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A kind of medical titanium-based material with drug-loaded layered double hydroxide film and its preparation method and application

A technology of hydroxide and layered double, which is applied in metal material coating technology, coating, medical science, etc., can solve the problems of local loading and release of unsuitable drugs, complicated process, etc., achieve large drug loading capacity and simple process , evenly distributed effect

Active Publication Date: 2019-05-14
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional drug-loaded layered double hydroxides can only be prepared in powder form, which is not suitable for local drug delivery (Chemical Reviews 2012,7:4124-4155)
In addition, drug loading usually needs to be carried out under a protective atmosphere, and the process is relatively complicated
At present, there is no report about the direct construction of layered double hydroxide film on the surface of biomedical titanium or titanium alloy for drug loading.

Method used

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  • A kind of medical titanium-based material with drug-loaded layered double hydroxide film and its preparation method and application
  • A kind of medical titanium-based material with drug-loaded layered double hydroxide film and its preparation method and application
  • A kind of medical titanium-based material with drug-loaded layered double hydroxide film and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] In this embodiment, nickel-titanium alloy among titanium alloys is selected as the substrate, and a nickel-titanium alloy disc with a diameter of 12 mm and a thickness of 1 mm is cleaned with alcohol, deionized water and ultrasonic cleaning in sequence, each time for 15 minutes. With (a) 6mM divalent metal M 1 Soluble salt of NiCl 2 (b) 2mM tetravalent metal M 2 Soluble salt of TiCl 4 (c) A mixed solution of 6.5g / L urea (d) 6mM sodium butyrate was used as a hydrothermal medium for hydrothermal treatment. The hydrothermal temperature was 120°C for 24 hours. The filling degree of the hydrothermal kettle is 35%. Rinse with plenty of deionized water after hydrothermal treatment.

[0039] figure 1 It is the low-magnification and high-magnification scanning electron microscope pictures of the surface morphology of the sample obtained through the modification treatment of this embodiment. It can be seen from the figure that the prepared drug-loaded film presents a sheet-...

Embodiment 2

[0041] Soak the sample treated in Example 1 in phosphate buffer solution (PBS, pH=7.4), take out the extract at regular intervals, and measure the concentration of the model drug sodium butyrate in the extract by an ultraviolet spectrophotometer. After soaking for a certain period of time, 125 μM hydrogen peroxide was added to PBS, and the release amount of sodium butyrate was measured immediately, so as to detect the effect of hydrogen peroxide on drug release. Figure 4 The situation of drug release is given, and it can be seen that before hydrogen peroxide is added, the drug release is at a low level, and after hydrogen peroxide is added, the drug release increases sharply. After removing the hydrogen peroxide in the environment, the drug release returned to a lower level. This shows that the concentration of hydrogen peroxide in the environment can be used as a "switch" to initiate drug release, which can effectively regulate the amount of drug release.

Embodiment 3

[0043] Human cholangiocarcinoma cells RBE and normal intrahepatic bile duct epithelial cells HIBEpiC were cultured in vitro to evaluate the effect of the drug-loaded layered double hydroxide film prepared in Example 1 on the viability of cancer cells and normal cells, using untreated nickel-titanium alloy As a control sample (marked as NiTi) using Alamar Blue (AlamarBlue TM , AbD serotec Ltd, UK) kit to detect the proliferation of cells on the material surface. Methods as below:

[0044] 1) Put the sample sterilized with 75% ethanol into a 24-well culture plate, add 1 mL dropwise to each well with a density of 5×10 4 cell / mL cell suspension;

[0045] 2) Put the cell culture plate into 5% CO 2 Incubate at 36.5°C for 18 hours in a cell culture incubator with saturated humidity;

[0046] 3) Aspirate the cell culture medium, wash the surface of the sample with PBS, transfer the sample to a new 24-well plate, and put it in an incubator to continue culturing;

[0047] 4) After ...

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Abstract

The invention relates to a medical titanium-based material with a drug-loading layered double-hydroxide film and a preparation method and application thereof. The drug-loading layered double-hydroxide film comprises biomedical titanium or titanium alloy, a layered double-hydroxide film and an anti-cancer drug, wherein the layered double-hydroxide film grows on the surface of the biomedical titanium or titanium alloy in situ; and the anti-cancer drug is loaded in crystal lattices of layered double-hydroxide. The titanium or titanium alloy is used as a substrate of the medical titanium-based material provided by the invention, and the layered double-hydroxide film is induced to grow in situ and is used for loading the anti-cancer drug. Owing to the layered double-hydroxide which is a unique crystal structure, exchange between electronegative anti-cancer elements or drug molecules and negative ions among plate layers is realized, and therefore, the drug can be loaded.

Description

technical field [0001] The invention relates to a medical titanium-based material and its preparation method and application, in particular, it relates to a biomedical titanium or titanium alloy as a substrate, with (a) divalent metal ions M 1 Soluble salt of (b) trivalent or tetravalent metal ion M 2 The mixed aqueous solution or hydrosol of the soluble salt of (c) urea, (d) drug is the hydrothermal medium, and the thin film material with selective anticancer and antibacterial ability obtained by one-step hydrothermal reaction in situ growth belongs to the surface modification of metal materials. technical field. Background technique [0002] Titanium and its alloys are widely used as implant materials. For its different medical applications, there are different requirements for material properties. Many applications currently require materials with selective anticancer and antibacterial capabilities. For example, for patients with bone cancer, after the affected area i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/30A61L27/06A61L27/50A61L27/54C23C18/12
CPCA61L27/06A61L27/306A61L27/50A61L27/54A61L2300/404A61L2300/416A61L2300/602C23C18/1204C23C18/1241
Inventor 刘宣勇王东辉
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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