Vibrio DNA electrochemical sensor and preparation method and application thereof

A sensor and electrochemical technology, applied in the field of bioelectrochemical sensors, can solve the problems of poor overall dispersion and easy sedimentation of hybrids, and achieve the effects of enhancing immobilization ability, simple preparation method and wide linear range.

Active Publication Date: 2019-01-04
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the composite solution can solve the problems of GR sheet stacking and LDHNS aggregation, the composite hybrid is easy t

Method used

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  • Vibrio DNA electrochemical sensor and preparation method and application thereof
  • Vibrio DNA electrochemical sensor and preparation method and application thereof
  • Vibrio DNA electrochemical sensor and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0029] (1) Preparation of IL-GR-LDHNS complex

[0030] A certain amount of GO was added to 100 mL of formamide containing 200 mg IL, and ultrasonically dispersed for 2 h to obtain an IL-GO dispersion with a GO concentration of 1.0 mg / mL; MgCl was added to it at a molar ratio of 2:1 2 ·6H 2 O and AlCl 3 9H 2 O, make the total metal ion concentration 0.03mol / L, stir for 1h to completely dissolve the metal salt, and slowly titrate with the formamide solution of sodium hydroxide with a concentration of 0.5mol / L until the pH of the reaction solution is about 9.0 to 10.0, stirred at room temperature for 12 hours, then aged at 60°C for 12 hours, centrifuged the reaction solution at 8000rpm for 10 minutes, washed with ethanol and deionized water for 3 times, and dried naturally at room temperature to obtain ionic liquid functionalized graphene - Hydrotalcite-like nanosheet composites, denoted as IL-GR-LDHNS;

[0031] (2) Preparation of IL-GR-LDHNS modified glassy carbon electrode ...

Embodiment 2

[0036] (1) Preparation of IL-GR-LDHNS complex

[0037] A certain amount of GO was added to 100 mL of formamide containing 200 mg IL, and ultrasonically dispersed for 2 h to obtain an IL-GO dispersion with a GO concentration of 0.5 mg / mL; MgCl was added to it at a molar ratio of 2:1 2 ·6H 2 O and AlCl 3 9H 2 O, make the total metal ion concentration 0.03mol / L, stir for 1h to completely dissolve the metal salt, and slowly titrate with the formamide solution of sodium hydroxide with a concentration of 0.5mol / L until the pH of the reaction solution is about 9.0~10.0, stirred at room temperature for 12 hours, then aged at 70°C for 12 hours, centrifuged the reaction solution at 8000rpm for 10 minutes, washed with ethanol and deionized water for 3 times, and dried naturally at room temperature to obtain ionic liquid functionalized graphene - Hydrotalcite-like nanosheet composites, denoted as IL-GR-LDHNS;

[0038] (2) Preparation of IL-GR-LDHNS modified glassy carbon electrode

...

Embodiment 3

[0043] (1) Preparation of IL-GR-LDHNS complex

[0044] A certain amount of GO was added to 100 mL of formamide containing 200 mg IL, and ultrasonically dispersed for 2 h to obtain an IL-GO dispersion with a GO concentration of 1.5 mg / mL; MgCl was added to it at a molar ratio of 2:1 2 ·6H 2 O and AlCl 3 9H 2 O, make the total metal ion concentration 0.03mol / L, stir for 1h to completely dissolve the metal salt, and slowly titrate with the formamide solution of sodium hydroxide with a concentration of 0.5mol / L until the pH of the reaction solution is about 9.0~10.0, stirred at room temperature for 12 hours, then aged at 60°C for 12 hours, centrifuged the reaction solution at 8000 rpm for 10 minutes, washed with ethanol and deionized water for 3 times, and dried naturally at room temperature to obtain ionic liquid functionalized graphene - Hydrotalcite-like nanosheet composites, denoted as IL-GR-LDHNS;

[0045] (2) Preparation of IL-GR-LDHNS modified glassy carbon electrode

...

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Abstract

The invention discloses a vibrio DNA electrochemical sensor and a preparation method and application thereof. The method comprises the steps that graphene oxide is stripped in a N,N-dimethyl formamidesolution containing amino ion liquid, and ion liquid functional graphene is prepared; then, hydrotalcite nano-sheets are prepared in the dispersion solution, an ion liquid functional graphene-like-hydrotalcite nano-sheets composite is prepared, a drip coating method is adopted for preparing a glass carbon electrode modified by the compound, a vibrio probe ssDNA is fixed to the surface of the modified electrode through a biologic connecting agent, and the vibrio DNA sensor is prepared. The DNA sensor has the good sensitivity and selectivity, the low detection limit and the side linear range. The preparation method comprises the steps of preparing the modified electrode material, preparing the electrochemical auxiliary hemolysis vibrio DNA sensor, hybridizing the electrochemical auxiliary hemolysis vibrio DNA sensor with target DNA, and performing sensor electrochemical signal detection.

Description

Technical field: [0001] The invention belongs to the technical field of bioelectrochemical sensors, and in particular relates to a Vibrio DNA electrochemical sensor and a preparation method thereof, as well as specific DNA for highly sensitive detection of pathogenic Vibrio parahaemolyticus. Background technique: [0002] Marine Vibrio is a kind of rod-shaped or arc-shaped Gram-negative bacteria with strong activity, which is widely distributed in brackish water, coastal areas, estuaries, marine water bodies, sediments and marine organisms, and is distributed in the global estuary environment. Gram-negative bacteria that can cause acute gastroenteritis in humans when raw, undercooked, or mishandled seafood is consumed. Among them, Vibrio parahaemolyticus is the most harmful. The study found that the tlh gene was found in all strains of Vibrio parahaemolyticus, so it was considered as a useful indicator for detecting total Vibrio parahaemolyticus. In recent years, DNA electr...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/333
CPCG01N27/308G01N27/333
Inventor 詹天荣李凯利田夏
Owner QINGDAO UNIV OF SCI & TECH
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