254results about How to "Highly selective detection" patented technology

The invention discloses a preparation method of an unmarked electrochemiluminescent tumor marker immunosensor and application of the immunosensor in detection of tumor markers, and belongs to the field of novel nano functional materials and biosensors. According to the preparation method of the unmarked electrochemiluminescent tumor marker immunosensor, a novel micro-nano material NH4CoPO4 is taken as a substrate so that the luminescence stability of a luminol-hydrogen peroxide luminescent system can be greatly enhanced, and meanwhile, rod-like gold@siver core-shell nanoparticles are taken as a biomimetic catalytic luminescent system, and therefore, an unmarked electrochemiluminescent immunosensor, which is low in cost, high in sensitivity, good in specificity, fast in detection and simple to prepare and is used for detecting tumor makers, can be prepared.

The invention discloses a preparation method of a sandwich type lung cancer tumor marker immune sensor and the application of the sandwich type lung cancer tumor marker immune sensor to lung cancer tumor marker detection, belonging to the fields of novel nanometer functional materials and a biosensor. According to the preparation method, a precious metal nanometer material, namely a gold @ silver core-shell nanorod is taken as biosimulation enzyme, a second antibody is taken as a marker after being incubated, and nanometer materials including a gold nanorod and reducing graphene are taken as electrode modifiers, so that the sandwich type electrochemical immune sensor which is used for detecting the lung cancer tumor marker and is low in cost, high in sensitivity, good in specificity, rapid in detection and simple in preparation can be prepared.

The invention relates to a preparation method of an aptamer photoelectrochemical sensor for high-sensitivity high-selectivity detection of MC-LR. The preparation method comprises preparing titanium dioxide nanotubes on a titanium matrix by an anodization method, carrying out modification with cadmium sulfide nanoparticles, functionally assembling chitosan and glutaraldehyde to the surface of an electrode so that a photoelectric biological interface is built, and bonding a MC-LR aptamer with a specific base sequence to the surface of the base electrode by chemical bonding so that the MC-LR aptamer photoelectrochemical sensor is prepared. Through combination of a supersensitive photoelectric analysis technology and an aptamer technology with a MC-LR specific recognition function, the preparation method realizes high-sensitivity high-selectivity analysis detection of MC-LR, and has the MC-LR detection limit of 5.3*10<-12> mol / L and a linear detection range of 7.2*10<-8> to 8*10<-12> mol / L. Compared with the prior art, the preparation method has the advantages of simple processes, fast response and low cost and can realize high-sensitivity high-selectivity analysis of MC-LR.

The invention relates to a method for preparing magnetic DNA (deoxyribonucleic acid) supramolecular hydrogel and application thereof. The magnetic DNA supramolecular hydrogel comprises magnetic oxidized graphene and two types of DNA monomers [Y-type DNA monomers (Y-DNA) and linker DNA monomers (L-DNA)]. The Y-DNA and the L-DNA are hybridized by sticky ends in a complementary manner to form DNA hydrogel; exposed sticky ends of the DNA hydrogel are adsorbed on the surfaces of the magnetic oxidized graphene under pi-pi stacking effects to form the magnetic DNA supramolecular hydrogel. The method and the application have the advantages that the magnetic DNA supramolecular hydrogel prepared by the aid of the method is low in cost, easy to operate and separate and high in reaction speed and universality; the method can be successfully applied to colorimetry and ultraviolet-visible absorption quantitative detection on medicine carriers and adenosine triphosphate (ATP); as compared with existing detection methods, the method is easy and convenient to implement and low in cost and has a broad application prospect, visual detection results can be obtained, and complicated instruments or equipment can be omitted.

The invention discloses a preparation method and application of an electrochemical malathion biosensor based on a bi-metal in-situ composite two-dimensional nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel flaky magnetic nano light-sensitive material FeMn-N@TiO2 is prepared for the first time; through good bio-compatibility, large specific area and ferromagnetism of the material, a malathion antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects malathion is prepared.

The invention discloses a preparation method of a photoelectrochemical diethylstilbestrol sensor and belongs to the technical field of novel nano functional materials and biosensors. Firstly, a novel two-dimensional nano photoelectric material, namely a copper-doped nano photoelectric material, specifically a two-dimensional nano composite Cu-TiO2 / MoS2 obtained through in-situ compounding of molybdenum disulfide on a copper-doped titanium dioxide nano cube is prepared, and an diethylstilbestrol antibody is loaded and alkaline phosphatase is fixed to the material by the application of good biocompatibility and a large specific surface area of the material. During detection, due to the fact that alkaline phosphatase can catalyze L-ascorbic acid-2-trisodium phosphate AAP to generate L-ascorbic acid AA in situ and then provide an electron donor for photoelectric detection, by the application of the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the photoelectric current intensity is lowered accordingly, and finally the photoelectric sensor for detecting diethylstilbestrol through an unmarked photoelectrochemical method is constructed.

The invention discloses a three-dimensional carrier of a microarray biochip and a preparation method thereof. The three-dimensional carrier of a microarray biochip is prepared by growing a zinc oxide nanorod on the surface of a substrate material and growing a polymer brush on the surface of the zinc oxide nanorod. The preparation method comprises the following steps of: activating the surface of the substrate material, and growing a zinc oxide nanorod on the surface of the substrate material; performing surface modification on the grown zinc oxide nanorod; connecting the modified surface of the zinc oxide nanorod with an initiator; and finally, growing a polymer brush to obtain a three-dimensional carrier of a microarray biochip. The preparation method is simple, does not need special equipment, has low production cost and good repeatability, and can be used for realizing large-scale production; the prepared three-dimensional carrier has low price and high fixing density for probe molecule, can be used for effectively inhibiting non-specific protein adsorption and improving the sensitivity and the specificity, and can be widely applied to the fields of biology, basic medicine, disease diagnosis, drug screening, food safety, environmental monitoring and the like.

The invention discloses a making method of an unmarked electrochemical immunosensor constructed on the basis of a molybdenum disulfide / silver palladium alloy nanocomposite material, and belongs to the technical fields of novel nanometer function materials and food safety analysis. The sensor made in the invention can be used to detect clenbuterol hydrochloride in a practical sample. The molybdenum disulfide / silver palladium alloy nanocomposite material is prepared through a one-pot technology, and then the simple, fast and sensitive unmarked electrochemical immunosensor for detecting clenbuterol hydrochloride in a meat product is made by using the excellent adsorption and electrochemical catalysis performances of the nanocomposite material.

The invention relates to a nucleic-acid aptamer type photoelectric sensor for detecting atrazine and a preparation method of such nucleic-acid aptamer type photoelectric sensor. The preparation method includes firstly, preparing a three-dimensionally ordered macroporous 3DOM TiO2 / FTO electrode; then, performing Au nanoparticle modification through a hydrothermal reduction method so as to obtain an Au NPs / 3DOM TiO2 / FTO composite electrode; finally, combining an atrazine aptamer onto the surface of the Au NPs / 3DOM TiO2 / FTO composite electrode through an Au-S bond self-assembled film method so as to obtain an Aptamer / Au NPs / 3dom TiO2 / FTO sensor electrode. Compared with the prior art, the nucleic-acid aptamer type photoelectric sensor for detecting the atrazine and the preparation method have the advantages that the nucleic-acid aptamer is used as an atrazine recognition element, so that sensor detection selectivity is improved greatly; since a three-dimensionally ordered macroporous Au NPs / 3DOM TiO2 / FTO composite material of a microstructure is used for recognizing the molecular load of nucleic-acid aptamer element, the photoelectric catalysis performance can be enhanced effectively, atrazine detection sensitivity is improved, the limit of detection is as low as ng / L, high selective recognition is achieved, and the nucleic-acid aptamer type photoelectric sensor can be applied to detection and analysis of trace pollutants accordingly.

The invention relates to a surface-enhanced Raman scattering aptamer sensor for detecting polychlorinated biphenyls and application. Gold-silver core-shell nanoparticles are used as a Raman substrate;4-thiosalicylic acid as a Raman signal molecule is modified on the surface of the gold-silver core-shell nanoparticles; a PCB72 aptamer is used as a pollutant identification element for constructingto obtain the sensor. Compared with the prior art, the surface-enhanced Raman scattering aptamer sensor disclosed by the invention has the advantages that by adopting an advanced surface-enhanced Raman technology, the detection sensitivity of the sensor to PCB72 is greatly improved, the linear detection range of the sensor is 1pg / mL-1000pg / mL and the detection limit can reach 0.3pg / mL; meanwhile,by adopting the aptamer, specific recognition of the PCB72 is realized, and the detection selectivity of the sensor to the PCB72 is greatly improved. The sensor disclosed by the invention can be usedfor detection analysis of an actual water sample; compared with other analysis methods, an analysis method disclosed by the invention has the advantages that a sample does not need complex pretreatment; simplicity, quickness and ultrahigh sensitivity are realized.

The invention discloses an application of an electrochemiluminescence biosensor based on cytosine (C)-containing cyclic DNA sequences where in-situ reduced Ag nano-clusters are enriched as signal probes and adopting dual amplification strategy to detection of target thrombin. According to the technical scheme, DNA cleavage enzyme with hairpin DNA recognition capability and catalysis effect is designed, when target thrombin exists, hairpin DNA is opened, and substrate DNA is cleaved under the action of Zn<2+>; a large quantity of cytosine (C)-containing cyclic DNA sequences are aggregated through HCR (hybridization chain reaction), in-situ reduction of AgNO3 is performed on the electrode surface by NaBH4, a large quantity of Ag nano-clusters are formed, and the electrochemiluminescence biosensor with dual amplification effect is prepared. The sensor is subjected to luminescence detection, and a linear relation is formed between light-emitting signals and concentration of a to-be-testedsample. The Ag nano-cluster signal probes and the DNA dual amplification technology are combined for rapid and high-sensitivity detection of thrombin, and the electrochemiluminescence biosensor has great application potential in early clinical analysis and detection.

The invention relates to a reactive rhodamine fluorescent probe for detecting mercury ions, and a preparation method thereof. A structural formula of the reactive rhodamine fluorescent probe RBHA is shown in the description. The preparation method comprises the steps of adding rhodamine B and hydrazine hydrate into a solvent, reacting for 1-2 h under refluxing at a temperature of 80-90 DEG C; carrying out rotary evaporation; adding water and adjusting pH; filtering; and purifying to obtain rhodamine B hydrazide; dissolving the rhodamine B hydrazide and glyoxylic acid in a solvent; reacting overnight with stirring at a room temperature; filtering; and purifying to obtain the reactive rhodamine fluorescent probe. The reactive rhodamine fluorescent probe has high selectivity for the mercury ions, has no obvious interference with other common ions, can work in an environment with a wide pH scope ranging from 5 to 10, and has application prospects in bioluminescent imaging and analysis of environmental water samples.

The invention relates to a preparation method of a hydrogen sulfide gas sensor, in particular to a gas-sensitive sensor established based on a novel two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and environment monitoring. The sensor can be used for detecting the content of hydrogen sulfide gas in an environment. A cerium and magnesium bi-metal co-doped titanium dioxide nanosheet in-situ composite carbon nitride two-dimensional composite nanomaterial CeMn-TiO2 / g-C3N4 is prepared firstly. Through the characteristics that the material has the large specific area, and high mesoporous gas adsorption and multiple characteristics that electron transfer affects sensitivity due to the material surface gas change, the gas-sensitive sensor which is sensitive to and rapidly responds to the hydrogen sulfide gas is established.

The invention discloses a photoelectrochemical sensor for estradiol based on a boron-doped iron cobalt oxide two-dimensional nano composite material as well as a preparation method and application of the photoelectrochemical sensor. The preparation method comprises the following steps: firstly, modifying a two-dimensional nano material g-C3N4 on ITO (Indium Tin Oxide) conductive glass by adopting an electrodeposition method; secondly, carrying out in-situ grown of iron cobalt oxide by adopting a hydrothermal method, and further preparing to obtain a working electrode loaded with the g-C3N4, doped with boron and modified by the iron cobalt oxide Bi@Fe*Co<1->*O3 / g-C3N4; finally, loading an estradiol antibody by using good biocompatibility and large specific surface area of the material. During detection, L-ascorbic acid-2-trisodium phosphate (AAP) can be catalyzed by boron-doped iron cobalt oxide Bi@Fe*Co<1->*O3 to generate L-ascorbic acid AA in situ, further an electron donor is provided for photoelectric detection, and then photocurrent intensity is correspondingly reduced by using the influence of specific combination of the antibody and antigen on electronic transmission capacity; finally, the construction of the photoelectrochemical sensor for the estradiol by adopting a mark-free photoelectrochemical detection method is realized.

The invention discloses a carbon nitride-binary metal boron oxide composite material-based bisphenol A photochemical sensor and preparation and application thereof. The preparation comprises the steps of firstly modifying a two-dimensional nano material g-C3N4 on ITO conductive glass by adopting an electrodeposition method; growing nickel-cobalt hydroxide in situ by adopting a hydrothermal method to prepare a g-C3N4-loaded and boron-doped nickel-cobalt oxide Bi@NixCo<1-x>O3 / g-C3N4-modified working electrode; and finally loading a bisphenol A antibody by adopting good biocompatibility and large specific surface area of the material, thereby completing preparation of the bisphenol A photochemical sensor. When the detection is implemented, L-ascorbic acid-2-trisodium phosphate APP can be catalyzed by the boron-doped nickel-cobalt oxide Bi@NixCo<1-x>O3 to generate the L-ascorbic acid AA in situ, so that an electron donor is provided for photoelectric detection, the photocurrent strength is correspondingly reduced by utilizing the influence on the electronic transmission capability caused by the specificity quantitative combination of the antibody and antigen, and construction of a photoelectric sensor for detecting the bisphenol A by using an unmarked photoelectrochemical technique is finally achieved.

The invention discloses a preparation method and application of blue-fluorescence carbon quantum dots, and belongs to the technical field of carbon quantum dots. Citric acid and hyaluronic acid are dissolved into water to obtain a mixed solution A; the mixed solution A is subjected to a hydrothermal reaction for 4-12 hours to obtain an HA-CDs solution; the HA-CDs solution is dialysed by using a dialysis membrane and then centrifuged, after standing, supernatant liquid is taken to obtain the blue-fluorescence carbon quantum dots. The hyaluronic acid and the citric acid are used as synthesizingraw materials, the novel carbon quantum dots high in fluorescence intensity is synthesized in one step, high-selectivity detection of folic acid can be achieved, and the carbon quantum dots which areeasily synthesized are applied in biological recognition.

The invention discloses a preparation method of an electrogenerated chemiluminescence sensor, and belongs to the technical field of novel nano-functional materials and biosensors. A novel two-dimensional nano-composite material is firstly prepared, manganese doped titanium dioxide nano-blocks are compounded with molybdenum disulfide in situ to form the two-dimensional nano-composite material Mn-TiO2 / MoS2, aflatoxin antibodies are loaded by the aid of excellent biocompatibility and large specific surface area of the material, manganese doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ when the aflatoxin is detected, electrochemical reaction of O2 and K2S2O8 in base solution are performed to generate electrogenerated chemiluminescence signals, electronic transmission capacity is affected by specific quantitative combination of the antibodies and antigen, so that current intensity is reduced, luminescent intensity is reduced, and the electrogenerated chemiluminescence sensor for detecting the aflatoxin by an unmarked electrogenerated chemiluminescence method is finally prepared.

The invention relates to the technical field of molecular biology, and in particular relates to a self-assembly probe based on configuration change and a label-free detection method for exosomes. Whenexosomes exist, are exosomes surface markers, protein tyrosine kinases-7 (PTK 7) specifically binds to the aptamer, changes the configuration of the recognition probe, releases the trigger chain, andthus binds to G-R DNA undergoes strand displacement reaction, exposing G-rich sequence to form G-quadruplex. NMM is embedded in G-quadruplex, generating strong fluorescence signal, realizing label-free sensitive detection of exosomes. Under the optimized experimental conditions, the linear range is 5*10<5> to 5*10<7> particles / mu L, and the minimum detection limit is 3.4*10<5> particles / mu L. Theprobe is used to prepare a highly sensitive and highly selective detection kit for detecting exosomes in actual blood samples.

The invention relates to a manufacturing method for a hydrogen peroxide non-enzyme sensor based on a silver-graphene nano composite. The manufacturing method comprises the following specific steps of: 1) weighing 5-10mg of graphene oxide, and dissolving the graphene oxide into 5-10 mL of water, thereby obtaining a brown yellow graphene oxide solution; 2) taking a mixed solution containing AgNO3 and KNO3 as an electro-deposition solution; 3) transferring and dispensing 10-15muL of the graphene oxide solution obtained in the step 1) to the surface of a pre-treated glass carbon electrode, and drying in air at the room temperature, thereby obtaining a graphene oxide modified glass carbon electrode; and 4) taking the graphene oxide modified glass carbon electrode as a working electrode, taking a platinum wire electrode as a counter electrode, taking a statured mercurous chloride electrode as a reference electrode, putting the electrodes into the electro-deposition solution prepared in step 2), depositing for 1-3 minutes under constant potential within a range of -0.55V to -0.75V, thereby obtaining a silver-graphene nano composite modified electrode which is used as the hydrogen peroxide non-enzyme sensor. The sensor realizes quick, sensitive and high-selection detection on hydrogen peroxide, and has remarkable advantages in comparison with sensors reported in existing literature.

The invention provides a detection method for glutathione based on a controllable release technology. The method can be used for the fields, such as the early-stage diagnosis and treatment of a serious disease, food, pharmaceuticals, and bioscience. The provided method is used for detecting the glutathione on the basis of a fluorescence sensor of the controllable release technology. The method comprises the following steps: combining a gold nanoparticle cage with a glutathione recognition probe, forming a molecule gate with biological response on the surface of the gold nanoparticle cage through the static assembling function, using the specific molecular recognition reaction generated between the recognition probe and target molecules, causing the recognition probe to be separated from the surface of the gold nanoparticle cage, enabling the molecule gate to be opened, releasing fluorescence molecules in the gold nanoparticle cage, and realizing the strengthening and detection of a fluorescence signal. The method is capable of remarkably improving the detection sensitivity of the glutathione, and realizing the high-sensitive and high-selective detection for the glutathione.

The invention discloses a preparation method of an electrochemical diethylstilbestrol sensor based on a titanium-dioxide-based two-dimensional composite nanomaterial and belongs to the technical field of novel nano functional materials and biosensors. A novel two-dimensional flaky magnetic nano light-sensitive material FeCo-N@TiO2 is prepared firstly; through good bio-compatibility, the large specific area and ferromagnetism of the material, a diethylstilbestrol antibody is loaded, then horse radish peroxidase is fixed through the interlinking effect of glutaraldehyde, in the detection process, due to the fact that horse radish peroxidase can catalyze hydrogen peroxide, electrochemical signals are generated, through the influences of specific quantitative combination of the antibody and an antigen on electron transmission capacity, the current intensity is correspondingly reduced, and finally the electrochemical biosensor which is low in cost, high in sensitivity, good in specificity, rapid in detection and easy to prepare and detects diethylstilbestrol is prepared.

The present invention discloses a preparation method of a label-free electrochemical immunosensor for nonyl phenol detection, and belongs to the technical field of novel nanometer function materials and environment detection. According to the present invention, the molybdenum disulfide / gold-palladium alloy nanometer composite material MoS2 / AuPd is prepared by using the one-pot method, and then the simple, rapid and sensitive label-free electrochemical immunosensor for nonyl phenol detection in real samples is prepared through the excellent adsorption performance and the excellent electrochemical catalytic performance of the molybdenum disulfide / gold-palladium alloy nanometer composite material MoS2 / AuPd.

The invention provides a three-dimensional flower-like nano composite material as well as a preparation method and application thereof, the composite material is NiO / PDDA-RGO, and the material is prepared from PDDA-RGO and synthesized NiO by hydrothermal reaction and calcining via an ultrasonic-assisted method. Reduced graphene oxide in the composite material is used as a NiO carrier, so that theproblem of low NiO conductivity is solved, the adsorption characteristic of an analyte is improved to enhance the electrochemical performance, and the composite material is an excellent electrode modification material. The NiO / PDDA-RGO composite material is modified on the surface of an electrode to construct electrochemical sensing for enzyme-free nitrite detection. According to the method, thetechnical problems of long recognition time, complexity, high cost, low sensitivity, unobvious recognition effect and the like in the prior art are solved, high-sensitivity and high-selectivity detection for nitrite in a complex environment can be realized, and the composite material has a very good application prospect in the analysis fields of environments, foods and the like.

The invention discloses a preparation method of an electrochemical biosensor. According to the method, a novel two-dimensional manometer electrode material-doped titanium dioxide / molybdenum disulfide composite material Cu-TiO2 / MoS2 is firstly prepared, and the material is a copper-doped titanium dioxide manometer square in-situ composite molybdenum disulfide two-dimensional manometer composite material; good biocompatibility and large specific surface areas of the material are utilized; a nonyl phenol antibody is loaded; during detection, copper-doped titanium dioxide can catalyze hydrogen peroxide to generate O2 in situ; an electrochemical signal is generated; the influence of the specificity quantitative combination of the antibody and an antigen on the electronic transmission capability is utilized, so that the current intensity is correspondingly reduced; the building of a biosensor for detecting nonyl phenol by an unmarked electrochemical method is finally realized.

The invention provides a biosensor for detecting Hg<2+> and a preparation method thereof, which can be used in the fields of environmental monitoring, medical treatment and public health, food safety, life sciences and the like. According to the biosensor, a gold nanocage is combined with a Hg<2+> recognition probe by utilizing a surface positive charge modification technique and electrostatic interaction, so that a nanocarrier with a molecular biological door is constructed, and a single-stranded DNA molecular door which is rich in T basic groups is opened through specific molecular recognition reaction, so that fluorescent molecules are released, and the detection of fluorescent signals is realized. Therefore, by utilizing the biosensor, the high-sensitivity and high-selectivity detection of Hg<2+> can be realized. Additionally, on the basis of a controlled release technique of guest molecules in the nanocarrier of the biosensor, a new way and method is provided for the delivery of tumor targeted drugs and the clinical diagnosis and treatment of serious diseases.

The invention relates to a ratio fluorescent fluorine ion probe, and a preparation method and applications thereof in the fluorine ion detection, cell marking, and imaging fields. The probe is mainly composed of two parts. One part is a fluorescence energy donor. The other part is an ionic iridium complex containing an isobutyl diphenyl siloxy group and is taken as an energy acceptor. Two parts are connected through a covalent bond. In the ligand, after the silicon-oxygen bond reacts with a fluorine ion, the silicon-oxygen bond is broken, the excited state and photophysical property of the iridium complex are changed, thus the efficiency of energy transfer between the energy donor and the energy acceptor is influenced, and the ratio fluorescent detection of fluorine ions is realized. The complex can also be applied to biological ratio imaging. The provided compound can be used as a fluorine ion phosphorescence sensor to detect the fluorine ions in water high sensitively and high selectively. The ratio detection and colorimetric detection can be realized, the interference of fluorescence in the background is eliminated, and the detection accuracy is improved. The n represents an integer from 1 to 10.

The invention discloses a preparation method of electrochemical sensor for sulfonamide molecule, and belongs to the technical field of new nano functional materials and biosensor. The method firstly prepares a nickel hydroxide nanosheet array on a disposable throwable electrode, and uses the large specific surface area and a high activity hydroxyl functional group and an amino functional group ofpolydopamine to sequentially prepare a polydopamine film containing an electron mediator and a molecularly imprinted polymer taking a sulfonamide molecule as a template molecule directly on the nickelhydroxide nanosheet array by adopting an in-situ growth method, after eluting the template molecule, the position of the original template molecule becomes a hole, that is, the molecularly imprintedpolymer eluting the template molecule, thus, an electrochemical sensor for the sulfonamide molecule is prepared.

The invention discloses a gold / palladium alloy particle modified tin oxide composite material and a preparation method and application thereof. Tin oxide particles of hollow structure are prepared ina solvothermal method, gold / palladium alloy particle modified tin oxide composite particles in ultra small size are prepared in an in-situ reduction method, and the gold / palladium alloy particle modified tin oxide composite material is obtained via a gentle return process. The material is highly gas sensitive, can serve as a sensing layer of a resistive type metal oxide gas sensor, and can detectdimethyl disulfide gas with a superhigh selectivity; and expensive detection apparatuses are not needed, the detection cost is low, and operation is simple, rapid and efficient.

The gas detecting sensor includes substrate, one layer of diamond film / nano diamond film on one side of the substrate, two metal electrodes connected to the diamond film / nano diamond film and leads; heating resistor on the other side with measuring leads connected to ends. The making process includes making heating resistor, pressure welding measuring leads to ends of the heating resistor; hot wire chemical vapor deposition to prepare diamond, forming nano diamond film via grinding or bias nucleating on the substrate via high temperature reaction of methane or hydrogen; and leading out the metal electrodes. The sensor has high selectivity, high sensitivity, fast response, excellent restore homogeneity, high temperature resistance, high radiation resistance and simple production process.

The invention discloses a organic small molecule containing a boron-nitrogen coordination bond, and a preparation method and sensing application thereof to a fluorine ion, and belongs to the technicalfield of preparation of fluorescent compounds and preparation of fluorescent probes. According to the invention, 2-(3-bromobenzothiophen-2-yl)benzothiazole reacts with n-butyl lithium to form a lithium reagent, and then the lithium reagent reacts with triphenylboron to prepare the small molecule containing the boron-nitrogen coordination bond. Experiments verify that the small molecule containingthe boron-nitrogen (BN) coordination bond prepared by the invention can successfully realize selective recognition of the fluorine ion. The preparation method of the fluorescent compound containing the boron-nitrogen (BN) coordination bond of the invention is simple and convenient, the reaction condition is mild, and a prepared fluorescent probe has the characteristics of photochemical stabilityand good selectivity.