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Biosensor for detecting Hg<2+> and preparation method thereof

A biosensor and sensor technology, applied in the field of Hg2+ sensor and its preparation, achieves the effects of strong controllability, good stability, and improved detection sensitivity

Active Publication Date: 2017-05-31
云南通标检测有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The technology of using gold nanocages modified with surface positive charges as nanocontainers and using the electrostatic interaction between T base-rich single-stranded DNA and the surface of gold nanocages to construct biosensors has not been reported in the literature.

Method used

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  • Biosensor for detecting Hg&lt;2+&gt; and preparation method thereof
  • Biosensor for detecting Hg&lt;2+&gt; and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of detection Hg that prepares the present invention proposes 2+ A biosensor method comprising the steps of:

[0023] (1) Take 20 μL of mercapto magnetic beads, wash them with PBS buffer solution, and mix them evenly with 400 μL of gold nanocages, shake at room temperature for 10 hours, magnetically separate, wash with PBS buffer solution, remove the supernatant, and pour the obtained magnetic beads-gold nanocages Add 200 μL of polydiallyldimethylammonium chloride solution with a concentration of 11.664 mg / mL to the cage complex, and shake at room temperature for 10 h;

[0024] (2) Magnetically separate the above solution, wash with PBS buffer solution and add 100 μL of 1.0×10 -5 mol / L rhodamine B solution in PBS, shake at room temperature for 10 h;

[0025] (3) Add 10 μL of the above solution to a concentration of 1.0×10 -5 M’s single-stranded DNA solution rich in T bases, shake at 37°C for 12 hours, magnetically separate, remove the supernatant, and detect Hg...

Embodiment 2

[0028] A kind of biosensor that utilizes the present invention to propose is used for Hg 2+ The detection method is as follows:

[0029] (1) Hg 2+ The sample solution is added to the detection Hg proposed by the present invention 2+ In the biosensor, diluted with PBS buffer solution (PH=7.4), shaken at constant temperature at 37°C for 2h, Hg 2+ The specific molecular recognition reaction with T-rich single-stranded DNA, that is, in the T-Hg 2+ Under the coordination of -T, the single-stranded DNA rich in T bases transforms into a hairpin-like double helix structure, which causes the single-stranded DNA rich in T bases to detach from the surface of the gold nanocage, making it possible to block the pores of the cage. The molecular gate is opened, and the guest molecule rhodamine B in the hole is released;

[0030] (2) Magnetically separate the above solution, collect the supernatant, and perform fluorescence detection, detection conditions: the excitation wavelength and emi...

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Abstract

The invention provides a biosensor for detecting Hg<2+> and a preparation method thereof, which can be used in the fields of environmental monitoring, medical treatment and public health, food safety, life sciences and the like. According to the biosensor, a gold nanocage is combined with a Hg<2+> recognition probe by utilizing a surface positive charge modification technique and electrostatic interaction, so that a nanocarrier with a molecular biological door is constructed, and a single-stranded DNA molecular door which is rich in T basic groups is opened through specific molecular recognition reaction, so that fluorescent molecules are released, and the detection of fluorescent signals is realized. Therefore, by utilizing the biosensor, the high-sensitivity and high-selectivity detection of Hg<2+> can be realized. Additionally, on the basis of a controlled release technique of guest molecules in the nanocarrier of the biosensor, a new way and method is provided for the delivery of tumor targeted drugs and the clinical diagnosis and treatment of serious diseases.

Description

technical field [0001] The present invention relates to a Hg 2+ A sensor and a preparation method thereof, in particular to a sensor for detecting Hg that can be applied in living organisms, food, medicine and the environment 2+ biosensor and its preparation method. Background technique [0002] As we all know, heavy metal pollution has caused increasingly serious impacts on human health and the living environment. Mercury is a heavy metal with severe physiological toxicity. It has high mobility and bioaccumulation. It is widely distributed in various environmental media and food chains. It circulates in water, soil, atmosphere and biosphere through migration and transformation. Hg 2+ Wastewater pollutants are an important part of water pollution. The infinite mercury pollutants discharged into the water cannot be biodegraded, but participate in the food chain cycle, and can be converted by microorganisms into more toxic methylmercury, which is enriched in large quantitie...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6486
Inventor 王卫董颖杰赵娜陈晨罗细亮
Owner 云南通标检测有限公司
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