71results about How to "Reduce chlorophyll content" patented technology

The invention discloses a stress tolerance associated protein TaSAP1 of plants as well as a coding gene and an application thereof. Experiments prove that the survival rate of a T3 generation TaSAP1 transformed homozygous plant obtained by using the recombinant expression vector pCH3-GFP-TaSAP1 of DNA (deoxyribonucleic acid) molecules shown by nucleotide sequences at sites from 26th to 532nd in a sequence table 2 for converting the arabidopsis in drought tolerance experiment is 54.2%-66.7%; the survival rates of a wild type plant and an empty vector-transformed plant are respectively 14.6% and 20.8%; in a salt tolerance experiment, compared with a wild type contrast plant and an empty vector-transformed plant contrast plant, the TaSAP1 transformed plant has the advantages that the reduction of chlorophyll content in the TaSAP1 transformed plant is slow in comparison than that before the salt tolerance stress. The TaSAP1 protein and the coding gene thereof provided by the invention have important significance in improvement of plant adverse resistance, provide a basis for artificial control expression of stress tolerance associated gene and play an important role in breeding plants with high stress tolerance such as strong drought tolerance and strong salt tolerance.

The invention relates to a modified atmosphere freshness keeping production technology of wild Marsilea quadrifolia. The modified atmosphere freshness keeping production technology is characterized in that the technology comprises the following steps: picking a raw material, and pre-cooling; 2, choosing the raw material; 3, immersion: immersing in an immersion liquid composed of a material and water according to a volume ratio of 1:9-11, wherein the material comprises a 1% salt solution; 4, cleaning: removing dusts and silts; 5, dehydration: dehydrating by using a centrifuge; 6, disinfection: disinfecting through using an ultraviolet ray sterilizer; 7, modified atmosphere packaging: carrying out seal packaging of the Marsilea quadrifolia through using a freshness keeping film, and filling a mixed gas composed of O2, CO2 and N2 according to a certain ratio; and 8, refrigerating. The modified atmosphere freshness keeping production technology allows the Marsilea quadrifolia to be packaged by the mixed gas composed of O2, CO2 and N2 according to a certain ratio and to be stored at a low temperature, so the change of the nutritional components of the modified atmosphere Marsilea quadrifolia is reduced, and the original freshness, color and flavor of the Marsilea quadrifolia are furthest kept to realize the prolongation of the storage life and the regulation of the market surplus and deficiency.

The invention discloses an ailanthus altissima bark algistat and a preparation method and an application thereof, and belongs to the field of water boy pollution control. The algistat is an absolute ethyl alcohol crude extract of ailanthus altissima bark. Compared with the prior art, the defects that an existing chemical method causes secondary pollution and is high in cost, unlikely to be controlled, likely to damage the ecological environment and the like are overcome, and the limitation that the algistat is high in technical cost and complicated in operation is solved by utilizing water plant extraction. The algistat is extracted from the woody plant ailanthus altissima bark, and the preparation method is simple, convenient to use and high in operability and is suitable for large-scale popularization. The prepared algistat has high inhibition efficiency for microcystis aeruginosa, and can become effective on the second day; the inhibition ratio reaches more than 90%, and the water body can be effectively prevented from breaking out water bloom for a long period of time.

The invention provides a plant antifreezing solution which comprises the following components in parts by weight: 2 to 5 parts of paraffin, 5 to 8 parts of vegetable oil, 4 to 8 parts of polybutylene, 3 to 9 parts of potassium fulvic, 5 to 9 parts of benzoic acid, 2 to 6 parts of tea polyphenol, 2 to 5 parts of citric acid and 2 to 5 parts of a cooking liquor of cassia twig, sappanwood, ginger and ampelopsis japonica. The ion content of Cu, Fe, Mn, Zn and B in the antifreezing solution is greater than or equal to 100 g/L. The plant antifreezing solution is simple in composition and convenient to use, the freeze injury damage on trees can be greatly reduced, the antifreezing effect is good, and the plant antifreezing solution activates biological enzyme, prevents the generation of ice nucleus bacteria, breaks ice forming activity of freezing protein, and increases heat. The ice point value of plant cells is reduced, the flow of cellular plasm is promoted, the cell viability is improved by 4 to 8 times, the water holding and drought resisting abilities of the plant are improved, freeze injury danger is reduced, functions of reducing and recovering are played on middle and light freeze injury plants, effects on serious damage are in vain, and the ice point value of the plant is reduced by 5 to 8 degrees.

The invention relates to a smallanthus sonchifolius buccal tablet and a preparation method thereof, belonging to the field of pharmaceutical technology. The smallanthus sonchifolius buccal tablet takes concentrated dry powder of smallanthus sonchifolius juice as main drug component as well as fermented smallanthus sonchifolius leaves, chrysanthemum and liquorice extract as adjuvant components, and the mixture of the main drug component and the adjuvant components is added with auxiliary material powdered glucose, xylitol, microcrystalline cellulose, starch and magnesium stearate which are needed in the preparation of the buccal tablet; finally, the mixed component is shaped by being pressed into tablets by a tablet machine. The smallanthus sonchifolius buccal tablet has the characteristics of diminishing inflammation, inhibiting bacteria, refreshing, losing weight, being good in mouthfeel and easy in absorption.

The invention belongs to the technical field of genes and especially relates to application of a gene Loc_Os01g12810. The gene Loc_Os01g12810 can be applied to rice chloroplast development regulationmechanism researches; a gene method is utilized to regulate an expression level of the gene Loc_Os01g12810 to research rice chloroplast development regulation mechanisms; a nucleotide sequence of thegene Loc_Os01g12810 is shown in SEQ ID NO:1 in a sequence table; an amino acid sequence of encoding protein of the gene Loc_Os01g12810 is shown in SEQ ID NO:2 in the sequence table. According to the application of the gene Loc_Os01g12810 disclosed by the invention, that the rice gene Loc_Os01g12810 is a gene related with rice chloroplast development is proved for the first time. The Loc_Os01g12810is a PPR gene. Cloning and biological function verification of the gene have important reference significance in rice chloroplast development molecule mechanism researches and researches on effects of PPR family gene in rice development.

The invention discloses a rice yellow-green leaf related protein as well as an encoding gene and an application of the rice yellow-green leaf related protein. The rice yellow-green leaf related protein provided by the invention is a protein with an amino acid sequence as shown in SEQ ID No.1 or is a protein which is obtained by adding, substituting, inserting or deleting one or more amino acids in the amino acid sequence as shown in SEQ ID No.1, is related to rice yellow-green leaves and is derived from SEQ ID No.1. The invention also discloses an encoding gene of the protein, and the gene has nucleotide sequences as shown in SEQ ID No.2 and 3. The yellow-green leaf control gene disclosed by the invention can also be used as an indication mark of false hybrids in a hybrid seed production process and is applied to crossbreeding improvement and breed improvement.

The invention relates to a method for improving the salt resistance of a lawn plant by filling soil through adopting a waste colloidal particle as a salt storing layer and application of the waste colloidal particle in improving the salt resistance aspect of the lawn plant Festuca arundinacea. Results indicate that the waste colloidal particle can improve the underground biomass and chlorophyll content of the plant to a certain extent; and the addition of the waste colloidal particle obviously improves the activity of superoxide dismutase (SOD) in the set NaCl concentration range. The invention not only can improve the physical characteristics of soil air permeability, soil water potential, and the like but also can improve the elasticity of a lawn substance by adopting the waste colloidal particle as a soil medium and constructing the salt storing layer, and is unmatched with other soil additives; and the waste colloidal particle improves the salt resistance of the Festuca arundinacea.

The invention provides a method for reducing water eutrophication. The method is that a submerged plant and a filter freshwater bivalve mollusk are bred in eutrophic water, wherein the submerged plant comprises one or more than two selected from Myriophyllum, Elodea, Ceratophyllum, and Hydrilla varticillata, and the filter freshwater bivalve mollusk is hyriopsis cumingii. Compared with some present floating technologies, the method of the invention not only utilizes roots of the submerged plant to absorb nitrogen and phosphor, but also utilizes leaves of the submerged plant to absorb eutrophic substances in water, so the oxygen dissolving rate of water is improved; and the method of the invention has the advantages of simple operation, low cost, no secondary pollution, and generation of certain economic benefits through combining with the aquatic product culture.

The invention provides a fresh white tea cultivation technique and belongs to the technical field of tea planting. The fresh white tea cultivation technique comprises the steps of setting up a greenhouse, managing a tea garden, cultivating before sprouting, managing sprouting, picking white tea and picking white tea for a second time. The white tea cultivated through the cultivation technique is low in chlorophyll content, and the manufactured white tea is high in content of tea polyphenol, condenced phenols and other aromatic substances and has the characteristics of being white in color, low in content of brass substances, clear in liquor color, light in color and sweet after tasting. Meanwhile, nutrition solution sprayed before sprouting can make tea grow quickly and be large in content of zinc and selenium elements and more excellent in nutrition and health-care effects.

The invention belongs to the technical field of identification and screening of low-temperature-resistant maize inbred lines, and particularly relates to an identification and screening method of a seedling-stage low-temperature-resistant maize inbred line. The identification and screening method of the low-temperature-resistant maize inbred line comprises the following steps: carrying out low-temperature stress treatment and normal-temperature treatment on maize seedlings to be detected; detecting indexes of the corn seedlings obtained by the two treatment modes; screening out a low-temperature-resistant corn selfing line according to a detection result, wherein the conditions of the low-temperature stress treatment are as follows: culturing is carried out at 6-8 DEG C for 7 days, the indexes are seedling length, chlorophyll content and proline content. According to the method, the optimal treatment condition for low-temperature identification of the maize inbred line in the seedlingstage is determined; the selfing line with strong seedling stage low temperature resistance has good seedling plant form, obvious increase of proline content and less decrease of chlorophyll content under low temperature stress, can reduce the damage of low temperature to the seedling stage growth of plants, provides a powerful guarantee for the growth and development of the seedling stage, and lays a foundation for the large-scale identification and screening of low temperature resistant corn germplasms.

The invention provides a cold-resistant regulator. Active ingredients of the cold-resistant regulator comprise magnesium sulfate, calcium chloride, chitosan, sodium dimethyl dithiocarbamate, abscisic acid, basil extracting solution and palm oil. All the components of the cold-resistant regulator have a synergistic effect, and the cold-resistant regulator has the functions of improving the photosynthetic efficiency of plants, improving the structural stability of cell membranes, maintaining the fresh weight of the plants and the like; under the effect of low-temperature stress, decrease of chlorophyll content, net photosynthetic rate, intercellular carbon dioxide concentration and the like of rubber tree leaves can be effectively slowed down, and increase of relative conductivity of phloem, malondialdehyde content and the like can be remarkably slowed down; the content of phloem soluble sugar, the maximum photochemical efficiency of leaf PSII, the actual photochemical efficiency of leaf, the content of phloem soluble protein, the content of phloem superoxide dismutase, the activity of phloem peroxidase and the like can be remarkably improved, and damage of low-temperature stress to a phloem cell membrane and a leaf photosynthetic system of a rubber tree is reduced; rubber flowing of the rubber trees is inhibited, and damage of rubber flowing to the rubber trees is reduced.

The invention provides a spraying fluid capable of preventing a trunk from freezing and bursting. The spraying fluid is prepared from, by weight, 2-5 parts of ethylene glycol, 5-8 parts of methyl ester, 4-8 parts of isobutene, 3-9 parts of potassum fulvic acid, 5-9 parts of calcium chloride, 2-6 parts of tea polyphenol, 2-5 parts of citric acid and 2-5 parts of alcohol leaching liquor of rhizoma ligustici, indigowoad leaves, fistular onion stalks and cinnamon. The content of ions Cu+Fe+Mn+Zn+B in an antifreezing agent is larger than or equal to 105 g/L. The spraying fluid capable of preventing the trunk from freezing and bursting is easy to match, convenient to use, capable of greatly reducing frost damage of trees and good in anti-freezing effect; the spraying fluid activates biological enzymes, prevents ice nucleating bacteria from generating, destroys ice-forming activity of frozen protein and increases heat. Freezing point values of plant cells are reduced, flow of cellular plasm is promoted, and the cell viability is increased by 4-8 times. Water retention and drought resistance capacities of the plants are increased, frost damage is decreased, reducing and rehabilitating effects on middle-light freezing plants are achieved, the spraying fluid is invalid for sever damage, and the freezing point values of the plants are decreased by 5-8 degrees.

The invention provides a use method of a tree anti-freezing spraying agent. The use method includes the use steps of A, diluting the tree anti-freezing spraying agent into 800-volume-fold liquid through water to be sprayed in late autumn and before plant dormancy; B, diluting the tree anti-freezing spraying agent into 600-volume-fold liquid through water to irrigate roots 3-7 days before frosting or cold waves; C, diluting the tree anti-freezing spraying agent into 1000-volume-fold liquid through water to irrigate the roots before and after plant transplanting; D, diluting the tree anti-freezing spraying agent through water by 50-80 volume folds to conduct trunk and branch brushing operation before winter. The steps are required to be conducted at the temperature between 3 DEG C and 8 DEG C. The tree anti-freezing spraying agent comprises 1-hydroxy ethylidene-1,1-diphosphonic acid, tolyl triazole sodium, polybutylene, fulvic acid potassium, benzoic acid, tea polyphenol, phosphoric acid triethanolamine, Chinese mosla herbs, ginger, chrysanthemum and burdock. The use method is simple in ratio, convenient to use, capable of greatly reducing cold injury loss of trees and good in anti-freezing effect.

The invention provides an antifreezing agent, wherein the antifreezing agent comprises the following components in parts by weight: 2-5 parts of paraffin, 5-8 parts of vegetable oil, 4-8 parts of glycerinum, 3-9 parts of acetic acid, 5-9 parts of salicylic acid, 2-6 parts of tea polyphenol, 2-5 parts of glacial acetic acid, and 2-5 parts of a cooking liquor of bamboo leaves, ochre, Chinese mosla herb and pine needles. The antifreezing agent is simple in ratio and convenient to use, greatly reduces the loss caused by the freeze injury of a tree, and has an excellent antifreezing effect. The antifreezing agent activates the biological enzyme, stops the production of ice nucleating bacteria, breaks the icing activity of frozen protein, increases the heat, reduces the freezing point of a vegetable cell, promotes the flow of the cellular plasm, and improves the cell viability by 4-8 times. The water-retaining and drought-resisting abilities of the plant are improved, the freeze injury is alleviated, the antifreezing agent has an effect of alleviating and recovering the moderate and mild freeze injury plants, has no effect on the severe injury, and reduces the freezing point of the plant by 5-8 DEG C.

The invention discloses a fresh-keeping method for prolonging the shelf life of fresh-cut asparagus, and belongs to the technical field of fruit and vegetable processing and fresh-keeping. The fresh-keeping method comprises the following steps: performing ultrasonic treatment on fresh-cut asparagus by using a composite cleaning agent, and fumigating with cinnamon essential oil. The composite cleaning agent is a mixed solution of citric acid and nisin. Different cleaning modes are combined with cinnamon essential oil fumigation for preservation of the fresh-cut asparagus, and the method can effectively inhibit growth of microorganisms, delay aging of the fresh-cut asparagus and degradation of chlorophyll, delay increase of lignin content, maintain nutritional quality and maintain good sensory quality of the fresh-cut asparagus, so that the purpose of prolonging the shelf life of the fresh-cut asparagus is achieved.

The invention discloses application of a chlorosis seedling lethality 1 (CSL1) gene to regulation of development of rice chloroplasts. According to the invention, a lethal rice mutant at an etiolatedseedling stage is isolated from a rice T-DNA mutant library; the lethal rice mutant is inserted into a seventh intron of the CSL1 gene by means of T-DNA insertion; a 109bp sequence is deleted at a T-DNA insertion position; the chlorophyll content of the CSL1 mutant decreases; single-layer or multi-layer autophagosomes appear in the mesophyll cells; starch grains in the chloroplasts increase; a thylakoid structure is disordered; and the expression quantity of chloroplast-related genes is changed. meanwhile, the invention further constructs a knockout plant (the CSL1 gene has a deletion of one base A at the site 46 of exon 5, or a deletion of one base A at the site 47 and substitution of the base A with the base T at the site 48) by means of gene knockout, and the knockout plant has the samephenotype as the CSL1 mutant, thus showing that the CSL1 gene is involved in regulation of development of the rice chloroplasts.

The invention provides a preparation method of a root system anti-freezing solution. The preparation method comprises the following steps: preparing the following materials: paraffin, calcium chloride, methanol, fulvic acid, glycerol, distilled water, mono carboxylic acid, ramulus mori, pine needle, rhizoma zingiberis and herb of diffuse dayflower; A, mixing methanol, glycerol, distilled water and mono carboxylic acid, uniformly stirring, then adding fulvic acid into the mixture, adding paraffin (liquid paraffin) into the mixture, uniformly stirring and leaving to stand; B, placing ramulus mori, pine needle, rhizoma zingiberis and herb of diffuse dayflower into a closed vessel, adding an ethanol solution with the mass percent of 75 into the closed vessel, infiltrating and soaking the active components for 12 h, after that, charging the active components into a percolator, adding ethanol with the mass percent of 75 for soaking, then starting to collect the percolating liquid, heating the collected percolating liquid with 75 percent of ethanol to reclaim ethanol so as to obtain an extract, adding the extract into calcium chloride to enable the extract to be dissolved, heating dissolved calcium chloride to be 40-50 DEG C, and sterilizing for later use; C, fully mixing the product obtained in the step A and the product obtained in the step B. The proportioning is simple, the use is convenient, frost injury loss of trees is greatly reduced and the anti-freezing effect is good.

The invention provides a Stenotrophomonas sp. strain KT48, algicidal bacterial liquid as well as a preparation method and application of the algicidal bacterial liquid. The preservation number of the Stenotrophomonas sp. strain KT48 is CGMCC NO. 22722, and the algicidal bacterial liquid is prepared from the Stenotrophomonas sp. strain KT48 with the preservation number of CGMCC NO. 22722; the Stenotrophomonas sp. strain KT48 has an algae removal effect instead of a metabolite of the Stenotrophomonas sp. strain KT48, so that the Stenotrophomonas sp. strain KT48 is more convenient for practical application; when the algicidal bacterial liquid is prepared, a culture medium is inoculated with the Stenotrophomonas sp. strain KT48, then, the culture medium is cultured in a constant-temperature oscillation box to a stable phase so as to obtain the algicidal bacterial liquid, and thus, the preparation method is simple; the Stenotrophomonas sp. strain KT48 can be used for breaking cell membranes of microcystis aeruginosa and destroying cell structures of the microcystis aeruginosa; and the algicidal bacterial liquid prepared by adopting the Stenotrophomonas sp. strain KT48 can be used for lowering the chlorophyll content of blue-green algae and effectively controlling water bloom outbreak, and has high efficiency, biological specificity, environmental friendliness and biodegradability. When the algicidal bacterial liquid is used for controlling blue-green algae bloom, the cost is low, the operation is simple, the safety is high, and secondary pollution is avoided.

The invention relates to a foliar fertilizer for promoting yellowing of flue-cured tobacco and a use method. The foliar fertilizer is prepared from the following components in parts by weight and volume: 0.2 to 0.3 kg/L of monopotassium phosphate, 0.1 to 0.2 kg/L of sodium bicarbonate, 15 to 25mg/L of abscisic acid and 10 to 20mg/L of auxin. According to the technical scheme, the foliar fertilizercan effectively reduce the chlorophyll content in the later period of flue-cured tobacco and increase the carotenoid content, and yellowing of flue-cured tobacco in the later period of field production is obvious. And the ratio of yellow smoke in baking quality is obviously improved, the ratio of green smoke, green smoke and yellow smoke in micro-strip is reduced, and the flue-cured tobacco output value and economic benefit are remarkably increased.