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Micro-extraction/micro electrode bifunctional probe and preparation method and application thereof

A micro-electrode, dual-function technology, applied in the field of micro-extraction/micro-electrode dual-function probes and its preparation, can solve problems such as complex operation, inability to achieve multi-channel precise positioning, real-time quantitative analysis, etc.

Active Publication Date: 2018-06-22
YANBIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although these methods are relatively mature, there are many shortcomings, the operation is complicated, and it is impossible to realize multi-channel precise positioning and real-time quantitative analysis.

Method used

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  • Micro-extraction/micro electrode bifunctional probe and preparation method and application thereof
  • Micro-extraction/micro electrode bifunctional probe and preparation method and application thereof
  • Micro-extraction/micro electrode bifunctional probe and preparation method and application thereof

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preparation example Construction

[0026] The invention provides a method for preparing a micro-extraction / micro-electrode dual-function probe, comprising the following steps:

[0027] (1) Provide carbon nanofiber / carbon fiber material, the carbon nanofiber grows on the surface of carbon fiber, has three-dimensional three-dimensional structure of bionic caterpillar space;

[0028] (2) One end of the carbon fiber in the carbon nanofiber / carbon fiber material obtained in the step (1) is subjected to surface chemical activation treatment to obtain activated carbon nanofiber / carbon fiber;

[0029] (3) Fe 3 o 4 The nanoparticle dispersion is coated on the activated carbon nanofiber / carbon fiber that described step (2) obtains, obtains Fe 3 o 4 / carbon nanofiber / carbon fiber;

[0030] (4) the carbon nanofiber / carbon fiber obtained by one described step (1) and the Fe obtained by one described step (3) 3 o 4 / carbon nanofibers / carbon fibers are loaded into the dialysis membrane to obtain micro-extraction / m...

Embodiment 1

[0047] Preparation of carbon nanofibers / carbon fibers

[0048] The chemical vapor deposition method disclosed in CN106353439A is adopted to prepare carbon nanofibers / carbon fibers, the diameter of carbon nanofibers is 20nm, and the diameter of carbon fibers is 5 μm, and the structural representation of the prepared carbon nanofibers / carbon fibers is as follows figure 1 ,Depend on figure 1 It can be seen that in the prepared carbon nanofiber / carbon fiber, the carbon nanofiber is decorated on the carbon fiber, and has a three-dimensional three-dimensional structure of a bionic caterpillar.

[0049] Fe 3 o 4 / Carbon Nanofiber / Carbon Fiber (Fe 3 o 4 / CNFs / CF) preparation

[0050] figure 2 For the preparation of Fe 3 o 4 / Carbon Nanofibers / Flow chart of carbon fibers. Fe was first prepared from iron precursors 3 o 4 Magnetic nanoparticles: FeCl 3 ·6H 2 O and FeCl 2 4H 2 O and concentrated hydrochloric acid were dissolved in pre-N 2 Deoxidized deionized water; un...

Embodiment 2

[0059] Preparation of carbon nanofibers / carbon fibers

[0060] The chemical vapor deposition method disclosed in CN106353439A is used to prepare carbon nanofiber / carbon fiber, the diameter of carbon nanofiber is 50nm, and the diameter of carbon fiber is 7 μ m, and the carbon nanofiber in the prepared carbon nanofiber / carbon fiber is modified on the carbon fiber, with bionic hair The three-dimensional three-dimensional structure of insect space.

[0061] Fe 3 o 4 / Carbon Nanofiber / Carbon Fiber (Fe 3 o 4 / CNFs / CF) preparation

[0062] FeCl 3 ·6H 2 O and FeCl 2 4H 2 O and concentrated hydrochloric acid were dissolved in pre-N 2 Deoxidized deionized water; under the condition of 80℃ water bath and mechanical stirring, add the above solution dropwise to N 2 In the protected alkaline aqueous solution, after the dropwise addition, continue to feed N 2 , while insulated and stirred; after the reaction, the ferric oxide nanoparticles (diameter less than 20nm) were separated us...

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Abstract

The invention provides a preparation method of a micro-extraction / micro electrode bifunctional probe and belongs to the field of biological signal molecule detection. In the micro-extraction / micro electrode bifunctional probe, a carbon nanofiber (CNFs) / carbon fiber (CF) has high adsorption activity, and micro-extraction and micro electrodes are integrated. As magnetic nanoparticles Fe3O4 are modified on the surface of CNFs, not only is the defect that the magnetic nanoparticles Fe3O4 are liable to aggregate overcome, but also the magnetic response property of the CF is improved. By regulatingand controlling movement routes of Fe3O4 / CNFs / CF through magnetic fields, magnetic navigation is achieved, and through electrochemical signal variations of Fe3O4 / CNFs / CF, precise positioning of signalmolecule release sites is achieved; due to bidirectional regulation and control of magnetic fields and electric signals, a novel detection method for cell signal molecule positioning quantitative analysis is provided, and essential theoretic instructions can be provided to design novel efficient cell analyzers later.

Description

technical field [0001] The invention relates to the technical field of biological signal molecule detection, in particular to a micro-extraction / micro-electrode dual-function probe, a preparation method and application thereof. Background technique [0002] In recent years, accurate disease classification and diagnosis through precise regulation of intracellular and extracellular signal transduction, and formulation of individualized disease prevention and treatment programs have quietly become central issues in life science research, and the study of "synergy of multi-pathway signaling molecules" It is the most effective prerequisite for precise regulation of cell signal transduction systems. Since the cell signal transduction process requires multiple pathways to complete (synergism or antagonism), understanding the composition of the signal transduction system and the synergy of multi-pathway signaling molecules is of great significance for in-depth understanding of life ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
CPCG01N27/26
Inventor 李东浩邹依霖孟龙月
Owner YANBIAN UNIV
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