Preparation method of graphene DNA (deoxyribonucleic acid) sensor
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A graphene and sensor technology, applied in the field of graphene DNA sensor preparation, can solve problems such as limited samples for detection, and achieve the effect of good stability
Inactive Publication Date: 2014-03-12
JIANGSU UNIV
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[0002] Most of the existing graphene sensors are based on graphene oxide, which uses the property of graphene oxide to quench fluorescence to detect DNA sequences labeled with fluorescence at the end. This method can detect very limited samples and requires Fluorescent labeling still has certain limitations in terms of detection sensitivity and specificity
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[0017] The experimental methods described in the following examples, unless otherwise specified, are conventional methods; the materials, unless otherwise specified, can be obtained from commercial sources.
[0018] The graphene DNA sensor of the present invention is shown in Figure 1, S is the source, D is the drain, and the graphene surface 1 on the graphene transistor device connects DNA3 through the gold nanoparticle 2, thereby constitutes the graphene DNA sensor.
[0019] Concrete preparation steps are:
[0020] 1) Use chemical vapor deposition on copper foil to grow large-area continuous single-layer graphene; put copper foil in CVD (Chemical Vapor Deposition, chemical vapor deposition), pass hydrogen and methane, and react at 1050 ° C Afterwards, the hydrogen is cooled for storage.
[0021] 2) Soak the graphene grown on the copper foil in the gold nanoparticle solution; after growing the graphene on the copper foil in CVD, soak the copper foil in the 10nm-sized gold na...
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Abstract
The invention discloses a preparation method of a graphene DNA (deoxyribonucleic acid) sensor. The preparation method comprises the steps of 1) growing graphene; 2) connecting gold nanoparticles with the surface of graphene obtained in the step 1); 3) preparing a graphene transistor device from the obtained graphene with the surface connected with the gold nanoparticles; 4) preparing the graphene DNA sensor by connecting the gold nanoparticles on the surface of graphene with DNA.
Description
technical field [0001] A method for preparing a graphene DNA sensor belongs to methodological research in the field of biomedicine. Background technique [0002] Most of the existing graphene sensors are based on graphene oxide, which uses the property of graphene oxide to quench fluorescence to detect DNA sequences labeled with fluorescence at the end. This method can detect very limited samples and requires Fluorescent labeling still has certain limitations in terms of detection sensitivity and specificity. Li et al reported in Science in 2009 the use of chemical vapor deposition to prepare a major breakthrough in the preparation of large-scale, high-quality graphene films (Li, X.S., Cai, W.W., An, J.H., Kim, S., Nah, J. ., Yang, D.X., Piner, R., Velamakanni, A., Jung, I., Tutuc, E., Banerjee, S.K., Colombo, L., Ruoff, R.S., Large-Area Synthesis of High-Quality and Uniform Graphene Films on Copper Foils, Science, 2009,324:1312-1314.), this provides an opportunity for lar...
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