The invention discloses a PCB-PTH (Printed Circuit Board-Plated Through Hole) line hole conditioning and activation ultrasonic device and method. The device comprises a hole conditioning or activation solution tank, an ultrasonic generator, a wire and an ultrasonic host, wherein the ultrasonic generator is mounted in the activation solution tank, and the ultrasonic generator is connected with the ultrasonic host via the wire. The PCB-PTH line hole conditioning and activation flow comprises the steps of charging, bulking, recovery, double water washing, glue residue removal, recovery, high neutralization water washing, high double water washing, neutralization, double water washing, hole conditioning, hot water washing, double water washing, microetching, double water washing and the like. According to the invention, ultrasonic energy with the capabilities of compression and expansion can effectively penetrate through fine gaps and small holes due to inertial thrust of liquid, and liquidity of solution in the holes is improved; the difference between colloid particle numbers on a liquid level and at the bottom of the tank can be reduced; palladium colloid molecular dispersion is good; the uniformity of activation of points of a plated board in the tank is improved; the ultrasonic energy generates a micro-stirring force and a temperature difference driving potential which contribute to improving Brownian movement of colloids; and adhesion of hole walls is increased.