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Photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system

A drug-loading system, gold nanocage technology, applied in the field of anti-tumor, new nano drug delivery system, can solve the problem of low drug loading

Active Publication Date: 2017-06-27
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The drug loading of the above technology is low, although it is not indicated in the article, and secondly, its surface-modified hyaluronic acid has no stimulus responsiveness

Method used

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  • Photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system
  • Photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system
  • Photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system

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preparation example Construction

[0072] Its preparation method comprises the following steps:

[0073] 1) Mix gold nanocages with high-temperature ligands, stir and incubate at room temperature, and centrifuge to obtain high-temperature ligand-modified gold nanocages; wherein, the high-temperature ligands are chain-like thermosensitive polymers containing disulfide bonds, and their phase transitions The temperature is 39-50°C; (The high-temperature ligand is used to close the surface pores of the gold nanocage. The high-temperature ligand shrinks at high temperature, which makes the pores of the gold nanocage open, so that the drug in the gold nanocage is released)

[0074] 2) taking the high-temperature ligand-modified gold nanocages obtained in step 1), dispersing them in an ammonium sulfate solution, stirring, centrifuging, dispersing the solid with an aqueous solution of doxorubicin, and standing in the dark to obtain drug-loaded gold nanocages;

[0075] 3) Mix the drug-loaded gold nanocages obtained in s...

Embodiment 1

[0084] For the preparation of silver nanocubes, add 100mL of ethylene glycol (EG) to a clean 250mL round bottom flask, heat in an oil bath at 150°C, and when the temperature in the flask rises to 150°C, add 1.2mL of 3mM sodium hydrosulfide EG Solution, after two minutes, add 10mL of 3.0μmol / mL hydrochloric acid EG solution, 25ml of 20mg / mL PVP solution, wait for two minutes, add 8mL of 282mM silver trifluoroacetate solution, as the reaction time prolongs, the silver nanocube The size of the continuous growth, the preferred reaction time is 45 minutes, the size is about 60nm (see figure A in Figure 3), and finally placed in an ice-water bath to cool down to terminate the reaction

[0085] Acetone and ultrapure water were used to centrifuge and wash the silver cube solution respectively.

Embodiment 2

[0087] Prepare gold nanocages by electrochemical displacement method, disperse the purified silver nanocubes in ultrapure water, add PVP to a final concentration of 1.5mg / mL, and add 1mM by micro-syringe pump after 10 minutes at 100°C. HAuCl 4 , the flow rate is 42mL / h. With HAuCl 4 With continuous addition, the color of the solution changes continuously. Because the gold ions are continuous with the silver atoms, its structure changes from solid to hollow. At the same time, its maximum characteristic absorption peak continuously changes to the long wavelength of the near-infrared region, and the color of the solution turns blue. At the same time, samples are taken regularly to measure its absorption spectrum. The gold nanocage with the maximum absorption spectrum of 800nm ​​is preferred, so the gold nanocage has a good hollow structure and hole characteristics. see-through effect.

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Abstract

The invention discloses a preparation method of a photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system. The preparation method comprises the following steps of: 1) mixing a gold nanocage with a high-temperature ligand, stirring and incubating the mixture and performing centrifugation to obtain a high-temperature ligand-modified gold nanocage, wherein the high-temperature ligand is a chain-like temperature-sensitive polymer containing a disulfide bond, and the phase change temperature of the high-temperature ligand is 39-50 DEG C; 2) dispersing the high-temperature ligand-modified gold nanocage obtained in the step 1) in an ammonium sulfate solution, stirring and centrifugalizing the mixture, dispersing a solid with an aqueous solution of adriamycin, and leaving the mixture to stand in a dark place to obtain a drug-carrying gold nanocage; and 3) mixing the drug-carrying gold nanocage obtained on the step 2) with a low-temperature ligand, and stirring and incubating the mixture and performing centrifugation to obtain the photo-thermal and chemotherapeutic precise synergic antitumor temperature-sensitive gold nanocage hydrogel drug carrying system, wherein the low-temperature ligand is a chain-like temperature-sensitive polymer containing a disulfide bond, and the phase change temperature of the low-temperature ligand is 26-35 DEG C.

Description

technical field [0001] The invention belongs to the field of pharmacy, and relates to a gold nanocage with photothermal conversion effect, which is a novel nano drug delivery system capable of loading chemotherapeutic drugs, and relates to a preparation method and a drug loading method of the gold nanocage, as well as anti-tumor effects. application. Background technique [0002] At present, cancer is still one of the major diseases threatening human life and health, and the incidence of cancer worldwide is on the rise. The treatment of cancer has always been a difficult point in research at home and abroad. The methods of clinical treatment of cancer include surgery, radiotherapy, hyperthermia and drug chemotherapy. Hyperthermia has attracted widespread attention because of its ability to significantly improve the antitumor effect of drugs. By heating the tumor tissue to 39-42°C, hyperthermia exerted multiple drug-sensitizing effects such as cell apoptosis, changes in tra...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K31/704A61K9/06A61K47/32A61K47/04A61K47/02A61P35/00
CPCA61K9/06A61K31/704A61K41/0052A61K47/02A61K47/32A61K2300/00
Inventor 赵彦兵万江山杨祥良耿石楠
Owner HUAZHONG UNIV OF SCI & TECH
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