23 results about "Gene bank" patented technology

The invention discloses a kelp rehydration characteristic prediction and segmented drying intelligent regulation and control system and method, and relates to the field of kelp rehydration characteristic prediction and segmented drying intelligent regulation and control, and the method comprises the steps: extracting parameter characteristics affecting the moisture content of dried kelp, and collecting parameter characteristic data in real time; obtaining values of water content of dried kelp under different parameter characteristics, and establishing a kelp drying data set; constructing a kelp drying mapping model, and predicting and obtaining the moisture content of the dried kelp; based on a genetic algorithm, obtaining an optimal combination of the temperature, the humidity, the pH value, the wind speed and the drying time; establishing a kelp rehydration characteristic gene bank according to the original data, the model output and the professional detection data; and establishing a kelp rehydration characteristic prediction model, and predicting the kelp rehydration characteristics. The method has the advantages that parameter characteristics are collected in real time through the multi-mode sensor, drying parameters are optimized in combination with the CNN neural network and the genetic algorithm, and a gene pool is constructed to achieve accurate prediction of rehydration characteristics.

The application discloses application of a CsERF017 gene or a protein coded by the same in regulation of resistance of navel orange fruits to sour rot, and belongs to the technical field of molecular biology and fruit and vegetable preservation. A nucleotide sequence of the CsERF017 gene is shown in SEQ ID NO. 2, and an amino acid sequence of the protein coded by the same is shown in SEQ ID NO. 3. The application finds through experiments that the CsERF017 gene is up-regulated after infection of a citrus sour rot pathogen Geotrichum candidum, and silencing or knocking out the CsERF017 gene can enhance the resistance of navel orange fruits to sour rot, and the specific performance is that, compared with a control, the transgenic fruits after inoculation of the G. candidum have reduced symptoms of sour rot, and both a disease index and a TA content are reduced. The application provides an important gene bank and new insights for breeding of citrus disease-resistant varieties and development of molecular markers, and is helpful to fundamentally reduce the dependence on chemical synthetic pesticides in postharvest preservation treatment of citrus fruits.

This invention relates to the field of intelligent fault early warning technology, and discloses a nanoscale fault early warning and self-repair method based on quantum sensing and materials genome. The method includes: a detection layer: using a diamond NV color center quantum sensor to capture nanoscale lattice distortion, and fusing multimodal data through federated learning; an evaluation layer: dynamically updating the probability of fault tree nodes, combining pre-set SSC critical threshold conditions from a materials genome library, establishing a remaining lifetime prediction model, and simulating the remaining lifetime; and a repair layer: automatically triggering a repair mechanism when the quantum signal offset and AI confidence level > 90%, and verifying the repair effect. This application, through the interdisciplinary integration of quantum sensing, materials genome engineering, and industrial AI, constructs a closed-loop system of nanoscale damage detection, dynamic fault tree evaluation, and autonomous repair, solving the technical challenges of difficult nanoscale damage monitoring, poor dynamic evaluation adaptation, and delayed repair response in traditional turbochargers under extreme conditions of high sulfur, high pressure, and high volatility.

The present application relates to the technical field of machining, and discloses a segmented type bundling and stretching forming equipment for large-curvature roller coating hyperbolic aluminum plate, which comprises the following steps: establishing a distributed material gene library, designing a material parameter verification and correction mechanism, calibrating parameters, and triggering model retraining when continuous abnormalities occur; establishing an environmental influence coefficient matrix, calculating actual yield strength, strain threshold and pre-stretching force; collecting aluminum plate surface images in real time; dynamically adjusting stretching parameters, optimizing stretching speed to control stretching force loading; reconstructing a strain field in real time, and designing a self-adaptive termination mechanism; inputting material and strain parameters to predict critical curvature, calculating square tube structure parameters, calculating initial pressure and optimizing pressure; designing a multi-stage stretching and pressure maintaining process, and reducing pressure of a corresponding area if local strain exceeds a limit; controlling progressive unloading by using an exponential decay curve, detecting residual stress, performing full-size detection, analyzing deformation deviation, solving optimal parameters, and updating a parameter library.

This invention discloses a ruminant-specific regulatory element retrieval probe based on a 5' desulfurized biotin label and its application. Ruminant-specific regulatory elements are screened through cross-species whole-sequence alignment. Based on the screened gene sequences, a specific retrieval probe with a 5' desulfurized biotin label is designed and synthesized. The retrieval and enrichment process is established and optimized, enabling targeted identification of ruminants and rapid construction and acquisition of a ruminant-specific gene library. This method can simultaneously capture ruminant-specific regulatory element information at the family / genus level, providing an efficient, reliable, and simple technical means for rapidly screening and analyzing ruminant-derived components in livestock production and for identifying ruminant attributes in archaeological skeletal and fossil samples, supporting research on the domestication history of ruminants. The rapidly constructed ruminant-specific gene library provides a genetic basis for analyzing unique ruminant traits, significantly accelerating the traditional process of functional enrichment and pathway enrichment stepwise analysis.

The present invention provides a high-temperature inducible plant promoter pAcHSR, the nucleotide sequence of which is shown in SEQ ID NO.1. The present invention also provides a recombinant expression vector and a recombinant bacterium containing the above promoter pAcHSR, respectively. Meanwhile, the present invention provides an application of the promoter pAcHSR in cultivating plants resistant to high-temperature stress and a method for cultivating plants resistant to high-temperature stress. The present invention screened and finally determined the high-temperature inducible plant promoter pAcHSR in the kiwifruit gene bank; the promoter pAcHSR of the present invention drives the transcription and translation of the heat shock transcription factor AcHSFA2-1 through high-temperature induction, improves the high-temperature stress resistance of plants, and has great application prospects for cultivating plants resistant to high-temperature stress and creating plant germplasm resources with high resistance to cope with global climate change.

The invention discloses a multi-satellite joint area coverage planning method based on a convex hull, and aims to solve the problems of high calculation complexity and low multi-satellite cooperation efficiency of a complex area observation task, and the method comprises the steps: obtaining a coordinate set of area boundary points, traversing all boundary point sets through a convex hull algorithm, and finding out a plurality of convex hull vertexes to construct a convex hull vertex set; the method comprises the following steps: acquiring satellite orbit data, traversing a convex hull vertex set, designing a satellite-region pre-screening strategy, calculating region key feature points through convex hull vertexes and orbit data, and constructing a feasible space-time envelope; the method comprises the following steps: acquiring satellite historical observation data, introducing a knowledge base data-driven optimization task construction method, determining an adaptive discretization interval based on knowledge base data, generating sub-region observation tasks based on space-time envelope division, and constructing a satellite-region-observation task gene pool; and performing iterative optimization based on the gene pool by using an improved genetic algorithm to obtain a task planning scheme. According to the method, the calculation amount generated by a multi-satellite joint observation area task scheduling algorithm can be reduced, it is ensured that multiple satellites can efficiently and cooperatively complete the area observation task, and the planning efficiency is improved.

The present disclosure provides a mutant strain of Aspergillus niger designated MTCC 25761 on deposit with the Microbial Type Culture Collection and Gene Bank, Institute of Microbial Technology, Chandigarh, derived from parent strain Aspergillus niger ATCC 9142 through UV mutagenesis at 254 nm wavelength for 19 minutes with constant stirring The mutant strain demonstrates enhanced production capabilities for both citric acid and gluconic acid compared to the parent strain. The mutant strain exhibits enhanced metabolic performance through improved enzyme expression, substrate utilization efficiency, and regulatory control mechanisms. Gluconic acid production is 1.37-fold higher (164.045 g / L) and citric acid production is 1.29-fold higher (0.506 g / L) than the parent strain.

The invention discloses a clubroot-resistant brassica juncea gene BjuA03. AP and application thereof, and relates to the technical field of plant biology. The BjuA03. AP gene is obtained through a homologous cloning method, BjuA03. AP gene overexpression vector genetic transformation is performed on brassica juncea / brassica napus through an agrobacterium-mediated method, and the brassica juncea / brassica napus with clubroot resistance remarkably higher than that of wild brassica juncea / brassica napus can be obtained. According to the invention, a BjuA03. AP overexpressed brassica juncea / brassica napus plant is obtained through genetic transformation; not only is a clubroot resistance gene pool widened, but also a new variety of excellent brassica napus with broad-spectrum resistance is cultivated.

The invention relates to the technical field of plant biology, in particular to application of eucalyptus grandis EgrDof5 gene in poplar, and the application comprises at least one of the following items: a, improving poplar biomass; b, improving photosynthetic performance of the poplar; c, regulating and controlling secondary growth of the poplar; the nucleotide sequence of the eucalyptus grandis EgrDof5 gene is as shown in SEQ ID No. 1. By transferring the EgrDof5 gene into the populus genome, the poplar biomass can be increased, the photosynthetic performance of the poplar can be improved, the secondary growth of the poplar can be regulated and controlled, a new method is provided for genetic improvement of the poplar, the gene bank of the poplar can be enriched, and more choices are provided for forest breeding in the future.

This invention discloses a miR204 derived from *Mustela stenoptera* and its applications. The mature sequence of miR204 is shown in SEQ ID NO. 1, and its precursor sequence is shown in SEQ ID NO. 2. This invention is the first to discover *Mustela stenoptera* miR204 and demonstrates through molecular genetic methods that this miRNA not only affects plant resistance to drought but also has a dual function of regulating plant salt tolerance. This research not only enriches the plant stress resistance gene pool and provides a key target gene for plant genetic improvement but also offers a new option for breeding high-quality stress-resistant varieties. It also provides reliable materials and data support for research on the molecular biological mechanisms of enhancing the stress resistance of *Mustela stenoptera*.

The invention provides a local livestock and poultry traceability method based on an SNP marker, and relates to the field of variety traceability, and the method comprises the steps of traceability gene bank construction, genetic relationship determination, traceability identification based on genome data, and traceability identification process and result determination. Wherein the traceability gene pool construction comprises source gene pool sample collection, sample collection and information recording, gene analysis and data generation, and traceability gene pool establishment; the genetic relationship judgment comprises repeated individual identification and paternity test; the traceability identification based on the genome data comprises repeated individual identification based on genome information and parent-child relationship identification based on the genome information; the traceability identification process and result judgment comprises sample receiving and quality control, data comparison and analysis and hierarchical result judgment. According to the method, SNP marker information is obtained through a genomics technology, accurate source tracing of livestock and poultry meat products is achieved through genetic relationship analysis, and the counterfeit and shoddy phenomena are effectively eradicated.

The present application relates to the technical field of livestock information management, and particularly relates to a livestock and poultry germplasm resource gene bank frozen semen tracking detection system, which solves the technical problem of poor tracking detection effect of the prior art. The system comprises: a risk assessment unit for performing risk assessment based on the time length data of the previous basket-out-of-can action of each frozen semen thin tube of the target batch and the spatial position information of the thin tube to determine the cumulative exposure risk characteristic value of each frozen semen thin tube of the target batch; a calibration construction unit for constructing a sparse calibration set based on the sperm survival rate obtained by testing and the cumulative exposure risk characteristic value; a threshold determination unit for performing bottom-line risk quantification analysis based on the data distortion condition of the sparse calibration set and the misjudgment loss cost to determine the batch interception threshold; and a failure detection unit for performing failure detection according to the cumulative exposure risk characteristic value of each frozen semen thin tube of the target batch and the batch interception threshold.

The invention discloses a method for identifying angelica sinensis based on ITS2 PCR combined with sequencing, and belongs to the field of traditional Chinese medicinal material detection. The method comprises the following steps: extracting DNA (Deoxyribonucleic Acid) of angelica sinensis, performing PCR (Polymerase Chain Reaction) amplification, adding A tail, performing nucleic acid electrophoresis, recovering gel, constructing a vector, converting, performing blue-white screening, extracting plasmids, performing PCR and enzyme digestion detection, finally sequencing, and comparing a gene bank to identify the authenticity of an angelica sinensis sample. The identification results of eight angelica sinensis samples show that four angelica sinensis samples, three angelica dahurica samples and one angelica dahurica sample, and the comparison similarities of ITS2 fragments of the angelica sinensis samples and a gene bank are 98.99%, 95.56% and 98.99% respectively. Therefore, the gene identification method established by the invention can accurately identify the authenticity of the angelica sinensis sample, and provides reference for angelica sinensis identification.

Modified potato starch composition obtained or available through a process comprising the following steps: f) Providing gelatinized potato starch; g) Providing one or more alpha amylases selected from the group consisting of enzyme species Genebank deposit number To uncultured marine bacterium ADK21254.1 Ac Anaerocolumna cellulosilytica WP_197978574.1 Po Petrotoga mobilis WP_012208959.1 h) Incubating the gelatinized potato starch from step a) with one or more alpha-amylases from step b), for example for a period of 5 - 180 minutes at a temperature in the range of 40 - 70°C; i) optionally inactivating one or more alpha-amylases; and j) Providing a modified potato starch composition.

The present invention discloses a Ferritin L gene of Hefang Crucian Carp and its recombinant protein. The nucleotide sequence of the Ferritin L gene of Hefang Crucian Carp is shown as SEQ ID NO: 1, and the amino acid sequence of the recombinant protein is shown as SEQ ID NO: 2. The discovery of the Ferritin L gene of Hefang Crucian Carp not only enriches the gene library of Hefang Crucian Carp, but also can be used to prepare recombinant proteins. Experiments have shown that the recombinant protein can inhibit the growth activity of Aeromonas hydrophila, providing a new practical basis for the physiological immunity research of Hefang Crucian Carp. The present invention also discloses a preparation method of the Ferritin L recombinant protein of Hefang Crucian Carp, the application of the Ferritin L recombinant protein of Hefang Crucian Carp in the fields of inhibiting the growth of Aeromonas hydrophila, immune preparations or feed additives for aquatic animals, and primers for amplifying the Ferritin L gene of Hefang Crucian Carp.

The application discloses application of an NbFKBP13 gene in resisting tomato yellow leaf curl virus and belongs to the technical field of plant disease prevention and treatment. Through genetic engineering technology, the application obtains a stably heritable overexpression NbFKBP13 transgenic N.benthamiana. Phenotype observation finds that the NbFKBP13 gene has no significant influence on the growth and development of the N.benthamiana. Artificial inoculation of TYLCV finds that the NbFKBP13 gene can significantly enhance the resistance of the N.benthamiana to the TYLCV after overexpression. The above research provides an important gene bank and new germplasm resource for virus disease resistance breeding.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to a soybean TFL1a gene and a knockout method and application thereof. The soybean TFL1a gene is edited through a CRISPR / Cas9 technology, a homozygous mutation experience is constructed to prove the function of the soybean TFL1a gene, and it is found that the gene regulates the number of soybean main stem nodes, the number of mutant strain nodes is increased, and the yield is increased. The method has the advantages that on the aspect of gene function analysis, the core function of the gene is determined, a plant type regulation gene pool is supplemented, and functional genomics research is deepened; in the aspect of high-quality germplasm innovation, the mutant breaks through the limitation of traditional breeding and provides a high-quality material for variety improvement; in industrial application, molecular targets and technical paths for plant type improvement and yield increase are provided, the number of sections can be directionally optimized, benefits can be improved, reference is provided for molecular design breeding, and the method is of great significance in germplasm creation, plant type change and yield increase.

The present invention discloses a method for geological disaster early warning based on multi-source information data, comprising the following steps: S1. Constructing a digital rock gene library based on multi-source information data; S2. Determining rock standard detection items and establishing a rock basic information prediction model; S3. Establishing a geological disaster monitoring and early warning model; S4. When carrying out early warning projects, collecting rock samples from the warning area, conducting tests, and substituting the test results into the rock basic information prediction model to obtain the rock basic information of the rock samples; S5. Substituting the information into the geological disaster monitoring and early warning model, analyzing and obtaining early warning dynamic monitoring indicators and early warning thresholds; S6. Performing real-time dynamic monitoring of the early warning dynamic monitoring indicators. The early warning technology of this application uses multiple information source data, which reduces prediction deviation compared to the existing technology that uses a single monitoring source data. The early warning model fully considers the role of rock mechanical properties in geological disasters and has higher accuracy.

The application provides a local livestock and poultry traceability method based on SNP markers, relates to the field of breed traceability, and comprises traceability gene library construction, kinship determination, traceability identification based on genomic data, and traceability identification process and result determination; wherein, the traceability gene library construction comprises source gene library sample collection, sample collection and information recording, gene analysis and data generation, and traceability gene library establishment; the kinship determination comprises repeated individual identification and parent-offspring identification; the traceability identification based on genomic data comprises repeated individual identification based on genomic information and parent-offspring relationship identification based on genomic information; the traceability identification process and result determination comprises sample receiving and quality control, data alignment analysis, and hierarchical result determination. The method realizes accurate traceability of livestock and poultry meat products by obtaining SNP marker information through genomics technology and realizing accurate traceability of livestock and poultry meat products by using kinship analysis, and effectively prevents false and inferior phenomena.

The present invention belongs to the fields of crop science and agricultural molecular biology, and specifically relates to BAK the application of a BAK gene in enhancing the waterlogging tolerance of rapeseed. The BnaA04.BAK7 gene is BnaA04.BAK7 gene; the accession number of the BAK gene in the gene bank is BnaA04g0150000WE. By disrupting the BAK expression of the BAK gene, it is found that after the expression of the BAK gene is disrupted, the waterlogging tolerance of Brassica napus under waterlogging stress is weakened, and the present invention for the first time discloses that the deletion of the BAK gene function will lead to slow hypocotyl growth and reduced chlorophyll content of Brassica napus under waterlogging stress, providing new gene resources and theoretical guidance for the research and application of rapeseed waterlogging tolerance.

The present application relates to the field of plant biotechnology, and particularly relates to application of Eucalyptus grandis EgrDof5 gene in poplar, the application comprises at least one of the following: a, improving the biomass of poplar; b, improving the photosynthetic performance of poplar; c, regulating the secondary growth of poplar; the nucleotide sequence of the Eucalyptus grandis EgrDof5 gene is shown as SEQ ID No. 1. The present application can improve the biomass of poplar, improve the photosynthetic performance of poplar and regulate the secondary growth of poplar by transferring the Eucalyptus grandis EgrDof5 gene into the genome of poplar, which provides a new method for genetic improvement of poplar, helps to enrich the gene library of poplar and provides more choices for future forest breeding.

The embodiment of the invention provides a humanoid robot control method based on an attention mechanism and related equipment, and belongs to the technical field of robot control. The method comprises the following steps: acquiring a task instruction of the humanoid robot and current environment sensing data; fusing the dynamic attention codes with the current state information of the humanoid robot to generate state input of a reinforcement learning model; based on the state input, outputting an action strategy of the robot through a pre-trained reinforcement learning model, and controlling the humanoid robot to execute movement or task operation; if it is detected that the humanoid robot is in the task execution process, environment feedback and execution result data are collected in real time, online optimization is conducted on the reinforcement learning model based on the environment feedback and execution result data, and if it is detected that the humanoid robot completes the task, key experience information of task execution is extracted, coded into a gene segment and stored in a group experience gene pool. According to the embodiment of the invention, the humanoid robot can quickly make a robust decision in an unknown environment.