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Hydroxyl-containing microporous organic polymer organophosphorus pesticide biosensor and preparation method

An organophosphorus pesticide and biosensor technology, applied in instruments, scientific instruments, measuring devices, etc., can solve the problems of on-line and rapid analysis and monitoring of environmental and food pollution, complex sample pretreatment, and expensive instruments. Sensitivity and stability, short preparation time, low cost effect

Inactive Publication Date: 2020-01-10
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the detection methods of organophosphorus pesticides mainly include high performance liquid chromatography, gas chromatography-mass spectrometry, fluorescence and surface plasmon resonance, etc. Although these methods have obtained high sensitivity, most of the instruments they use are expensive and Large size, long test period and complex sample pretreatment limit the on-site and rapid analysis and monitoring of environmental and food contamination

Method used

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  • Hydroxyl-containing microporous organic polymer organophosphorus pesticide biosensor and preparation method
  • Hydroxyl-containing microporous organic polymer organophosphorus pesticide biosensor and preparation method
  • Hydroxyl-containing microporous organic polymer organophosphorus pesticide biosensor and preparation method

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Effect test

Embodiment 1

[0057] This embodiment provides a kind of preparation of Hy-MOP, and this method comprises the following steps:

[0058] Step 1. Dissolve phloroglucinol and terephthalaldehyde in 1,4-dioxane respectively, then add hydrochloric acid, react in microwave for 2 hours at 250W, and naturally cool the obtained brown product to room temperature;

[0059] Step 2, placing the brown product obtained in step 1 in tetrahydrofuran, stirring, suction filtering after the stirring, and washing the filter cake to obtain a brown solid;

[0060] In step 3, the brown solid obtained in step 2 is dried to obtain the Hy-MOP product.

[0061] As a specific example of this embodiment, the preparation of the Hy-MOP includes the following steps:

[0062] In step 1, 1.0 g of phloroglucinol and 0.8 g of terephthalaldehyde were dissolved in 10 mL of 1,4-dioxane, respectively, and put into a 25 mL three-necked flask. Subsequently, 2 mL of hydrochloric acid (mass concentration: 37%) was added, and microwave...

Embodiment 2

[0066] This embodiment provides a hydroxyl group-containing microporous organic polymer organophosphorus pesticide biosensor, including a carbon paste electrode, an acetylcholinesterase layer, and a Nafion layer. The modified layers on the surface of the carbon paste electrode are Hy - MOP layer, acetylcholinesterase layer and Nafion layer.

[0067] Wherein, Hy-MOP adopts the Hy-MOP prepared by the preparation method of Example 1.

[0068] The preparation method of the hydroxyl group-containing microporous organic polymer organophosphorus pesticide biosensor of this embodiment, such as figure 1 As shown, the method includes the following steps:

[0069] Step 1, ultrasonically dispersing Hy-MOP powder in a solvent to form a Hy-MOP suspension; the concentration of the Hy-MOP suspension is 10 mg / mL;

[0070] In step 2, pipette 6 μL of the Hy-MOP suspension prepared in step 1 and drop-coat the surface of the carbon paste electrode, and place it at room temperature to dry to obta...

Embodiment 3

[0087] The electrochemical impedance of different modified electrodes in embodiment 2: figure 2 It is the EIS diagram of different modified electrodes. It can be seen from the figure that the arc radius corresponding to the high-frequency region in the impedance diagram of CPE is the largest, indicating that its electrons are difficult to transfer on the surface of the CPE; the impedance diagram of the Hy-MOP / CPE modified electrode is high The radius of the arc in the high-frequency region is greatly reduced, indicating that the Hy-MOP material has good electron conductivity; when the insulator AChE is further modified on the surface of the Hy-MOP / CPE electrode, it is found that the corresponding impedance arc radius has increased. Large, indicating that AChE was successfully immobilized on the surface of the Hy-MOP / CPE electrode.

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Abstract

The invention provides a hydroxyl-containing microporous organic polymer organophosphorus pesticide biosensor and a preparation method thereof. The biosensor comprises a carbon paste electrode, an acetylcholin esterase layer and a Nafion layer, and modified layers on the surface of the carbon paste electrode are sequentially a Hy-MOP layer, an acetylcholin esterase layer and a Nafion layer from inside to outside. The biosensor provided by the invention has better enzyme immobilization capability and good biocompatibility and has higher sensitivity and stability. Hy-MOP suspension, AChE solution and Nafion solution are sequentially fixed on the carbon paste electrode, so that the organophosphorus pesticide biosensor is constructed. The biosensor provided by the invention can be used for detection on methyl parathion and paraoxon and is low in cost, easy to operate, high in sensitivity and good in selectivity, and linear detection ranges of the methyl parathion and paraoxon are respectively 5.0*10<-13>-1.0*10<-8>g / mL and 1.0*10<-13>-1.0*10<-9>g / mL.

Description

technical field [0001] The invention belongs to the field of organophosphorus pesticides, and relates to a biosensor, in particular to a hydroxyl-containing microporous organic polymer organophosphorus pesticide biosensor and a preparation method. Background technique [0002] Organophosphorus pesticides are often used as herbicides, insecticides and fungicides, and are widely used in agriculture, industry, medicine and other fields due to their advantages of high efficiency, convenient use, small amount, multiple modes of action, and short half-life. As a big country in the production and consumption of pesticides in the world, my country's human health and environmental safety have been seriously threatened due to the abuse of pesticides. Therefore, how to establish an efficient, rapid, and highly sensitive method for monitoring organophosphorus pesticides has become a research hotspot in the fields of environment and food. [0003] At present, the detection methods of or...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277
Inventor 董社英谢强黄贵琦魏文波黄廷林
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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