125results about How to "Early flowering" patented technology

The invention discloses an endophytic fungi chaetomium globosum strain, a microbial agent and an application thereof. The strain is named as chaetomium globosum strain ND 35, the preservation organization is the China General Microbiological Culture Collection Center, the preservation date is 9th,10,2010, the preservation number is CGMCC No. 4136. The strain is a biological beneficial bacteria with a plurality of biological control effect mechanisms, and has the plant pathogen inhibition capability with broad-spectrum and high-efficiency, and also has capability for promoting a plurality of plants growth, and is suitable for industrial production, the shelf life is long which is suitable for preventing and treating the plant diseases, so that the strain is used for producing the biological bactericide products for preventing and treating a plurality of plant diseases and multifunctional biofertilizer products for promoting the growth of plants.

The present invention is directed to an isolated DNA molecule encoding a Xanthomonas campestris hypersensitive response elicitor protein or polypeptide. The DNA molecule that encodes such protein has the following uses: imparting disease resistance to plants, enhancing plant growth, controlling insects on plants, imparting stress resistance, imparting post-harvest disease resistance, maximizing the benefit of or overcoming a yield penalty associated with a transgenic trait, inhibiting desiccation of cuttings from ornamental plants, and promoting early flowering of an ornamental plant. These can be achieved by expression of the hypersensitive response elicitor in transgenic plants. Transgenic plants, plants seeds, and cuttings from such transgenic plants are also disclosed.

The invention provides a gene with a zinc finger protein structure BBX24 and application thereof. The invention also provides a nucleotide sequence encoded with a zinc finger protein transcription factor and application in regulation of plant flowering time and abiotic stress tolerance. The gene silencing of the zinc finger protein transcription factor on chrysanthemum shows insensitivity to a photoperiod, can obviously make the flowering time of short-day chrysanthemum ahead of schedule under a long sunshine condition, and the overexpression on chrysanthemum shows enhanced tolerance to low temperature and drought. Therefore, the gene has unique advantages in the application of regulating plant flowering time and abiotic stress tolerance.

The invention relates to a strawberry flowering gene FvbHLH78 and an application thereof, and belongs to the field of genetic engineering in molecular biology. The strawberry flowering gene FvbHLH78 is characterized in that a full-length coding region sequence of strawberry FvbHLH78, which is capable of promoting precocious flowering, is shown as SEQ ID NO: 1 in a sequence list; and an amino acid sequence is shown as SEQ ID NO: 2 in the sequence list. According to the strawberry flowering gene FvbHLH78 and a plant expression vector for constructing the FvbHLH78 gene provided by the invention, a transgenic arabidopsis plant can be acquired and precocious flowering can be promoted as the constructed plant expression vector is transformed into arabidopsis through agrobacterium, showing that the gene has a function of promoting the precocious flowering of the arabidopsis. The invention, on the basis of genetic engineering technology, can offer theoretical basis and technological means for regulating and controlling flowering time of strawberries; therefore, a high application value is guaranteed.

The invention relates to a culture method of hyacinth virus-free tissue culture bulb, which belongs to plant propagation technical field. The method comprises the steps of the preparation of a culture medium, the culture of the virus-free tissue culture bulb, the detection of a virus ELISA and so on. The invention can detect tissue culture bulb seedlings rapidly with high sensitivity and can stop the virus from carrying and transmission. Comparing with the bulbs which are not virus-free, the virus-free tissue culture bulb has rapid growth speed, early flowering stage and bright flower colour. Virus-free tissue culture bulb breeds rapidly, the breeding ratio per month of which can reach 3-5 times more than the bulbs which are not virus-free. The invention is suitable for industrial seedling production and can be widely used in the purification, the rejuvenescence and the development of the hyacinth.

The invention discloses a method for air layering propagation of calotropis gigantea. The method is characterized by comprising the steps of branch selection, substrate preparation and processing, air layering, layering post-management and in-vitro transplanting; upright or inclined robust branches with the diameter being 0.5-1.5 cm are selected on seed trees of the calotropis gigantea, girdling is performed, a prepared substrate A is bound on a girdling opening, layering post-management is performed, and finally in-vitro transplanting culture is performed to obtain high-quality calotropis gigantea calotropis gigantea seedlings. According to the method, layering rooting is rapid, the rooting rate is 100%, the transplanting survival rate reaches up to 92% or above, seedlings are robust and high in quality, and calotropis gigantea seedlings with the good-character female parent can be reserved within a short period of time; meanwhile, seed transplanted seedlings flower and fruit before seed seedlings, high-quality, efficient, large-scale and rapid development of calotropis gigantea can be promoted, and good economic, social and ecological benefits are achieved.

The invention discloses protein MsLEA-D34 of medicago sativa 'WL525' at late embryonic development period and a coding gene of the protein MsLEA-D34, and relates to the field of plant genetic engineering. The protein MsLEA-D34 comprises polypeptide as shown in an amino acid sequence SEQID NO:2, polypeptide in variation form of the polypeptide, active fragments of the protein MsLEA-D34 and active derivatives; and besides, the invention provides the coding gene of the protein MsLEA-D34, and the coding gene comprises a nucleotide sequence as shown in nucleotide sequence SEQID NO:1, degenerate sequences and variation sequences of the nucleotide sequence SEQID NO:1, and a sequence hybridized with the nucleotide sequence SEQID NO:1. According to the protein MsLEA-D34 and the coding gene disclosed by the invention, through a genetic engineering technique, cultivation of MsLEA-D34 transgenic plants is realized, adjustment of flowering time and increase of stress resistance of the MsLEA-D34 transgenic plants can be realized, an important theory basis is provided for cultivation of medicago sativa new species, adjustment and control of the flowering time and breeding work, and the protein MsLEA-D34 has great application value.

The invention relates to the technical field of rice planting, in particular to a cultivating method under LED artificial light environment, suitable for the whole bearing period of rice. The cultivating method comprises the following steps of (1) performing disinfection and seed soaking treatment on rice seeds, and spraying the treated rice seeds in a seedling raising tray; (2) placing the seedling raising disk in a darkroom, and performing breeding and germination acceleration; (3) after seed embryos are germinated, placing the seeds in nutritive soil of the seedling raising tray, and performing culture at 24-26 DEG C to obtain seedlings, wherein the surface illumination intensity of the nutritive soil in the tray is 150-250 [mu]mol.m<-2>.s<-1>, and the illumination period is 12-16h/d; (4) performing division transplantation on the seedlings, guaranteeing room temperature to be 24-26 DEG C, guaranteeing that the intensity of leaf surface light quanta reaches 300-450 [mu]mol.m<-2>.s<-1>, and guaranteeing that the illumination period is 12-16h/d; (5) when rice enters the young ear development period, guaranteeing that the quantity of the leaf surface light quanta reaches 500-800 [mu]mol.m<-2>.s<-1>, and guaranteeing that the illumination period is 12-16h/d; (6) when the rice of short-day variety enters the pre-blooming period, guaranteeing that the illumination period is 8-12h/d; and (7) when rice ears are mature, performing seed harvesting.

A method for breed strawberry seedlings save labor includes such steps as propagating, refrigerate, classifying, transplanting and activating seedlings. The invention improves the breeding coefficientof seedlings, promotes flower bud differentiation, and simultaneously improves the consistency of seedling quality and seedling age. This method is easy to operate, easy to master, repeatable, time-saving, labor-saving, easy to classify and centralized management, perfecting the shortcomings of conventional strawberry seedling.

The invention relates to a flower forcing method for passion fruit seedlings. The method includes the steps that 1, the seedlings are cultured until the age of the seedlings is seven months, and the length of seedling bodies is about two meters; 2, agriculture trimming is performed two times, and growth of first-level vines and growth of second-level vines are promoted; 3, with leaf critical withering being limit control, water treatment is performed for 6-10 days; 4, growth regulation and fertilizer application treatment of plants are performed,paclobutrazol is used for spraying for 5 days, and rapin and monopotassium phosphate are used for mixed spraying. Within 7-15 days after growth regulation and fertilizer application treatment, namely, buds start to appear from the fourth month after transplanting, normal blossoming and fruit bearing are realized, flowering is earlier by about three months than that in planting of common passion fruit seedlings, and the flower forcing method issimple, easy to implement, low in popularization cost and remarkable in economic benefit.

The invention discloses an artificial light environment cultivation method for Arabidopsis thaliana, the artificial light environment cultivation method for Arabidopsis thaliana comprises the following steps: (1) after disinfection treatment, Arabidopsis thaliana seeds are applied to a MS culture medium by a micro sampling syringe; (2) after the Arabidopsis thaliana seeds are completely applied to the MS culture medium, the MS culture medium is sealed and cultured in a dark room; (3) the MS culture medium is 55 to 65-degree inclined for culturing at 24 to 26 DEG C for 15-20d to obtain seedlings; (4) the seedlings obtained from the MS culture medium in the step (3) are taken out, and transplanted to a seeding tray with a mixed matrix; after the transplantation, the seeding tray is covered with a film, when plant leaves obviously elongate, and stems begin to develop, the plastic film is taken off, and a nutrition solution is regularly poured to ensure an adequate nutrition matrix; and (5) when pods turn yellow and mature, seeds are harvested. According to the cultivation method, in seedling raising and field planting phases, specific light environmental conditions are given to plants, the planted arabidopsis thaliana plants are strong, flowering time is early, the number of the pods is great, and the pods are full.

The invention relates to a southern propagation and northern breeding method for plug seedlings of strawberries. The southern propagation and northern breeding method comprises the following steps: 1)breeding strawberries in a warm and suitable area in the south in spring to grow a great number of healthy creeping stems; and 2) transporting the creeping stems to a cold and suitable area in the north in summer, and breeding the creeping stems by using a plug seedling raising method until the creeping stems blossom and bear fruits. According to the southern propagation and northern breeding method, the creeping stems of the strawberries are propagated and bred by sufficiently utilizing the warm climate in a specific region in the south in spring, and then, blossom buds of the seedlings of the strawberries are promoted to differentiate by utilizing advantages of the climate in a cold area of a specific region in the north in summer.

The invention discloses a treatment method for promoting flowers and protecting flowers of syzygium samarangense saplings. The method comprises the steps of: when new tips of syzygium samarangense saplings in spring sprout, manually removing the tips, stopping the tip removal work till the flower buds are 0.6 cm*0.8 cm in size, and letting out shoots; from the next day after removal of the new tips for the first time, spraying the whole tree by virtue of a mixed diluent of boric acid and potassium dihydrogen phosphate till water drops, and spraying the diluent once every ten days for continuously three times; and carrying out rest of operations according to normal daily management of a syzygium samarangense garden. The method and a agent formula disclosed by the invention are scientific and reasonable, the implementation method is simple, the implementation cost is low and the flower promoting and protecting effects are obvious, and the fruits are excellent in quality, and the output increase is obvious, so that the method has a remarkable economical benefit and is suitable for being popularized and applied on a large scale.

The invention relates to a strawberry transcription factor for promoting precocious flowering of plants and application thereof, and belongs to the field of genetic engineering in molecular biology. The strawberry transcription factor is characterized in that a full-length coding region sequence of the strawberry WRKY46 gene for promoting precocious flowering of the plants is shown in the sequence table SEQ ID NO:1; an amino acid sequence is shown in the sequence table SEQ ID NO:2. The strawberry FvWRKY46 gene for promoting precocious flowering of the plants is used for constructing plant expression vectors of the FvWRKY46 gene, the constructed plant expression vectors transform arabidopsis thaliana through agrobacteria, genetically modified arabidopsis thaliana plants are obtained and flower early, and it is shown that the FvWRKY46 gene has the function of inducing precocious flowering of arabidopsis thaliana. According to the strawberry transcription factor for promoting precocious flowering of the plants, a genetic engineering technology is adopted, technical means and theoretical bases are provided for regulating the flowering time of strawberries, and the strawberry transcription factor has great application value.

The invention provides a seedling culturing method for promoting early flowering and fruiting of strawberries, and belongs to the field of agricultural planting. Strawberry virus-free seedlings with the excellent characters are selected as mother plants, and strawberry stolon is cultured in a soilless culture mode; factors such as illumination, the temperature, the nutrient solution concentrationand growth substrates at different growth stages are controlled, the early flowering and fruiting effect is achieved, the yield is increased, the quality is improved, the taste and the nutritive valueof the strawberries are improved, and picking time is prolonged.

The invention provides isolated floral transition nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering floral transition in plants. The invention further provides regulatory elements, recombinant expression cassettes, host cells, and transgenic plants.