35results about How to "Easy self-assembly" patented technology

The invention relates to a preparation method and application of a self-assembled biomimetic phospholipids polymer membrane. The preparation steps comprise (1) monomer preparation and (2) oligomer preparation. Connect the hydrophilic phospholipids groups and their derivatives with the dual bonds hydrophobic aliphatic hydrocarbons and synthesize a self-assembled microcosmic heterogeneous phase similar to the biological membrane. The invention can imitate PH values of different organs by adjusting the PH value of the penetrant liquid, or can regulate the stability and flexibility of the phospholipid polymer by controlling the polymer molecular size, the hydrophobic chain length and the phospholipid content. The membrane coverage and thickness can be adjusted according to requirements while the single or double layer membrane can be made according to the molecule's PH value change with the environment. The preparation method is fast and easy, low cost and can be kept for a long time, helps to accelerate the new medicine discovery and development. The invention is applied to bring various polarized phospholipids and their derivatives such as lecithin and cephalin on the hydrophobic chain, and set up the simulation cell membrane model with different targets.

The invention discloses a preparation method of a homogeneous cyclic peptide Cyclo-(Ala)4. The preparation method comprises the steps: (1) allowing resin to react and be connected with alanine with aprotecting group to form resin connected with alanine, (2) conducting continuous condensation on alanine connected with the resin and alanine with the protecting group to form resin connected with a linear chain peptide, (3) cutting the linear chain peptide from the resin for end-to-end cyclization to form a cyclic peptide crude product, and (4) purifying and storing the cyclic peptide. Cyclo-(Ala)4 prepared by the method is in a homogeneous cyclic peptide structure formed by single amino acid, has a structure extremely similar to that of a crown ether compound; molecules having good regularity and multidirectional symmetry in structure can be self-assembled into an ion channel or a nano tube more easily as a drug carrier, a membrane channel, a molecular element and the like; no free aminogroup or carboxyl group is available in the molecules of the cyclic peptide, so that the cyclic peptide has extremely good liposolubility and biological in-vivo stability; at the same time, the method is reasonable in technology and simple in operation; the synthesis efficiency is higher.

The invention provides glycosyl polyethers, a preparation method thereof and use thereof. In the method, macromolecular glycosyl polyethers which have a molecular weight ranging from several hundred to more than 100,000 and have a structure below are prepared by starting from common glycosyl donors and by a method of using the glycosyls as starting ends, bonding the glycosyls with the open-loop long chains of alkylene oxide at anomeric positions and using common nucleopilic reagents such as hydroxys, alkoxys, aminos, nitrines and halogen atoms as capping ends. As the mechanical performance and plasticity of the glycosyl polyether compounds can be controlled conveniently by regulating the length of the polyether chain, the glycosyl polyether compounds can be used as medical materials, important materials for synthesizing polyurethane, thermoplastic polyester-polyamide elastic materials and precursors for preparing glyconanoparticles.

The invention discloses a preparation method of homocyclic peptide Cyclo-[(Asp)5-Gly]. The method includes: (1) connection of resin with protective group equipped aspartic acid to form aspartic acid connected resin; (2) sequential condensation of the aspartic acid connected to resin with the protective group equipped aspartic acid and allylglycine to form linear peptide connected resin; (3) cutting of the linear peptide off the resin and end-to-end cyclization to obtain a cyclic peptide crude product; and (4) purification and preservation of cyclic peptide. The Cyclo-[(Asp)5-Gly] prepared by the invention is a homocyclic peptide structure, has a molecular structure with better regularity, is easier for self-assembly into an ion channel or nanotube, thus serving as a drug carrier, membrane channel, molecular device and the like. The five carboxyl functional groups contained on a side chain endow the product with certain water solubility and bioactivity, also an allyl functional group contained on the side chain endows the product with better reaction activity, so that the product can be used as a precursor reagent for high efficient click reaction. At the same time, the method has the advantages of reasonable process, simple operation, and high synthesis efficiency.

The invention relates to a laser nano material processing method and a processing system. The processing system includes a laser, a beam shaping module, a target module and a monitoring module, and a target seat located at the liquid interface is set, so that the laser enters and ablates different targets in different environments from the liquid interface. The interface is very close, so it can be easily self-assembled at the interface, and the target can be self-assembled at the interface to obtain a single-layer film. This preparation method has good controllability, and is more energy-saving, environmentally friendly and pollution-free than the chemical preparation method; use Transmittance monitoring indirectly monitors the self-assembly process of the liquid interface, with good accuracy and low monitoring cost; the device and method of the present invention are suitable for preparing nanometer single-layer film structures of various components, and can be widely used in various scientific research laboratories.

The invention relates to self-assembly systems formed by diphenol compounds and 2-formylbenzeneboronic acid and an application of the self-assembly systems as a fluorescence probe. According to the invention, two diphenol compounds respectively form the self-assembly systems with the 2-formylbenzeneboronic acid, the self-assembly systems can be taken as the fluorescence probe, the self-assembly systems are formed by adding the diphenol compounds and 2-formylbenzeneboronic acid in a phosphoric acid buffer, quantitative determination is carried out on free radical hypochloric acid and peroxynitrite, the probe has the advantages that the self fluorescence is weak, the fluorescence is obviously enhanced after effecting with a to-be-measured object, and compared with other detection free radical fluorescence quenching-type probes, the fluorescence probe has the characteristics of higher signal to noise ratio, rapidity, sensitivity, simpleness, and high efficiency.

The invention discloses a preparation method of a thermo-sensitive fluorescent polymer. The preparation method comprises the following steps: (1) synthesizing an NMA (N-alpha-naphthyl methacrylamide) monomer; (2) synthesizing a P(St-co-NMA)m (poly(styrene-co-NMA) macromolecular chain transfer agent; (3) synthesizing P(St-co-NMA)m-b-PENGMAn (P(St-co-NMA)m-b-poly(polyethylene glycol methacrylate)), namely the thermo-sensitive fluorescent polymer. According to the preparation method, the prepared nanoparticles simultaneously have thermosensitivity and fluorescence, and a series of fluorescent nanoparticles with different particle sizes can be prepared by changing external conditions.

The invention discloses phosphorylcholine polyethylene glycol modified chitosan and a preparation method thereof. The number-average molecular weight of the phosphorylcholine polyethylene glycol modified chitosan is 0.13 to 0.41 million, and the chemical structural formula of the phosphorylcholine polyethylene glycol modified chitosan is as shown in the description, wherein PC is as shown in the description, and n is equal to 4 to 50. The preparation method comprises the step of adopting chitosan and acrylate-terminated phosphorylcholine polyethylene glycol to carry out free radical polymerization so as to obtain the phosphorylcholine polyethylene glycol modified chitosan. The phosphorylcholine polyethylene glycol is introduced onto the chitosan, other reagents do not need to be introduced, a nanoparticle structure with a chitosan chain segment as a core and the phosphorylcholine polyethylene glycol on the surface can be self-assembled in water, meanwhile, the introduced phosphorylcholine polyethylene glycol chain segment has good water solubility, biocompatibility and flexibility, and phosphorylcholine radical is positioned at the tail end of the flexible polyethylene glycol, so that a simulated cell outer layer membrane structure is self-assembled more easily.

The invention discloses a method for manufacturing a surface plasmon resonator chip based on lysine modification and mucoprotein modification. The chip comprises a BK7 glass sheet substrate, the BK7 glass sheet substrate is plated with a chromium layer of 2 nm to 3 nm and a gold film layer of 47 nm to 48 nm, amphipathic mucoprotein is connected to the surface of the gold film through hydrophobic groups of a protein framework, carboxyl groups of sugar residues on mucoprotein side chains are activated through 1-(3-dimethylamino propyl)-3-EDAC and N-hydroxysuccinimide and used for being coupled with lysine, and therefore the mucoprotein surface plasmon resonator chip based on lysine modification is obtained. The chip manufactured through the method has the high antifouling property in a single protein system and a complex system with the pH equal to 7.4 and can be easily assembled on various surfaces, and a stable and regular monomolecular layer is formed. The chip has the excellent antifouling property and high stability, the preparation method is relatively easy and convenient, and high feasibility and universality are achieved.

The invention discloses a preparation method of homocyclic peptide Cyclo-[(Asn)5-Cys]. The method includes: (1) connection of resin with protective group equipped asparagine to form asparagine connected resin; (2) sequential condensation of the asparagine connected to resin with the protective group equipped asparagine and cysteine to form linear peptide connected resin; (3) cutting of the linear peptide off the resin and end-to-end cyclization to obtain a cyclic peptide crude product; and (4) purification and preservation of cyclic peptide. The Cyclo-[(Asn)5-Cys] provided by the invention is a homocyclic peptide structure, has a similar structure to crown ether compounds, and compared with the heterocyclic peptide, has better regularity in terms of molecule structure, is easier for self-assembly into an ion channel or nanotube, thus serving as a drug carrier, membrane channel, molecular device and the like. The five amino functional groups contained on a side chain endow the product with certain water solubility and bioactivity, and a mercapto functional group contained on the side chain endows the product with better reaction activity, so that the product can be used as a precursor reagent for high efficient click reaction.