Preparation and catalytic application of Nafion/horseradish peroxidase/tricobalt tetraoxide-graphene/ionic liquid carbon paste electrode

A technology of horseradish peroxidase and cobalt tetroxide is applied in the field of catalysis applications and biosensors, which can solve the problems that direct electrochemistry is difficult to achieve, and achieve the effects of excellent conductivity, high sensitivity and convenient use.

Active Publication Date: 2016-07-13
HAINAN NORMAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Since the redox centers of most enzymes are deeply buried in their internal three-dimensional matrix structures, direct electrochemistry is often difficult to achieve

Method used

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  • Preparation and catalytic application of Nafion/horseradish peroxidase/tricobalt tetraoxide-graphene/ionic liquid carbon paste electrode
  • Preparation and catalytic application of Nafion/horseradish peroxidase/tricobalt tetraoxide-graphene/ionic liquid carbon paste electrode
  • Preparation and catalytic application of Nafion/horseradish peroxidase/tricobalt tetraoxide-graphene/ionic liquid carbon paste electrode

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Embodiment 1

[0024] 1-Hexylpyridine hexafluorophosphate (HPPF 6 ) Modified carbon paste electrode preparation: weigh 1.6g graphite powder and 0.8g ionic liquid (HPPF 6 ) into a mortar and grind evenly, fill the ground mixture into a glass electrode tube with an inner diameter of 4mm, insert a copper wire as a wire, and compact the solid mixture in the glass electrode tube to obtain ionic liquid-modified carbon Paste electrode (CILE), the electrode surface is polished to a mirror surface before use.

Embodiment 2

[0026] Nafion / HRP / Co 3 o 4 - Preparation of GR / CILE: prepare 15mg / mLHRP and 0.5mg / mLCo respectively 3 o 4 -GR solution, take 0.5mg / mLCo 3 o 4- 6 μL of GR solution was dripped on the surface of the electrode, and after natural drying, 6 μL of 15 mg / mL HRP solution was applied on the surface of the electrode. After natural drying, 8 μL of 0.5% Nafion was applied on the surface of the electrode to form a film to fix HRP, and the modified electrode Nafion / HRP / Co 3 o 4 -GR / CILE.

Embodiment 3

[0028] figure 2 It is the ultraviolet absorption spectrum curve of HRP and HRP in HRP / Co3O4-GR composite material in PBS buffer solution of pH 3.0, and the experimental instrument used is TU-1901 double-beam ultraviolet-visible spectrophotometer. The Soret absorption band of HRP molecule in PBS is located at 403.0nm (curve a), HRP and Co 3 o 4 - The Soret absorption band of the GR mixed solution in the TU-1901 double-beam UV-visible spectrophotometer is also at the same position (curve b), indicating that the HRP molecule is in the HRP / Co 3 o 4 -GR composites maintain their original structure without denaturation.

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Abstract

The invention relates to a biosensor, particularly relates to a preparation method and catalytic application of a Nafion/horseradish peroxidase/tricobalt tetraoxide-graphene/ionic liquid carbon paste electrode (Nafion/HRP/Co3O4-GR/CILE) and belongs to the technical field of electrochemical analysis and detection. According to the preparation method of the Nafion/HRP/Co3O4-GR/CILE, an ionic liquid carbon paste electrode is used as a substrate electrode, and by virtue of the specific advantages of a Co3O4-GR nano composite material of large surface area and good biocompatibility, HRP is adsorbed on the surface of the material; and furthermore, the material is fixed by a Nafion film to prepare the Nafion/HRP/Co3O4-GR/CILE. HRP in the modified electrode keeps a natural structure and biological activity. Research on electrochemical properties of the modified electrode shows that the modified electrode has good electrochemical catalysis capability on trichloroacetic acid (TCA), and can be used for effectively detecting TCA, and the detection limit is 0.33mmol/L. The preparation method and the catalytic application, provided by the invention, do not cause environmental pollution; the modified electrode has good stability, repeatability and sensitivity, and the preparation process is simple, low in cost and easy to operate, so that the modified electrode has wide social benefits.

Description

technical field [0001] The invention relates to a biosensor, specifically a Nafion / horseradish peroxidase / cobalt tetroxide-graphene / ionic liquid carbon paste electrode (Nafion / HRP / Co 3 o 4 -GR / CILE) preparation method and catalytic application; it belongs to the technical field of electrochemical analysis and detection. Background technique [0002] Horseradish peroxidase (Horseradish Peroxidase, HRP) is a heme-containing protein with high specific activity, stability, small molecular weight, and easy preparation of pure enzyme. It is one of the most widely studied analytical enzymes. Oxidoreductase-based direct electron transfer electrochemical biosensors have attracted great attention due to their excellent selectivity, sensitivity, and convenience, and have been widely used in pharmaceutical monitoring, clinical diagnosis, and food analysis. [0003] The construction of enzyme biosensors needs to solve two key problems, one is how to immobilize the enzyme on the electro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327G01N27/30
CPCG01N27/30G01N27/3278
Inventor 孙伟牛学良郑雯文作瑞王文成闫丽君
Owner HAINAN NORMAL UNIV
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