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Cotton GhMATE1 gene and application thereof to improvement of cotton brown fiber color

A brown fiber and gene technology, applied in the field of plant expression of binary vectors and improving the color of cotton brown fiber, can solve the problems of low clothing content, limited application of brown cotton, poor pigment stability, etc.

Inactive Publication Date: 2014-02-26
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Compared with white cotton, colored cotton varieties still have the following outstanding problems: low fiber specific strength, short fiber length, low clothing content, poor pigment stability, and single color, which limits the application of brown cotton in the textile industry

Method used

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  • Cotton GhMATE1 gene and application thereof to improvement of cotton brown fiber color
  • Cotton GhMATE1 gene and application thereof to improvement of cotton brown fiber color
  • Cotton GhMATE1 gene and application thereof to improvement of cotton brown fiber color

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Experimental program
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Effect test

Embodiment Construction

[0030] 1. Materials and methods

[0031] 1.1 Plant material and growth conditions

[0032] Upland cotton brown fiber cotton Zhecai No. 2, white fiber cotton Zhemian 11, diploid brown fiber cotton Cixi purple cotton and white fiber cotton Yuyao middle cotton were all produced by the Economic Crops Research Office of the Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences. Provided and live-seeded in early May 2011 in the experimental field of Hangzhou Branch of our hospital. On the 9th day (after flowering, DPA, Day Post Anthesis), 12d, 15d, 18d, 21d and 24d after cotton flowering, the developing cotton fiber tissue and seed coat tissue were selected for relevant research.

[0033] 1.2 Experimental method

[0034] 1.2.1 Extraction of cotton genomic DNA and cotton fiber and seed coat tissue total RNA

[0035] Cotton genomic DNA was extracted using the CTAB method (Li et al., 2001). The method provided by the polyphenol and polysacc...

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Abstract

The invention relates to an improved cotton fiber color gene technology, and particularly relates to a cotton gene and a plant expression binary vector constructed by the gene and application to improvement of cotton brown fiber color. A nucleotide sequence of the cotton GhMATE1 gene is shown in SEQ ID: NO.1. An amino acid sequence of a protein coded by the gene is shown in SEQ ID: NO.2. On the basis of an early-cloned cotton GhTT12a gene (Chinese patent application number: 201310380270.5, and data of application: 2013-8-27), by using a bioinformatics analysis and electronic cloning method, a new gene containing an MATE (multidrug and toxic compound extrusion) conserved domain database is cloned, but a gene structure and a shearing mode of the cotton GhMATE1 gene are completely different from those of the GhTT12a gene, and the cotton GhMATE1 gene has 78 percent of sequence homology with a nucleotide sequence of an arabidopsis thaliana testa brown pigment synthetic TT12 (Transparent Testa) gene.

Description

technical field [0001] The invention relates to a gene technology for improving cotton fiber color, in particular to a cotton gene and its constructed plant expression binary vector and its application in improving the color of cotton brown fiber. Background technique [0002] Natural colored cotton is a special type of cotton whose fiber has natural color. Because of its unique natural color, it does not need bleaching and dyeing in the process of weaving and processing. It is non-polluting to humans and the environment and is comfortable to wear. It is especially suitable for making underwear The real "ecological" and "environmentally friendly" cotton has attracted widespread attention from domestic and foreign cotton breeders and textile and clothing industries, and is known as the most potential ecological textile in the international market in the 21st century. Although the research and development of colored cotton in China started late, it developed rapidly. From 199...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/29C12N15/84C12N1/21C07K14/415A01H5/00
Inventor 李付振祝水金邱新棉吴学龙王美兴
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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