16results about How to "High amplification efficiency" patented technology

The invention discloses a universal identification primer, kit and method for hippocampus. The universal identification primer for the hippocampus comprises HipF: 5 '-GGCCATACCCAAAAATG-3' and HipR: 5 '-TTTAGCTTATTAAAGTGTCTGTTTTGCA-3'. The universal identification primer for the hippocampus can be used for identifying 10 types of hippocampus, and the amplification efficiency of the universal identification primer is higher than that of the identification primer in the prior art, so that conditions are provided for developing an efficient, accurate and practical hippocampus variety identification technical method and effectively popularizing and improving the hippocampus variety identification technical capability.

This invention belongs to the field of hybrid identification technology of *Fraxinus pubescens*, specifically relating to an InDel marker and its application for identifying the authenticity of hybrids between *Fraxinus pubescens* and *Astragalus membranaceus*. The InDel marker described in this invention is located at positions 187 to 240 of the sequence shown in SEQ ID NO:1, and the sequence of the InDel marker is shown in SEQ ID NO:2. Furthermore, based on this InDel marker, this invention has obtained a set of primers with stable polymorphism, high amplification efficiency, and clear bands, establishing a technical system that can identify the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment. This provides an efficient and low-cost molecular tool for the breeding of new varieties among *Fraxinus pubescens* species, the protection of variety rights, and market supervision, and has significant application value.

This invention discloses a primer set for amplifying a novel HCV subtype 6xp and a primer set for detecting drug resistance mutations. The primer set for amplifying the novel HCV subtype 6xp includes nested PCR primers for amplifying the full length of the novel HCV subtype 6xp, while the primer set for detecting drug resistance mutations is a set of primers targeting drug resistance mutation sites in the three functional regions of NS3, NS5A, and NS5B. The primer set provided by this invention has high specificity and high amplification efficiency, enabling specific amplification of the entire genome of the novel HCV subtype 6xp and accurate detection of drug resistance mutation sites in the three functional regions. It is suitable for clinical diagnosis, antiviral treatment guidance, epidemiological surveys, and viral evolution research of this subtype, and has significant clinical application value and scientific research significance.

The invention belongs to the field of molecular biology of forestry insects, and particularly relates to a fluorescent quantitative reference gene of different gender adults of yellow shield elephant, as well as a primer and application of the fluorescent quantitative reference gene. According to the method, 8 candidate genes commonly used as reference genes are referenced and selected according to transcriptome sequencing data of the hedychium aureum, gene sequences of the 8 reference genes are identified, the stability of the candidate reference genes is evaluated through five algorithms, and the fluorescent quantitative reference genes suitable for the hedychium aureum adults with different genders are obtained. And a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) primer of the candidate reference gene is designed. The detection primer provided by the invention is strong in specificity and high in amplification efficiency, fills up the current situation that no reference gene exists under adults with different genders of the hedychium aureum, provides a reliable analysis basis for expression quantification of related functional genes under the adults with different genders of the hedychium aureum, and has a good application prospect. A necessary earlier-stage foundation is laid for analysis of subsequent molecular mechanisms and mining of key functional genes, and repeatability, stability and reliability of functional gene research can be improved at the same time.

The application belongs to the technical field of biological detection, and particularly relates to a nucleic acid detection primer probe composition for typhus and an integrated microfluidic chip kit. The primer probe composition is used for detecting typhus, and has good sensitivity and accuracy, high amplification efficiency, and simple operation, rapidness and time saving. The application integrates nucleic acid extraction, purification, amplification and detection by using the microfluidic mode on the integrated chip, solves the problem of rapid detection of pathogens, realizes a POCT detection scheme of "sample in-result out", and directly adds the equipment after sampling, purifies and amplifies, and the result is obtained within 60 minutes.

The invention belongs to the technical field of biological medicines, and particularly relates to a method for promoting proliferation of immature bone marrow-derived macrophages and polarization of the immature bone marrow-derived macrophages to M1 through stress application culture and application of the method. The in-vitro stress application culture method of the macrophages from the immature bone marrow has the characteristics of high stability, high amplification efficiency, polarization controllability, high purity, flexibility in application, low production cost and the like; the problems that a traditional cell factor induction method is low in amplification efficiency, poor in polarization controllability, insufficient in purity and the like are solved, the purity of the obtained M1 type macrophage reaches 90% or above, stability is high, and cost is low. The method can be applied to preparation of macrophage drugs for immunoregulation, anti-fibrosis, metabolism regulation, anti-tumor CAR-M and the like, tissue regeneration biological materials, anti-inflammatory drugs, in-vitro diagnosis products and the like, dosage forms are diversified, and a standardized cell preparation scheme is provided for the related biological medicine field.

The present application relates to the technical field of plantago asiatica L. genetic engineering, and specifically provides a plantago asiatica L. real-time fluorescent quantitative PCR internal reference gene and application thereof. The tender leaves, old leaves, stems, flowers and roots of plantago asiatica L. are selected as experimental materials, candidate internal reference genes are screened through plantago asiatica L. transcriptome, then the gene expression stability is analyzed through real-time fluorescent quantitative PCR analysis, melting curve analysis, amplification efficiency analysis and various software (geNorm, NormFinder, BestKeeper, Delta Ct and RefFinder) analysis, and the internal reference genes suitable for gene quantification of different tissue parts of plantago asiatica L., i.e. eIF and Cycl genes, are screened, and the sequences are shown as SEQ ID NO. 1 and SEQ ID NO. 2. The internal reference genes with stable expression are obtained, are suitable for analyzing the expression amount of genes in different parts of plantago asiatica L., make up for the current situation that plantago asiatica L. lacks internal reference genes, and provide a basis for gene expression amount and function analysis in plantago asiatica L.

The invention relates to a nucleic acid amplification and chromatographic detection kit based on ureaplasma urealyticum in a totally-enclosed card box and application of the kit, the kit is a kit based on an RPA isothermal amplification chromatography technology, a freeze-drying ball reagent, a liquid reagent and a nucleic acid detection chromatographic test strip are arranged in the kit, the amplification mode is RPA isothermal amplification, and the detection sensitivity is high. The detection mode is a chromatographic test strip, the 5'end of a probe of the ureaplasma urealyticum RPA is marked with digoxin, and the 5 'end of a downstream primer is marked with biotin. The 5'end of the RPA probe of the internal standard substance bacillus is marked with FAM, and the 5 'end of the downstream primer is marked with biotin. Two indexes of UU and reference genes can be simultaneously amplified in the same tube through an RPA isothermal amplification method, an amplification product is double-stranded DNA with molecular markers at two ends, compared with fluorescent PCR, the kit integrates nucleic acid extraction, amplification and detection, the nucleic acid extraction, amplification and detection are all carried out in a card box in a sealed mode, a sample adding step is not needed, operation is simple, pollution can be effectively prevented, and the kit is suitable for large-scale popularization and application. And automation of the whole process is realized.

The application discloses a CRISPR-Cas12a detection primer group for Francisella tularensis and application thereof. The application provides a CRISPR-Cas12a system for detecting Francisella tularensis, and also provides RAA amplification primers for specifically amplifying the target sequence of the Francisella tularensis in the first aspect; and a probe as a fluorescent reporter molecule is also provided. The application obtains a Francisella tularensis specific gene sequence based on whole genome alignment of Francisella tularensis and its close species, further utilizes the specific gene sequence to design RAA amplification primers and crRNA sequences to detect Francisella tularensis, and develops a related kit. The application can specifically detect Francisella tularensis and distinguish it from other close species, and has the characteristics of rapidness, simplicity and accuracy.

This invention relates to a gene diagnostic biomarker and kit for diagnosing refractory gastroesophageal reflux disease (GERD), belonging to the field of biomedical technology. The invention provides the application of a biomarker, NRF1 and / or NRF2, in the preparation of a diagnostic reagent for refractory GERD; a diagnostic reagent for diagnosing GERD, comprising reagents for detecting NRF1 and / or NRF2 expression; a diagnostic kit for diagnosing GERD, comprising reagents for detecting NRF1 and / or NRF2 expression; and the application of the reagent for detecting NRF1 and / or NRF2 expression in the preparation of a diagnostic reagent for refractory GERD, comprising primers for detecting NRF1 and / or NRF2 expression. This invention provides a reliable tool for the rapid and accurate diagnosis of refractory GERD.

The application discloses a rice product core No.1 primer group for detecting rice quality functional genes, a primer pool, a kit and application thereof, and belongs to the technical field of biology. The rice product core No.1 primer group comprises a first primer pair to a 40th primer pair, each primer pair comprises a forward primer and a reverse primer, and the forward primer of the first primer pair, the reverse primer of the first primer pair to the forward primer of the 40th primer pair and the reverse primer of the 40th primer pair are sequentially shown as SEQ ID NO:1 to SEQ ID NO:80 in a sequence table. The rice product core No.1 primer group, the primer pool and the kit provided by the application can be used for detecting rice quality functional genes, and provide a method for rapidly, accurately and low-cost detecting variation information of appearance quality genes and taste quality genes of rice for rice genetic and breeding workers.

This invention belongs to the field of radio frequency integrated circuit technology and discloses a stacked broadband radio frequency amplifier and its wireless transceiver circuit. The stacked broadband radio frequency amplifier includes an input impedance matching network, a first stacked amplifier circuit, a second stacked amplifier circuit, and an output impedance matching network. The input terminal of the input impedance matching network serves as the input terminal of the stacked broadband radio frequency amplifier and is connected to the radio frequency signal input terminal. The output terminal of the input impedance matching network outputs the radio frequency signal to the first stacked amplifier circuit. After receiving the radio frequency signal, the input terminal of the first stacked amplifier circuit outputs a first-stage amplified radio frequency signal. After receiving the first-stage amplified radio frequency signal, the first stacked amplifier circuit further amplifies and outputs a second-stage amplified radio frequency signal. The input terminal of the output impedance matching network receives the second-stage amplified radio frequency signal and outputs the amplified radio frequency signal. The amplifier structure of this invention is simple and has excellent high power output, low phase distortion, and wide temperature stability.

The invention relates to a ferrocene metal organic framework / carbon-based material composite membrane, a polymerase chain reaction photo-thermal module and application thereof. The ferrocene metal organic framework / carbon-based material composite film is prepared by performing suction filtration and film forming on dispersion liquid containing a carbon-based material and a ferrocene metal organic framework. And the polymerase chain reaction photo-thermal module can realize the control of temperature rise and / or temperature reduction based on the regulation and control of the irradiation power of the light source. Compared with the prior art, the ferrocene metal organic framework / carbon-based material composite membrane and the polymerase chain reaction photo-thermal module can realize efficient photo-thermal conversion under the irradiation of a light source, have the characteristics of excellent heating and cooling rate and low power consumption, and provide a new solution for rapid pathogen detection.

The application belongs to the technical field of identification of Fraxinus velutina hybrid, and particularly relates to an InDel marker for identifying the authenticity of Fraxinus velutina hybrid and application thereof. The InDel marker is located at positions 63-150 of the sequence shown in SEQ ID NO:2, and the sequence of the InDel marker is shown in SEQ ID NO:1. Meanwhile, based on the InDel marker, the application obtains a group of primers with stable polymorphism, high amplification efficiency and clear bands, and establishes a technical system for identifying the authenticity of hybrid offspring at the seedling stage without relying on expensive equipment, thereby providing an efficient and low-cost molecular tool for Fraxinus velutina new variety breeding, variety right protection and market supervision, and having good application value.

The invention belongs to the technical field of biology, and discloses a primer group and application thereof, and a kit for detecting RPA-CRISPR / Cas12a of ALV-F. According to the technical scheme, the primer group for specifically amplifying an ALV-F sequence is characterized by comprising an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is as shown in SEQ ID NO.1, and the nucleotide sequence of the downstream primer is as shown in SEQ ID NO.2; the nucleotide sequence of the downstream primer is as shown in SEQ ID NO. 2; based on a conserved region of an avian leukosis virus (ALV) F subgroup genome, a set of RPA primer group and crRNA with high specificity and high amplification efficiency are autonomously designed and verified. According to the design, the genetic diversity of target viruses is effectively covered, high specificity of detection on ALV-F subgroups is ensured, and the risk of cross reaction with other poultry pathogens is reduced to the maximum extent.

The invention discloses a kit and method for detecting the copy number of mtDNA in drosophila melanogaster, the kit comprises a drosophila melanogaster genome DNA extraction reagent and a drosophila melanogaster mtDNA amplification primer, high-purity genome DNA can be efficiently extracted from trace drosophila melanogaster tissue, the ratio of A260 / A280 to A260 / A230 is close to 2.0, and the downstream molecular experiment requirement is met. Meanwhile, a pair of qPCR primers for specifically amplifying a drosophila melanogaster mtDNA ND5 gene region is designed and screened out, and experiments prove that the amplification efficiency of the primer pair is as high as 98.4%, the linear relation is excellent (R is equal to 0.9995), the sensitivity is high, no primer dimer and non-specific amplification exist, and the performance is remarkably superior to that of other contrast primers. Therefore, the kit can be used for detecting the copy number of the mtDNA in the drosophila melanogaster, and an efficient, sensitive and reliable special tool is provided for researching the mitochondrial function of the drosophila melanogaster and related diseases.