A novel soybean variety, designated 5PLSL31 is provided. Also provided are the seeds of soybean variety 5PLSL31, cells from soybean variety 5PLSL31, plants of soybean 5PLSL31, and
plant parts of soybean variety 5PLSL31. Methods provided include producing a soybean
plant by crossing soybean variety 5PLSL31 with another soybean
plant, methods for introgressing a transgenic trait, a
mutant trait, and / or a native trait into soybean variety 5PLSL31, methods for producing other soybean varieties or plant parts derived from soybean variety 5PLSL31, and methods of characterizing soybean variety 5PLSL31. Soybean seed, cells, plants,
germplasm, breeding lines, varieties, and plant parts produced by these methods and / or derived from soybean variety 5PLSL31 are further provided.