Method for measuring antigen of ovarian cancer embryo by electrochemical luminescence (ECL) immunosensor

A technology for luminescence immunity and ovarian cancer, applied in chemiluminescence/bioluminescence, material electrochemical variables, scientific instruments, etc., to achieve the effect of low detection limit, wide linear range and high sensitivity

Inactive Publication Date: 2012-05-02
GUILIN UNIVERSITY OF TECHNOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

CA125 electrochemiluminescence immunosensor combined with magnetic nanoparticles has not been reported

Method used

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  • Method for measuring antigen of ovarian cancer embryo by electrochemical luminescence (ECL) immunosensor
  • Method for measuring antigen of ovarian cancer embryo by electrochemical luminescence (ECL) immunosensor
  • Method for measuring antigen of ovarian cancer embryo by electrochemical luminescence (ECL) immunosensor

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Experimental program
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Embodiment

[0019] Specific steps are as follows:

[0020] (1) Cut out an iron rod (outer diameter 2.55mm) about 5cm in length and a glass tube with a length of 3cm (3.0mm, id), grind and clean both ends of the glass tube, and mix solid paraffin and wax in a ratio of 3:1. Toner, heat until the paraffin melts, stir evenly, insert an iron rod at one end of the glass tube, fill the above mixed paraffin carbon paste from the other end of the glass tube, and use it on smooth printing paper and suede in turn after cooling 1.0~0.05μm Al 2 o 3 The surface of the electrode was polished with powder; before use, the surface of the electrode was cleaned with nitric acid, absolute ethanol, and double-distilled water with a volume percentage of 1:1, and washed in 1mol / L H 2 SO 4 The solution is activated by cyclic voltammetry (CV) within the scanning range of -1.2 to 1.2V;

[0021] (2) Synthesis of Fe by chemical co-precipitation method 3 o 4 Magnetic nanoparticles: according to Fe 2+ with Fe 3...

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Abstract

The invention discloses a method for measuring the antigen of ovarian cancer embryo by an electrochemical luminescence (ECL) immunosensor. The method comprises the following steps of: producing bioactivator-carried Fe3O4 magnetic nano particles by an immune sandwich method; and modifying the particles on the surface of a solid paraffin carbon paste electrode (SCPE), developing an easily updated immunosensor, and measuring the CA125 antigen by an ECL method. Glucose is catalyzed and oxidized by glucose oxidase (GOD) to generate H2O2, and an ECL signal is generated by a Luminol. The ECL signal is observably enhanced along with the increase of the GOD-labeled second antibody concentration. The sensor is good in response to the CA125, and the linear range of the sensor is 0.01-10mU / mL. The method is high in sensitivity, good in selectivity, high in specificity, easy to update, and wide in application scope.

Description

technical field [0001] The invention relates to a method for measuring ovarian carcinoembryonic antigen with an electrochemiluminescence immunosensor. Background technique [0002] The magnetic nanoparticles have good biocompatibility and can be used for immobilization of biomolecules; the specific surface area is large, which can improve the sensitivity of detection. Magnetic nanoparticles-immunosensors combine highly sensitive sensing technology with specific immune responses. Magnetic nanoparticle-sensitive film electrochemical sensors are usually detected by voltammetry or potentiometric method. Electrochemiluminescence (ECL) is a specific chemiluminescence reaction induced by electrochemistry on the electrode surface, including two processes of electrochemistry and chemiluminescence. ECL detection has high sensitivity, strong specificity, good repeatability and fast detection speed. Combining magnetic nanoparticles with immunoassays is used to make magnetic nanoparti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574G01N27/26G01N21/76
Inventor 李建平徐倩
Owner GUILIN UNIVERSITY OF TECHNOLOGY
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