NGF gene as molecular marker of sheep yeaning traits and application thereof

A molecular marker and sheep technology, which is applied in recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of low selection accuracy, long cycle, and inability to carry out early breeding

Active Publication Date: 2016-12-28
甘肃润牧生物工程有限责任公司
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the defects of the prior art, to provide a molecular assisted breeding marker to increase the lambing size of sheep, and to obtain a molecule related to the lambing traits of sheep by searching for the SNP site of the NGF gene. In order to overcome the technical defects of

Method used

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  • NGF gene as molecular marker of sheep yeaning traits and application thereof
  • NGF gene as molecular marker of sheep yeaning traits and application thereof
  • NGF gene as molecular marker of sheep yeaning traits and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] 1. Blood sample collection

[0031] Peripheral blood samples were collected from sheep with first-born lambing records (breeds Hu sheep and Small-tailed Han sheep), and the blood samples were collected from Jinchang Zhongtian Sheep Industry Co., Ltd., Gansu Province.

[0032] 2. Genomic DNA extraction

[0033] Specific steps are as follows:

[0034] (1) Take 1 mL of collected whole blood and add an equal volume of PBS buffer, and shake gently for 10 minutes;

[0035] (2) After centrifugation at 12000g for 10min at 4°C, discard the supernatant;

[0036] (3) Repeat step (1) and step (2) until the supernatant is transparent and the precipitate turns white;

[0037] (4) Add 700 μL of DNA extraction solution (1M Tris-Cl (pH=8.0) 25mL, 0.5M EDTA (pH=8.0) 100mL, 10% SDS 50mL, 2M NaCl 25mL, set the volume to 500mL, and perform high-pressure Sterilization), suspend the precipitate, and put it in a water bath at 37°C for 1h;

[0038] (5) Add 3 μL proteinase K (20mg / mL) solut...

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Abstract

The invention belongs to the technical field of a livestock molecular marker, and more specifically relates to NGF gene as the molecular marker of sheep yeaning traits and an application thereof. The molecular marker is obtained by NGF gene (Gene Bank ID: 101104540) through cloning, a nucleotide sequence of the molecular marker is shown as a sequence table SEQ ID NO:1, a base mutation of G greater than A is existed at 689th bp of the sequence table SEQ ID NO:1, The SfiI-RFLP digestion polymorphism is generated due to mutation. In the sheep, the bit GG genotype sheep yeaning number is more than the AA genotype sheep yeaning number by 0.58. The invention also discloses a primer pair used for amplifying a NGF gene part DNA sequence and a molecule-labeled detection method, and provides novel labeling resource for marker auxiliary selection of the sheep yeaning traits.

Description

technical field [0001] The invention belongs to the technical field of screening and application of sheep molecular markers, and in particular relates to NGF gene as a molecular marker for lambing traits of sheep and its application. The molecular marker of the present invention is cloned from NGF gene. Background technique [0002] The selection and breeding of breeding sheep is a key link in the breeding and production of breeding sheep in modern sheep farms. Among the major technological factors affecting livestock production efficiency, the contribution rate of genetic breeding and breeding technology accounts for 50%, so improving the fecundity of sheep flocks will help improve production efficiency. First, in the cost of raising sheep in the house, the cost of raising ewes accounts for more than 60% of the total cost of raising, and improving the reproductive performance of ewes can significantly improve the economic benefits of producers. Second, through the selecti...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 李万宏翁秀秀乐祥鹏张军霞王维民李发弟
Owner 甘肃润牧生物工程有限责任公司
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