Big-data-based fusion gene data detection method, computer program, terminal

A fusion gene and data detection technology, applied in the field of genes, can solve the problems of low authenticity of fusion gene data detection data, slow detection speed, and wrong judgment.

Inactive Publication Date: 2018-12-18
TAISHAN MEDICAL UNIV
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Problems solved by technology

[0007] In the existing technology, fusion gene detection is cumbersome. Facing a huge comparative gene database, the detection speed is extremely slow and time-consuming when comparing gene sequences, which affects work efficiency and wastes manpower;
[0008] In the prior art, because the compar

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  • Big-data-based fusion gene data detection method, computer program, terminal
  • Big-data-based fusion gene data detection method, computer program, terminal
  • Big-data-based fusion gene data detection method, computer program, terminal

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Embodiment Construction

[0079] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0080] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0081] Such as figure 1 As shown, the big data-based fusion gene data detection method provided by the embodiment of the present invention includes:

[0082] S101: Compose known breakpoints of fusion genes, N sequences before the breakpoints, and M sequences behind the breakpoints to form seeds, and a collection of seeds forms a fusion gene database;

[0083] S102: using a kit to extract the RNA of the gene to be detected; using a reverse transcription kit to p...

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Abstract

The invention belongs to the field of gene technology and discloses a fusion gene data detection method based on big data, a computer program and a terminal. The known fusion gene breakpoint, N sequences in front of the breakpoint and M sequences behind the breakpoint are combined to form a seed, and the seeds are assembled to form a fusion gene database. The RNA of the gene to be detected is extracted by a kit; cDNA of the gene to be detected is obtained by reverse transcription of the extracted RNA by using a reverse transcription kit. The fusion gene is amplified by polymerase chain reaction using cDNA as template. Gene information to be detect is obtained by detect a gene fragment to be detected by a gene detection technology. The invention can effectively improve the gene sequence alignment efficiency and the user experience by comparing the sequences to be aligned according to the judgment result.

Description

technical field [0001] The invention belongs to the field of gene technology, and in particular relates to a method for detecting fusion gene data based on big data, a computer program, and a terminal. Background technique [0002] A gene (genetic element) is the entire nucleotide sequence required to produce a polypeptide chain or functional RNA. Genes support the basic structure and performance of life. It stores all the information about race, blood type, conception, growth, apoptosis and other processes of life. The interdependence of environment and heredity plays an important role in the reproduction of life, cell division and protein synthesis and other important physiological processes. All life phenomena such as birth, growth, decline, disease, old age, and death of organisms are related to genes. It is also an internal factor that determines the health of life. Therefore, genes have dual attributes: materiality (way of existence) and informationality (fundament...

Claims

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Application Information

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IPC IPC(8): G06F19/24G06F19/22G06F19/28
Inventor 赵宝昌张吉梅
Owner TAISHAN MEDICAL UNIV
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