Application of cotton GhCAL-D07 gene in promotion of plant blooming

A technology of ghcal-d07 and 1.ghcal-d07 is applied in the application field of GhCAL-D07 gene in promoting plant flowering, which can solve problems such as long cycle and achieve the effect of advancing flowering time

Active Publication Date: 2019-08-13
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the shortcoming of long cycle in conventional breeding technology, molecular breeding has become the trend of the times, and the transgenic method is an effective method for cultivating new varieties, so the inventors are committed to developing new genes

Method used

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  • Application of cotton GhCAL-D07 gene in promotion of plant blooming
  • Application of cotton GhCAL-D07 gene in promotion of plant blooming
  • Application of cotton GhCAL-D07 gene in promotion of plant blooming

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Cotton material

[0028] The cotton materials selected in this example are Upland cotton 50, Zhongmian Institute 74, Guoxin cotton No. 11, and Bomian No. 1, which were planted in the State Key Laboratory of Cotton Biology, Cotton Research Institute, Chinese Academy of Agricultural Sciences (Anyang Baibi) , the management measures are normal field management.

[0029] 2. Reagents and consumables

[0030] 2.1 Enzymes and kits: GXL DNA Polymerase High-Fidelity Enzyme, Gel Recovery Kit, and PCR Product Purification Kit were purchased from Takara Company; RNA Reverse Transcription Kit, KOD FX Neo Enzyme (Code.No.KFX-201) were purchased from Toyobo Company; The Ultra One Step Cloning Kit kit was purchased from Vazyme Company; a small amount of plasmid extraction kit was purchased from Magen Company; restriction endonucleases (BamH I, Sac I) were purchased from NEB Company; DNAMarker III and plant total RNA extraction kits were purchased from TIANGEN Company; fluores...

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Abstract

The invention discloses application of a cotton GhCAL-D07 gene in promotion of plant blooming and belongs to the technical field of plant gene engineering. The GhCAL-D07 gene comprises a nucleotide sequence shown in SEQ ID NO:1 and can code a nucleotide sequence shown in SEQ ID NO:2. According to the application, through a transgenic technology, an arabidopsis plant with the trans-GhCAL-D07 gene is obtained, so that blooming of the arabidopsis is obviously advanced. Furthermore, by silencing the GhCAL-D07 gene in cotton, it is proved through a result that the GhCAL-D07 gene has a key functionon promotion of cotton blooming. According to the application, beneficial gene resources are provided for cultivation of short-season cotton.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to the application of GhCAL-D07 gene in promoting flowering of plants. Background technique [0002] Eating and dressing are important matters for people's livelihood, but our country is faced with the basic national conditions of more people and less land, so there is a serious contradiction between grain and cotton land. In order to alleviate this contradiction, breeders are committed to the cultivation of short-season cotton and realize a The farming system of double cropping of wheat and cotton in one year. Due to the shortcoming of long cycle in conventional breeding technology, molecular breeding has become the general trend, and the transgenic method is an effective method for cultivating new varieties, so the inventors are committed to developing new genes. [0003] Short-season cotton refers to upland cotton varieties with a short growth perio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/60A01H6/20A01H6/46
CPCC07K14/415C12N15/827
Inventor 魏恒玲程帅帅喻树迅王寒涛马亮苏政政
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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