Organic phosphorus electrochemical biosensor based on adsorption of nano particles

A biosensor and nanoparticle technology, which is applied in the field of analysis and detection, can solve the problems of expensive gold electrodes, achieve good regeneration, reduce construction costs, and have small non-specific adsorption effects

Inactive Publication Date: 2014-04-23
湖南省茶叶研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, considering the high price of gold electrodes, in order to reduce the manufacturing cost of the sensor, further improve the reaction performance of the electrode, and expand the range of monitoring targets, further research is needed to improve the practical performance of the sensor.

Method used

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  • Organic phosphorus electrochemical biosensor based on adsorption of nano particles
  • Organic phosphorus electrochemical biosensor based on adsorption of nano particles
  • Organic phosphorus electrochemical biosensor based on adsorption of nano particles

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The glassy carbon electrodes are sequentially used with 0.3 μm and 0.05 μm A1 2 o 3 The powder was polished to a mirror surface, and then ultrasonically cleaned in 95% ethanol and distilled water for 4 min, and then rinsed with secondary water and blown dry with nitrogen. Place the cleaned electrode in 4 mmol / L chloroauric acid solution, add 0.1 mol / L KNO 3 To support the electrolyte, CHI760b electrochemical workstation was used to electrodeposit nano-gold particles. The electrochemical reaction cell was a three-electrode system, the glassy carbon electrode was used as the working electrode, the saturated calomel electrode was used as the reference electrode, and the platinum electrode was used as the counter electrode. Electrodeposited at 1.0 V for 150 s, a layer of gold nanoparticles was electrodeposited on the glassy carbon electrode.

[0031] Rinse the glassy carbon electrode on which gold nanoparticles have been deposited with 95% ethanol and water in sequence,...

Embodiment 2

[0043] Electrodeposit a layer of nano-gold particles on the surface of the glassy carbon electrode in the same way as in case 1, and then electrodeposit a layer of zirconia nanoparticles on the nano-gold particles to make ZrO 2 Organophosphorus electrochemical biosensor with particle layer / nano-gold / glassy carbon electrode structure.

[0044] The above organophosphorus sensor can be directly used for the detection of paraoxon in water phase. The organophosphorus sensor was soaked in the paraoxon solution, stirred gently for 5 min for adsorption, and then the electrochemical detection of the stripping peak current was performed on a CHI760b electrochemical workstation. First take the paraoxon standard solution concentration of known concentration as the abscissa, and the corresponding square wave stripping voltammetry peak current as the ordinate to draw a graph to obtain the working curve of the sensor for paraoxon. The sensor SWV stripping peak current has a linear relations...

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Abstract

The invention particularly relates to an electrochemical biosensor capable of directly detecting an organic phosphorus pesticide in a water phase. The sensor comprises a glassy carbon electrode, a nano gold particle layer and a ZrO2 nano particle layer. A production method of the sensor comprises the following steps of firstly electro-plating a layer of nano gold particles on the surface of the glassy carbon electrode, and then electro-plating a layer of zirconium dioxide nano particles on the nano gold particles. After the produced sensor fully adsorbs in a prepared organic phosphorus solution, and quantitative detection of the organic phosphorus is realized through the dissolution peak current detected by an electrochemical workstation. The sensor takes full advantage of special performances of the nano gold particles and the ZrO2 nano particles in structure, is low in production cost, high in sensitivity, wide in linear detection range, good in reproducibility, small in nonspecific adsorption, and simple and fast in detection process, and has great application potential in food safety monitoring field.

Description

technical field [0001] The invention specifically relates to a method for preparing an organophosphorus electrochemical biosensor for rapid detection of organophosphorus pesticides in an aqueous environment, and belongs to the technical field of analysis and detection. Background technique [0002] Due to the unreasonable or excessive use of chemical pesticides in the process of crop pest control, some agricultural products have high pesticide residues, which not only affects human health, but also destroys the agricultural ecological environment. [0003] The traditional analysis methods of organophosphorus pesticides include gas chromatography, thin layer chromatography, high performance liquid chromatography and various spectroscopic techniques. These methods have relatively high selectivity, sensitivity and accuracy, but require expensive instruments and equipment , the sample pretreatment method is complicated, and the required analysis cycle is long, which has certain ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48G01N27/30
Inventor 刘淑娟钟兴刚李彦郑红发黄怀生赵熙粟本文
Owner 湖南省茶叶研究所
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