Artificial light environment cultivation method for Arabidopsis thaliana

Abstract

The invention discloses an artificial light environment cultivation method for Arabidopsis thaliana, the artificial light environment cultivation method for Arabidopsis thaliana comprises the following steps: (1) after disinfection treatment, Arabidopsis thaliana seeds are applied to a MS culture medium by a micro sampling syringe; (2) after the Arabidopsis thaliana seeds are completely applied to the MS culture medium, the MS culture medium is sealed and cultured in a dark room; (3) the MS culture medium is 55 to 65-degree inclined for culturing at 24 to 26 DEG C for 15-20d to obtain seedlings; (4) the seedlings obtained from the MS culture medium in the step (3) are taken out, and transplanted to a seeding tray with a mixed matrix; after the transplantation, the seeding tray is covered with a film, when plant leaves obviously elongate, and stems begin to develop, the plastic film is taken off, and a nutrition solution is regularly poured to ensure an adequate nutrition matrix; and (5) when pods turn yellow and mature, seeds are harvested. According to the cultivation method, in seedling raising and field planting phases, specific light environmental conditions are given to plants, the planted arabidopsis thaliana plants are strong, flowering time is early, the number of the pods is great, and the pods are full.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to an artificial light environment cultivation method of Arabidopsis thaliana. Background technique [0002] Arabidopsis is the first higher plant in the world to complete the determination of the entire genome sequence. As a model plant for studying the genetics, cells, development, and molecular biology of flowering plants, it has the advantages of small plants, self-pollination, many seeds, The gene is highly homozygous, the mutation rate is high when treated with physical and chemical factors, and it is easy to obtain defective types of various metabolic functions. These irreplaceable features make it very important to optimize the planting of Arabidopsis in the laboratory. Indoor cultivation can not only obtain the materials needed for the experiment, but also break the restriction of the natural environment on Arabidopsis. Among all environmental factors...

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Problems solved by technology

However, there are the following problems in the existing research: (1) The selected artificial light quality is single, only mentioning red light or blue light or red and blue light, and no reasonable and scientific full-spectrum structure is proposed, especially green light, let alone spectrum The proportion of energy in different wavelength spectrums, and did not propose the specific parameters of the light environment required for different growth stages: including spectrum and light intensity; (2) only study a certain stage of the entire growth and cultivation process of Arabidopsis, such as seed germination, No systematic and comprehensive cultivation methods for Arabidopsis thaliana have been proposed; the existence of these problems makes it difficult to efficiently grow Arabidopsis indoors using artificial light sources

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