131results about How to "Overcoming activity" patented technology

The invention provides a preparation method of a nickel-cobalt layered bimetallic hydroxide/graphene electrocatalyst, which belongs to the technical field of electrocatalysts. The method disclosed bythe invention comprises the following steps: graphene and cobalt nitrate are uniformly dispersed in a methanol solvent by ultrasonic waves, so that an ultrasonic solution is obtained; the ultrasonic solution is added into a methanol solution of dimethylimidazole, is uniformly mixed and then is enabled to stand, and centrifuging, washing and drying are then carried out, so that a ZIF-67/graphene composite material is obtained; the ZIF-67/graphene composite material and nickel salt are mixed and dissolved in a solvent, refluxing is carried out for reaction, centrifugal separation is then carriedout, obtained precipitate is washed with absolute ethyl alcohol, and drying is carried out to obtain a NiCo LDH/G composite material with a nanoscale morphology. The method disclosed by the inventionovercomes the defects of high requirement on equipment, need for high temperature and high pressure and need for an expensive surfactant existing in conventional synthesis methods, and compared withthe prior art, the preparation method has the advantages of simple process, low cost, easy reaction process control and the like, and is suitable for industrial mass production.

The method for preparing probiotics is characterized by that it utilizes the metabolic product characteristics of various strains to select the strain for mixed fermentation, in the culture of mixed strain of probiotics of bifidobacterium and lactic acid bacteria, etc. the fungous fermenting liquor of ganoderma, etc. is added to make co-fermentation, so that it not only can raise number of ermentaing living bacteria, prolong the suvival time of living bacteria, but also can retain all the nutrients of fermenting liquor, reduce product cost and retain health-care effect of said product.

The invention discloses preparation and application of human-stem-cell-secreted bioactive factors and a lysis solution. The process comprises the following steps of: extracting stem cells of the umbilical cord or the placenta tissue of a health human person; performing in-vitro culture and amplification; and preparing high-purity and high-activity stem-cell-secreted bioactive factors and bioactive substances in the stem cell (namely, high-purity stem cell lysis solution) by the modern biological technologies of low-temperature separation, purification and the like. According to the invention, the bioactive factors or the substances do not contain any animal component, and can be developed into a product with a cell biological activity; after acting on the human body, the product can activate the stem cell of the organism and repair or replace damaged, lesion and old cells; and the product has the functions of regulating the cell metabolism function of the organism, enhancing the immunity of the organism, delaying the aging and the like. The human bioactive factors or the substances have great application prospect in the fields of medical science, healthcare and beauty.

The invention belongs to the field of building materials and particularly relates to a non-cement green mortar by using the steel slag as the cementing material and the fine aggregate and a preparation method thereof. The non-cement green mortar comprises the following components in weight part: 30-50 parts of steel slag powders, 50-70 parts of mineral powders, 200-350 parts of steel slag sands, 4-7 parts of activator, 1-4 parts of a water reducing agent, 3-6 parts of a dispersing agent, 0-0.4 parts of a retarder, 0.0002-0.001 part of a defoamer, and 30-40 parts of water. The non-cement green mortar is good in working performance, automatic in leveling effect, adjustable in setting time with the range of 0.5-8 hours, high in early-late strength, and excellent in impermeability and resistance to sulfate attack. The 7d breaking strength of the non-cement green mortar is larger than 8 MPa, and the compression strength thereof is larger than 40 MPa. The 28d breaking strength of the non-cement green mortar is larger than 9 MPa, and the compression strength thereof is larger than 50 MPa. Therefore, the non-cement green mortar can be widely applied to the engineering practice.

The invention belongs to the technical field of organic chemical catalytic synthesis, which relates to a device and a method for performing olefin gaseous phase epoxidation by using industrial hydrogen peroxide. A reactor casing of the device is made of metal materials, the inner wall contacted with the hydrogen peroxide is passivated through acid solution, or the inner wall is coated with hydrogen peroxide inert materials. The processed raw material enters a reactor to perform reaction along with feeding materials of the olefin raw material, diluent gas, auxiliaries and the like, and after reacted materials pass through a separation unit, unreacted olefin raw material, the diluent gas and auxiliaries circulate back to the reactor. The device and the method directly use industrial hydrogen peroxide products, overcome bottlenecks of low efficiency, fast catalytic agent inactivation and poor regeneration repeatability of in-situ hydrogen peroxide preparation, are strong in raw material adaptability, safe and high in efficiency, few in gaseous phase epoxidation reactive by-product, simple in separation and high in catalyst stability.

The invention relates to a catalyst for preparing propylene through C4 olefin cracking, and a preparation method thereof. The problems of low stability and low propylene selectivity of present catalysts for preparing propylene through present C4 olefin cracking are mainly solved. The catalyst comprises 20-90% of ZSM-5 molecular sieve with the shape index of 3-100 (the shape index is defined as a length ratio of a longest edge to a shortest edge of a molecular sieve crystal in a three dimensional direction), 0.05-3% of a transition metal oxide and 18-69% of a binder. The catalyst provided by the invention well solves the problems, and can be used in the industrial production for preparing propylene through C4 olefin cracking.

The invention relates to a chair, in particular to a multifunctional medical care chair. The invention provides a multifunctional medical care chair which can be used for moving arm muscle and massaging the back and the neck and comprises a chair back, a weight lifting device, a neck massaging device and a back massaging device; the weight lifting device comprises a pushing rod, a pulling rope and a heavy article; the neck massaging device comprises a rack, a gear, a cam, a face plate, a massaging block and a spring; the back massaging device comprises a massaging roller and a wave rail. The multifunctional medical care chair overcomes the defects that an existing chair cannot be used for moving the arm muscle and massaging the back and the neck, and achieves the effects of moving the arm muscle and massaging the back and the neck.

The present invention discloses mobile terminal service packaging providing method. It contains SP/CP transmitting developed mobile terminal service package to service downloading platform; service downloading platform modifying service package code and adding packaging function to service package used for to complete status report, application activating and application authentication, completing packaging; service downloading platform dispensing said packaged service package to mobile terminal. Added package function includes package message and triggering statement; wherein package message at least including application unique identification APPID, user unique identification User-ID and service downloading platform URL, said triggering statement used for through API interface obtaining or feeding back relevant message to said service downloading platform.

The invention relates to a cartilage preservation solution. Each 1000ml of the preservation solution comprises 20-30mmol of potassium dihydrogen phosphate, 80mmol of histidine or 15mmol of a histidine salt, 75-95mmol of lactobionic acid, 50-70mmol of sucrose, 0.8-1.2mmol of allopurinol, 45-55g of low molecular dextran-40, 45-55mmol of NaCl, 25-35mmol of KCl, 3-7mmol magnesium sulfate, 70-90U of penicillin, 2-4mmol of reduced glutathione, 3-7mmol of adenosine, 2-5g of trehalose, and the balance deionized water, and the pH value of the cartilage preservation solution is 7.33-7.53. The cartilagepreservation solution is packaged, sealed and preserved at 0-4DEG C. By adopting the preservation solution to preserve cartilage tissues, the death speed of cartilage cells is slowed, the activity ofcartilage fibers is maintained, the performance of the cartilage tissues is kept, and the antibiotic property is increased, so the preservation solution has a good preservation effect and a long preservation time on the cartilage tissues, and is in favor of clinic cartilage tissue transplant operations.

The invention provides a high-dispersion supported copper-based catalyst as well as a preparation method and an application thereof. The catalyst can be represented as Cu/MO-ZrO2, wherein copper nanoparticles are uniformly dispersed on a compound metal oxide carrier, the specific surface area of the catalyst is between 60-110m<2>/g, the weight ratio of Cu is 10-40%, and the grain size of Cu is 5-20nm. Precursor nanoparticles of a compound metal hydroxide catalyst uniformly dispersed in molecular level and adjustable in constitution and structure are synthesized by the method by means of a forced micromixing effect caused by a strong shear force, a centrifugal force and a counter-acting force of an all-backmixing liquid film reactor, and a selective guide effect of a surfactant. The catalyst precursor is roasted and reduced to obtain the high-dispersion supported copper-based catalyst. The catalyst is mainly used for selective catalytic hydrogenation of dimethyl oxalate, and the conversion ratio and the selectivity respectively reach 90-100% and 85-95%. The copper-based catalyst has the characteristics of good stability and simple preparation method.

The invention discloses a preparation method and use of a traditional Chinese medicine asiaticoside carrying core/shell structure nanometer fiber film. The traditional Chinese medicine asiaticoside carrying core/shell structure nanometer fiber film is prepared from a lactic acid/glycollic acid copolymer (PLGA) as a shell and polycaprolactone (PCL) as an inner core through a coaxial electrospinning method. The preparation method has the advantages of use of easily available raw materials, operation easiness, good controllability and large scale industrial production easiness. The traditional Chinese medicine asiaticoside carrying core/shell structure nanometer fiber film can be used for wound dressing and has the advantages of controllable drug loading amount, high drug utilization rate and excellent wound healing promoting effect.

The present invention discloses a Qimei black tea processing technology, which comprises raw material selection, withering, rolling, fermentation, shaping, drying, blending matching and other steps. The prepared Qimei black tea has characteristics of slim shape, dark and moisturizing color, and jinhao expose, provides characteristics of red and bright tea soup, enticing aroma emitting, and high and lasting aroma after infusion, and further has characteristics of typical keemun aroma and sweet aroma, fresh and mellow taste, sweet and mellow aftertaste, and red and bright tea residue. In addition, with the processing technology, the defects of bitter taste, thick-black color, boring, no fresh feeling, and low and dull aroma of the previous keemun black tea are overcome, the pursuit of people on the high quality keemun black tea is completely met, and the market prospects are broad.

A method for synthesizing a TS-1 molecular sieve comprises the steps that: (1) the aqueous solution of solid silica gel particles, titanium source and organo-alkali compound is mixed and treated with hydro-thermal treatment at 80-180 DEG C for 3-96 hours, wherein, the molar ratio of SiO2: TiO2: organo-alkali compound: water is 1: 0.001-0.5: 0.01-0.5: 5-100; (2) the aqueous solution of the solid silica gel particles, the titanium source and the organo-alkali compound is mixed until the titanium source is completely hydrolyzed; wherein, the molar ratio of SiO2: TiO2: organo-alkali compound: water is 1: 0.001-0.5: 0.001-0.80: 5-150; (3) the products obtained from step (1) and step (2) are mixed; and crystallization reaction is carried out by a conventional method to recover the product; wherein, the product of step (1) accounts for 10-80 percent. The method of the invention can synthesize the TS-1 molecular sieve with certain granularity, and the synthesized molecular sieve has low content of non-skeleton titanium and has high activity, stability and selectivity when being used in oxidation reaction.

The invention provides a preparation method of 2, 3, 3, 3-tetrafluoropropene, which comprises the following steps: taking monochlorodifluoromethane (R22) and methane chloride (methyl chloride) as raw materials and carrying out thermal cracking reaction at a high temperature of 600-1000 DEG C under the condition that N2 exits to prepare the 2, 3, 3, 3-tetrafluoropropene, wherein the volume ratio of the monochlorodifluoromethane to the methane chloride is 1-3:1, and the retention time of a reactant is 0.01-0.2 seconds. The reaction raw material used in the preparation method is easy to obtain, the defect of high cost of the used raw material in the prior art is overcome, the production cost is greatly reduced, and the reaction process is simple. The prepared 2, 3, 3, 3-tetrafluoropropene has the advantages of zero ODP and low GWP and can be used as a substitute of an automotive air-conditioning refrigerant.

The invention provides a carbon-loaded surface platinum-enriched platinum-nickel intermetallic compound which is prepared by the following method: adding carbon black, sodium glutamate and sodium citrate into an ethylene glycol solution containing a platinum precursor and a nickel precursor, and generating a carbon-loaded platinum-nickel disordered alloy through a solvothermal reaction; then carrying out heat treatment of the carbon-loaded platinum-nickel disordered alloy in a reducing atmosphere, and transforming into a carbon-loaded platinum-nickel ordered intermetallic compound; and finally, dispersing the carbon-loaded platinum-nickel ordered intermetallic compound in water, carrying out surface platinum enriching through dropping a platinum precursor aqueous solution, and generating the carbon-loaded surface platinum-enriched platinum-nickel intermetallic compound. The surface platinum enriching treatment is carried out based on the carbon-loaded platinum-nickel ordered intermetallic compound, the activity and the stability of a catalyst are improved while the amount of platinum metal is relatively lowered, and the problems that a traditional platinum-carbon catalyst has large amount of precious metal platinum, low catalytic activity and poor stability and the like are overcome. The invention also discloses a preparation method and application of the carbon-loaded surface platinum-enriched platinum-nickel intermetallic compound.

The invention provides an optimized high-activity human lysozyme gene as well as expression vectors thereof. The gene sequence is optimized according to the codon bias of Pichia pastoris and the human lysozyme gene is artificially synthesized; recombinant plasmids pUC18-T-HZ and pPICZ(alpha)A-HZ are constructed; the recombinant plasmid pPICZ(alpha)A-HZ is linearized and then transfected to Pichiapastoris, and positive transformants are obtained by screening. The study results show that the recombinant Pichia pastoris can secrete and express high-activity human lysozyme, and the expression product has significant bacteriostatic activity. According to the invention, the Pichia pastoris expression system is adopted to produce the human lysozyme with high expression level and high activity, so as to overcome the problems in the prior art, such as high cost, low expression level and low activity. The production and the promotion and application of the high-activity and low-cost lysozyme can bring considerable economic and social benefits for the development of feed and animal husbandry industries in China and huge ecological benefit as well.

The invention relates to an electrical filter board which is an anode-cathode integrated filter board prepared from an anode electrocatalysis material, a cathode electrocatalysis material and a microelectrolysis material or a plate-shaped anode or cathode filter board prepared from the anode or cathode electrocatalysis material, the microelectrolysis material and a conductive material. When the electrical filter board is cooperatively used with a novel parallel plate-type ultrasonic reactor in treatment of highly concentrated organic wastewater, a substantial synergistic effect is obtained; organic compounds in the wastewater are degraded into micromolecular compounds through reactions like electrocatalysis, microelectrolysis and electric flocculation initiated by the electrical filter board and through strongly oxidizing .OH generated by ultrasonic treatment, so biodegradability of the wastewater is improved; moreover, a mechanical shearing effect generated by ultrasonic treatment can effectively clean oxides on the electrical filter board, so disadvantages that a traditional electrocatalysis material easily undergoes inactivation and passivation and a traditional microelectrolysis material easily undergoes hardening are overcome, electrochemical reactivity is guaranteed, and treating efficiency is improved.

The invention particularly relates to an electrochemical biosensor capable of directly detecting an organic phosphorus pesticide in a water phase. The sensor comprises a glassy carbon electrode, a nano gold particle layer and a ZrO2 nano particle layer. A production method of the sensor comprises the following steps of firstly electro-plating a layer of nano gold particles on the surface of the glassy carbon electrode, and then electro-plating a layer of zirconium dioxide nano particles on the nano gold particles. After the produced sensor fully adsorbs in a prepared organic phosphorus solution, and quantitative detection of the organic phosphorus is realized through the dissolution peak current detected by an electrochemical workstation. The sensor takes full advantage of special performances of the nano gold particles and the ZrO2 nano particles in structure, is low in production cost, high in sensitivity, wide in linear detection range, good in reproducibility, small in nonspecific adsorption, and simple and fast in detection process, and has great application potential in food safety monitoring field.

The invention provides a method for preparing an Ag/AgCl/BiMg2VO6 composite photocatalyst with a visible light photocatalytic activity. The method is characterized in by comprising the following steps: obtaining a BiMg2VO6 base material from the raw materials: bismuth nitrate pentahydrate, magnesium nitrate hexahydrate, and ammonium metavanadate, by a sol-gel method; adding BiMg2VO6 into deionized water and then ultrasonically treating the mixed solution; then, slowly dropwise adding 0.1 mol/L silver nitrate solution at a proportion of 1 to 3 mmol/g of Ag/BiMg2VO6 and then ultrasonically treating the mixed solution for 10 min, and stirring the mixed solution for 20 min at room temperature in the dark; slowly adding 0.1 mol/L hydrochloric acid solution into the mixed solution at a mole ratio of 1:1 or 1:2 of H ions in the silver nitrate solution to H ions in the hydrochloric acid solution, ultrasonically treating and stirring the mixed solution to generate a AgCl/BiMg2VO6 composite photocatalyst; and optically reducing the AgCl/BiMg2VO6 composite photocatalyst at an ultraviolet lamp of 20 W to obtain the Ag/AgCl/BiMg2VO6 composite photocatalyst. The composite photocatalyst prepared by the method has a high photocatalytic activity in visible light and a simple preparation process.

The invention provides a preparation method of modified biochar, the modified biochar, a composite material and a soil repairing agent, and relates to the technical field of biochar. The preparation method of the biochar comprises the following steps: mixing peanut shell powder with a graphene oxide suspension to obtain peanut shell powder impregnated with the graphene oxide suspension; mixing theobtained peanut shell powder impregnated with the graphene oxide suspension with the nano-SiO2 to obtain a mixed material; mixing the obtained mixed material with water to obtain a mixture; carbonizing the mixture to obtain hydrated carbon, and drying the obtained hydrated carbon to obtain the modified biochar. The modified biochar provided by the invention has porosity, large specific surface area and good adsorption capacity, and can effectively reduce the activity of soil heavy metals in the soil.

The invention provides boulardii active dry yeasts, wherein the total amount of cells of the dry yeasts is 30-75 billions/g, the rate of living cells is 50-88%, and the water content is 4-6%. A production method of the boulardii active dry yeasts comprises the following steps of: (1) culturing strains: inoculating and culturing Saccharomyces boulardii strains; (2) culturing seeds in a fermentation tank; (3) ventilating air flows into the fermentation tank for culture, after 20-28 hours of fermentation, adjusting the pH value of fermentation liquor to be 6.1-6.4, performing cell decolorization to ensure that the color of the product is lighter, and in 1.5-3 hours after the pH value is adjusted to be 6.1-6.4, raising the temperature of the fermentation liquor to 30-35 DEG C to perform fermentation, and adding sodium chloride at the same time; (4) separating; (5) adding an emulsifying agent and vegetable oil, and then granulating and molding; and (6) drying to obtain yeast particles.

The invention relates to an application of a WAP (whey acidic protein) gene in transgenosis of a fruit fly. According to the application, the WAP gene in Fenneropenaeus chinensis is constructed in the fruit fly and is expressed in the fruit flies. The transgenic fruit fly prepared by utilizing the application has an antibacterial effect.

The invention discloses a method for preparing oxidized graphene. The method includes steps of adding graphite powder into concentrated sulfuric acid at the low temperature and carrying out ultrasonic treatment under a stirring condition; adding sulfuric acid solution into obtained mixed solution, continuously filling the mixed solution with gas containing ozone, then gradually increasing the temperature until moderate-temperature environments are created and carrying out constant-temperature stirring reaction; continuously filling reaction products with gas containing ozone, starting to add strong oxidizing agent solution into the reaction products, gradually increasing the temperature until high-temperature environments are created and carrying out constant-temperature stirring reaction; carrying out high-temperature oxidation exfoliation to obtain mixed liquid, carrying out centrifugal treatment on the mixed liquid, separating upper-layer clear liquid from lower-layer suspension, deeply exfoliating the lower-layer suspension to obtain dispersion liquid containing the oxidized graphene, or carrying out deep exfoliation, centrifugal separation, washing and drying treatment on the lower-layer suspension to obtain oxidized graphene powder. The method has the advantages that the oxidized graphene prepared by the aid of the method is high in purity, good in safety and large in laminar structure distance, waste acid can be recycled, and preparation procedures are green and environmentally friendly.

The invention belongs to the technical field of food safety detection, and discloses a method for detecting veterinary drug residue of an animal-derived product. The method comprises the following steps: analyzing an extracting solvent, an extracting method and a purifying technology suitable for a sample to be detected, and extracting and purifying components to be detected in the sample; separating 63 analytes under a liquid chromatography separation condition, and performing baseline separation on 6 groups of isomers; simultaneously detecting sulfonamides, sulfonamide synergists, quinolones, beta-receptor agonists, tetracyclines, macrolides, quinolines, nitroimidazoles, cephalosporins and penicillins, altogether 63 veterinary drugs in 10 categories, through selection and use of an MS/MSdetector, an ESI ion source, a schedul MRM mode, three-ion confirmation and an internal/external standard quantifying technology. The method provided by the invention is applied to detection of 10 categories, altogether 63 kinds, of veterinary drugs, such as sulfonamides in a daily animal product; through improvement on the working efficiency and reduction in the detection cost, a food safety emergency can be quickly handled, and social and economic benefits are brought.

The invention provides a composite oxygen carrier as well as a preparation method and application thereof to the gasification of a solid fuel, and belongs to the technical field of energy chemical industry. The composite oxygen carrier is olivine loaded by copper oxide; the copper oxide accounts for 5 to 30 percent of the mass of the olivine. According to the method, an oxygen carrier is utilized for circularly transferring oxygen and heat; an absorbent is utilized for circularly removing carbon dioxide and carrying out desulfurization; meanwhile, the gasification of a raw material and the purification of a produced gas are realized. In addition, a calcination reactor and an air reactor are connected in series; the heat generated by the oxidation regeneration of the oxygen carrier/the combustion of a residual fuel can be used for realizing the calcination regeneration of the absorbent. The made oxygen carrier has the advantages of being low in cost, high in activity, good in abrasion resistance, high in cyclical stability and high in anti-sintering capacity; the gasification of the raw material and the purification of the produced gas are realized at the same time through a double circulation device; the structure is simple; the control is easy; the utilization rate of energy is high; the quality of the produced gas is good; the environmental pollution is little.

The invention discloses a lysozyme mutant with improved specific activity, belonging to the technical field of bioengineering. The invention discloses the human lysozyme mutant which is obtained by adding hydrophobic oligopeptide Val-Ile-Pro-Leu-Phe to the C end of human lysozyme through genetic engineering. The specific activity of the human lysozyme mutant is improved by 126%. The invention alsodiscloses a recombinant plasmid containing a mutant human lysozyme gene, and a recombinant genetically-engineered pichia pastoris strain which is obtained by transforming pichia pastoris with the recombinant plasmid and capable of efficiently expressing the human lysozyme mutant. The obtained human lysozyme mutant has the characteristics of high specific activity and simple preparation, has potential clinical application value, and also has wide application in feed and food industries.

The invention discloses a tissue engineering material with a biologically active surface layer and a preparation method thereof. The tissue engineering material comprises a biodegradable high polymer material matrix and a dopamine layer adsorbed on the surface of the matrix, wherein the dopamine layer is connected with a biologically active polymer. The preparation method comprises the following steps: providing the biodegradable high polymer material matrix with a thoroughly wetted surface; providing a buffer system with dissolved dopamine and the biologically active polymer; and fully soaking the matrix in the buffer system, carrying out incubation at 30 to 40 DEG C for 1 to 5 h, then taking the matrix out and subjecting the matrix to air drying so as to obtain the tissue engineering material with the biologically active surface layer. The tissue engineering material has high mechanical properties and controllable degradation performance and good biological activity and is beneficial for adherence and propagation of cells; and the preparation method is simple, easy to operate, low in cost and favorable for large-scale implementation.

The invention provides a nano enzyme with a nanometer cage core of a hollow gold-platinum alloy and a shell of a porous silicon dioxide, a preparation method and an application thereof, and belongs tothe field of nanometer materials. The preparation method comprises the following steps: etching a gold core platinum shell nanostructure through potassium hexachloroplatinate to obtain a nanocage ofhollow gold-platinum alloy; coating silicon dioxide on the surface of the nanocage through tetraethyl silicate; and obtaining the nano enzyme taking the nanometer cage, in a round rod shape, of the hollow gold-platinum alloy as a core, and a silicon dioxide shell as the periphery of the core. The length of the core is 80 to 100 nm, and the diameter is 20 to 25 nm. The shell is provided with a channel of 3 to 5 nm. The nano enzyme is negatively charged. The antigen can be bound by an attractive effect of positive and negative electric charges to form a nano enzyme label antigen complex, which is used for performing enzyme linked immunoassay. According to the nano enzyme with the nanometer cage core of the hollow gold-platinum alloy and the shell of the porous silicon dioxide, the preparation method and the application thereof, the cost of a reagent of the natural enzyme label antigen complex is reduced, the working environment of enzyme linked immunodetection is extended, the defects that the natural enzyme is easy to deactivate and denature, etc., are overcame, and the nano enzyme can be widely applied to various enzyme linked immunodetection.

The invention provides a Brazilian green propolis quality control method. The Brazilian green propolis quality control method is implemented through the following steps that a test propolis sample is ultrasonically extracted with ethyl alcohol, filtered and detected through high performance liquid chromatography, the content of six evaluation index ingredients is calculated according to a standard curve, and whether the quality of Brazilian green propolis is high or low is evaluated through the content of the six ingredients, that is to say, if the total sum of the content of the six ingredients is larger than 10%, the Brazilian green propolis is high-quality propolis, and if the total sum of the content of the six ingredients is smaller than 6%, the Brazilian green propolis is low-quality propolis. According to the method, the chemical ingredients of the Brazilian green propolis are combined with the antioxidant activity, the antioxidant material basis of the Brazilian green propolis is illustrated, the defect that some main ingredients do not achieve the high pharmacological activity is avoided, and the antioxidant activity of the Brazilian green propolis can be reflected more truly; the design is reasonable, the irrationality that in an existing method, total flavonoid is taken as the quality control index of the Brazilian green propolis is overcome, the defect that content detection of multiple flavones or phenolic acids has no correlation with the activity is overcome, and a novel method is provided for Brazilian green propolis quality control.

A process for renaturating and purifying the inclusion body protein includes such steps as dissolving the inclusion body protein in acidic/alkaline flowing phase or other solution, loading it in the ion-exchange chromatographic column, reversible adsorbing it by the chromatographic medium, and modifier concentration gradient elution. Its advantages are high concentration and activity of recovered protein and high purity.