6results about How to "Reduce chlorophyll content" patented technology

The invention discloses an application of a GHERF114 gene in regulating and controlling salt stress tolerance and drought stress tolerance of a plant. The ID (Identity) of the gene is GHA05G2445. According to the invention, an arabidopsis thaliana homozygous line over-expressing the GHERF114 gene is obtained through a transgenic technology; through phenotypic change after salt stress treatment and drought stress treatment, leaf wilting degree, rosette leaf size and form comparison, in-vitro leaf water loss rate, leaf relative water content, chlorophyll content and the like verification, it is indicated that the tolerance of transgenic arabidopsis thaliana to salt stress and drought stress is remarkably reduced, and a TRV: GHERF114 gene silent plant is obtained through a VIGS technology; compared with a control group after salt stress and drought stress treatment, the salt tolerance and the drought resistance of silent plants are remarkably improved. Results show that the GHERF114 plays an important role in plant salt stress and drought stress response processes, has important significance in breeding and research for improving salt tolerance and drought tolerance of cotton, and can be applied to screening of stress-resistant cotton varieties through a gene knockout technology.

The invention belongs to the field of application of postharvest preservation of potatoes, and particularly relates to a method for inhibiting greening and germination of postharvest potatoes and application of the postharvest potatoes, and the method comprises the following steps: screening, warming, cleaning, disinfecting, soaking and airing the potatoes, and then carrying out shelf life test; wherein the soaking is performed by adopting a calcium chloride solution; the shelf life test is illumination or non-illumination treatment. Research finds that after the potatoes are harvested, the potatoes in the non-dormancy period are soaked for 20 minutes by adopting a 0.8 wt% calcium chloride solution, and the potatoes in the dormancy period are soaked for 5 minutes by adopting a 0.6 wt% calcium chloride solution, so that the greening inhibition and the bud inhibition of the potatoes after the harvesting can be simply and effectively realized, and important reference is provided for further exploration of a greening inhibitor and a bud inhibitor of the harvested fruits and vegetables.

The invention relates to the technical field of agricultural cultivation, in particular to a method for preventing and treating pathogenic fungus infection after continuous cropping of tomatoes by combining calcium propionate with trichoderma harzianum, and the method can enhance the disease resistance of the tomatoes after continuous cropping, improve the quality of the tomatoes and promote the growth of the tomatoes. It is found for the first time that combined use of calcium propionate and trichoderma harzianum can comprehensively prevent and treat common mixed fungus infection after tomato continuous cropping, and particularly, the composition has a comprehensive prevention and treatment effect on mixed fungus infection of Colletotrichum gloeosporioides, Colletotrichum oleifera, Fusarium graminearum and Amoerella almoka.

The application relates to the field of agricultural transgenic technology, and particularly discloses GhDBB28 application of a gene in regulating salt tolerance of upland cotton plants, the gene GhDBB28 The nucleotide sequence of the gene is shown as SEQ ID NO. 1; silencing the gene GhDBB28 increases the salt sensitivity of the upland cotton plants. The application reduces the activity of an antioxidant system in the upland cotton plants by silencing the gene GhDBB28 , so that the plants are more sensitive to salt stress, and a scientific basis is provided for constructing a transgenic upland cotton plant with new salt-sensitivity.

The present invention discloses the application of rice Ospep5 gene and its encoded small peptide in regulating plant salt tolerance. The small peptide is 34 amino acids in length and belongs to an unknown gene in rice. Its nucleotide sequence is shown in SEQ ID NO: 1. The present invention can significantly improve the salt tolerance of rice and Arabidopsis by exogenously applying Ospep5 small peptide in rice and Arabidopsis. The relevant biological material of the present invention is an expression cassette, recombinant vector, recombinant bacteria or transgenic cell line capable of expressing the Ospep5 nucleic acid molecule or containing the nucleic acid molecule. The present invention shows that knocking out the Ospep5 gene in rice using the CRISPR / Cas9 gene editing method significantly reduces the salt tolerance of rice; while the transgenic rice overexpressing Ospep5 has significantly improved salt tolerance, proving that the Ospep5 gene positively regulates the salt tolerance of rice, which is of great significance for breeding new salt-tolerant plant varieties.

The invention discloses exogenous spraying liquid for tobacco leaves and application of the exogenous spraying liquid. The exogenous spraying liquid is prepared by mixing an EBR solution, an inositol solution and a Tween 80 solution. The invention further discloses application of the exogenous spraying liquid for the tobacco leaves in the aspects of promoting the maturity of the tobacco leaves before harvesting and improving the quality of flue-cured tobaccos. According to the method, the exogenous spraying liquid formed by mixing the EBR solution, the inositol solution and the Tween 80 solution is sprayed before the tobacco leaves are harvested, the chlorophyll content can be reduced, the tobacco leaves are promoted to be mature, the appearance quality of the tobacco leaves is improved, the internal chemical components are improved, the quality and economic value of flue-cured tobaccos in the honey-sweet-flavor ecological region are remarkably improved, and the method is suitable for being popularized in the honey-sweet-flavor ecological producing region.