4results about How to "High amplification efficiency" patented technology

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

This invention discloses a primer set for amplifying a novel HCV subtype 6xp and a primer set for detecting drug resistance mutations. The primer set for amplifying the novel HCV subtype 6xp includes nested PCR primers for amplifying the full length of the novel HCV subtype 6xp, while the primer set for detecting drug resistance mutations is a set of primers targeting drug resistance mutation sites in the three functional regions of NS3, NS5A, and NS5B. The primer set provided by this invention has high specificity and high amplification efficiency, enabling specific amplification of the entire genome of the novel HCV subtype 6xp and accurate detection of drug resistance mutation sites in the three functional regions. It is suitable for clinical diagnosis, antiviral treatment guidance, epidemiological surveys, and viral evolution research of this subtype, and has significant clinical application value and scientific research significance.

This invention relates to a gene diagnostic biomarker and kit for diagnosing refractory gastroesophageal reflux disease (GERD), belonging to the field of biomedical technology. The invention provides the application of a biomarker, NRF1 and / or NRF2, in the preparation of a diagnostic reagent for refractory GERD; a diagnostic reagent for diagnosing GERD, comprising reagents for detecting NRF1 and / or NRF2 expression; a diagnostic kit for diagnosing GERD, comprising reagents for detecting NRF1 and / or NRF2 expression; and the application of the reagent for detecting NRF1 and / or NRF2 expression in the preparation of a diagnostic reagent for refractory GERD, comprising primers for detecting NRF1 and / or NRF2 expression. This invention provides a reliable tool for the rapid and accurate diagnosis of refractory GERD.

The invention belongs to the technical field of biology, and discloses a primer group and application thereof, and a kit for detecting RPA-CRISPR / Cas12a of ALV-F. According to the technical scheme, the primer group for specifically amplifying an ALV-F sequence is characterized by comprising an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.2; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO. 2; based on a conserved region of an avian leukosis virus (ALV) F subgroup genome, a set of RPA primer group and crRNA with high specificity and high amplification efficiency are autonomously designed and verified. According to the design, the genetic diversity of target viruses is effectively covered, high specificity of detection on ALV-F subgroups is ensured, and the risk of cross reaction with other poultry pathogens is reduced to the maximum extent.