75 results about "Chemical signaling" patented technology

An electrochemical detection system which specifically detects selected nucleic acid segments is described. The system utilizes biological probes such as nucleic acid or peptide nucleic acid probes which are complementary to and specifically hybridize with selected nucleic acid segments in order to generate a measurable current when an amperometric potential is applied. The electrochemical signal can be quantified.

Methods and devices for determining the concentration of a constituent in a physiological sample are provided. The physiological sample is introduced into an electrochemical cell having a working and counter electrode. At least one electrochemical signal is measured based on a reaction taking place at the cell. The preliminary concentration of the constituent is then calculated from the electrochemical signal. This preliminary concentration is then multiplied by a hematocrit correction factor to obtain the constituent concentration in the sample, where the hematocrit correction factor is a function of the at least one electrochemical signal. The subject methods and devices are suited for use in the determination of a wide variety of analytes in a wide variety of samples, and are particularly suited for the determination of analytes in whole blood or derivatives thereof, where an analyte of particular interest is glucose.

The invention discloses an experimental apparatus for high-temperature high-pressure loop spray corrosion simulation and electrochemical testing, belongs to the electrochemical field, and is particularly suitable for simulation experiment of erosion corrosion caused by simulating the complex components such as elbow, tee and the like in petroleum and gas collection pipes, petroleum and gas conveying pipes and the like so as to convey the high-temperature high-pressure multi-phase fluid pipe components and in-situ electrochemical signal testing of the erosion corrosion of high-temperature high-pressure fluid under erosion conditions. The apparatus comprises a high pressure kettle, a circulating loop, a high-temperature high-pressure flow gauge, a vortex pump, a control box, a circulating condensation system, a three-electrode system, a signal conduction path, an external electrochemical work station and a high pressure gas bottle. The apparatus has the advantages of simulating the erosion corrosion condition of the fluid medium in the oil and gas pipeline well, testing the electrochemical signal of the working electrode under high-speed spray condition such as polar curve, AC impedance and the like and having important significance to the research of the erosion corrosion action, rule and mechanism of the high-temperature high-pressure fluid.

The invention discloses an implantable neural information dual-mode detection microelectrode array chip and a manufacturing method thereof, relating to a sensor technology. The implantable neural information dual-mode detection microelectrode array chip comprises a silicon probe base, a plurality of microelectrodes which are arranged in an array manner, a counter electrode, an electrochemical reference electrode, an electrophysiological reference electrode, leads, welding pads and an insulating layer. The implantable neural information dual-mode detection microelectrode array chip is manufactured by a microelectromechnical system (MEMS) process; the front end part of a silicon probe can be implanted into a nerve tissue in the body of an animal, is used for detecting a neuroelectrophysiological signal and a neurotransmitter electrochemical signal at the same time, and has a function of applying electrical stimulation to the nerve tissue. The implantable neural information dual-mode detection microelectrode array chip has integrated functions, and is applicable to in-situ and synchronous dual-mode detection on neural information and relevant researches after implantation into the body of the animal in emergency or for a long time.

The invention discloses an ex-vivo nerve information dual mode detection microelectrode array chip and a preparation method of the ex-vivo nerve information dual mode detection microelectrode array chip, relating to the sensor technology. The chip consists of an insulation substrate, a microelectrode array, a counter electrode, a reference electrode, an electrode lead wire and a contact point and a surface insulation layer and is processed and prepared by adopting the MEMS (micro electro mechanical system) technology, wherein a microelectrode is a sensitive unit which is directly contacted with ex-vivo nerve cells of an animal, can be used for synchronous detection of transmission modes of a neural electrophysiological signal and a nervous transmitter electrochemical signal by combining the counter electrode and the reference electrode and also has the function of applying electrophotoluminescence to cells. The ex-vivo nerve information dual mode detection microelectrode array chip has function integration, is simple in circuit interface, is convenient to use and is suitable for performing dual-mode detection of ex-vivo nerve information of animals and related research in laboratories.

Low-cost, non-toxic and fast immunoassay systems (immunosensors) and uses thereof as analytical and diagnostic tools for detecting an immune response in a subject are disclosed. The systems and methods disclosed are based on recording an electrochemical signal which is generated proportionally to an enzymatic cascade reaction (enzyme-channeling) upon detecting an analyte, and therefore can be used to determine the titer level of an antibody analyte in a liquid sample such as artificial media, serum or blood both qualitatively and quantitatively, in a one-step and separation free immunoassay. Systems and methods based on recording an electrochemical signal which is generated proportionally to an enzymatic cascade reaction (enzyme-channeling) upon detecting an analyte, which utilize a non-toxic secondary substrate such as acetaminophen are also disclosed.

An electrochemical detection system which specifically detects selected nucleic acid segments is described. The system utilizes biological probes such as nucleic acid or peptide nucleic acid probes which are complementary to and specifically hybridize with selected nucleic acid segments in order to generate a measurable current when an amperometric potential is applied. The electrochemical signal can be quantified.

Several methods may be used to exploit the natural physical variations of sensors, to generate cryptographic physically unclonable functions (PUF) that may strengthen the cybersecurity of microelectronic systems. One method comprises extracting a stream of bits from the calibration table of each sensor to generate reference patterns, called PUF challenges, which can be stored in secure servers. The authentication of the sensor is positive when the data streams that are generated on demand, called PUF responses, match the challenges. To prevent a malicious party from generating responses, instructions may be added as part of the PUF challenges to define which parts of the calibration tables are to be used for response generation. Another method is based on differential sensors, one of them having the calibration module disconnected. The response to a physical or chemical signal of such a sensor may then be used to authenticate a specific pair of sensors.

The invention provides a top-corrosion electrochemical testing device in high-temperature and high-pressure environments, belongs to the field of corrosive electrochemistry, and is suitable for top-corrosion simulation experiments and corrosion behavior mechanism research in high-temperature and high-pressure working conditions. The top-corrosion electrochemical testing device comprises a high-temperature and high-pressure reaction kettle, a heating and heat preservation system, a circulating condensate water tank refrigerating system, a three-electrode system, a signal conduction pathway, a control box, an external air supply system and a computer. The device has the advantages that actual high-temperature and high-pressure working conditions can be achieved; top-corrosion in-situ electrochemical signal testing can be carried out; and the method is of great significance on mechanism research on high-temperature and high-pressure top-corrosion behaviors and electrochemical reaction.

The invention provides a metal corrosion high-throughput characterization experimental apparatus based on an image, belonging to the technical field of metal electrochemical corrosion. The experimental apparatus comprises a high-throughput electrolytic tank, a metal electrode, an electrochemical signal acquisition device, an image signal acquisition device and a data processing device, wherein the high-throughput electrolytic tank is mounted on a testbed, the bottom of the high-throughput electrolytic tank is connected with the electrochemical signal acquisition device through a lead, the upper part of the high-throughput electrolytic tank is connected with the electrochemical signal acquisition device through a reference electrode, the image signal acquisition device is arranged above the high-throughput electrolytic tank, and both the image signal acquisition device and the electrochemical signal acquisition device are connected with the data processing device. The metal corrosion high-throughput characterization experimental apparatus is used for metal corrosion tests, synchronous reaction of a plurality of samples can be carried out at the same time, and meanwhile, the real-time acquisition of electrochemical signals and image signals is realized, so that repeated experiments are reduced, the correlation of experimental data is improved, and the accuracy and the efficiency of the metal corrosion tests are finally improved.

The invention discloses a preparation method of a magnetic molecular imprinting photonic crystal sensor for detecting melamine and belongs to the technical field of molecular imprinting photonic crystal and analysis detection. The product is obtained through preparation of oleic acid modified ferroferric oxide magnetic nanoparticles, preparation of melamine magnetic molecular imprinting nanoparticles and preparation of the magnetic molecular imprinting photonic crystal sensor. The melamine magnetic molecular imprinting nanoparticles are self-assembled to form a photonic crystal structure under the action of an external magnetic field, and a liquid-state molecular imprinting photonic crystal sensor is obtained. On a macro level, with increase of the concentration of melamine, the diffraction color of the sensor changes from weak purple to green, yellow and finally orange red. The preparation method has the advantages that the photonic crystals are assembled rapidly, simply and conveniently. The provided molecular imprinting photonic crystal sensor transmits a chemical signal of melamine concentration change directly through an optical signal of diffraction color change and realizes the purpose of naked eye detection.

The invention provides a water body toxicity detecting method and portable device based on microbial electrochemical signals. The method and the device are characterized in that a microbial fuel cell (MFC) technology is combined with an integrated circuit technology. The device comprises an MFC signal generator and a signal acquisition and alarm circuit. Anode materials of the MFC signal generator are subjected to electrochemical modification, and subjected to inoculation by using electricity-producing pure bacteria, so that the start-up time is shortened, and the signal stability of a baseline is enhanced. The signal acquisition and alarm circuit comprises an SCM (single chip microcomputer) minimum system, a power supply module, a liquid crystal display module and a sound-light alarm system. When a water body contains toxic substances, an output signal of the MFC signal generator deviates from the baseline, and when a deviation value exceeds a design threshold of the alarm circuit, the sound-light alarm system on the circuit is started to send the alarm. The device disclose by the invention is stable in signal, simple in design, strong in portability, and applicable to various water quality detection occasions.

The invention discloses a vibrio DNA electrochemical sensor and a preparation method and application thereof. The method comprises the steps that graphene oxide is stripped in a N,N-dimethyl formamidesolution containing amino ion liquid, and ion liquid functional graphene is prepared; then, hydrotalcite nano-sheets are prepared in the dispersion solution, an ion liquid functional graphene-like-hydrotalcite nano-sheets composite is prepared, a drip coating method is adopted for preparing a glass carbon electrode modified by the compound, a vibrio probe ssDNA is fixed to the surface of the modified electrode through a biologic connecting agent, and the vibrio DNA sensor is prepared. The DNA sensor has the good sensitivity and selectivity, the low detection limit and the side linear range. The preparation method comprises the steps of preparing the modified electrode material, preparing the electrochemical auxiliary hemolysis vibrio DNA sensor, hybridizing the electrochemical auxiliary hemolysis vibrio DNA sensor with target DNA, and performing sensor electrochemical signal detection.

The invention provides an analysis method for electrochemical detection of lithium precipitation and a corresponding module device thereof, and the method comprises the following steps: charging a to-be-detected battery, then carrying out standing treatment, and collecting an electrochemical signal of the battery; characterizing whether the sample battery generates lithium precipitation or not inthe previous charging process by analyzing the collected electrochemical signal, and grading the lithium precipitation degree of the sample battery; and if the semi-quantitative analysis needs to be carried out, carrying out discharge treatment on the lithium-separated battery and collecting an electrochemical signal. The battery semi-quantitative lithium precipitation amount is obtained by analyzing the discharge and the previous charge electrochemical signal. According to the method, the defects of complicated operation, low precision, toxicity, harm and the like of the traditional method for analyzing lithium precipitation by disassembling the battery are overcome, and the lithium precipitation of the battery is quickly, accurately and quantitatively analyzed by utilizing an electrochemical means and a modular device.

The invention discloses a DNA fragment separating colony of rice etiological agent special induced promoter POsDR3, functional checking and appliance in plant gene engineering technical domain, which is characterized by the following: generating specific answer reaction for invading rice of pathogenic bacteria; developing answer reaction for inducement of diverse hormones (abscisic acid, ethene, activol) and chemical signal molecule (jasmine acid, salicylic acid); quick-intensifying expression of POsDR3 promoter controlling gene with pathogenic bacteria invasion and chemical signal molecular treatment. The POsDR3 promoter can intensify resistance of rice, which does not make rice generate excess protein.

The invention discloses a wireless wearable neural signal detectiondevice and system. The device includes a flexible microelectrode array and a wearable detector, wherein the flexible microelectrode array can be implanted into nervous nuclei in the brain, and is applied to synchronous detection of neural electrophysiological signals and neural electrochemical signals, the wearable detector is usedfor receiving the neural electrophysiological signals and neural electrochemical signals which are output by the flexible microelectrode array, performing conditioning analysis, analog-to-digital conversion and compression on the neural electrophysiological signals and neural electrochemical signals and sending the compressed signals in a wireless mode. Unrestricted free movement and behavior ofanimals are facilitated in the research, and the problem of difficult movement of the animals duringcontinuous monitor of neural information in the research of behavioral neural mechanisms of the animals can besolved.

The invention discloses an electrochemical sensing detection method of protein O-GlcNAc glycosyltransferase activity. The electrochemical sensing detection method comprises the following steps that a membrane of substrate polypeptide molecules of OGT is formed on the surface of an electrode; the obtained electrode fixed with the membrane of the polypeptide molecules on the surface and a glycosylation reaction fluid are incubated through OGT catalysis to obtain an electrode fixed with the membrane of the glycosylated polypeptide molecules on the surface; the electrode fixed with the membrane of the polypeptide molecules on the surface and the electrode fixed with the membrane of the glycosylated polypeptide molecules on the surface are sequentially subjected to the treatment in the steps 1-3 as follows: 1, the electrodes are put in a protease reaction fluid for hydrolysis; 2, the electrodes are cleaned with water; 3, a tyrosine residue serves as an electrochemical signal probe, and the electrodes perform electrochemical oxidation reaction; tyrosine residue electrocatalytic oxidized currents in the obtained systems are respectively determined, and OGT activity can be quantitively analyzed according to the obtained catalyzed and oxidized currents. By adopting the method, simple, quick and non-marked OGT activity detection is achieved through an electrochemical biosensor.

The invention discloses a construction method for a corrosion degree and mechanical property degradation law model based on an electrochemical signal of a stressed steel bar. The construction method comprises the steps that a to-be-processed steel bar is installed on a stress frame designed in the early stage, and a certain tensile stress is applied to the steel bar by an assistor; then under thestate with the tensile stress, the to-be-processed steel bar is put into a corrosive fluid for corrosion, and an electrochemical signal is collected; based on the collected electrochemical signal, combined with the existing calculation model of a non-stressed steel bar, two correction factors, namely DZ and HBH are added, the corrosion degree of the stressed steel bar is calculated, wherein DZ isthe correction coefficient after applying force, and HBH is the correction coefficient of the original crack state; and mechanical property indexes are measured according to different corrosion degrees, and a mechanical property degradation law DZ-HBH model based on the electrochemical signal is constructed. The original crack propagation mechanism of the steel bar itself may be correctly predicted, and a reasonable explanation on the instantaneous fracture mechanism is given.

The invention belongs to the optical microscope instrument manufacturing technology and electrochemical imaging technology field and especially relates to an electrochemical imaging system and methodbased on a graphene adjustable light scattering property. The system comprises a graphene electrolytic cell system, a microscope system, an imaging module and an image processing module. The grapheneelectrolytic cell system uses graphene as a working electrode. The microscope system and the imaging module are arranged on a scattered light path of the graphene electrolytic cell system. The image processing module extracts a picture obtained by the imaging module, and carries out operation processing on picture scattered light intensity to obtain the picture representing local surface Faraday and non-faradaic current densities of a target sample so as to realize electrochemical imaging. By using the electrochemical imaging method based on the graphene adjustable light scattering property, local surface electrochemical signals of the multiple target samples in a field of view can be detected simultaneously, sensitivity of current collection is reduced from a nanoampere level in the priorart to an angstrom ampere level by a matched scattering imaging system, and a spatial resolution is significantly improved.