This invention discloses a
nanocomposite material and its preparation method, a nanoenzyme-bioenzyme
cascade sensor and its application, relating to the field of
pesticide detection technology. The technical solution of this invention introduces flexible two-dimensional
graphitic carbon nitride nanosheets as a soft template and spatial confinement agent for in-situ growth, effectively suppressing the
excessive growth of PCN-222(Fe) crystals during the solvothermal process, successfully controlling its size from the
micrometer scale to the submicrometer scale, resulting in higher catalytic activity. The nanoenzyme-bioenzyme
cascade sensor constructed based on this
composite material achieves broad-spectrum, ultrasensitive detection of pesticides such as
glyphosate,
dimethoate, and
paraoxon. This sensor has a low
detection limit in the pM-nM range, a wide
linear range, and exhibits excellent
recovery and precision in complex real-world samples, strong anti-interference ability, and high practicality.