192 results about "Carbon paste electrode" patented technology

A method for preparing chemical modified electrode of carbon paste Bi membrane includes mixing and grinding carbon powder and modifier as well as hydrophobic organic solvent to be carbon paste, packing prepared carbon paste in electrode tube with copper screw bar in proper size to form carbon paste electrode, placing formed carbon paste electrode into sample solution containing with Bi ion and carrying out constant-potential deposit to obtain chemical modified electrode of carbon paste Bi membrane.

The invention discloses a novel electrochemical DNA sensor constructed by a nanogold and partially-reduced graphene oxide (p-RGO) modified ionic liquid carbon paste electrode (CILE) as a platform prepared by an electrochemical method, and a method of applying the CILE to detect the Listeria feature gene sequence. 1-hexylpyridiniumhexafluorophosphate as a modifier is used for preparing the substrate electrode CILE, gold nanoparticles are deposited on the surface of the substrate electrode CILE and then the p-RGO is prepared by controlling electrochemical reduction conditions; an amino-modified probe ssDNA is fixed on the surface of the electrode by an amido bond covalent bonding method through carboxyl groups remained on the surface of p-RGO to constitute ssDNA / p-RGO / AuNPs / CILE; methylene blue (MB) as an indicator is used for detecting the hybridization reaction after a target sequence is hybridized, and a differential pulse voltammetry (DPV) is used for detecting the Listeria feature gene segment.

The invention discloses a composite film modified DNA sensor. The DNA sensor is characterized in that the sensor comprises a carbon paste electrode; the carbon paste electrode comprises a carbon rod, a Teflon tube, a magnet, and carbon paste; an induction end of the carbon paste electrode is coated with a sensitive substance; the sensitive substance comprises a composite film and a DNA capture probe; magnetic nanoparticles, multi-walled carbon nanoscale tube-gold nanoparticles and chitosan are utilized for modifying orderly the surface of the carbon paste electrode and compose the composite film; and the DNA capture probe is utilized for modifying the surface of the multi-walled carbon nanoscale tube-gold nanoparticles. The invention also discloses a preparation method of the DNA sensor. The preparation method comprises the processing steps of manufacturing a carbon paste electrode, modifying the surface of an induction end of the manufactured carbon paste electrode by a sensitive substance, and the like. The invention further discloses an application of the DNA sensor in detecting a lignin peroxidase (Lip) specific coding gene segment. Because a sensitive substance is utilized for modifying the surface of a carbon paste electrode of the DNA sensor, the DNA sensor has a strong capacity of electron conduction and a high accuracy of detection. The preparation method has the advantages of low cost and simple process.

The invention relates to a preparation method for conductive polymer modification activated carbon electrode materials in a super capacitor, which comprises the following steps: conductive polymer monomers are added into ionic liquid to obtain A solution with the monomer concentration of 0.01-0.5mol / L; the A solution is added into an electrolytic cell deployed with three electrodes; ampere density of 1-10 mA / cm2 is applied to an activated carbon electrode; the electrochemical polymerization is carried out under the protection of nitrogen; after the polymerization is finished, a layer of even conduction polymer film can be obtained on an activated carbon working electrode. The preparation method has the beneficial effects that by using conductive polymer modification activated carbon electrodes, the double layer capacitance of the activated carbon not only can be used, but also the pseudo-capacitance of the conductive polymers can be used for increasing the specific capacitance of the capacitor; compared with a pure activated carbon electrode, the specific capacitance is increased by 2-3 times; and the electrode has high conductivity, fast charge and discharge capability and better circulation stability.

The invention discloses preparation of a three-dimensional graphene manganese dioxide nano-composite modified electrode and a capacitive property test method thereof. An ionic liquid modified electrode is prepared firstly, and then three-dimensional graphene and manganese dioxide nanomaterial are electrodeposited on the surface of the ionic liquid modified carbon paste electrode by utilizing a constant potential method so that a three-dimensional graphene manganese dioxide nano-composite modified carbon paste electrode is obtained. The three-dimensional graphene manganese dioxide nano-composite modified electrode acts as a working electrode, a platinum sheet acts as an auxiliary electrode and a saturated calomel electrode acts as a reference electrode, and capacitive property of three-dimensional graphene manganese dioxide nano-composite material is tested by applying a cyclic voltammetry, an AC impedance method and a constant current charge and discharge method. The three-dimensional graphene manganese dioxide nano-composite modified electrode is simple in preparation method, high in single electrode capacitance and great in cycle performance so as to be suitable for rapidly testing capacitive property of super-capacitor electrode material.

The invention discloses a graphene (GR)-exfoliation hydrotalcite-like compound (ELDH) composite material immobilized protein modified electrode, a production method and an application thereof. The method comprises the following steps: compounding ELDH with negatively-charged exfoliation graphene oxide (GO) nanosheets through electrostatic attraction by using the characteristics of positive charges and large specific surface area of the ELDH, and reducing by using hydrazine hydrate to prepare a GR-ELDH hybrid; and sequentially immobilizing the GR-ELDH, ferrohemoglobin and chitosan on an ionic liquid modified carbon paste electrode through adopting a dispensing technology to produce the graphene-exfoliation hydrotalcite-like compound composite material immobilized protein modified electrode. The obtained modified electrode has monolayer GR nanosheet and ELDH synergistic effects, and the GR nanosheets increase the conductivity of the ELDH and inhibits the aggregation and accumulation of the ELDH; and the ELDH effectively inhibits the afresh stacking of the GR nanosheets, reduces the interlamellar contact resistance and improves the electron transfer rate of the above composite material, and a constructed CTS / GR-ELDH-Hb composite film-based third-generation trichloroacetic acid sensor has the advantages of low detection limit, wide detection range and small Michaelis constant.

The invention relates to a biosensor, particularly relates to a preparation method and catalytic application of a Nafion / horseradish peroxidase / tricobalt tetraoxide-graphene / ionic liquid carbon paste electrode (Nafion / HRP / Co3O4-GR / CILE) and belongs to the technical field of electrochemical analysis and detection. According to the preparation method of the Nafion / HRP / Co3O4-GR / CILE, an ionic liquid carbon paste electrode is used as a substrate electrode, and by virtue of the specific advantages of a Co3O4-GR nano composite material of large surface area and good biocompatibility, HRP is adsorbed on the surface of the material; and furthermore, the material is fixed by a Nafion film to prepare the Nafion / HRP / Co3O4-GR / CILE. HRP in the modified electrode keeps a natural structure and biological activity. Research on electrochemical properties of the modified electrode shows that the modified electrode has good electrochemical catalysis capability on trichloroacetic acid (TCA), and can be used for effectively detecting TCA, and the detection limit is 0.33mmol / L. The preparation method and the catalytic application, provided by the invention, do not cause environmental pollution; the modified electrode has good stability, repeatability and sensitivity, and the preparation process is simple, low in cost and easy to operate, so that the modified electrode has wide social benefits.

The invention discloses a graphene (GR)-hydrotalcite-like compound (LDH)-graphite phase carbon nitride (g-C3N4) composite material immobilized protein modified electrode, a production method and an application thereof. The method comprises the following steps: compounding an exfoliation hydrotalcite-like compound with negatively charged exfoliation graphene oxide nanosheets, mixing the obtained material with melamine, roasting, and adding water to recover in order to prepare a GR-LDH-g-C3N4 hybrid; and adding a GR-LDH-g-C3N4 and Hb mixed solution to an ionic liquid modified carbon paste electrode in a dropwise manner in order to produce the GR-LDH-g-C3N4 composite material immobilized protein modified electrode. The graphene-hydrotalcite-like compound-graphite phase carbon nitride composite material immobilized protein modified electrode fully displays advantages of a gel method and an integrating, merging and layering method, increases the specific surface area, the conductivity and the biocompatibility of the hybrid material, and inhibits the afresh stacking of GR sheets; and a roasting method allows g-C3N4 to grow on the surface of a GR sheet layer, increases the specific surface area of the hybrid material, and improves the electron transfer rate of an electrochemical reaction process. A constructed CTS / GR-LDH-g-C3N4-Hb composite film-based third-generation trichloroacetic acid sensor has the advantages of low detection limit, wide detection range and small Michaelis constant.

The invention provides a preparation method of a modified carbon paste electrode based on a covalent organic framework material and relates to the technical field of electrochemical sensors. The preparation method comprises the following steps: dissolving 1,3,5-tri(4-aminophenylbenzene) and 2,5-dimethoxyterephthalaldehyde into a solvent; reacting under the catalysis of acetic acid to obtain TAPB-DMTP-COF (Tert-Amylperoxybenzoate-Methanol To Propylene-Covalent Organic Framework); then mixing the TAPB-DMTP-COF and graphite powder and grinding; then blending paraffin oil to obtain a mixed material; then pressing the mixed material into a polytetrafluoroethylene pipe and inserting a lead wire to prepare the modified carbon paste electrode based on the covalent organic framework material. According to the preparation method provided by the invention, the electrochemical sensor is constructed to detect lead, and the carbon paste electrode is modified through the TAPB-DMTP-COF so that the current response on the lead is improved.

The invention discloses a production method and an application of a nano-platinum doped / enzyme modified carbon paste electrode. The method is characterized in that a carbon paste electrode is doped with nano-platinum ions, and comprises the following steps: mixing carbon nanotubes with graphite powder with 1-ethyl-3-methylimidazole tetrafluoroborate as an adhesive to prepare a carbon paste electrode, and modifying the carbon paste electrode with methyltransgerase to obtain the nano-platinum doped / enzyme modified carbon paste electrode. Compared with common carbon paste electrodes, the nano-platinum doped / enzyme modified carbon paste electrode has 1-2 times rising conductivity, wide electrochemical window, simple production method, low cost, easy updating of the surface, and small residual current; the electrode can be used to rapidly detect SAM in a sample, so a detection method using the electrode has the advantages of high sensitivity, good selectivity, short response time and less interference, is better than other detection methods, and is a simple, rapid and easy SAM determination method; and an immobilized methyltransgerase electrode sensor made in the invention has the advantages of low cost, simple production process, good specificity and automatic onsite determination realization potential.

The invention provides an encapsulated [VW12]4-cluster metal organic nanotube micropore crystalline state material, and a preparation method and an application thereof and relates to a multi-acidic group micropore crystalline state material, and a preparation method and the application thereof. The invention aims to solve the problems that the synthesis difficulty of the present multi-acidic group metal-organic frame crystalline state material is high and the effect of detecting iodate according to an electrochemical method is poor. The chemical formula of the encapsulated [VW12]4-cluster metal organic nanotube micropore crystalline state material is [Co2(bimb)2VW12O40].[bimb].5H2O. The method comprises the following steps: 1) preparing a reaction solution; 2) causing the reaction solution react for 3 days in a polytetrafluoroethylene reaction kettle, and then cooling to room temperature. An encapsulated [VW12]4-cluster metal organic nanotube micropore crystalline state material modified carbon paste electrode is taken as an electrocatalyst for reducing potassium iodate and is used for efficiently detecting iodate ions. According to the method provided by the invention, the encapsulated [VW12]4-cluster metal organic nanotube micropore crystalline state material can be acquired.

The invention discloses a preparation method for a Schiff base complex modified carbon paste electrode, wherein, 0.03 to 0.050 gram 5-bromosalicylaldehyde condensed taurine Schiff base nickel complexes are dissolved into 1 milliliter N,N-dimethylformamide (DMF) solvent, and then 0.300 to 0.400 gram graphite powder is added into the solvent; the solvent is stirred uniformly and then placed into a vacuum drying chamber for vacuum drying under 50 DEG C, and then electrode materials can be obtained; the electrode materials after drying are pulverized, heated to 80 DEG C together with 0.14 to 0.18 paraffins, uniformly stirred, and then quickly filled into a glass tube for compaction; a copper wire is inserted into the other end while the temperature is high and polished on a weighing paper after cooling, and then the 5-bromosalicylaldehyde condensed taurine Schiff base nickel complex modified carbon paste electrode is prepared. The preparation method is simple; moreover, the prepared electrode has high electrocatalysis oxidation activity on formaldehyde, thereby the preparation method can be applied to analytical measurement of formaldehyde in an environmental sample.

The invention provides a novel carbon paste electrode, which can greatly improve the performances of the carbon paste electrode such as electrical conductivity, electrochemical activity, stability and the like. A carbon paste of the novel carbon paste electrode is made of carbon material powder which is agglutinated by triphenylamine. The carbon paste can greatly improve the electrical conductivity and the electron transfer rate of the electrode, and simultaneously can improve the stability of the electrode. The invention also provides a method for preparing the novel electrode, which comprises the following steps of: mixing a conducting carbon material with the triphenylamine, and then sufficiently grinding and mixing the mixture; stuffing the even mixture into a clean electrode sleeve, compacting the mixture, and inserting a conducting wire in the rear part of the mixture to prepared a semi-finished product of the electrode; and heating the prepared semi-finished product in a baking oven, and when the conducting carbon material is combined with the triphenylamine, cooling the mixture at the normal temperature. The electrode prepared by the method enhances the responses to potassium ferricyanide and dopamine. The mechanical properties and the long-term stability of the electrode are also greatly improved. The electrode has the advantages of simple manufacturing, simple and convenient operation, good repeatability and low cost.

The invention relates to application of a water-soluble quantum dot-mesoporous nickel graphite working electrode in detection of trace amino acid in food. The invention provides the water-soluble quantum dot-mesoporous nickel graphite working electrode with low reagent consumption, high detection speed and high sensitivity and for detecting trace amino acid in food and application of the electrode in detection of the trace amino acid in the food. The water-soluble quantum dot-mesoporous nickel graphite working electrode is prepared by adopting the following steps of: mixing semiconductor, sodium salt or potassium salt, cadmium salt or oxide of cadmium, water-soluble stabilizing agent and water uniformly to prepare water-soluble quantum dots; modifying proper chemical groups on the surfaceof mesoporous nickel; combining the mesoporous nickel and the water-soluble quantum dots; and mixing the combination of the quantum dots and the mesoporous nickel, graphite and paraffin uniformly, then putting the mixture into a glass tube, and putting the prepared water-soluble quantum dot-mesoporous nickel graphite working electrode into a refrigerator for storage. The prepared water-soluble quantum dot-mesoporous nickel graphite working electrode for detecting the trace amino acid in the food is used as a working electrode of a flow injection electrochemical luminous instrument, a proper counter electrode and a proper reference electrode are selected, buffer solution is added into a detector, and then the amino acid in a food sample is detected. The detector has higher selectivity and sensitivity and low reagent consumption, and can be reused.

The invention discloses a paper base micro-fluidic chip for selecting soil active bacterium and components and application of the paper base micro-fluidic chip. A plurality of micro-fluidic units, and units, such as carbon paste three-electrode arrays for bacterial respiration electrochemical measurement are printed on paper to form two types of array dual paper base micro-chamber co-culture and activity screening function units, on chromatographic paper, a carbon paste electrode, a PDMS (Polydimethylsiloxane) passage, a PDMS micro-culture room and the like are sequentially overprinted through silk-screen printing, and furthermore, a model bacterial array unit is printed in an aseptic condition; a dual micro-chamber co-culture array unit of a sample bacterium-model bacterium and a secondary metabolite of a sample bacterium are used to inhibit adjacent model bacterium respiratory chains, and a carbon paste three-electrode printed together with a dual micro-chamber is used to find the position of some active sample bacterial colony; the secondary metabolite of the active sample bacterium is separated through paper base two-dimensional electrophoresis, and an array carbon paste three-electrode is used to find the positions of active components obtained through two-dimensional electrophoresis. The paper base micro-fluidic chip for selecting soil active bacterium is suitable for selecting the active bacterium and active complex components in multiple fields.

The invention provides a method for preparing an ethylene diamine tetraacetic acid (EDTA) modified carbon paste carbon nano tube membrane electrode, relates to a method for preparing a carbon nano tube membrane electrode, and aims to solve the technical problems that in the conventional method for preparing the carbon nano tube membrane electrode, the modifying layer of the electrode tube is not fixed, and the advancing amount of the copper wires is difficult to control. The method comprises the following steps of: 1, preparation of an electrode tube; 2, preparation of an EDTA modified carbon paste electrode: grinding graphite powder and EDTA in a mortar, adding a hydrophobic adhesive, uniformly stirring the mixture to obtain the carbon paste, and filling the carbon paste in an electrode tube shell by extrusion; and 3, preparation of an EDTA modified carbon paste carbon nano tube membrane electrode. According to the method, the EDTA modified carbon paste carbon nano tube membrane electrode has high anti-interference capability and high electrode stability, and is quite suitable for analyzing and monitoring copper ions in water-steam systems of thermal equipment of power plants, refinery plants and chemical enterprises, and moreover, the advancing amount of the copper wires can be accurately controlled.

The invention discloses a method for constructing a modified carbon paste electrode by a hydrotalcite composite material as well as an electrochemical determination method of super-trace heavy metal ions and application of the methods. The methods can accurately, rapidly and sensitively determine the super-trace heavy metal ions in a water sample and belong to the technical field of electrochemical analysis and detection. The heavy metal ions are captured and enriched by a complexation effect of ethylene diamine tetraacetic acid to the heavy ions, such as lead ions, copper ions, zinc ions and cadmium ions; the super-trace heavy metal ions in the water sample are detected by a differentiated pulse stripping voltammetry method; and the detection limit of the detected metal ions can be reduced to 2 nanograms / liter. The method disclosed by the invention has the advantages of simple electrode preparation method, high determination sensitivity, low detection limit and strong anti-interference capability, and can realize accurate determination of the heavy metal ions in the water sample.

The present invention discloses a preparation method of an electrogenerated chemiluminescence sensor with a quantum dot modified nano porous carbon paste electrode for detecting trace antibiotic residues. The preparation method for the carbon paste electrode comprises the following steps: synthesizing nanoporous gold and quantum dot solution according to the existing method; modifying light-emitting quantum dots into the surface and the porous channels of the nanoporous gold material layer by layer by adopting the layer-by-layer self-assembly modification technology; doping the modified nanoporous gold material into graphite powder. The method for detecting the trace antibiotic residues comprises the following steps: connecting the modified carbon paste electrode with an electrogenerated chemiluminescence instrument, and detecting the trace antibiotic residues in dairy and meat products. The sensor has high sensitivity and a high detection speed, and one basic detection process can be completed within only 2-5 minutes. Moreover, the sensor has a low cost, and is economical and practical. The instrument is easy to operate and can automatically record and analyze experimental results, the effect of subjective factors is avoided, and good repeatability is achieved.

The invention discloses a preparation method of a nanometer VN / graphene composite paste electrode sensor. The preparation method comprises following steps: firstly, sulfuric acid is adopted for pre-treatment of oxidized graphene so as to obtain pre-treated oxidized graphene; by mass, 42 to 45% of nanometer VN, 20 to 25% of the pre-treated oxidized graphene, 12 to 18% of N-ethyl-3-methylpyridine hexafluorophosphate, 8 to 12% of liquid paraffin, and 6 to 10% of petroleum ether are introduced into a agate mortar for uniform grinding, an obtained carbon paste is introduced into a glass tube connected with a wire, compressing, drying, polishing with abrasive paper for metallograph, polishing, and deionized water washing are carried out so as to obtain a nanometer VN / graphene composite paste electrode, wherein the internal diameter of the glass tube is 5mm. The electric conductivity of the nanometer VN / graphene composite paste electrode is increased by 2 to 4 times of that of common paste electrode; the electrochemical window is wide; the preparation method is simple; cost is low; surface replacing is convenient; residual current is low; the sensitivity and the selectivity in detection of luteolin are high.

Techniques for combining electrochemical measurements of the brain or spinal cord by voltammetry together with a scan such as magnetic resonance imaging or spectroscopy, e.g. fMRI. The techniques use particular microelectrodes, such as carbon fibre or carbon paste electrodes which do not affect the magnetic resonance measurements. The techniques allow the correlation of voltammetry and magnetic resonance measurements which in turn allows one to be used for substitution of the other in appropriate circumstances and also allows the translation of results in animal models to the human model.

The invention provides a carbon paste electrode, which comprises an electrode pipe shell, a carbon paste filled inside the electrode pipe shell and an electrode lead wire led out of the inside of the electrode pipe shell. The electrode lead wire is connected with the carbon paste. The outside of the electrode pipe shell is sleeved with an updating brush which can rotate relative to the electrode pipe shell. The updating brush is connected with a rotation driving device which drives the updating brush to rotate relatively around the electrode pipe shell. The invention also provides a preparation method for the carbon paste electrode, which comprises the following steps: filling the carbon paste inside the electrode pipe shell; arranging one end of the electrode lead wire inside the electrode pipe shell and connecting the end of the electrode lead wire with the carbon paste, and leading the other end of the electrode lead wire out of the inside of the electrode pipe shell; sleeving the updating brush outside the electrode pipe shell, wherein the update brush can rotate relative to the electrode pipe shell; and connecting the rotation driving device on the updating brush, wherein therotation driving device can drive the updating brush to rotate relatively around the electrode pipe shell.

The invention discloses a method for measuring the antigen of ovarian cancer embryo by an electrochemical luminescence (ECL) immunosensor. The method comprises the following steps of: producing bioactivator-carried Fe3O4 magnetic nano particles by an immune sandwich method; and modifying the particles on the surface of a solid paraffin carbon paste electrode (SCPE), developing an easily updated immunosensor, and measuring the CA125 antigen by an ECL method. Glucose is catalyzed and oxidized by glucose oxidase (GOD) to generate H2O2, and an ECL signal is generated by a Luminol. The ECL signal is observably enhanced along with the increase of the GOD-labeled second antibody concentration. The sensor is good in response to the CA125, and the linear range of the sensor is 0.01-10mU / mL. The method is high in sensitivity, good in selectivity, high in specificity, easy to update, and wide in application scope.

The invention discloses a preparation method and application of an NH2-MIL-125 modified carbon paste electrode, relating to the technical field of electrochemical detection. The method comprises the following steps: grinding graphite powder, NH2-MIL-125 (Ti) solid powder and liquid paraffin into uniform carbon paste, and filling the carbon paste in an adjustable carbon paste electrode cavity body to obtain the NH2-MIL-125 modified carbon paste electrode. Under the irradiation of visible light, the cathodic stripping voltammetry detection of an oxidative product after photocatalytic oxidation of NH2-MIL-125 (Ti) on Mn<2+> is performed, and relatively good sensitivity and selectivity can be realized as a standard curve method is adopted. The NH2-MIL-125 modified carbon paste electrode is applied to detection of the Mn<2+> content, the detection result of the method is nearly the same as that of atomic absorption spectrometry, and a novel method for detecting the manganese ion content is formed.

The invention discloses an amperometric enzyme electrode based on an electrostatic spinning fibrous membrane and a method for preparing the same. The method uses dimethyl formamide as solvent, a platinum electrode, a gold electrode, a glassy carbon electrode or a carbon paste electrode as a receiving electrode and acrylonitrile copolymer as raw materials, and deposits a copolymer fibrous membrane layer on the surface of the electrode through the electrostatic spinning method. The method is as follows: firstly, a monolayer of redoxase is constructed on the surface of fibers through the covalent method; secondly, bifunctional reagent is added into enzyme solution, and enzyme congeries is driven to be covalently fixed on the enzyme membrane on the surface of the electrode. In the amperometric enzyme electrode, due to large specific surface area and high porosity of the fibrous membrane and the unique enzyme immobilization method, the electrochemical enzyme electrode is characterized by large response strength, high stability, wide linear detection range and so on. The method for preparing the enzyme electrode is simple and is suitable for mass production, and the enzyme electrode can be reused and preserved for a long term.

The invention provides a carbon paste electrode. The carbon paste electrode comprises an electrode tube shell, a carbon paste filled in the electrode tube shell, and an electrode lead contacted with the carbon paste and led out from the electrode tube shell, wherein the carbon paste comprises graphite and a DNOP (di-n-octyl phthalate). The carbon paste electrode provided by the invention is low in residue current, simple in preparation method, non-toxic, easy to update the surface, low in price and wide in potential use range; the hydrogen overpotential is 200 mV higher than the carbon paste mixed by silicone oil and graphite powder and the reproducibility is good. The invention further provides a preparation method of the carbon paste electrode.

The invention discloses a chemically modified carbon paste electrode which comprises an electrode tube, carbon paste filled in the electrode tube as well as an electrode lead contacted with the carbon paste and lead out from the electrode tube, wherein the carbon paste comprises a conductive carbon material, a bonding agent and a composite modifier; the composite modifier comprises bismuth-contained solid powder and a chelating agent; the bismuth-contained solid powder adopts solid bismuth substances which are insoluble with water; and the chelating agent is a hydrophobic organic reagent and can form a chelate with at least one of lead ions, cadmium ions and zinc ions. The detection limit of the chemically modified carbon paste electrode to lead ions, cadmium ions and zinc ions reaches 1.0*10<-12> M; and compared with the detection limit of a conventional chemically modified carbon paste electrode, the detection limit is reduced by 1-3 orders of magnitude, and the detection performance is remarkably improved on the basis of the conventional chemically modified carbon paste electrode.

The invention relates to the technical field of medical detection and particularly relates to a sweat lactic acid rapid detection device. The device comprises a flexible substrate layer, an electrode layer, an isolation layer and an electronically monitoring layer. The flexible substrate layer is made of a porous flexible material. The electrode layer comprises a counter electrode, a work electrode, a reference electrode and a reaction chamber. The work electrode comprises a second carbon paste lead, a carbon paste electrode III and a reaction electrode. The reaction electrode comprises an oxidase layer and a three-dimensional graphene composite material layer. The three-dimensional graphene composite material layer is prepared from nanometer TiO2 uniformly dispersed on graphene. The three-dimensional graphene composite material layer has a hollow microsphere network structure having pore size distribution of 20nm-40nm and a specific surface area of 1500 m<2> / g-1650 m<2> / g. The isolation layer is bonded to an insulation layer. The electronically monitoring layer comprises a voltage control unit, a current measurement unit and a data finishing unit. The detection device is free of blood sampling, can continuously monitor and detect the amount of human lactic acid and has the characteristics of high detection sensitivity, strong stability and carrying convenience.

The invention discloses a preparation method of a superoxide dismutase modified vanadium nitride paste electrode sensor, characterized by comprising the following steps: firstly preparing a nano VN / graphene compound paste electrode by adopting nano VN, graphene oxide and N-ethyl-3-picoline hexafluorophosphate; then modifying the nano VN / graphene compound paste electrode by adopting gamma-aminopropyl triethoxysilane, so as to obtain an aminopropyl triethoxysilane modified electrode; finally, dissolving bovine serum albumin and superoxide dismutase by adopting a phosphate buffer solution to prepare a superoxide dismutase immobilizing liquid; and then dripping the superoxide dismutase stationary liquid onto the aminopropyl triethoxysilane modified electrode, so as to prepare the superoxidedismutase modified vanadium nitride paste electrode sensor. The electrode has the advantages of being improved in conductivity in comparison with common carbon paste electrodes, wide in electrochemical window, simple in preparation method, low in cost, easy to update in surface, and small in residual current. A detection superoxide radical is high in sensitivity and good in selectivity.

The invention discloses application of CoTPyP porphyran coordination polymer materials. According to the application, CoTPyP porphyran coordination polymers are prepared into a carbon paste electrode; the carbon paste electrode is used as an electrochemical sensor to be applied to nitrite electrocatalysis, wherein the chemical formula of the CoTPyP porphyran coordination polymers is [Co(TPyP)]6 12CH3COOH 12 H2O. The electrocatalysis effect of the electrochemical sensor on nitrite is studied; the result shows that the sensor has better performance; the minimum detection limit is 2.0*10<8>M; the sensitivity is 743.3mA mol<-1> L cm<-2>; the linear range is 2.5*10<-6> to 5.5*10<-4>M, wherein R is 0.993; the surface has the characteristics of high sensitivity, short response time and the like; certain application values are realized in the aspect of electrochemical sensing.

The invention relates to a method for preparing an electrochemical sensor based on both a synthetic zinc oxide nano-rod and a novel 5-(4'-amino-3'-hydroxyl-diphenyl-4-alcohol)-acrylic acid modified carbon paste electrode. The electrochemical sensor is used for detecting levodopa with existence of carbidopa, is simple to operate, high in response speed, good in repeatability and low in price and has a lower detection limit; during detection, the time-consuming extraction step is not needed, influence from pH is avoided, reaction with oxygen does not happen and innocuity is ensured; therefore, the electrochemical sensor is simple, accurate, precise, low-price and quick in detection, and has a relatively good application prospect in clinical practice that carbidopa and levodopa are detected at the same time.