48results about How to "High amplification efficiency" patented technology

The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses, a detection method and application, and particularly belongs to the technical field of porcine pathogen detection products. The invention provides a primer probe group for simultaneously detecting American strains and European strains of porcine reproductive and respiratory syndrome viruses. The primer probe group comprises universal forward primers PRRSV1-F and PRRSV2-F for detection, reverse primers PRRSV1-R and PRRSV2-R for detection and a universal probe PRRSV-P. A target gene detected by the primer probe group is a 3'non-coding region gene (3 'UTR) of a porcine reproductive and respiratory syndrome virus genome, and the primer probe group can realize efficient detection of American strains and European strains of the porcine reproductive and respiratory syndrome virus, and is simple and convenient in detection, high in sensitivity, good in specificity, high in speed, low in false positive and capable of realizing high-throughput detection.

The invention discloses a kit and a detection method for detecting an internally-running sheep virus epidemic strain, the kit comprises a crRNA sequence and an RT-RPA primer, the detection method comprises an RT-RPA reaction system and a CRISPR / cas12a reaction system, RT-RPA nucleic acid amplification and CRISPR / cas12a nucleic acid detection are combined, a two-step detection method is adopted, firstly, RT-RPA nucleic acid amplification is carried out, secondly, RT-RPA nucleic acid amplification is carried out, thirdly, RT-RPA nucleic acid amplification is carried out, secondly, CRISPR / cas12a nucleic acid detection is carried out, and thirdly, RT-RPA nucleic acid detection is carried out. Then, the RT-RPA nucleic acid product is added into a CRISPR / cas12a nucleic acid detection system, and the Chinese epidemic strain of the internally-running sheep virus can be detected. The detection method is simple, efficient, low in dependence on instruments and equipment, good in sensitivity, specificity and stability and good in social application prospect.

The invention discloses a universal identification primer, kit and method for hippocampus. The universal identification primer for the hippocampus comprises HipF: 5 '-GGCCATACCCAAAAATG-3' and HipR: 5 '-TTTAGCTTATTAAAGTGTCTGTTTTGCA-3'. The universal identification primer for the hippocampus can be used for identifying 10 types of hippocampus, and the amplification efficiency of the universal identification primer is higher than that of the identification primer in the prior art, so that conditions are provided for developing an efficient, accurate and practical hippocampus variety identification technical method and effectively popularizing and improving the hippocampus variety identification technical capability.

The invention discloses a primer and probe combination for detecting a bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR (Polymerase Chain Reaction). The invention further discloses a method for detecting the bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR, the bulbus fritillariae cirrhosae specific primers and probes designed based on six bulbus fritillariae cirrhosae-based original plant conserved gene sequences and the 18sRNA universal reference gene primers and probes are adopted, a sample to be tested and a standard reference substance are amplified at the same time in a PCR reaction system, and the specific primers and probes are used for detecting the specific genes of the bulbus fritillariae cirrhosae medicinal material based on Taqman fluorescent probe double-marking linear correction quantitative PCR. The method comprises the following steps: qualitatively confirming that a test sample contains the bulbus fritillariae cirrhosae component according to amplification results of a bulbus fritillariae cirrhosae specific primer and a probe, then taking a standard reference substance as a template to obtain two standard curves of a bulbus fritillariae cirrhosae specific gene and a 18sRNA gene, and finally quantitatively analyzing the bulbus fritillariae cirrhosae content in the test sample by adopting a double-marking linear correction method. The method can be used for efficiently, specifically and accurately detecting the ingredients and content of the bulbus fritillariae cirrhosae through PCR.

The invention belongs to the technical field of biological and genetic engineering, and particularly relates to a nucleic acid amplification efficiency optimization control method and a nucleic acid quantitative detection system. According to the nucleic acid amplification efficiency optimization control method disclosed by the invention, the nucleic acid amplification efficiency is optimized by combining control modes such as temperature and fluorescence real-time regulation and control, and the nucleic acid amplification efficiency is optimized by monitoring the real-time state of the nucleic acid amplification reaction and dynamically and adaptively adjusting the temperature condition of the nucleic acid amplification reaction; the nucleic acid amplification efficiency is improved, the quantitative detection repeatability is optimized, the nucleic acid amplification efficiency is improved, the amplification consistency is optimized, and the quantitative detection repeatability of the nucleic acid is effectively improved.

The invention relates to the field of biotechnology primer design, in particular to a target species specific primer design system, method and device based on information entropy. The system comprises a data acquisition module, a data screening module and a data optimization module, the data screening module is used for acquiring short sequence fragment data with identity and coverage greater than or equal to 80%, and the data optimization module is used for processing the short sequence fragment data with identity and coverage greater than or equal to 80%. According to the method, short sequence fragments covering 90% of genomes are screened out, the entropy value of each basic group in the short sequence fragments is obtained, quantitative scoring is conducted on the short sequence fragments according to the entropy values and coverage degree information, the short sequence fragments with the top scores are screened out, the basic groups are replaced with the most conservative basic groups, and the optimized short sequence fragments are obtained. And designing primers based on the short sequence fragments. According to the system, entropy analysis is introduced in the primer design process, and the design quality and application efficiency of primers are remarkably improved.

The invention relates to a picosecond laser with high beam quality, comprising: a pumping source for providing pumping energy to an amplification crystal; the laser oscillator is used for providing signal light with high light beam quality for the amplification crystal; the amplification crystal is used for amplifying the signal light power; the pump light shaping system comprises a first lens group and a third plano-convex lens, the first lens group is arranged between the pump source and the amplification crystal and formed by combining a first plano-convex lens and a second plano-convex lens, the third plano-convex lens and the first lens group are arranged at intervals, and the pump light is subjected to quasi-collimation processing through the first lens group; third plano-convex lenses which are arranged at intervals are used for focusing, so that the Rayleigh length of the pump light after focusing completely falls into the amplification crystal; the signal light shaping system comprises a fourth plano-convex lens arranged on the upstream of the light path of the amplification crystal and a second lens group formed by combining a fifth plano-convex lens and a first plano-concave lens, and the signal light is focused through the fourth plano-convex lens. After beam expansion and collimation are carried out through the second lens group, the Rayleigh length of the signal light completely falls into the amplification crystal, the pump light and the signal light are in a uniform distribution state in the amplification crystal, and the amplification crystal generates uniform heat distribution to eliminate the thermal lens effect. The generation probability of a thermally induced birefringence effect and a thermally induced teratosis effect is reduced, and the amplification efficiency of the light beam and the quality of the output light beam are greatly improved.

The invention discloses a method for detecting the copy number of mRNA (messenger ribonucleic acid) of a novel crown RSV (respiratory syncytial virus) combined vaccine. A primer probe combination adopted by the detection method consists of primer pairs as shown in SEQ ID NO.2 and SEQ ID NO.3 and a probe as shown in SEQ ID NO.4, the 5'end of the probe is marked with a fluorescence reporter group, and the 3 'end of the probe is marked with a fluorescence quenching group. According to the detection method, correlation coefficients of equations between the Cq value and the template copy number are both larger than 0.99, amplification efficiency is both 90%-110%, and specificity and accuracy are good; the method can be used for quality control processes such as release detection in the combined vaccine development process and toxicological experiments, and has important significance and value for research and development and quality control of combined vaccines.

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

This invention discloses a primer set for amplifying a novel HCV subtype 6xp and a primer set for detecting drug resistance mutations. The primer set for amplifying the novel HCV subtype 6xp includes nested PCR primers for amplifying the full length of the novel HCV subtype 6xp, while the primer set for detecting drug resistance mutations is a set of primers targeting drug resistance mutation sites in the three functional regions of NS3, NS5A, and NS5B. The primer set provided by this invention has high specificity and high amplification efficiency, enabling specific amplification of the entire genome of the novel HCV subtype 6xp and accurate detection of drug resistance mutation sites in the three functional regions. It is suitable for clinical diagnosis, antiviral treatment guidance, epidemiological surveys, and viral evolution research of this subtype, and has significant clinical application value and scientific research significance.

The present invention discloses a primer, crRNA and application thereof for broad-spectrum detection of mycoplasma based on LAMP-CRISPR / Cas12a, and relates to the technical field of mycoplasma detection. The primer and crRNA combination provided by the present invention includes 2 outer primers, 5 inner primers, 4 loop primers and crRNA. Based on this, the present invention also provides a LAMP-CRISPR / Cas12a system and product for broad-spectrum detection of mycoplasma, as well as a method for detecting mycoplasma that is not for the purpose of disease diagnosis and treatment. The primer and crRNA combination provided by the present invention and the corresponding detection method can simultaneously meet the requirements of specificity, specificity, detection coverage (broad-spectrum detection) and sensitivity stipulated by various countries and the European Pharmacopoeia; the operation is simple, the cost is low, the time consumption is short, the detection efficiency is high, the dependence on expensive instruments is low, and the instant detection of mycoplasma can be realized.

The invention belongs to the field of molecular biology of forestry insects, and particularly relates to a fluorescent quantitative reference gene of different gender adults of yellow shield elephant, as well as a primer and application of the fluorescent quantitative reference gene. According to the method, 8 candidate genes commonly used as reference genes are referenced and selected according to transcriptome sequencing data of the hedychium aureum, gene sequences of the 8 reference genes are identified, the stability of the candidate reference genes is evaluated through five algorithms, and the fluorescent quantitative reference genes suitable for the hedychium aureum adults with different genders are obtained. And a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer of the candidate reference gene is designed. The detection primer provided by the invention is strong in specificity and high in amplification efficiency, fills up the current situation that no reference gene exists under adults with different genders of the hedychium aureum, provides a reliable analysis basis for expression quantification of related functional genes under the adults with different genders of the hedychium aureum, and has a good application prospect. A necessary earlier-stage foundation is laid for analysis of subsequent molecular mechanisms and mining of key functional genes, and repeatability, stability and reliability of functional gene research can be improved at the same time.

The application belongs to the technical field of biological detection, and particularly relates to a nucleic acid detection primer probe composition for typhus and an integrated microfluidic chip kit. The primer probe composition is used for detecting typhus, and has good sensitivity and accuracy, high amplification efficiency, and simple operation, rapidness and time saving. The application integrates nucleic acid extraction, purification, amplification and detection by using the microfluidic mode on the integrated chip, solves the problem of rapid detection of pathogens, realizes a POCT detection scheme of "sample in-result out", and directly adds the equipment after sampling, purifies and amplifies, and the result is obtained within 60 minutes.

The invention discloses a reagent for detecting African swine fever virus gene type II and application of the reagent. The reagent comprises a primer group, the primer group comprises a forward primer with a sequence as shown in SEQ ID NO: 2 and a reverse primer with a sequence as shown in SEQ ID NO: 3. Compared with other similar kits, the kit for detecting the African swine fever gene type II, provided by the invention, has the advantages that the sensitivity can be remarkably improved through the design of the primers, the specificity is high, the sensitivity is high, the amplification efficiency is high, the detection time is short and the like; according to the kit provided by the invention, a user does not need to carry out reagent split charging, the extracted sample nucleic acid is directly added into the pre-split freeze-dried reagent to be detected in a fluorescent PCR instrument, and the operation is simple and rapid.

The invention provides an SLAMP primer group and a kit for detecting mycobacterium tuberculosis, and relates to the technical field of biological detection. The invention specifically discloses an SLAMP primer group and a kit for detecting mycobacterium tuberculosis. The SLAMP primer group is a primer group aiming at the amplification of a conserved region fragment of mycobacterium tuberculosis, and is a primer pair MTB-SF / MTB-SR; wherein the nucleotide sequence of the MTB-SF is as shown in SEQ ID NO. 1, and the nucleotide sequence of the MTB-SR is as shown in SEQ ID NO. 2. According to the method or the visual kit provided by the invention, rapid and accurate detection of the mycobacterium tuberculosis can be completed without expensive instruments or complicated operation, so that the method or the visual kit is suitable for on-site rapid detection in a scene with a low professional degree.

This invention belongs to the field of molecular biology technology, specifically relating to a primer-probe set for RT-RAA detection of goose astrovirus and its application. The primer-probe set includes an upstream primer F1, a downstream primer R2, and a probe P. This primer-probe set can simultaneously detect two genotypes of goose astrovirus, GoAstV-I and GoAstV-II; it exhibits high specificity and amplification efficiency, aiming to provide a rapid and convenient technical means for the early diagnosis, on-site monitoring, and epidemic prevention and control of goose astrovirus. Example results show that using GoAstV-I, GoAstV-II, GPV, GoCV, DTMUV, and NDV nucleic acids as templates for RT-RAA-LFD detection, positive bands were observed for GoAstV-I and GoAstV-II, while all other pathogens were negative, with no cross-reaction, indicating that the specificity of the primer-probe set is 100%.

The invention discloses a varicella-zoster virus whole genome capturing method, a primer group and a kit. The primer combination for amplifying the whole genome sequence of the varicella-zoster virus disclosed by the invention can effectively amplify the whole genome sequence of the varicella-zoster virus, is high in coverage rate, and is beneficial to further research on pathogenic mechanisms, molecular epidemiology, etiology and the like of the varicella-zoster virus.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for promoting proliferation of immature bone marrow-derived macrophages and polarization of the immature bone marrow-derived macrophages to M1 through stress application culture and application of the method. The in-vitro stress application culture method of the macrophages from the immature bone marrow has the characteristics of high stability, high amplification efficiency, polarization controllability, high purity, flexibility in application, low production cost and the like; the problems that a traditional cell factor induction method is low in amplification efficiency, poor in polarization controllability, insufficient in purity and the like are solved, the purity of the obtained M1 type macrophage reaches 90% or above, stability is high, and cost is low. The method can be applied to preparation of macrophage drugs for immunoregulation, anti-fibrosis, metabolism regulation, anti-tumor CAR-M and the like, tissue regeneration biological materials, anti-inflammatory drugs, in-vitro diagnosis products and the like, dosage forms are diversified, and a standardized cell preparation scheme is provided for the related biological medicine field.

The present invention discloses an OPA energy conversion efficiency improvement device, which includes two nonlinear optical OPA crystals and a 1 / 2 wave plate, wherein the 1 / 2 wave plate is placed between the nonlinear optical OPA crystal 1 and the nonlinear optical OPA crystal 2, and is placed perpendicular to the laser propagation direction, and the distance between the 1 / 2 wave plate and the front and rear nonlinear optical OPA crystals 1 and the nonlinear optical OPA crystal 2 is equal. The present invention avoids the signal light energy loss caused by the reverse frequency sum process, and greatly improves the amplification efficiency of the OPA device.

The application belongs to the technical field of nucleic acid amplification, and discloses a Phi29 DNA polymerase mutant with improved amplification efficiency and application thereof. Compared with wild-type phi29 DNA polymerase, the Phi29 DNA polymerase mutant provided by the application comprises at least one of the following mutation sites: M102Q, P558L, T534R, M102R, T534P, Y224K, G217E, T140P, H284Y, V19P. The Phi29 DNA polymerase mutant has excellent amplification efficiency, and the band brightness obtained by amplification under the same conditions is significantly higher than that of the wild type, and the amplification efficiency is as high as more than twice that of the wild type. The application has good application prospect in the field of nucleic acid amplification and sequencing.

The invention relates to the field of molecular biology, in particular to a primer group, an MYH3 gene promoter region DNA methylation level analysis method and application. The primer group comprises a first primer pair and a second primer pair, the upstream sequence of the first primer pair is as shown in SEQ ID NO. 1; the downstream sequence of the first primer pair is as shown in SEQ ID NO. 2; the upstream sequence of the second primer pair is as shown in SEQ ID NO.3; and the downstream sequence of the second primer pair is as shown in SEQ ID NO.4. The method for analyzing the methylation level of the DNA in the MYH3 gene promoter region relates to PCR reaction conditions for obtaining qualified target DNA fragments for MSP sequencing, the primer group and the PCR reaction conditions are combined to have the advantage of high amplification efficiency, the qualified DNA fragments are obtained through amplification, the DNA fragments are provided for analyzing the methylation level of the DNA in the MYH3 gene promoter region, and the methylation level analysis efficiency and accuracy are improved.

The present application relates to the technical field of plantago asiatica L. genetic engineering, and specifically provides a plantago asiatica L. real-time fluorescent quantitative PCR internal reference gene and application thereof. The tender leaves, old leaves, stems, flowers and roots of plantago asiatica L. are selected as experimental materials, candidate internal reference genes are screened through plantago asiatica L. transcriptome, then the gene expression stability is analyzed through real-time fluorescent quantitative PCR analysis, melting curve analysis, amplification efficiency analysis and various software (geNorm, NormFinder, BestKeeper, Delta Ct and RefFinder) analysis, and the internal reference genes suitable for gene quantification of different tissue parts of plantago asiatica L., i.e. eIF and Cycl genes, are screened, and the sequences are shown as SEQ ID NO. 1 and SEQ ID NO. 2. The internal reference genes with stable expression are obtained, are suitable for analyzing the expression amount of genes in different parts of plantago asiatica L., make up for the current situation that plantago asiatica L. lacks internal reference genes, and provide a basis for gene expression amount and function analysis in plantago asiatica L.

The invention relates to a nucleic acid amplification and chromatographic detection kit based on ureaplasma urealyticum in a totally-enclosed card box and application of the kit, the kit is a kit based on an RPA isothermal amplification chromatography technology, a freeze-drying ball reagent, a liquid reagent and a nucleic acid detection chromatographic test strip are arranged in the kit, the amplification mode is RPA isothermal amplification, and the detection sensitivity is high. The detection mode is a chromatographic test strip, the 5'end of a probe of the ureaplasma urealyticum RPA is marked with digoxin, and the 5 'end of a downstream primer is marked with biotin. The 5'end of the RPA probe of the internal standard substance bacillus is marked with FAM, and the 5 'end of the downstream primer is marked with biotin. Two indexes of UU and reference genes can be simultaneously amplified in the same tube through an RPA isothermal amplification method, an amplification product is double-stranded DNA with molecular markers at two ends, compared with fluorescent PCR, the kit integrates nucleic acid extraction, amplification and detection, the nucleic acid extraction, amplification and detection are all carried out in a card box in a sealed mode, a sample adding step is not needed, operation is simple, pollution can be effectively prevented, and the kit is suitable for large-scale popularization and application. And automation of the whole process is realized.

The invention discloses a primer group for amplifying a serotype dengue type II virus genome. The primer group comprises a primer pair 1 to a primer pair 16, and the nucleotide sequences of the 16 primer pairs are sequentially shown as SEQ ID NO: 1 to SEQ ID NO: 32. The invention also discloses a targeted amplification kit which contains the primer group and is used for rapidly amplifying the serotype dengue type II virus. The invention also discloses application of the primer group and the kit in preparation of a product for amplifying a serotype dengue type II virus genome. The invention also discloses a method for rapidly obtaining a serotype dengue type II virus whole genome based on targeted amplification. The targeted amplification primer group consists of 16 pairs of primers, can cover and amplify all current epidemic serotype dengue II virus strains, and is high in amplification efficiency and less in cross interference; meanwhile, genomes obtained by a downstream sequencing method and an analysis method constructed by the amplicon are high in sequencing precision and large in sequencing flux.

The invention discloses a low-defect-density compound semiconductor film and a preparation method and application thereof, and the method comprises the steps: alternately growing a compound semiconductor layer and a defect blocking layer on a substrate, and enabling the defect blocking layer to be provided with a groove-shaped structure, and to be used for limiting defect propagation and guiding the transverse growth of the compound semiconductor layer; gradually expanding the low-defect area through multiple alternate growth until a complete low-defect-density compound semiconductor film is formed; depositing a two-dimensional material layer on the terminal layer grown on the compound semiconductor film for subsequent stripping of the low-defect-density compound semiconductor film; and transferring the separated film to a new substrate, and repeating the growing and stripping steps to realize industrial batch production. The defect barrier layer with the groove-shaped structure is introduced to the surface of the thin film, the deposition and stripping technology of the two-dimensional material layer is combined, defect propagation can be effectively limited, the crystal quality of the thin film is improved, lossless separation and repeated transfer of the thin film are achieved, and the method is suitable for batch industrial production.

The present invention discloses a method for monitoring the germplasm of chuatsi (Siniperca chuatsi) using microsatellite genetic markers, including extraction and detection of genomic DNA, search and analysis of microsatellite sequences, screening and optimization of polymorphic microsatellite primers, detection of polymorphic microsatellites, and monitoring of different geographical chuatsi populations and their genetic traits; the present invention screens a large number of ESTs generated by chuatsi transcriptome sequencing for microsatellite sites and develops and verifies polymorphic microsatellite markers, and develops 15 EST-SSR polymorphic microsatellite markers, which can monitor different geographical populations of chuatsi and their genetic traits. The method can make up for the limitations of the current chuatsi germplasm detection, improved variety breeding and germplasm improvement research, such as the relatively vague genetic background and the small number of molecular markers, and provide a basis for the chuatsi germplasm detection, improved variety breeding and germplasm improvement, thereby promoting the progress of chuatsi molecular marker germplasm detection, assisted genetic breeding and genomics research.

The application discloses a CRISPR-Cas12a detection primer group for Francisella tularensis and application thereof. The application provides a CRISPR-Cas12a system for detecting Francisella tularensis, and also provides RAA amplification primers for specifically amplifying the target sequence of the Francisella tularensis in the first aspect; and a probe as a fluorescent reporter molecule is also provided. The application obtains a Francisella tularensis specific gene sequence based on whole genome alignment of Francisella tularensis and its close species, further utilizes the specific gene sequence to design RAA amplification primers and crRNA sequences to detect Francisella tularensis, and develops a related kit. The application can specifically detect Francisella tularensis and distinguish it from other close species, and has the characteristics of rapidness, simplicity and accuracy.

This invention relates to a gene diagnostic biomarker and kit for diagnosing refractory gastroesophageal reflux disease (GERD), belonging to the field of biomedical technology. The invention provides the application of a biomarker, NRF1 and / or NRF2, in the preparation of a diagnostic reagent for refractory GERD; a diagnostic reagent for diagnosing GERD, comprising reagents for detecting NRF1 and / or NRF2 expression; a diagnostic kit for diagnosing GERD, comprising reagents for detecting NRF1 and / or NRF2 expression; and the application of the reagent for detecting NRF1 and / or NRF2 expression in the preparation of a diagnostic reagent for refractory GERD, comprising primers for detecting NRF1 and / or NRF2 expression. This invention provides a reliable tool for the rapid and accurate diagnosis of refractory GERD.

The application discloses a rice product core No.1 primer group for detecting rice quality functional genes, a primer pool, a kit and application thereof, and belongs to the technical field of biology. The rice product core No.1 primer group comprises a first primer pair to a 40th primer pair, each primer pair comprises a forward primer and a reverse primer, and the forward primer of the first primer pair, the reverse primer of the first primer pair to the forward primer of the 40th primer pair and the reverse primer of the 40th primer pair are sequentially shown as SEQ ID NO:1 to SEQ ID NO:80 in a sequence table. The rice product core No.1 primer group, the primer pool and the kit provided by the application can be used for detecting rice quality functional genes, and provide a method for rapidly, accurately and low-cost detecting variation information of appearance quality genes and taste quality genes of rice for rice genetic and breeding workers.

This invention belongs to the field of radio frequency integrated circuit technology and discloses a stacked broadband radio frequency amplifier and its wireless transceiver circuit. The stacked broadband radio frequency amplifier includes an input impedance matching network, a first stacked amplifier circuit, a second stacked amplifier circuit, and an output impedance matching network. The input terminal of the input impedance matching network serves as the input terminal of the stacked broadband radio frequency amplifier and is connected to the radio frequency signal input terminal. The output terminal of the input impedance matching network outputs the radio frequency signal to the first stacked amplifier circuit. After receiving the radio frequency signal, the input terminal of the first stacked amplifier circuit outputs a first-stage amplified radio frequency signal. After receiving the first-stage amplified radio frequency signal, the first stacked amplifier circuit further amplifies and outputs a second-stage amplified radio frequency signal. The input terminal of the output impedance matching network receives the second-stage amplified radio frequency signal and outputs the amplified radio frequency signal. The amplifier structure of this invention is simple and has excellent high power output, low phase distortion, and wide temperature stability.