Biosensor for detecting Hg<2+> and preparation method thereof

Abstract

The invention provides a biosensor for detecting Hg<2+> and a preparation method thereof, which can be used in the fields of environmental monitoring, medical treatment and public health, food safety, life sciences and the like. According to the biosensor, a gold nanocage is combined with a Hg<2+> recognition probe by utilizing a surface positive charge modification technique and electrostatic interaction, so that a nanocarrier with a molecular biological door is constructed, and a single-stranded DNA molecular door which is rich in T basic groups is opened through specific molecular recognition reaction, so that fluorescent molecules are released, and the detection of fluorescent signals is realized. Therefore, by utilizing the biosensor, the high-sensitivity and high-selectivity detection of Hg<2+> can be realized. Additionally, on the basis of a controlled release technique of guest molecules in the nanocarrier of the biosensor, a new way and method is provided for the delivery of tumor targeted drugs and the clinical diagnosis and treatment of serious diseases.

Description

technical field

[0001] The present invention relates to a Hg 2+ A sensor and a preparation method thereof, in particular to a sensor for detecting Hg that can be applied in living organisms, food, medicine and the environment 2+ biosensor and its preparation method. Background technique

[0002] As we all know, heavy metal pollution has caused increasingly serious impacts on human health and the living environment. Mercury is a heavy metal with severe physiological toxicity. It has high mobility and bioaccumulation. It is widely distributed in various environmental media and food chains. It circulates in water, soil, atmosphere and biosphere through migration and transformation. Hg 2+ Wastewater pollutants are an important part of water pollution. The infinite mercury pollutants discharged into the water cannot be biodegraded, but participate in the food chain cycle, and can be converted by microorganisms into more toxic methylmercury, which is enriched in large quantitie...

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