94 results about "Dna sensor" patented technology

The present invention relates to methods and compositions for identifying a pathogen. The inventions provide an antibody-based biosensor probe comprising (AUNT) in combination with a polymer-coated magnetic nanoparticle. In particular, a nanoparticle-based biosensor was developed for detection of Escherichia coli O157:H7 bacterium in food products. Further described are biosensors for detecting pathogens at low concentrations in samples. Even further, a gold nanoparticle-based electrochemical biosensor detection and amplification method for identifying the insertion element gene of Salmonella enterica Serovar Enteritidis is described. The present invention provides compositions and methods for providing a handheld potentiostat system for detecting pathogens outside of the laboratory. The AUNT biosensor system has applications detecting pathogens in food, water, beverages, clinical samples, and environmental samples.

The invention discloses preparation of an ECL (electro chemical luminescence) DNA (Deoxyribose Nucleic Acid) sensor based on a 3D paper chip, and application of the sensor to simultaneous detection on Hg<2+> and Ag<+>. The preparation method of the sensor (the schematic diagram of which is shown in the figure) comprises the following steps of: designing a micro-fluidic chip pattern on a computer, and preparing a 3D paper chip sensor; preparing nano materials such as a porous gold nanowire, PdAg alloy, a carbon point and P acid according to existing methods, preparing PdAg@CQDs and PdAg@P-acid with signal amplification functions as ECL probes, and respectively compounding the ECL probes with DNA subjected to specific binding with Hg<2+> and Ag<+>; and modifying the porous gold nanowire to the surface of an electrode of the sensor by an electrode surface modifying technology, and absorbing a DNA segment to prepare the DNA sensor. The method for simultaneously detecting Hg<2+> and Ag<+> comprises the following steps of: connecting the modified electrode to an ECL apparatus, and detecting Hg<2+> and Ag<+> in a sample extracting solution. The electrode of the sensor is strong in specificity and high in sensitivity, and is capable of reaching a p mol level. When the paper chip sensor easily processed is used for simultaneously detecting two materials on the same electrode, the detection efficiency is improved, and the cost is lowered.

The invention discloses a novel electrochemical DNA sensor constructed by a nanogold and partially-reduced graphene oxide (p-RGO) modified ionic liquid carbon paste electrode (CILE) as a platform prepared by an electrochemical method, and a method of applying the CILE to detect the Listeria feature gene sequence. 1-hexylpyridiniumhexafluorophosphate as a modifier is used for preparing the substrate electrode CILE, gold nanoparticles are deposited on the surface of the substrate electrode CILE and then the p-RGO is prepared by controlling electrochemical reduction conditions; an amino-modified probe ssDNA is fixed on the surface of the electrode by an amido bond covalent bonding method through carboxyl groups remained on the surface of p-RGO to constitute ssDNA/p-RGO/AuNPs/CILE; methylene blue (MB) as an indicator is used for detecting the hybridization reaction after a target sequence is hybridized, and a differential pulse voltammetry (DPV) is used for detecting the Listeria feature gene segment.

The invention relates to an electrochemical DNA sensor based on a graphene-precious metal composite and a preparation method thereof. The preparation method comprises the following steps: (1) preparing an aqueous dispersion of graphene oxide from graphite powder by utilizing a Hummer method; (2) preparing a nano-precious metal particle/graphene composite by a chemical reduction method; and (3) adhering the precious metal/graphene composite onto a glassy carbon electrode, modifying with single-stranded DNA, and hybridizing the modified electrode and a target gene so as to obtain the electrochemical DNA sensor. According to the electrochemical DNA sensor based on a graphene-precious metal composite, a detection limit of DNA can be greatly increased to 1.2*10<-10>mol/L.

A card shooter apparatus (1) in a table game system a card shooter unit (4) including a card storage unit (2) that stores a plurality of cards (100) in a horizontal stack. A control device (5) constituted by a program storage unit, a computer device, or the like is placed in a lower part of the card shooter apparatus (1). A transparent bottom plate (9) is provided on a bottom of the card storage unit (2). An optical sensor (10) that receives reflected lights of lights applied to end surfaces of the plurality of cards (100) stored in the card storage unit (2), and a DNA sensor (11) that obtains DNA information from a DNA-containing coating applied to the cards (100) stored in the card storage unit 2 are placed below the bottom plate (9).

The invention relates to a preparation method and a use of an electrochemical DNA biosensor based on Hg and Pb ion-induced DNA configuration change and belongs to the technical field of analytical chemistry and chemical sensors. The preparation method provided by the invention comprises the following steps of 1, designing a functional zone composed of two parts of DNA sequences respectively used for specifically recognizing Hg ions and Pb ions and 2, assembling the functional zone on the surface of an Au electrode to obtain an electrochemical DNA biosensor. The electrochemical DNA biosensor obtained by the preparation method can be used for detecting a single Hg/Pb system of practical water and can also be used for separately detecting Hg and Pb ions of a mixed system through use of a masking agent. The preparation method of the electrochemical DNA biosensor allows mild conditions, is simple and convenient, and realizes good stability. The electrochemical DNA biosensor has high specificity, high sensitivity and high selectivity, can be operated simply and avoids labeling during detection.

The invention discloses a preparation method for a surface plasmon resonance (SPR) DNA sensor based on graphene oxide, and belongs to the technical field of nanometer material biology. The technical problem to be solved in the preparation method is that the distinctive effect of graphene oxide-DNA is utilized, the surface plasmon resonance technology and the signal amplification mechanism of the AuNPs are applied, based on the competitive inhibition method, the phenomenon that the single-stranded DNA with different concentrations is absorbed on the surface of a sensing chip to cause SPR spectral change, and the single-stranded DNA is detected through the linear change of the resonance angle. Through the adoption of the preparation method, the SPR technology is adopted, the GO (Graphene Oxide) is utilized to assemble the surface of the chip, the single-stranded DNA is sensitively detected through adopting the competitive inhibition method and the AuNPs signal amplification effect, the concentration of the single-stranded DNA is quantitatively detected through analyzing the change of the SPR peak, and the detection limit is ultra-low. The preparation method has the advantages as follows: the instrument and equipment are low in cost, the cost is low, the operation is simple, the efficiency is high, the precision is high, and the detection limit is ultra-low.

Deck information representing a deck of cards (100) is encoded by the DNA contained in the DNA ink (DNA-containing coating) with which the markings (101) representing card suit or rank are printed. The cards (100) are guided along rails (19) in a card shoe (3). A DNA sensor (13) is provided on the sliding surface (17) of the card shoe (3). When light from a light source of the DNA sensor (13) is shined on the DNA-containing coating of the marking (101) printed on the card (100), the light reflected from the DNA-containing coating (light of a prescribed frequency) is received by the DNA sensor (13). When the deck information is read from the DNA, the card (100) is verified using said deck information and the results of the verification are output, thereby allowing on-the-spot detection of cheating in card games as well as preventing those who have obtained cards (100) dishonestly from reproducing said cards (100).

A method for identifying people involves breathing respired air into a collector unit, trapping condensate from the respired air in the collector unit, introduction of the condensate by a same introduction to a DNA sensor unit, analysis of the condensate after a cell disruption, comparison of the result the data from a databank and output of the comparison result with analysis of the identity of a person.

The electrical conductivity of DNA and other oligonucleotide constructs is dependent on its conformational state. Such a dependence may be harnessed for the electronic sensing of external analytes, for instance, adenosine or thrombin. Such a DNA sensor incorporates an analyte receptor, whose altered conformation in the presence of bound analyte switches the conformation, and hence, the conductive path between two oligonucleotide stems, such as double-helical DNA. Two distinct designs for such sensors are described that permit significant electrical conduction through a first or “detector” double-helical stem only in the presence of the bound analyte. In the first design, current flows through the analyte receptor itself whereas, in the second, current flows in a path adjacent to the receptor. The former design may be especially suitable for certain categories of analytes, including heterocycle-containing compounds such as adenosine, whereas the latter design should be generally applicable to the detection of any molecular analyte, large or small, such as the protein thrombin. Since analyte detection in these DNA sensors is electronic, the sensors may be used in rapid and automated chip-based detection of small molecules as well as of proteins and other macromolecules.

The electrical conductivity of DNA and other oligonucleotide constructs is dependent on its conformational state. Such a dependence may be harnessed for the electronic sensing of external analytes, for instance, adenosine. Such a DNA sensor incorporates an analyte receptor, whose altered conformation in the presence of bound analyte switches the conformation, and hence, the conductive path between two oligonucleotide stems, such as double-helical DNA. Two distinct designs for such sensors are described that permit significant electrical conduction through a first or “detector” double-helical stem only in the presence of the bound analyte. In the first design, current flows through the analyte receptor itself whereas, in the second, current flows in a path adjacent to the receptor. The former design may be especially suitable for certain categories of analytes, including heterocycle-containing compounds such as adenosine, whereas the latter design should be generally applicable to the detection of any molecular analyte, large or small. Since analyte detection in these DNA sensors is electronic, the potential exists for their application in rapid and automated chip-based detection of small molecules as well as of proteins and other macromolecules.

The invention provides an aflatoxin B1 aptamer, a DNA sensor, a kit and application. The aflatoxin B1 aptamer has the nucleotide sequence as follows: 5'-GTTmmmmmmmTGTTGTCTCTCT GTGTCTnnnnnnnTTCGCTAGGCCCACA-3', each m and n independently are A or T or C or G, and the m chain segment and the n chain segment achieve complementary pairing. According to the aflatoxin B1 aptamer, the DNA sensor, the kit and the application, no large instrument equipment is needed when the aflatoxin B1 is measured, and therefore the cost is low; a constant-temperature reaction is achieved, qualitative detection of naked eyes can be achieved, and quantitative detection can also be achieved; in addition, no fluorochrome is needed, and therefore the cost is greatly reduced.

The invention relates to the field of detection of mercury ions in environments and particularly relates to preparation of an Ag@Au core-shell structure modified by desoxyribonuclease. The Ag@Au core-shell structure is applied to construction of an electrochemical DNA (Deoxyribonucleic Acid) sensor for detecting the mercury ions. According to a method for preparing a Ag@Au core-shell nano material and a method for detecting the mercury ions by the Ag@Au core-shell nano material, the Ag@Au core-shell structure is prepared and nano particles are modified by the desoxyribonuclease; the Ag@Au core-shell structure is used as a label for constructing the electrochemical DNA sensor for detecting heavy metal ions Hg<2+>. The preparation method of the nano material is simple and rapid and is low in cost; the DNA sensor taking the nano material as the label has relatively good selectivity, stability and repeatability.

Disclosed is a method for detecting 1,8-diaminonaphthalene based on an electrochemical DNA biosensor. The invention belongs to the technical field of electrochemical detection and chemical sensors for detecting 1,8-diaminonaphthalene based on a gold electrode modified by hairpin DNA, and relates to preparation of the gold electrode modified by hairpin DNA which has an electrochemical response to the 1,8-diaminonaphthalene and detection of the 1,8-diaminonaphthalene by adopting the electrode. Specifically, the preparation of the gold electrode comprises using hairpin DNA modified by dithio as a raw material, and self-assembling on the gold electrode surface through the action of gold-sulfur bonds to prepare the electrode modified by the hairpin DNA. Changes in impedance of a DNA film are caused by interaction between the electrode modified by the hairpin DNA and the 1,8-diaminonaphthalene, and detection of 1,8-diaminonaphthalene can be carried out according to responded changes in the impedance. A method for preparing the DNA biosensor of the invention has the advantages of mild conditions, simplicity and convenience, good stability, high sensitivity and simple operation.

The invention discloses a method of testing PML/RAR alpha genes through an electrochemical biosensor of a carbon dot @ graphene oxide composite material. The method comprises the steps that (1) a specific probe is designed according to a gene segment to be tested, wherein the PML/RAR alpha fusion genes of acute promyelocytic leukemia are used as an example; (2) capture probes are self-assembled on the C-dots @ GO/GCE surface through amido bonds, and after hybridization of a DNA electrochemical sensor formed with methylene blue (MB) as the electrochemical hybridization indicator with a solution to be tested, whether the solution to be tested contains the PML/RAR alpha fusion genes or not is judged according to difference of electrical signals. The C-dots @ GO nanocomposite prepared through the method has good electrical conductivity, and abundant carboxyl on the GO surface can increase the number of the capture probes; the DNA sensor formed on such basis has the advantages of good selectivity and high sensitivity.

The invention discloses a vibrio DNA electrochemical sensor and a preparation method and application thereof. The method comprises the steps that graphene oxide is stripped in a N,N-dimethyl formamidesolution containing amino ion liquid, and ion liquid functional graphene is prepared; then, hydrotalcite nano-sheets are prepared in the dispersion solution, an ion liquid functional graphene-like-hydrotalcite nano-sheets composite is prepared, a drip coating method is adopted for preparing a glass carbon electrode modified by the compound, a vibrio probe ssDNA is fixed to the surface of the modified electrode through a biologic connecting agent, and the vibrio DNA sensor is prepared. The DNA sensor has the good sensitivity and selectivity, the low detection limit and the side linear range. The preparation method comprises the steps of preparing the modified electrode material, preparing the electrochemical auxiliary hemolysis vibrio DNA sensor, hybridizing the electrochemical auxiliary hemolysis vibrio DNA sensor with target DNA, and performing sensor electrochemical signal detection.

The present disclosure relates to the use of DNA sample data as part of a biometric security system in a transponder transaction. The biometric security system also includes a DNA sensor that detects biometric samples, and a device for verifying biometric samples. In one embodiment, the biometric security system includes a transponder configured with a DNA sensor. In another embodiment, the system includes a reader configured with a DNA sensor. In yet another embodiment, the present invention discloses methods for proffering and processing DNA samples and DNA sample data to facilitate authorization of transactions.

The present invention discloses a spraying system, particularly a livestock and poultry housing spraying system. The spraying system comprises components as follows: temperature and humidity sensors, a DNA sensor, a harmful gas sensor, an alarm, temperature, humidity, microbes and harmful gas controllers, heating equipment, pulverization system, dormer window switch, fans and gas processors. The spraying system is operated in a full automatic mode. The spraying system can adjust the contents of various factors in livestock and poultry housing according to the feedback information from the sensors, start the pulverization device to perform cooling, disinfecting and immunizing, and start the ventilation device to realize ventilation.

The invention discloses an ionic liquid functional graphene vibrio DNA electrochemical sensor and a preparation method and application thereof. Firstly, ZIF-8 is prepared in graphene oxide dispersion;then a graphene-ZIF-8 compound is subjected to covalent modification by amino functional ionic liquid to prepare an ionic liquid functional graphene-ZIF-8 compound; a gold electrode modified by the compound is prepared by a dispensing method; and then vibrio probe ssDNA is fixed on the surface of the modified electrode by a biological linker so as to prepare the vibrio DNA sensor. The DNA sensorhas excellent sensitivity and selectivity, a low detection limit and a wide linear range. The preparation method comprises: preparation of an electrode modification material; preparation of an electrochemical vibrio parahaemolyticus DNA sensor; hybridization of the electrochemical vibrio parahaemolyticus DNA sensor and target DNA; and sensor electrochemical signal detection. The sensor is simple in operation method and has potential application value.

According to the technical scheme of an electrochemical bladder cancer DNA sensor, carboxylation is carried out on the surface of a glassy carbon electrode by an electrochemical method, and a bladder cancer cell specific DNA probe is assembled on the glassy carbon electrode to prepare the bladder cancer DNA sensor. The glassy carbon electrode is a base electrode, and electrode carboxylation and probe single-stranded DNA assembling are successively carried out. By electrode carboxylation, the probe DNA is fixed. The probe DNA is an recognition element, and a biological bridging agent is 1-ethyl-3-(3-dimethyl aminopropyl)carbodiimide and N-hydroxy succinimide. The preparation method is characterized in that the electrochemical bladder cancer DNA sensor with high sensitivity is obtained. The sensor has high sensitivity, high stability and good selectivity, and testing results of the sensor are better than those of a traditional DNA detection method. The preparation method comprises: 1, preparation of the electrochemical bladder cancer DNA sensor; 2, hybridization of the electrochemical bladder cancer DNA sensor and a target DNA; and 3, electrochemical signal detection of the sensor. The sensor has a simple operation method and is convenient for practical popularization and application.