Tobacco leaf exogenous spraying liquid and application thereof

Abstract

The invention discloses exogenous spraying liquid for tobacco leaves and application of the exogenous spraying liquid. The exogenous spraying liquid is prepared by mixing an EBR solution, an inositol solution and a Tween 80 solution. The invention further discloses application of the exogenous spraying liquid for the tobacco leaves in the aspects of promoting the maturity of the tobacco leaves before harvesting and improving the quality of flue-cured tobaccos. According to the method, the exogenous spraying liquid formed by mixing the EBR solution, the inositol solution and the Tween 80 solution is sprayed before the tobacco leaves are harvested, the chlorophyll content can be reduced, the tobacco leaves are promoted to be mature, the appearance quality of the tobacco leaves is improved, the internal chemical components are improved, the quality and economic value of flue-cured tobaccos in the honey-sweet-flavor ecological region are remarkably improved, and the method is suitable for being popularized in the honey-sweet-flavor ecological producing region.

Description

Technical Field

[0001] The invention relates to an exogenous spraying liquid for tobacco leaves and application thereof, belonging to the technical field of tobacco leaf conditioning. Background Art

[0002] Tobacco is a widely planted cash crop in the world. It is valued worldwide for its important role in increasing national income, promoting local economic development and increasing tobacco farmers' income. It is also widely concerned and studied because its growth conditions, post-baking yield and quality are closely related to economic benefits. According to the latest tobacco regional division, most areas of Guizhou Province belong to the typical Guizhou-Guangxi mountain ecological zone - the honey sweet fragrance ecological zone. The main feature of the tobacco leaves produced is the outstanding honey sweet fragrance, mainly liquorice fragrance and honey sweet fragrance, supplemented by mellow sweet fragrance, dry fragrance, sweet fragrance, sour fragrance, caramel fragrance, baking fragrance, etc. It has moderate concentration and strength, but it has trace amounts of unpleasant miscellaneous odors, such as green miscellaneous odor, raw green odor and woody odor. In tobacco production, natural disasters or improper field management during the maturity period of tobacco, such as insufficient field light, heavy rainfall, or excessive nitrogen application, can cause tobacco leaves to turn green, leading to the resynthesis of chlorophyll in the leaves and delayed maturity. When immature tobacco leaves are harvested for baking, their high chlorophyll content degrades slowly, and the baking properties of tobacco leaves deteriorate, resulting in green leaves. After baking, the color of tobacco leaves is floating green, with serious variegation, and produces impurities, which reduces the appearance and internal quality of tobacco leaves. It is necessary to study and improve this. Summary of the invention

[0003] Based on the above, the present invention provides an exogenous spray liquid for tobacco leaves and its application, which can promote the early yellowing and maturity of tobacco leaves and improve the quality of flue-cured tobacco, so as to overcome the shortcomings of the prior art.

[0004] The technical solution of the present invention is: In a first aspect, the present invention provides an exogenous spraying liquid for tobacco leaves, wherein the exogenous spraying liquid is prepared by mixing an EBR solution, an inositol solution, and a Tween 80 solution.

[0005] Optionally, in the exogenous spray solution, EBR, inositol and Tween 80 are mixed in a volume ratio of 80-90:2-10:1-5.

[0006] Optionally, the concentration of the EBR solution is 1 µmol / L, and the preparation method thereof is as follows: EBR is first dissolved in 3 mL of anhydrous ethanol to prepare a mother solution, and then diluted with distilled water to a concentration of 1 µmol / L.

[0007] Optionally, the concentration of the inositol solution is 100 µmol / L.

[0008] Optionally, the concentration of the Tween 80 solution is 0.1 mg / L.

[0009] In a second aspect, the present invention provides the use of the exogenous spray liquid for tobacco leaves in promoting the maturity of pre-harvest tobacco leaves and improving the quality of flue-cured tobacco.

[0010] Optionally, tobacco leaves at a mature stage are selected, and an exogenous spraying liquid is sprayed on the leaf surface before harvesting.

[0011] Optionally, the tobacco leaves are tobacco leaves that are in the maturity stage but not fully mature, including upper leaves and middle leaves.

[0012] Optionally, the spraying time is 2 to 3 times of spraying the solution on the leaves within two weeks ± 3 days before the normal harvest period of the tobacco leaves, with an interval of 1 to 3 days between spraying the solution.

[0013] Optionally, the tobacco leaves are sprayed with the solution evenly on both sides of the leaves of a single tobacco plant using a sprayer in the clear and windless morning or evening until the front and back sides of each tobacco leaf are soaked and begin to drip with the solution.

[0014] The beneficial effects of the present invention are as follows: by spraying an exogenous spraying liquid mixed with an EBR solution, an inositol solution and a Tween 80 solution before tobacco leaves are harvested, the chlorophyll content can be reduced, tobacco leaves can be accelerated to mature, the appearance quality of tobacco leaves can be improved and the coordination of the internal chemical components can be improved, and the tobacco leaves are suitable for promotion in the ecological production areas of the sweet and fragrant type.

[0015] In the present invention, EBR (abscisic acid) can promote the maturity of tobacco leaves, reduce the chlorophyll content by adjusting the balance of plant hormones, and help the leaves turn yellow in advance; inositol, as a plant signal molecule, improves the overall quality of tobacco leaves by promoting cell metabolism and growth; Tween 80, as a surfactant, can enhance the permeability and uniform distribution of the spraying liquid, ensure that the effective ingredients are better absorbed by the leaves, thereby improving the spraying effect; the combination of EBR, inositol and Tween 80 not only improves the maturity, but also improves the appearance and internal quality of flue-cured tobacco by optimizing internal metabolism and chemical composition. The present invention promotes the maturity of tobacco leaves by spraying on the leaves before harvesting, and significantly improves the quality and economic value of flue-cured tobacco in the honey-sweet fragrance ecological zone. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 This is a comparison between fresh tobacco leaves and flue-cured tobacco in the middle of tobacco leaves; Figure 2 This is the result diagram of the SPAD value of fresh tobacco leaves in the middle of tobacco; Figure 3 This is the result diagram of the color difference parameters of the fresh tobacco leaves in the middle of the tobacco leaves; Figure 4 This is the result diagram of the chemical component content of flue-cured tobacco in the middle part of tobacco leaves.

[0017] Figure 5 This is a graph showing the scoring results of the appearance quality evaluation of the middle tobacco leaves.

[0018] Figure 6 This is a comparison between fresh tobacco leaves and real flue-cured tobacco leaves; Figure 7 This is the result diagram of the SPAD value of the fresh leaves of the upper tobacco leaves; Figure 8 This is the result diagram of the color difference parameters of the fresh leaves of the upper tobacco leaves; Fig. 9 This is a graph showing the scoring results of the appearance quality evaluation of the upper tobacco leaves.

[0019] In the figure, CK represents tobacco leaves sprayed with distilled water, and T represents tobacco leaves sprayed with solution. DETAILED DESCRIPTION

[0020] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below in conjunction with the accompanying drawings. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0021] The invention discloses an exogenous spraying liquid for tobacco leaves. The exogenous spraying liquid is prepared by mixing an EBR solution, an inositol solution and a Tween 80 solution. The EBR, inositol and Tween 80 are prepared in a volume ratio of 80-90:2-10:1-5. The concentration of the EBR solution is 1µmol / L, and the preparation method thereof is as follows: EBR is first dissolved in 3mL of anhydrous ethanol to prepare a mother liquor, and then diluted with distilled water to a concentration of 1µmol / L. The concentration of the inositol solution is 100 µmol / L, and inositol is directly dissolved in distilled water to a concentration of 100 µmol / L. The concentration of the Tween 80 solution is 0.1mg / L, and a 0.1mg / L Tween 80 solution is prepared by using a 50% ethanol solution as a solvent.

[0022] Select tobacco plants in the mature stage, spray the exogenous spray solution on the leaves two weeks before harvesting, and use the "442" ten key stable temperature point baking process (honey sweet fragrance ecological zone) (authorization announcement number CN109998144B) to bake the tobacco leaves after harvesting, ultimately improving the coordination of conventional chemical components of flue-cured tobacco and improving the quality of flue-cured tobacco. Among them, tobacco leaves are tobacco leaves that are in the mature stage but not fully mature, including upper leaves and middle leaves. The spraying time is 2 to 3 times of solution on the leaves ± 3 days before the normal harvest period of tobacco leaves, with an interval of 1 to 3 days between spraying solutions. Choose a clear and windless morning or evening, and use a sprayer to spray the solution evenly on the front and back of a single tobacco leaf until the front and back of each tobacco leaf are soaked and begin to drip the solution. After spraying, follow the local conventional field management methods. Harvest the tobacco leaves one week later, let the poles dry for 12 hours and then ignite them. Use the "442" ten key stable temperature point baking process (honey sweet fragrance ecological zone) to bake the tobacco leaves.

[0023] The present invention will be further described below in conjunction with specific embodiments and comparisons.

[0024] Example 1: Method for promoting the maturity of tobacco leaves in the middle of the honey-sweet aroma ecological zone and improving the quality of flue-cured tobacco by spraying the leaves before harvesting Follow these steps in order: (1) Within 14 days before the normal harvest period, the middle leaves of “Yunyan 87” tobacco that were not fully mature and growing in the field were selected. The SPAD value of the tobacco leaves was measured using a chlorophyll meter (SPAD-502Plus, Zhejiang Top Yunnong Technology Co., Ltd.).

[0025] (2) Prepare 1 µmol / L EBR solution (EBR is first dissolved in 3 mL of anhydrous ethanol to prepare a stock solution, and then diluted with distilled water to a concentration of 1 µmol / L), 100 µmol / L inositol solution (inositol is directly dissolved in distilled water to a concentration of 100 µmol / L), and 0.1 mg / L Tween 80 solution (0.1 mg / L Tween 80 solution is prepared using 50% ethanol solution as a solvent). Prepare the solution in the ratio of 85 mL EBR solution: 5 mL inositol solution: 3 mL Tween 80 solution. First add the inositol solution to the EBR solution and mix thoroughly. Then add the Tween 80 solution to the mixed solution and mix thoroughly. Then load it into a manual sprayer.

[0026] (3) 14 days before the normal harvest period, use a manual sprayer to evenly spray the solution on both sides of the leaves of a single tobacco plant for the first time on a clear, rainless evening until the front and back of each tobacco leaf are soaked and begin to drip. Then spray the solution once every two days for a total of three times.

[0027] (4) After spraying the solution on the leaves three times, the tobacco leaves were harvested one week later according to the local conventional field management methods, and the SPAD value and color difference of the tobacco leaves were measured. Figures 1 to 3 As shown, it was found that the SPAD values ​​of the fresh tobacco leaves of the control (CK, spraying the same amount of clean water in the same field as the control) and the treatment (T) were significantly lower than those of the fresh tobacco leaves before treatment; the SPAD value of the fresh tobacco leaves treated with T was significantly lower than that of CK, and a* and b* were significantly higher than those of CK, and the tobacco leaves turned yellow more completely.

[0028] (5) After the middle tobacco leaves are harvested, they are braided and air-dried for 12 hours before being ignited. The tobacco leaves are then baked using the "442" ten key stable temperature point baking process (honey sweet aroma ecological zone).

[0029] (6) After the tobacco is baked, the chemical substances are tested using a flow analyzer. The results are as follows: Figure 4 As shown in the data, compared with CK, the total sugar content of flue-cured tobacco after T treatment was higher, and the sugar-alkali ratio, chlorogenic acid, and potassium content were significantly higher than those of CK, while the total alkaloids, starch, protein, and chlorine contents were significantly lower than those of CK, which improved the coordination of the chemical components of flue-cured tobacco and enhanced its quality.

[0030] (7) After the tobacco is cured, it is handed over to experts and local farmers for appearance quality evaluation. Figure 5 As shown in the figure, the chromaticity evaluation score of the flue-cured tobacco treated with T was the same as that of CK, and the color, maturity, structure, identity and oil content evaluation scores were all higher than those of CK. After comprehensive calculation, the overall evaluation score of the treatment with T was higher than that of CK.

[0031] Example 2: Method for promoting the maturity of upper tobacco leaves in the honey-sweet aroma ecological zone and improving the quality of flue-cured tobacco by spraying before harvesting Follow these steps in order: (1) Thirteen days before the normal harvest period, the upper leaves of "Yunyan 87" tobacco that were not fully mature and were growing in the field were selected, and the SPAD values ​​of the tobacco leaves were measured using a chlorophyll meter.

[0032] (2) Prepare 1 µmol / L EBR solution (EBR is first dissolved in 3 mL of anhydrous ethanol to prepare a stock solution, and then diluted with distilled water to a concentration of 1 µmol / L), 100 µmol / L inositol solution (inositol is directly dissolved in distilled water to a concentration of 100 µmol / L), and 0.1 mg / L Tween 80 solution (0.1 mg / L Tween 80 solution is prepared using 50% ethanol solution as a solvent). Prepare the solution in the ratio of 83 mL EBR solution: 7 mL inositol solution: 2 mL Tween 80 solution. First add the inositol solution to the EBR solution and mix thoroughly. Then add the Tween 80 solution to the mixed solution and mix thoroughly. Then load it into a manual sprayer.

[0033] (3) 13 days before the normal harvest period, use a manual sprayer to evenly spray the solution on both sides of the leaves of individual tobacco plants for the first time on a clear, rainless evening until the front and back of each tobacco leaf are soaked and begin to drip. Then spray the solution once every three days, for a total of two sprays.

[0034] (4) After foliar spraying twice, the conventional field management method was followed. The tobacco leaves were harvested one week later and the SPAD value and color difference of the tobacco leaves were measured. The study found that the SPAD values ​​of fresh tobacco leaves treated with CK (using the same amount of water sprayed on the same field as the control) and T were significantly lower than those of fresh tobacco leaves before treatment; the SPAD value of fresh tobacco leaves treated with T was significantly lower than that of CK, and a* and b* were significantly higher than those of CK, and the tobacco leaves fell yellow more fully. Figures 6 to 8 .

[0035] (5) After harvesting the upper tobacco leaves, the tobacco leaves are braided and air-dried for 12 hours before being ignited. The tobacco leaves are then baked using the "442" ten key stable temperature point baking process (honey sweet aroma ecological zone).

[0036] (6) After the tobacco was cured, it was handed over to experts and local farmers for appearance quality evaluation. The color and chromaticity evaluation scores of the T treatment were the same as those of the CK, while the maturity, structure, oil content, and identity evaluation scores were higher than those of the CK. After comprehensive calculation, the overall evaluation score of the T treatment was higher than that of the CK. Fig. 9 .

[0037] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A tobacco leaf exogenous spraying liquid, characterized in that: The exogenous spraying liquid is prepared by mixing EBR solution, inositol solution and Tween 80 solution.

2. The tobacco leaf exogenous spraying liquid according to claim 1, characterized in that: In the exogenous spraying liquid, EBR, inositol and Tween 80 are mixed in a volume ratio of 80-90:2-10:1-5.

3. The tobacco leaf exogenous spraying liquid according to claim 1, characterized in that: The concentration of the EBR solution is 1 µmol / L, and the preparation method thereof is as follows: EBR is first dissolved in 3 mL of anhydrous ethanol to prepare a mother solution, and then diluted with distilled water to a concentration of 1 µmol / L.

4. The tobacco leaf exogenous spraying liquid according to claim 1, characterized in that: The concentration of the inositol solution is 100 µmol / L.

5. The tobacco leaf exogenous spraying liquid according to claim 1, characterized in that: The concentration of the Tween 80 solution is 0.1 mg / L.

6. Use of the tobacco leaf exogenous spray liquid according to any one of claims 1 to 5 in promoting the maturity of pre-harvest tobacco leaves and improving the quality of flue-cured tobacco.

7. The use according to claim 6, characterized in that: Select tobacco leaves at the maturity stage and spray the tobacco leaf exogenous spray liquid on the leaf surface before harvesting.

8. The use according to claim 7, characterized in that: The tobacco leaves are tobacco leaves that are in the maturity stage but not fully mature, including upper leaves and middle leaves.

9. The use according to claim 7, characterized in that: The spraying time is 2 to 3 times of spraying the solution on the leaves within two weeks ± 3 days before the normal harvest period of tobacco leaves, with an interval of 1 to 3 days between spraying the solution.

10. The use according to claim 7, characterized in that: The tobacco leaves are sprayed with the solution evenly on both sides of the leaves of a single tobacco plant using a sprayer in the clear and windless morning or evening until both sides of each tobacco leaf are soaked and begin to drip with the solution.

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