124 results about "Nanocages" patented technology

A method for mass production of high-quality hollow carbon nanocages comprises the following steps: 1) light magnesium carbonate or magnesium carbonate is added into a reaction tube and spread evenly, the tube is put into a tubular furnace, air is pumped out of the tube, inert gas such as N2 and Ar is injected; the reaction temperature is increased to 670 DEG C-900 DEG C, (C) source vapor is introduced, and reaction is carried out for 5 to 240 minutes under the protection of 10-500sccm inert gas atmosphere; the (C) source gas is led to the reaction area of the tubular furnace by the inert gas flow, carbonized on the surfaces of nano particles generated in situ, and covered to form a MgO@C structure; after the reaction, the temperature inside the reaction tube drops to the room temperature under the protection of the inert gas; 2) powder is collected from the reaction tube, dipped in sufficient hydrochloric acid or sulphuric acid for 5 to 720 minutes to remove the kernel of MgO, filtered, washed to neutrality by using deionized water, and dried to obtain the hollow carbon nanocages; and 3) the magnesium salt filtrate is recycled. The nanocages prepared by the method have high purity, and the price for the precursor is low, thereby facilitating recovery and reuse.

A process for preparing a lot of hollow carbone nanocages includes such steps as proportionally mixing metallic carbonyl liquid with low-carbon organic liquid to obtain reaction solution, heating reactor while introducing inertial gas to it, pumping said reaction solution into the reactor to obtain solid carbon nanocages, putting them in pure nitric acid or the mixture of nitric acid and distilled water, ultrasonic oscillating, heating, reflux cooling, diluting by distilled water, laying aside for deposition, and baking the deposited substance.

The invention provides a preparation method of a hollow gold nanometer cage, comprising the following steps: dissolving chloroauric acid, hexamine, and a protecting agent in water to prepare a growing liquid; getting the growing liquid and adding in a reducing agent and gold nanometer particle and mixing well to prepare gold seed solution; getting the growing liquid, adding in silver salt and the reducing agent, mixing well to prepare a mixed solution; adding the gold seed solution in the mixed solution, standing and reacting to prepare the hollow gold nanometer cage. The method provided by the invention directly enables the gold nanometer particle to grow into the hollow gold nanometer cage in water phase at normal temperature; besides, the process is simple, the reaction is easy to control, and the efficiency is high. Simultaneously, the preparation method has no need of performing post treatment to the product, thereby reducing the use amount of the reagent, reducing the production cost and simplifying the operation steps.

The invention relates to a metal-organic framework crystalline material with a nano-cage structure, in particular to a polyoxometalate (POM)-based metal-organic frameworks (POMOFs) crystalline material with a nano-cage structure and a preparation method and application thereof and aims to solve the problems in the prior art that a POMOFs crystalline material with a nano-cage structure is high in synthesis difficulty and poor in rhodamine B organic dye degradation effect. The POMOFs crystalline material has the chemical formula of Ag10(tta)4(H2O)4(SiW<IV>10W<V>2O40), the tetragonal system, and the space groups of I-4m2, alpha=90, beta=90, gamma=90 and Z=2. The preparation method comprises the following steps: dissolving silicotungstic acid, silver nitrate, tetrazole and a mineralizer in distilled water; regulating the pH value; carrying out a reaction for 3 days at 160 DEG C. The POMOFs crystalline material with the nano-cage structure can be obtained.

The present invention relates to gold nanocages containing magnetic nanoparticles and a preparation method thereof. More specifically, relates to hollow-type gold nanocage particles, which contain iron oxide nanoparticles having a magnetic property and have an optical property of strongly absorbing or scattering light in the near-infrared (NIR) region, as well as a preparation method thereof. Due to their optical property and magnetic property, the magnetic nanoparticle-containing gold nanocages can be used in various applications, including analysis in a turbid medium with light, cancer therapy or biomolecular manipulation using light, contrast agents for magnetic resonance imaging, magnetic hyperthermia treatment and drug delivery guide, etc.

The present invention provides a simple method of mass producing high purity carbon nanocages. The method of preparing carbon nanocages comprises introducing an inert gas into a reactor equipped with a spray nozzle and raising the temperature of the reactor to a pyrolysis temperature of a catalyst compound. The method further comprises spraying a reactant including the catalyst compound and a carbon-containing compound into the reactor through the spray nozzle, wherein the ratio of a space velocity of the inert gas to a space velocity of the reactant is 100 or more.

The invention provides a process for producing DNA nanocages, characterized by comprising a step of two-dimensionally assembling three types of oligonucleotides by hybridization to form tridirectionally branched double-strand DNA having self-complementary chains in the terminals, and a step of three-dimensionally self-organizing the resulting tridirectionally branched double-strand DNAs so as to consume all of the self-complementary terminals. Since the DNA nanocages according to the invention can easily be formed from DNAs by one-step procedure and include nanoparticles in the interior, the DNA nanocages are significantly effective for the development of functional materials using DNAs.

The invention discloses a preparation method of a photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system. The preparation method comprises the following steps of: 1) mixing a gold nanocage with a high-temperature ligand, stirring and incubating the mixture and performing centrifugation to obtain a high-temperature ligand-modified gold nanocage, wherein the high-temperature ligand is a chain-like temperature-sensitive polymer containing a disulfide bond, and the phase change temperature of the high-temperature ligand is 39-50 DEG C; 2) dispersing the high-temperature ligand-modified gold nanocage obtained in the step 1) in an ammonium sulfate solution, stirring and centrifugalizing the mixture, dispersing a solid with an aqueous solution of adriamycin, and leaving the mixture to stand in a dark place to obtain a drug-carrying gold nanocage; and 3) mixing the drug-carrying gold nanocage obtained on the step 2) with a low-temperature ligand, and stirring and incubating the mixture and performing centrifugation to obtain the photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system, wherein the low-temperature ligand is a chain-like temperature-sensitive polymer containing a disulfide bond, and the phase change temperature of the low-temperature ligand is 26-35 DEG C.

The invention belongs to the technical field of nano material production, and relates to a preparing method of hollow magnetic carbon nanospheres with MOFs growing inside in a confinement mode. The preparing method includes the steps that FeCl3.6H2O, ethylene glycol, ethyl alcohol and sodium acetate are mixed and subjected to hydrothermal reaction to obtain Fe3O4 spheres, the Fe3O4 spheres, ethyl alcohol, deionized water and ammonia water are mixed to obtain Fe3O4 sphere mixed solution, a silicon source is added dropwises into the Fe3O4 sphere mixed solution to react, then resorcinol and formaldehyde are added to react so as to obtain Fe3O4@SiO2@ resorcinol-formaldehyde resin spheres, Fe3O4@SiO2@C spheres are obtained after calcining under argon and etched by sodium hydroxide water solution to obtain magnetic carbon nanocages; finally the magnetic carbon nanocages react with metallic nitrate and 2-methylimidazole to obtain the hollow magnetic carbon nanospheres which are uniform in size and are distributed uniformly, the morphology of the hollow magnetic carbon nanospheres is controlled well, and the MOFs of the hollow magnetic carbon nanospheres grow inside in the confinement mode. The process is simple, and the materials needed in the reaction process are low in toxicity and harmless.

The invention discloses a production method and an application of an electrode modified with gold nano-cage and myoglobin. The production method comprises the following steps: taking graphite powder and an ionic liquid HPPF6 according to a mass ratio of (1.5-2.5):1, placing the graphite powder, the ionic liquid and liquid paraffin in a mortar, carrying out uniform grinding to obtain a carbon paste, filling a glass electrode tube with the carbon pate, and inserting a copper wire as a lead wire to obtain a carbon ionic liquid electrode CILE; dispensing 6-10 [mu]L of a AuNCs solution on the surface of the CILE, and naturally drying the CILE in a dark place under room temperature conditions to obtain an AuNCs / CILE electrode; dispensing 6-10 [mu]L of a 10-20 mg mL<-1> Mb solution on the surfaceof the AuNCs / CILE electrode, and naturally drying the AuNCs / CILE electrode in a dark place at room temperature to obtain a Mb / AuNCs / CILE electrode; and dispensing 4-8 [mu]L of a 0.3-0.7% Nafion ethanol solution on the surface of the Mb / AuNCs / CILE electrode, and drying the Mb / AuNCs / CILE electrode in a dark place at room temperature to obtain the Nafion / Mb / AuNCs / CILE electrode. The modified electrode has a good electrocatalytic reduction effect on trichloroacetic acid, sodium nitrite and hydrogen peroxide, has the advantages of wide linear range, low detection limit and high sensitivity, and can be well applied to the detection of the content of above three substances in an object.

The invention belongs to the field of high polymer chemistry and biomedical engineering, and particularly relates to a multi-functional stimuli sensitive polymer-gold nanocage carrier and a preparation method thereof. The multi-functional carrier has a nucleus-shell-crown structure formed by a nucleus, a shell and a crown; the nucleus is formed by polymeric micelles, the shell is formed by gold nanocages, and the crown is formed by methoxy polyethylene glycol-branched polyethylenimine-lipoic acid; and the nano-carrier has a good photothermal conversion effect on the condition of 808 nm. The carrier has low toxicity for cells and can bear not only drugs but also genes or nucleic acids, thereby achieving the integration of carrier positioning, chemotherapy, thermal therapy and gene therapy and having a wide application value.

The invention relates to the field of water treatment, and discloses a method for treating an antiepileptic drug in water by utilizing MnxCo3-xO4 nanocages for activating monoperoxysulfate; the method particularly includes the following steps: one, mixing monoperoxysulfate with an antiepileptic drug-containing aqueous solution; two, adjusting a reaction pH; three, preparing the MnxCo3-xO4 nanocages; four, adding the MnxCo3-xO4 nanocages; five, recycling the MnxCo3-xO4 nanocages, and thus completing the method for treating the antiepileptic drug in the water by utilizing the MnxCo3-xO4 nanocages for activating monoperoxysulfate. The removal effect of the typical antiepileptic drug carbamazepine in water by using the method is obvious, the removal rate reaches 95%-99%, a catalytic material can be recovered and reused through centrifugation and separation, and the cost is saved.

The invention provides a preparation method of a porous FexCo3-xO4 nanocage, the nanocage and application thereof. In a PVP solution, the morphology of Co-Co PBAs can be effectively modulated by doping iron ions into a precursor solution for preparing cobalt hexacyanocobaltate(III) (Co-Co PBAs). The porous FexCo3-xO4 nanocage can be prepared by calcining the prepared doped FeyCo1-y-Co PBAs, wherein x is equal to 0-1 and the size of the nanocage is 50-300nm. The difference between the FexCo3-xO4 nanocage and the same kind of materials previously reported in the literature lies in that the cobalt content of the doped transitionmetal-oxide nanocage prepared by the method is adjustable and the morphology and size of the nanocage are adjustable. The nanocage can be used as a catalyst of a Fenton reaction and a Fenton-like reaction. By activating PMS, PS or H2O2, active free radical species can be produced for degrading organic pollutants in wastewater.

The invention discloses a polylysine oligomer modified recombined apoferritin nanometer cage and a preparation thereof. The nanometer cage is a recombined apoferritin cage with the surface modified by polylysine oligomer that is acquired in the manner of self-assembling protein subunits into hollow spherical protein and utilizing a genetic recombination expression technique to modify different amount of lysine at the N terminal of the protein subunits. The protein nanometer cage can realize the depolymerization and recombination of the protein subunits by changing the solution pH so as to realize the drug loading; the biocompatibility and the in vivo stability are high; the nanometer cage can specifically recognize the high-expression transferrin receptor on the tumor cell surface, so as to realize the active targeting; after the recombined apoferritin nanometer cage is introduced into the cells under the endocytosis effect, the lysosome escape is realized through the proton sponge effect under the lysosome acid environment under the effect of polylysine residue, so that the drug in the protein cage can be protected from being degraded in the lysosome; the recombined apoferritin nanometer cage is expected to have an excellent application prospect at the aspects of gene drug delivery, drug organelle targeting delivery, and the like.

The invention designs a preparation method of a cobalt-nitrogen co-doped carbon nanocage, which specifically comprises the following steps: by using a ZIF-8 structure as an inner core, coating a ZIF-67 shell on the outer layer of the ZIF-8 structure by a seed-mediated growth method; in the process, after Co < 2 + > and 2-methylimidazole are added, taking ZIF-8 spontaneously as an inner core to wrap ZIF-67 to obtain a ZIF-8@ZIF-67 bimetallic core-shell structure; further, in a flowing nitrogen atmosphere at 800 DEG C, carbonizing the organic ligand to form a porous N-doped material, and preliminarily obtaining a cobalt-nitrogen co-doped carbon nanocage material; and performing HCl etching to finally obtain the cobalt-nitrogen co-doped carbon nanocage material. The cobalt-nitrogen co-doped carbon nanocage nanoparticles prepared by the method are good in morphology, uniform in particle, simple in synthesis method step and high in repeatability, have higher activity through morphology and size adjustment, and have a wide application prospect in the fields of electrochemistry, catalysis, biomedicine, virus resistance and the like.

The invention discloses a preparation method of magnetic carbon nanocages, and belongs to the technical field of nano material production. Fe<3>O<4> nanoballs are used as templates and magnetic inner cores, the inner surfaces of the nanoballs are coated with SiO<2> and resorcinol-formaldehyde resin, and the magnetic carbon nanocages are formed after high-temperature calcination and etching. The structure of the product is characterized in that the inner cores are magnetic, and migration of the carbon nanocages can be achieved under an external magnetic field; the outer walls are mesoporous carbon walls, and ions and small molecules can enter the interiors through the outer walls; large gaps exist between the inner cores and the outer walls, and the gaps can load pharmaceutical molecules and catalysts and can also be used as organic reaction sites. Therefore, the magnetic carbon nanocages of an egg yolk-eggshell structure are a catalyst carrier, a sustained-release drug carrier, a micro-nano reactor and a lithium-ion battery electrode material with broad application prospects.

The invention provides a biosensor for detecting Hg<2+> and a preparation method thereof, which can be used in the fields of environmental monitoring, medical treatment and public health, food safety, life sciences and the like. According to the biosensor, a gold nanocage is combined with a Hg<2+> recognition probe by utilizing a surface positive charge modification technique and electrostatic interaction, so that a nanocarrier with a molecular biological door is constructed, and a single-stranded DNA molecular door which is rich in T basic groups is opened through specific molecular recognition reaction, so that fluorescent molecules are released, and the detection of fluorescent signals is realized. Therefore, by utilizing the biosensor, the high-sensitivity and high-selectivity detection of Hg<2+> can be realized. Additionally, on the basis of a controlled release technique of guest molecules in the nanocarrier of the biosensor, a new way and method is provided for the delivery of tumor targeted drugs and the clinical diagnosis and treatment of serious diseases.

Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.

What is presented is an innovative design of a chemical reactor for catalytic breakdown of halogenated hydrocarbons, the body of which acts as a specialized electromagnet capable of immobilizing and retaining very large quantities of non-magnetized ferro-dia- or para-magnetic material-containing nano, micro-, or milli-particles within it's reactor volume despite high-velocity flow of any liquid through said reactor. This is accomplished without any pre-treatment to the nanoparticles, without the use of adhesive of any sort, electroplating, electrolytic action, nano-structured nanocages, membrane or other impregnation, or any other mechanical or chemical means.

The invention relates to a preparation method of a human measles immune sensor based on graphdiyne and a silver nanoparticle cage, and belongs to the technical field of novel biological sensing and detection. Graphdiyne is of a carbon-carbon triple-bond linear structure with an sp hybridized state and has the advantages of zero cis-trans-isomer, high conjugation and the like; due to excellent electrical, optical and photoelectric properties, graphdiyne is a key material of a novel electronic and photoelectric device of the next generation. Meanwhile, by the use of good pi-pi conjugation effect, a high-flux load of signal molecules is realized, and a signal error caused by instable combination is reduced. Therefore, the human measles immune sensor based on graphdiyne and the silver nanoparticle cage can sensitively detect measles viruses.

The invention provides a temperature-sensing gold nano cage and a preparation method and an application thereof, a drug-loaded temperature-sensing gold nano cage and a preparation method thereof, which belong to the technical field of a drug carrier. The gold nano cage is in a hollow porous structure, an external surface of the gold nano cage is coated with a temperature-sensing liposome, the problem that a single photo-thermal treatment in the prior art cannot kill all the tumor cells, single medicine chemotherapy does not have targeting performance, and side effect is large, by taking the gold nano cage as a drug carrier and an optothermal agent, the medicine can realize high-efficiency targeting transmission in body and photo-thermal treatment on tumors; the external surface of the goldnano cage is coated with the temperature-sensing liposome, the photo-thermal conversion performance of the gold nano cage is used, temperature of the temperature-sensing liposome is increased to phase-transition temperature, and the medicine can be rapidly released, so that the photo-thermal-medicine combined high-efficiency controllable malignant tumor treatment effect can be achieved.

An electrode catalyst consisting of the thin-wall carbon nanocages as carrier and Pt nanoparticles and with high electric catalytic activity is prepared through, adding the Fe-C mixture as precursor into a long ceramic test tube, heating in as electric resistance furnace, reacting to generate thin-wall carbon nanocages, cooling, dispersing them in the diluted nitric acid, refluxing, filtering, baking, heating in air for oxidizing, adding alcohol, ultrasonic dispersing, heating by oil bath, pumping chloro-platinic acid to it by peristaltic pump, reacting, and filtering.

The invention discloses nano-drug carrier particles capable of controlling drug release. The particles have a core-shell structure, wherein a gold nano-cage (1) which is provided with a mesoporous surface and has a hollow structure is arranged in the innermost layer; a polymer PAH layer (2) carrying positive electricity is used for modifying the surface of the gold nano-cage (1); a pH sensitive lipid layer (3) is arranged on the outer surface coating layer of the polymer PAH layer (2). Under pH and light external excitation trigger, gating is switched from a 'closed' state to an 'open' state, and drug molecules are released. The carrier particles can be used for effectively improving the efficiency of cancer chemotherapy.

The invention discloses application of gold nanocages in DAN injury resistance. Experiments prove that the gold nanocages help relieve cellular DNA injury induced by active oxygen, chemotherapeutic drugs (such as hydroxyurea) and physical irradiations (such as ultraviolet and X-ray); in manifesting, the gold nanocages promote proliferative activity of cells with DNA injury and inhibit apoptosis ofcells caused by DNA injury; the gold nanocages can evidently inhibit fibroblast apoptosis caused by ultraviolet irradiation and are applicable to the preparation of anti-aging or anti-radiation cosmetics; the gold nanocages as drug carriers feature optothermal release, never stay in the body for long time, and have good biosafety; X-ray irradiation for a mouse model verifies that the gold nanocages can promote reconstruction of the bone marrow blood-producing system, maintain the steady state of the immune system and extend the survival rate, and are applicable to the preparation of drugs toprevent and treat radiation-caused marrow injury or drugs to treat aplastic anemia.

The invention discloses a sodium alginate / dissimilar metal MOFs hollow nanocage hybrid membrane. The hybrid membrane comprises sodium alginate and dissimilar metal MOFs hollow nanocages according to amass ratio 100:2-10; wherein, dissimilar metal MOFs hollow nanocages are prepared by ferric acetylacetone, zinc nitrate hexahydrate and terephthalic acid according to a mass ratio 75:58:12. The preparation comprise the following steps: firstly dissimilar metal MOFs hollow nanocages are obtained; then, the dissimilar metal MOFs hollow nanocages are dispersed in deionized water, the material and sodium alginate are blended in order to prepare a casting solution, spin coating of the casting solution is carried out for a polyacrylonitrile basal membrane, drying, crosslinking and redrying are carried out in order to prepare the hybrid membrane. The product has the advantages of simple preparation process, good controllability, easily available raw materials, general methods, and the like. Thehybrid membrane is used for pervaporation of an ethanol-aqueous solution system, and has high permeation flux and high selectivity for water molecules, and at the same time, the hybrid membrane has good operation stability at a high temperature.

The invention discloses an eggshell-yolk structured cobaltosic oxide-nitrogen doped carbon / carbon nanocage composite material and a preparation method and application thereof. The preparation method comprises the following steps: 1) preparing a carbon nanocage; 2) growing a metal organic framework ZIF-67 crystal in the carbon nanocage prepared in the step 1) in a confinement manner to obtain a ZIF-67 / carbon nanocage composite material; 3) taking the ZIF-67 / carbon nanocage composite material as a precursor, and performing high-temperature calcination to form a cobaltosic oxide-nitrogen-doped carbon / carbon nanocage porous nano composite material; and 4) taking the cobaltosic oxide-nitrogen-doped carbon / carbon nanocage porous nano composite material as the anode material of the lithium ion battery. The composite material prepared by the invention has the advantages of high capacity, good rate capability, strong cycling stability, simple preparation process and the like.

The invention relates to a preparation method and application of a combined targeted medicine controlled release system with a gold nanometer cage being a carrier. The problem that existing tumor treating medicine is large in side effect and poor in treating effect and the medication problem about photothermal therapy of tumors are effectively solved. The method includes the steps that, Re2O3, Fe(NO3)3, Mn(NO3)2, zinc(NO3)2, citric acid, ethylene glycol and the gold nanoparticle cage are added into HNO3, the pH is adjusted to 6, centrifuging is performed, sediment is redissolved through water, the pH is adjusted to 6, drying is performed, and the gold nanometer cage coated with manganese-zinc ferrite is obtained; thermosensitive materials are heated, medicine and the gold nanometer cage coated with the manganese-zinc ferrite are added in, centrifuging is performed, and gold nanometer cage-manganese-zinc ferrite compounds carrying the manganese-zinc ferrite and the thermosensitive materials are obtained; OPSS-PEG-SVA is added into a PBS buffering solution with -NH2 group target head molecules, the gold nanometer cage-manganese-zinc ferrite compounds are added in, centrifuging is performed, sediment is washed through the PBS buffering solution, and the combined targeted medicine controlled release system with the gold nanometer cage being the carrier is obtained. By means of the preparation method and the application, the purposes of thermal therapy, chemical (gene) treatment, targeting and controlled release can be achieved at the same time.

The invention provides a near-infrared two-region fluorescent molecule containing benzobithiadiazole and a preparation method of the near-infrared two-region fluorescent molecule, fluorescent nano-particles and a preparation method and application of the fluorescent nano-particles, and relates to the technical field of fluorescent materials. The near-infrared two-region fluorescent molecule provided by the invention has the characteristics of twisted intramolecular charge transfer and aggregation-induced emission, the quantum yield can be improved, the problem of fluorescence quenching is effectively avoided, and the fluorescence stability is good. The invention also provides the fluorescent nano-particles, which comprise a natural protein nanocage and near-infrared fluorescent molecules encapsulated in the cavity of the natural protein nanocage. The natural protein nanocage is used as a template to encapsulate the near-infrared fluorescent molecules, so that the obtained fluorescent nanoparticles are bright in fluorescence, good in fluorescence stability, high in biocompatibility, uniform in size and can be widely applied to preparation of an angiographic agent or a fluorescent tracer agent.

What is presented is an innovative design of a chemical reactor for catalytic breakdown of halogenated hydrocarbons, the body of which acts as a specialized electromagnet capable of immobilizing and retaining very large quantities of non-magnetized ferro- dia- or para-magnetic material-containing nano, micro-, or milli-particles within it's reactor volume despite high-velocity flow of any liquid through said reactor. This is accomplished without any pre-treatment to the nanoparticles, without the use of adhesive of any sort, electroplating, electrolytic action, nano-structured nanocages, membrane or other impregnation, or any other mechanical or chemical means.

The invention discloses a nanocage probe, an application thereof and a nucleic acid detection method, the nanocage probe comprises gold / silver nanocage particles, the surfaces of the particles are modified with hairpin DNA chains, and the hairpin DNA chains are partially complementary with nucleic acid to be detected; gold / silver nano cage particles are used as a nucleic acid recognition reactionsubstrate and a scattering signal indication probe; a hairpin chain is modified on the surface of the probe as a nucleic acid recognition unit; DNA circulation and HCR amplification processes are realized by utilizing DNA base complementary pairing and strand substitution reaction rules, so that G-quadruplex-heme DNA enzyme is formed on the surface of the probe, etching of a silver component in the nanocage is realized by virtue of active oxygen generated by enzymatic catalysis of hydrogen peroxide decomposition, and a light-off response of a dark field signal is caused. The dark field signalchange with the time resolution capability shows the dependence on the concentration of the nucleic acid to be detected, and quantitative analysis is carried out in combination with a statistical analysis method.