Ultrahigh permeability bread yeast and preparation method thereof

Abstract

The invention discloses an ultrahigh permeability bread yeast and a preparation method thereof, and relates to a pattern biological bread yeast, which can be used for detecting environmental genotoxic carcinogen. Through reconstruction of genetic gene technology, the yeast has improved permeability, so that the yeast has wider detection range and higher sensitivity to environmental pollutants. Through gene knock-out technology, genes CWP1 and CWP2, which are used for encoding protein mannose protein serving as an important structure of bread yeast cell wall, is inactivated to improve the permeability of the bread yeast cell wall to environmental carcinogen; and genes SNQ2 and PDR5, which are used for encoding an efflux pump protein on a cell membrane of bread yeast, is inactivated to improve the accumulation of the environmental pollutants in the bread yeast cell. Through the integration of two reconstruction strategies, an RNR3-1acZ system is constructed when four genes CWP1, CWP2, SNQ2 and PDR5 knock out bread microzyme, which can improve the sensitivity of the system to detecting the environmental genotoxic carcinogen.

Description

Technical field: [0001] The invention relates to biotechnology, in particular to the monitoring of environmental pollutants by genetically modified microorganisms. Background technique: [0002] Baker's yeast is a simple eukaryotic organism with single cells, simple genetic modification, easy and fast cultivation and environmental friendliness. Baker's yeast is not only the dominant strain in the fermentation industry, but also a model organism widely used in life science research. At present, in the field of environmental pollution detection technology, baker's yeast, as a simple eukaryote, has been paid more and more attention, and some detection systems using baker's yeast as a model organism have been developed. However, the permeability of yeast cells is the bottleneck that restricts the development of yeast cells for environmental pollution detection. [0003] Pollutant macromolecules in the environment usually exist in low concentrations. Due to the permeability bar...

AI Technical Summary

Problems solved by technology

However, the permeability of yeast cells is a bottleneck that restricts the development of yeast cells for environmental pollution detection.

[0003] Pollutant macromolecules in the environment usually exist in low concentrations. Due to the permeability barrier of the baker's yeast cell wall and the multidrug resistance pathway of the cell membrane, the toxic substances in the cell can be excreted, which limits the presence of environmental macromolecular pollutants in the baker's yeast cells. Therefore, the sensitivity of the environmental pollution detection system using baker's yeast as a model organism is reduced; some chemicals with large molecular weights cannot even freely pass through the cell wall of baker's yeast and cannot be detected by the system

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