Individual administration gene type diagnostic chip and its producing method and using method

Abstract

The present invention relates to gene technology field, and is gene diagnosis chip for individual administration and its making process and usage. The gene diagnosis chip includes bearing substrate and gene probes distributed on the substrate, and the gene probes contain oligonucleotide sequences complementary with the medicine metabolism and effect related gene mutant sites and have reference probes set. Each mutant site has two types of probes, wild type and mutant type, designed. The chip making process includes designing two types of probes of length 17-22 bp for each mutant site, synthesizing probes with DNA sequence synthesizing instrument, applying probes onto the substrate with chip sample applying instrument, curing the chip and storing at 4 deg.c. The usage of the gene diagnosis chip includes sampling, sample processing, PCR amplification, crossbreeding, washing, reading, result analysis and other steps.

Description

technical field [0001] The invention belongs to the field of gene technology, and relates to a genotype diagnosis chip for individualized medicine and its manufacturing method and application method. Background technique [0002] Gene chip (Gene chip), also known as DNA chip (DNA CHIP), DNA array (DNA arrays), oligonucleotide microchip (oligonucleotide micro-chip), refers to the use of many specific oligonucleotide fragments or gene fragments as The probes are regularly arranged and fixed on the support, and then hybridized with the gene of the labeled sample to be tested according to the principle of base pairing, and then the chip is scanned by the detection system, and the intensity of the hybridization signal is determined by the computer system. Analyze and process to get the desired information quickly. At present, gene chip technology is being gradually applied to various fields of life science. [0003] Gene chip preparation technology: The essence of a gene chip i...

AI Technical Summary

Problems solved by technology

[0011] Defects of existing clinical medication methods: In the treatment of many diseases, side effects caused by improper medication often occur or the therapeutic purpose cannot be achieved due to insufficient dosage

If it is suspected that the patient may be sensitive to a certain drug due to genetic factors, a series of operations such as DNA extraction, PCR amplification, and restriction nuclease endonuclease are required, which is not conducive to simultaneous detection of multiple gene mutations

This process of adjusting medication is complicated, time-consuming, and delays the timing of treatment.

Images