treating cumquat huanglongbing and preparation method thereof
A microbial inoculum, the technology of kumquat huanglong, applied in the field of prevention and control of kumquat, can solve the problems of no insecticide, unable to cut off the transmission medium of Huanglongbing, and achieve long storage period, high number of effective live bacteria and fast reproduction. Effect
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Embodiment 1
[0048] A microbial agent for preventing and treating huanglongbing of kumquat. The raw material ratio of the microbial agent is as follows: 20 parts of Streptomyces fermented liquid, 30 parts fermented liquid of Streptomyces kavier, 20 parts fermented liquid of Saccharomyces pyrolyticum, 20 parts of Bacillus Nissler fermented liquid, 20 parts of Trichoderma trichoderma fermented liquid;
[0049] In order to achieve the above object, a preparation method of a microbial inoculant for preventing and treating huanglongbing of kumquat, comprises the following steps:
[0050] Step 1: Preparation of Streptomyces fermented broth
[0051] Take out the preservation tube of Streptomyces spp., use Gaoshi No. 1 solid medium for recovery, and culture at 30°C for 7 days. Pick a single colony under the plate and streak inoculate it into an eggplant bottle containing 150 ml of Gaoshi No. 1 solid medium, and cultivate it in an incubator at 30°C for 7 days. After the bacterial lawn is overgrown...
Embodiment 2
[0076] Detection of the prevention and control effect of the microbial complex bacteria of the present invention on kumquat huanglongbing, the test was carried out on March 1, 2015
[0077] As of March 1, 2016, it was carried out in a fruit industry base in Xinhui District, Jiangmen City, Guangdong Province. The incidence of Huanglongbing in the experimental area was relatively serious, and the incidence rate basically reached 65%. It was carried out by root irrigation and foliar spraying. Divided into two groups of treatments, test group: 100 trees, root irrigation l00ml / plant, foliage spraying 2L / mu, all diluted 100 times; control group: 100 trees, 20% Haoniandong EC Budweiser) 1500-2000 times, beta-cypermethrin 800-1000 times; medicinal 99% mineral oil (Luying) 150-200 times + 48% chlorpyrifos 1500 times; use at least once a month;
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