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Method for preparing water-soluble quantum dot carbon paste electrode for detecting trace amino acid in food

A quantum dot and amino acid technology, applied in the field of amino acid detection in food, can solve the problems of complex detection process, large amount of reagents, low detection sensitivity, etc., and achieve the effect of improving sensitivity

Inactive Publication Date: 2011-05-04
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But its minimum detection limit is 10pmol, and this method is not suitable for the determination of fruits, vegetables, beverages and starchy foods with low protein content
At the same time, due to the strict acidity conditions to be controlled in the determination of various amino acids in the test, the technical cost is relatively high, and the required time is generally long, some even as long as several hours. The operation is complicated and cannot be used. Rapid detection on site
[0008] 4. For the detection and analysis of trace amino acids in food, the above methods generally have disadvantages such as low detection sensitivity, high cost, complicated detection process, single detection, large amount of reagents, and not suitable for on-site rapid detection, so they cannot meet the actual conditions. testing needs

Method used

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  • Method for preparing water-soluble quantum dot carbon paste electrode for detecting trace amino acid in food
  • Method for preparing water-soluble quantum dot carbon paste electrode for detecting trace amino acid in food
  • Method for preparing water-soluble quantum dot carbon paste electrode for detecting trace amino acid in food

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 (water-soluble quantum dots: CdTe; essential amino acids, such as lysine)

[0034] A CdTe quantum dot-mesoporous nickel graphite carbon paste working electrode for detecting trace amounts of lysine in food, the preparation method comprising the following steps:

[0035] (1) Te powder and NaBH 4 The molar ratio is 1:2, in N 2 Under protection, react in a water bath at 80°C for 2h to purple. The solution was placed in an ice-water bath to allow Na 2 B 2 o 7 Crystallization, the upper layer is NaHTe solution;

[0036] (2) Weigh 0.1236g CdCl 2 2.5H 2 O to a 100mL round bottom flask, add 290mL of secondary water to dissolve, add 45μL TGA, and use 1mol·L -1 NaOH solution to adjust the pH to 9, fill with N 2 Stir for 20min, after heating to reflux;

[0037] (3) Quickly add NaHTe solution to the solution prepared in (2), heat and reflux at 80° C., obtain quantum dots with different particle sizes according to different times, and store the quantum dot sol...

Embodiment 2

[0045] Embodiment 2 (water-soluble quantum dots: CdTe; non-essential amino acids, such as glutamic acid)

[0046] A CdTe quantum dot-mesoporous nickel graphite carbon paste working electrode for detecting trace amounts of glutamic acid in food, the preparation method comprising the following steps:

[0047] (1) Te powder and NaBH 4 The molar ratio is 1:2, in N 2 Under protection, react in a water bath at 80°C for 2h to purple. The solution was placed in an ice-water bath to allow Na 2 B 2 o 7 Crystallization, the upper layer is NaHTe solution;

[0048] (2) Weigh 0.1236g CdCl 2 2.5H 2 O to a 100mL round bottom flask, add 290mL secondary water to dissolve, add 45μL TGA, and use 1mol·L -1 NaOH solution to adjust the pH to 9, fill with N 2 Stir for 20min, after heating to reflux;

[0049] (3) Quickly add NaHTe solution to the solution prepared in (2), heat and reflux at 80° C., obtain quantum dots with different particle sizes according to different times, and store the ...

Embodiment 3

[0057] Embodiment 3 (water-soluble quantum dots: CdSe; essential amino acids, such as tryptophan)

[0058] A CdSe quantum dot-mesoporous nickel graphite carbon paste working electrode for detecting trace amounts of tryptophan in food, the preparation method comprising the following steps:

[0059] (1) Mix Se powder with NaBH 4 The molar ratio is 1:3, in N 2 Under protection, react in a water bath at 80°C for 1.5h. The solution was placed in an ice-water bath to allow Na 2 B 2 o 7 Crystallization, the upper layer is nearly colorless or slightly yellowish clear liquid is NaHSe solution;

[0060] (2) Weigh 0.1236g CdCl 2 2.5H 2 O to a 100mL round bottom flask, add 290mL of secondary water to dissolve, add 80μL TGA, and use 1mol·L -1 NaOH solution to adjust the pH to 11, fill with N 2 Stir for 20min, after heating to reflux;

[0061] (3) Quickly add NaHSe solution to the solution prepared in (2), heat and reflux at 80° C., obtain quantum dots with different particle size...

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Abstract

The invention relates to application of a water-soluble quantum dot-mesoporous nickel graphite working electrode in detection of trace amino acid in food. The invention provides the water-soluble quantum dot-mesoporous nickel graphite working electrode with low reagent consumption, high detection speed and high sensitivity and for detecting trace amino acid in food and application of the electrode in detection of the trace amino acid in the food. The water-soluble quantum dot-mesoporous nickel graphite working electrode is prepared by adopting the following steps of: mixing semiconductor, sodium salt or potassium salt, cadmium salt or oxide of cadmium, water-soluble stabilizing agent and water uniformly to prepare water-soluble quantum dots; modifying proper chemical groups on the surfaceof mesoporous nickel; combining the mesoporous nickel and the water-soluble quantum dots; and mixing the combination of the quantum dots and the mesoporous nickel, graphite and paraffin uniformly, then putting the mixture into a glass tube, and putting the prepared water-soluble quantum dot-mesoporous nickel graphite working electrode into a refrigerator for storage. The prepared water-soluble quantum dot-mesoporous nickel graphite working electrode for detecting the trace amino acid in the food is used as a working electrode of a flow injection electrochemical luminous instrument, a proper counter electrode and a proper reference electrode are selected, buffer solution is added into a detector, and then the amino acid in a food sample is detected. The detector has higher selectivity and sensitivity and low reagent consumption, and can be reused.

Description

technical field [0001] The invention relates to the technical field of amino acid detection in food, more specifically a water-soluble quantum dot detector for detecting trace amino acids in food, and the invention also relates to the use of the water-soluble quantum dot carbon paste electrode in the detection of food Application of trace amino acids. Background technique [0002] Amino acid is a general term for a class of organic compounds containing amino and carboxyl groups. Amino acid is the most basic substance that constitutes biological proteins and is related to life activities. It is the basic unit that constitutes protein molecules in living organisms and has a close relationship with biological life activities. It has special physiological functions in antibodies and is a biological One of the indispensable nutrients in the body. Amino acids play an extremely important role in the human body. For example, amino acids are the basic substances that constitute the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N21/76
Inventor 于京华王少伟颜梅杨萍万夫伟卢娟娟于克孝
Owner UNIV OF JINAN
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