3 results about "Dna sensor" patented technology

The application provides a DNA sensor based on gold nanoparticles (AuNPs) for visual detection of miRNA-10b. The sensor is obtained by coupling AuNPs-S1 through mercapto between gold nanoparticles (AuNPs) and probe S1; and coupling AuNPs-S2 through mercapto between gold nanoparticles (AuNPs) and probe S2. The combination of the probe and the gold nanoparticles is more firm due to mercapto modification, so that the stability and detection repeatability of the DNA sensor are improved. The aggregation of AuNPs is triggered by hybridization between the target miRNA-10b and the probe, the transition of AuNPs from dispersion to aggregation in the buffer solution is regulated, and the color change from red to blue is visible to the naked eye, so that the rapid and visual detection of the tumor marker miRNA-10b is realized. The linear range is 10 pM-200 nM, and the sensor has excellent stability in serum environments (1%, 10%) and enzymatic environments (5 U / mL DNase and 2 U / mL DNase), and high selectivity. An efficient tool is provided for cancer diagnosis.

This invention discloses a method for studying the mechanism by which the mucoprotein channel 1 (TRPML1) regulates the outflow of zinc ions from lysosomes and induces changes in mitochondrial ATP. This method uses 13 nm AuNPs as loading materials to construct a logic-gated sensor for pH and zinc ion regulation within lysosomes and for TRPML1 mRNA in the cytoplasm. It also uses 5 nm AuNPs as loading materials to construct a sensor targeting mitochondrial ATP for cell imaging. This method enables the detection of mRNA and zinc ions in the cytoplasm, lysosomes, and mitochondria. 2+ ATP-associated ATP sensors, Zn 2+ Three sensors—a sensor, an mRNA sensor, and an X-ray sensor—are used simultaneously to image cells, aiming to answer questions about the mechanisms of ATP changes in mitochondria under the regulation of zinc ion efflux from lysosomes by TRPML1.

The application provides a miRNA imaging application method based on an endogenous glutathione activated DNA sensor for logic cascade signal amplification. The technology constructs a "local catalytic hairpin assembly DNA nanomachine", and triggers a cascade reaction through endogenous glutathione (GSH) and target miRNA-21: GSH cleaves the disulfide bond on the GH1 hairpin, so that the conformation is changed into a metastable state H1, and then hybridizes with miRNA-21 to start branch migration, releases miRNA-21 to circulate and catalyze H2 hairpin assembly, and realizes exponential amplification of signals. FAM and BHQ1 on the H2 hairpin are spatially separated due to double-stranded formation, and the fluorescence resonance energy transfer is released, so that a strong fluorescence signal positively related to the concentration of miRNA-21 is generated. The method has high sensitivity (the detection limit is 3.92 pM), high specificity and excellent stability, is suitable for high-fidelity imaging detection of miRNA in living cells, and provides an efficient tool for cancer diagnosis and treatment integration.