Application of gold nanocages in DAN injury resistance

Abstract

The invention discloses application of gold nanocages in DAN injury resistance. Experiments prove that the gold nanocages help relieve cellular DNA injury induced by active oxygen, chemotherapeutic drugs (such as hydroxyurea) and physical irradiations (such as ultraviolet and X-ray); in manifesting, the gold nanocages promote proliferative activity of cells with DNA injury and inhibit apoptosis ofcells caused by DNA injury; the gold nanocages can evidently inhibit fibroblast apoptosis caused by ultraviolet irradiation and are applicable to the preparation of anti-aging or anti-radiation cosmetics; the gold nanocages as drug carriers feature optothermal release, never stay in the body for long time, and have good biosafety; X-ray irradiation for a mouse model verifies that the gold nanocages can promote reconstruction of the bone marrow blood-producing system, maintain the steady state of the immune system and extend the survival rate, and are applicable to the preparation of drugs toprevent and treat radiation-caused marrow injury or drugs to treat aplastic anemia.

Description

technical field [0001] The invention belongs to the field of biomedical material technology and biomedicine, and specifically relates to the application of gold nanocage materials in anti-DNA damage, reduction of cell apoptosis and prevention and treatment of bone marrow damage. Background technique [0002] DNA is the most important genetic material in the life activities of the body, and the maintenance of its molecular structure integrity and stability is of great significance to the survival of cells and the normal physiological activities. However, DNA always faces invasion from inside or outside the organism. DNA damage generally occurs in the life activities of cells, which may lead to cell mutation, cancer and even death. DNA damage can be caused by DNA metabolism, chemical drug (reagent) mutagenesis, ionizing radiation and reactive oxygen damage. DNA damage directly affects DNA replication, transcription and protein synthesis, and further affects life activities su...

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Problems solved by technology

[0003] The current approach against DNA damage is mainly the use of inhibitors of pathways related to the DNA damage response, such as polyadenosine diphosphate-ribose polymerase (PARP) inhibitors, ataxia telangiectasia mutations / ataxia telangiectasia Rad3-associated protein (ATM / ATR) kinase inhibitors, DNA-dependent protein kinase (DNA-PKcs) inhibitors, checkpoint kinase (Chk1 / 2) inhibitors, cyclin-dependent kinase (CDK) inhibitors and DNA alpha Methyltransferase (MGMT) inhibitors, etc., or oxygen free radical scavengers (such as W2721), anti-inflammatory drugs (such as glucocorticoids), cytokines (such as G-CSF), however, these inhibitors generally act on protein Kinases may act on all cells through the phenomenon of synergistic lethality, while hormonal drugs have relatively large side effects, lack of specificity, and have certain destructive power on normal tissues and cells, and these drugs cannot quickly and efficiently remove a large amount of DNA damage produced by the body. Free radicals, also have certain drug resistance

[0004] With the rapid development of the field of nanomaterials, various types of nanomaterials have shown good application prospects in the prevention, treatment and targeted drug delivery of tumors, but no nanomaterials can resist various types of DNA damage. and scavenging reactive oxygen species in cells and promoting cell anti-aging

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