47results about How to "Low detection environment requirements" patented technology

The invention provides a multifunctional rotation device used for carrying out machine vision shooting. The device comprises a supporting foundation, a motor I, a motor mounting seat, a motor shaft coupling, a rotating shaft, a motor II, a camera supporting rack, a tray shaft coupling, a bearing tray, a camera fixing rack and a camera, wherein the motor I is fixed through the supporting foundation, the motor I is connected with the rotating shaft through the motor shaft coupling to drive the bearing tray to rotate, the bearing tray is connected with the rotating shaft through the tray shaft coupling, the motor I and the motor II are fixedly connected through the motor mounting seat, the motor II is connected with the camera supporting rack, and the camera is connected with the supporting rack through the fixing rack. When the camera is required to rotate 360 DEG to shoot a fixed to-be-shot object, the to-be-shot object is arranged on the bearing tray, the camera supporting rack is driven by the motor II to rotate, and the camera shoots after rotating a certain angle; when the camera is required not to move, the to-be-shot object rotates 360 DEG for shooting, the to-be-shot object on the bearing tray is driven by the motor I through an output rotating shaft to rotate, and the camera shots whenever the to-be-shot object rotates a certain angle. The device is advantaged in that through machine vision multi-angle rotating shooting and scanning, object surface quality detection and three-dimensional surface contour reconstruction are realized.

The invention discloses a preparation method of a sandwich type lung cancer tumor marker immune sensor and the application of the sandwich type lung cancer tumor marker immune sensor to lung cancer tumor marker detection, belonging to the fields of novel nanometer functional materials and a biosensor. According to the preparation method, a precious metal nanometer material, namely a gold @ silver core-shell nanorod is taken as biosimulation enzyme, a second antibody is taken as a marker after being incubated, and nanometer materials including a gold nanorod and reducing graphene are taken as electrode modifiers, so that the sandwich type electrochemical immune sensor which is used for detecting the lung cancer tumor marker and is low in cost, high in sensitivity, good in specificity, rapid in detection and simple in preparation can be prepared.

The invention discloses a method for detecting a load swinging angle of a crane. A hoisting hook or a load is connected to a mobile trolley of a crane body through a hoisting rope; more than two wireless network fixing nodes are arranged on the crane body; a wireless network swinging node is arranged on the hoisting hook or the load and respectively communicates with all fixed nodes; the hoisting hook or the load can be spatially positioned on the basis of an RSSI (received signal strength indicator) distance measuring technology; finally, a spatial swinging angle of the load is calculated. The wireless network nodes adopted in the method disclosed by the invention have the advantages of low cost and convenience in installation, and the swinging situation of the load of the crane can be effectively detected, so that the crane can be safely and efficiently produced.

The invention relates to a recombinant protein and test strip for detection of antibodies of 2019 novel coronavirus through a double-antigen sandwich method and a preparation method and application ofthe recombinant protein and test strip, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein for detection of the antibodies of the 2019 novel coronavirus through the double-antigen sandwich method is shown in SEQ ID NO. 1. The recombinant protein is a fusion protein of multiple dominant epitopes of the 2019-nCoV, a reagent for detection of theantibodies of the 2019-nCoV through the double-antigen sandwich method can be prepared, storage at room temperature, fast and single detection with high sensitivity, high throughput and low instrument cost can be achieved at any time, the operation is simple, and convenience of clinical use can be improved greatly.

The invention provides a recombinant protein and test paper for detecting a novel coronavirus 2019-nCoV IgA antibody, and a preparation method and application, and belongs to the technical field of virus detection. The amino acid sequence of the recombinant protein is shown in SEQ ID No.1; the test paper comprises a bottom plate as well as a sample absorption pad, a fluorescent microsphere pad, anitrocellulose membrane and a water absorption pad which are in sequential lap joint and adhesion to the bottom plate; a 2019 novel coronavirus specific antigen marked by fluorescent microspheres is sprayed to the fluorescent microsphere pad; a detection zone and a quality control zone are fixed on the nitrocellulose membrane; an anti-human IgA antibody is sprayed to the detection zone; and a sheep anti-chicken IgY antibody is sprayed to the quality control zone. By detecting the IgA antibody in a saliva sample, the test paper provided by the invention is capable of simply, rapidly and accurate detecting novel coronaviruses, and early-stage detection on the novel coronaviruses can be achieved.

The invention belongs to the technical field of detection and relates to a portable arginine detection device and method. An AuNPs / CQDs fluorescent probe is fixed to filter paper, a paper-based nanometer fluorescent sensor is prepared, a laser in the length of 420 nm is adopted as an excitation wavelength standard in a dark room, and fluorescent signals on the paper-based sensor are directly readby an image collecting device; a fluorescence detection application in the image collecting device is adopted for converting chrominance components of fluorescent images into grayscale components on the basis of a grayscale model, then data processing is carried out, fitting of a grayscale-concentration fitting curve is achieved, and arginine is detected quickly, sensitively and conveniently. Therequirement for a demanded detection environment is low, and detection can be completed at the room temperature without other additional conditions. The portable arginine detection method is high in repeatability, does not require operation of correlated professional staff and can better meet the demands of the market and companies.

The invention provides a dot blot hybridization reaction device and application thereof, and an immunoblotting detection method and relates to the technical field of molecular hybridization experimental devices. The dot blot hybridization reaction device comprises a sampling infiltration module and a suction filtration module used for supplying a negative pressure environment for the interior of the sampling infiltration module, wherein the sampling infiltration module comprises a micropore sampling plate provided with a sample through hole, a support layer provided with an infiltration through hole corresponding to the sample through hole, and a suction filtration tank; a first actuation unit is arranged in the micropore sampling plate; a second actuation unit matched with the first actuation unit is arranged in the suction filtration tank; a closed inner chamber is formed by the matched actuation units in the manner of adsorbing and fixing; and a hybridization film layer and the support layer are successively fixed under the micropore sampling plate. The dot blot hybridization reaction device is portable, is simple in operation and can effectively prevent the problem of cross contamination of sample rooms of the dot blot hybridization reaction device.

The invention relates to a micro-fluidic chip, a nucleic acid detection instrument and a nucleic acid detection method, wherein the micro-fluidic chip comprises a chip main body, an extracting unit, aquantitating unit and an amplification unit, wherein the chip main body is provided with a distal end and a proximal end; the chip main body can rotate around a rotating center near the proximal endthrough being driven by a rotating device; the extracting unit, the quantitating unit and the amplification unit are respectively arranged at the chip main body; the quantitating unit communicates with the extracting unit; the amplification unit is positioned between the quantitating unit and the distal end; and the amplification unit communicates with the quantitating unit. The micro-fluidic chip, the nucleic acid detection instrument and the nucleic acid detection method have the advantages that the operation links of a nucleic acid reagent are reduced; the nucleic acid detection time is shortened; and the effective proceeding of the nucleic acid detection process is ensured.

The invention relates to a device for detecting and adjusting gas outlet flow of a lighter, which solves the technical problem the adjusting nut of the lighter is difficult to adjust at present. The device for detecting and adjusting the gas outlet flow of the lighter comprises a ventilation shaft, a clamp fixing seat and a clamp adjustment piece which are sleeved on the ventilation shaft, and two splints symmetrically arranged on two sides of the clamp fixing seat; the clamp fixing seat is fixed on the ventilation shaft; the middle part of each splint is coupled to the lower part of the clamp fixing seat through a positioning pin; an adjustment hole is formed upward and inward on the upper part of each splint; a movable pin passes through the adjustment hole to connect the upper parts of the splints to the clamp adjustment piece; a clamp port is formed on the inner side of the bottom of each splint; a gas guide passage positioned above the clamp port is formed in the center of the ventilation shaft; and a flow detector is connected with the upper end of the gas guide passage. Compared with the conventional method for directly detecting the height of the flame, a method for adjusting the height of flame by detecting and adjusting the gas outlet flow of a gas outlet needle is more accurate and quicker.

The invention discloses an instrument-free nucleic acid spot quick detection reagent kit. A detection card comprises a sample inlet opening, a sample treatment module, a puncturing pipe, a reaction liquid cavity, a reaction liquid flow channel, a main reaction region, n second reaction regions and corresponding n driving assemblies, a temperature control region, a result region, a result driving assembly, a waste liquid region, a housing and the like, wherein n is greater than or equal to 0. Through the adoption of the detection card, extracting and purifying a nucleic acid sample in advance are not needed, so that spot detection from raw samples to the result is realized, the operation is simple, convenient, quick and low in mistake rate, two-step operations operated by a nonprofessional person are only needed, mistakes and errors brought by different operators are substantially reduced, and the risk of aerosol pollution is also avoided; requirements for sample treatment, detection environment and staff quality are not high, the pollution risk is low, and the false positive rate is very low; the detection card is independent of large-scale instruments or expensive instruments, is low in cost, can be directly applied to detection of spot complex multiple various nucleic acid samples, is also suitable for various detection reactions having multiple similar complex, is wide in application range, and good in application prospects.

The invention discloses a method for fault analysis and location of thyristor controlled reactor of static var compensator,which comprises the step of identifying and locating a fault source gradually by analyzing and calculating the waveform recorded during the fault period,analyzing the abnormalities of the bus line voltage to distinguish the internal fault from the external fault, distinguishing the main link fault or other link / equipment faults in the control system by analyzing whether the three-phase current is abnormal at the same time, and calculating the third-harmonic component in the current to distinguish thyristor valve block control failure from reactor failure. The invention analyzes and locates the fault source of the static var compensator rapidly without relying on laboratory detection or fault simulation, and has the advantages of simplified fault analysis, shortened outage time, improved efficiency, reduced cost and wide application range.

The invention relates to the field of biochemical analysis and detection, and in particular relates to a hairpin probe group based on enzyme assisted cascade cycle amplification, a preparation methodand use. According to the hairpin probe group provided by the invention, a biological target to be detected is used as a first-level target to trigger first target cycle amplification, so that enrichment amplification of a secondary target sequence is realized; simultaneously, the secondary target sequence also can trigger second target cycle amplification; fluorescent signal sequences are continuously released; and thus, cascade cycle amplification of the fluorescent signal sequences is realized. By adoption of the hairpin probe group, a lot of fluorescent signal sequences can be triggered and generated only through trace biological targets; the very high fluorescence signal intensity is generated; the detection sensitivity of the biological target is obviously increased; the detection limit is increased; simultaneously, the specificity of the cascade cycle amplification of the probe in combination with the biological target is high; and a detection result is accurate.

The invention relates to a CFRP porosity ultrasonic characterization method based on ultrasonic backscattered signal recurrence quantification analysis, and belongs to the technical field of nondestructive detection. The method comprises the steps that an ultrasonic signal collecting system composed of an ultrasonic flaw detector, a delay block probe and a digital oscilloscope is adopted, an ultrasonic backscattered signal is collected, and phase-space reconstruction is conducted on the ultrasonic backscattered signal. Distance between every two time vectors in a phase space is calculated, a reference threshold value is set, a two-dimensional recursive matrix is obtained, and the two-dimensional recursive matrix is visualized to obtain a Recurrence Plot (RP). An RP graph is subjected to quantitative calculation through RQA quantitative index Recurrence Rate (RR), the correlation between the CFRP porosity P and RR is established finally, RR=aP-1, and characterization of the porosity is achieved. Compared with an ultrasonic attenuation method, the limitation that when a bottom echo does not exist or is weak, the porosity cannot be characterized is overcome through the method, and a good popularization prospect is achieved.

The invention discloses an automobile differential planet bevel gear spherical surface height detection gauge. The detection tool is composed of a tooth die, a flat plate, a pedestal, a vertical rod, a horizontal rod and a meter tool. The pedestal is a 90-degree V-shaped block. One side of the flat plate is fixedly connected on the horizontally-arranged pedestal. The V-shaped surface of the pedestal is vertical with the upper flat surface of the flat plate to form measurement reference. The top surface of the pedestal is connected with a position-adjustable measuring end which is composed of the vertical rod, the horizontal rod and the meter tool. The tooth die is a section of a coaxial step round sleeve which is small in the upper portion and big in the lower portion. The top surface and the bottom surface of the vertically-positioned tooth die are parallel, and the top surface is provided with an inner tooth ring which is matched with a workpiece to be detected. Tooth part positioning reference adopted in the detection tool is consistent with design reference, so that the installation is free of positioning errors, and the detection precision is high. Moreover, as an open-type detection structure is adopted, the workpiece can be assembled and disassembled conveniently, the detection efficiency is high, and the detection tool is especially suitable to be used in production sites.

The invention relates to a TOF camera stray light detection device and a detection method thereof. The TOF camera stray light detection device comprises a fixed position and a reflecting mirror. The fixed position is used for fixing the TOF camera to be detected; and the reflecting mirror faces the fixed position, and the reflecting surface of the mirror face is in an arc shape back to a fixed position bulge. The TOF camera stray light detection device and the detection method thereof can improve the accuracy of TOF camera stray light detection.

The invention relates to a PCR detector. The PCR detector comprises a rotating device, a micro-fluidic chip and a temperature control device. The micro-fluidic chip comprises a chip main body, an extraction unit, a quantification unit and an amplification unit. The chip main body is arranged on the rotating device, and the chip main body is provided with a proximal end and a telecentric end. The extraction unit, the quantification unit and the amplification unit are sequentially communicated and are respectively arranged on the chip main body, and the extraction unit and the amplification unitare respectively arranged at the telecentric end of the chip main body when the chip main body rotates. The temperature control device comprises a heating assembly and a cooling assembly, the heatingassembly is used for heating the extraction unit and the amplification unit, the cooling assembly is used for cooling the extraction unit and the amplification unit, and the heating assembly is attached to the telecentric end when the chip body rotates. The PCR detector sequentially completes the two steps of nucleic acid extraction and amplification through the micro-fluidic chip, so that the operation link is reduced, and the nucleic acid detection time is further shortened. The heating assembly in the temperature control device has the advantages of high heating efficiency and high heat conduction efficiency.

The invention discloses a rice sheath blight disease identification method and system based on ShuffleNetV2-Unet, and the method comprises the following steps: S1, collecting a rice disease data set which comprises images with rice sheath blight disease marks; s2, establishing a ShuffleNetV2-Unet model, wherein the ShuffleNetV2-Unet model is used for identifying the disease marks of the rice sheath blight disease; s3, the ShuffleNetV2-Unet model is trained by means of the rice disease data set in the step S1, and the trained ShuffleNetV2-Unet model is obtained; and S4, the trained ShuffleNetV2-Unet model is utilized to carry out rice sheath blight disease identification on an input picture. According to the method, the rice sheath blight disease is identified through the ShuffleNetV2-Unet model, the disease marks of the rice sheath blight disease can be effectively identified in a complex environment, the detection speed can be kept while high detection precision is kept, meanwhile, the requirement for the detection environment for detecting the rice sheath blight disease is low, and the operation for detecting the rice sheath blight disease is convenient.

The invention provides an inner gear ring cross-rod distance on-line measuring tool and a measuring method thereof, wherein the measuring tool comprises four gear teeth used for detecting a gear, theincluded angle of the four gear teeth is 90 degrees, and each gear tooth is provided with an installation groove distributed along the radial direction; the four mounting grooves are used for mountinga pair of first measuring units and a pair of second measuring units; the first measuring unit and the second measuring unit are fixedly arranged close to one end surface of the detection gear center; each of the first measuring unit and the second measuring unit comprises a measuring rod, a measuring seat, a permanent magnet, a spring and an electromagnet. According to the invention, the electromagnet loses power to release the permanent magnet, the spring pushes the measuring seat to move along the radial direction, after the equivalent rod abuts against the tooth surface of the inner toothgroove, the fixed displacement values acquired by all the displacement sensors are recorded; finally, the average value of the sum of the fixed displacement values is calculated, which is the cross-rod distance of the inner gear ring. Therefore, rapid on-line measurement of the span rod is realized.

The invention provides a contact type micro-displacement detection device which comprises a hollow shell. A measuring rod is transversely arranged in the shell, the left end of the measuring rod penetrates out of the shell, the upper side and the lower side of the measuring rod are sequentially provided with magnetic control elastomers, piezoelectric patches and U-shaped mounting grooves from inside to outside, and the two U-shaped mounting grooves are arranged back to back, and cavities used for containing the first permanent magnet and the second permanent magnet are formed respectively with the upper inner side wall and the lower inner side wall of the shell, the two piezoelectric patches are connected with leads, a directional plate is fixedly arranged on the right side in the shell, the right end of the measuring rod penetrates through the directional plate and is freely suspended, and a cushion block is arranged on the right inner side wall of the shell. Guide rings are arranged on the left side wall of the shell and the directional plate, a left push plate is fixedly connected to the left side of the measuring rod, a right push plate is fixedly connected to the right side of the measuring rod, a left elastic reset mechanism is connected between the left push plate and the left side wall, and a right elastic reset mechanism is connected between the right push plate and the directional plate. Tiny displacement generated by an object can be accurately detected.

The invention belongs to the technical field of nucleic acid testing, and particularly relates to an instrument-free and power-free rapid on-site nucleic acid testing product. The product comprises a nucleic acid testing module and a temperature control module for providing a nucleic acid testing reaction temperature environment, and is characterized in that the temperature control module includes an air-touch self-heating substance and a ventilation structure; and the temperature control module introduces external air through the ventilation structure during testing. The invention provides the instrument-free and power-free rapid on-site nucleic acid testing product which is simple and convenient to operate, does not depend on large or expensive instruments, is power-free and relatively low in cost, so that nucleic acid testing is realized truly anytime and anywhere, and the application scene of nucleic acid testing is greatly extended.

The invention discloses a system and a method for detecting the out-of-band rejection capability of an ultraviolet imager. The detection system comprises a light source, an integrating sphere, an optical power meter and a calculation control system, wherein the integrating sphere is provided with a light inlet and two light outlets, the light inlet is connected with the light source, one light outlet is used for being connected with equipment to be tested, and the other light outlet is connected with the optical power meter; the calculation control system comprises a light source control unit,an optical power meter data acquisition, processing and display unit and a verification processing unit. And the verification processing unit is used for evaluating the out-of-band rejection capability of the to-be-tested equipment by analyzing the photon number of the to-be-tested equipment and the data of the optical power meter and selecting a photon number evaluation standard. The detection system is simple in structure, low in detection environment requirement, low in detection personnel requirement and convenient to widely popularize and apply.

The invention discloses an instrument-free on-site rapid detection kit for nucleic acid. The detection card includes a sample inlet, a sample processing module, a puncture tube, a reaction liquid chamber, a reaction liquid flow path, a main reaction area, n second reaction areas and corresponding n driving components, n≥0, temperature control area, Result areas, result-driven components, waste areas, enclosures, etc. The test card does not require pre-extraction and purification of nucleic acid samples, and realizes on-site detection from the original sample to the result. It is easy to operate, fast, and less prone to errors. It only requires two-step operations by non-professionals, which greatly reduces errors caused by different operators. It also avoids the risk of aerosol pollution. It has low requirements on sample processing, testing environment and personnel quality. The risk of pollution is small and the false positive rate is extremely low. It does not rely on large or expensive instruments and has low cost. It can be used directly It is suitable for nucleic acid detection of various types of complex and diverse samples on site, and is also suitable for a variety of similar steps and complex detection reactions. It has a wide range of applications and a good application prospect.

A method for rapidly determining rice glutenin content based on quantum dots, comprising the following steps: (1) preparation of CdTe quantum dots; (2) preparation of mother liquor for detection reaction; (3) rapid determination of rice glutenin content by quantum dots The establishment of the method; (4) The pretreatment when detecting rice gluten samples. The price of raw materials for preparing CdTe quantum dots in the present invention is relatively low, the preparation of quantum dots is simple, and the consumption amount is small when the quantum dots are used to quickly measure the glutenin content of rice, and the detection cost is low.

The invention belongs to the technical field of detection of train cracks and relates to a train crack detection device. The train crack detection device comprises a fixed barrel which is provided with a rack, a gear meshed with the rack is arranged below the rack and connected with a first motor, and a baffle is arranged in the fixed barrel and divides the fixed barrel into a first chamber and asecond chamber; a second motor is arranged in the second chamber and fixed to the baffle, and an output shaft of the second motor is connected with a rotating disc; a rotating groove is formed in thefixed barrel in the circumferential direction of the fixed barrel, the rotating disc is located in the rotating groove and can be rotated along the rotating groove, a rotating barrel is fixed to the rotating disc, the end of the rotating barrel penetrates through the fixed barrel and then is provided with a fixing port, and a fixed assembly is fixed in the fixing port; an eddy current sensor is arranged in the rotating barrel and comprises a probe, and the probe is fixed in the fixed assembly; a camera is arranged at the end of the rotating barrel. The labor intensity is low during detection of a hollow shaft of a train, the detection efficiency is high, and the requirements for a detection environment are low.

The invention discloses a reagent and a method for detecting microRNA based on click chemical connection and hairpin stacking assembly. The reagent for detecting microRNA comprises a target recognition probe and a hairpin probe, the target microRNA can be accurately separated from a homologous sequence, and the method does not need various enzymes to participate in the detection process; and the sensitivity is high, the target detection lower limit is 8.63 pM, the specificity is good, the serum adding standard recovery rate is 92.45%-108.92%, and a brand new thought is provided for applying the click chemical reaction and the enzyme-free DNA loop to the field of biological detection in the future.

The invention discloses a thermal barrier coating bonding layer thickness measuring method based on impedance coordinate transformation, which comprises the following steps of: firstly, acquiring an included angle between the thickness of a ceramic layer and the thickness change direction of a bonding layer on an impedance coordinate system by using a standard test piece; performing first impedance coordinate transformation on a to-be-tested piece according to the included angle to obtain an impedance coordinate of the to-be-tested piece which is not influenced by the ceramic layer; then, calculating the thickness of the bonding layer by using the established relationship between the thickness of the bonding layer and the impedance phase; further, correcting the included angle according to the relation between the thickness of the bonding layer and the included angle, carrying out secondary impedance coordinate transformation, and finally calculating the corrected thickness of the bonding layer. According to the method, the problem that in current thermal barrier coating thickness measurement, signals of a bonding layer and a ceramic layer are difficult to decouple is solved by applying an impedance coordinate transformation method, the thickness of the bonding layer can be measured, and the detection efficiency is greatly improved.

The invention belongs to the field of nano functional materials, and discloses formaldehyde detection test paper based on nano-composite fibers and a preparation method and application thereof, and the preparation method comprises the following steps: (1) adding hydrophilic vapor-phase silicon dioxide into a solvent, and carrying out ultrasonic oscillation dispersion; (2) dissolving polyacrylonitrile in the mixed solution in the step (1), and performing stirring to obtain a polymer solution of polyacrylonitrile and SiO2; (3) performing electrostatic spinning on the polymer solution obtained in the step (2) to prepare composite fiber paper; and (4) dipping the composite fiber paper in a color developing treatment solution to obtain the formaldehyde detection test paper, wherein the developing treatment liquid contains a compound containing a hydroxylamine functional group and a derivative of the compound. The test paper provided by the invention has the advantages of visual color change, high selectivity, good stability, portability, normal use under low humidity, lower detection limit than the same type of test paper, and color change with a certain rule along with the increase of concentration; production equipment is simple and easy to operate, and used raw materials and other reagents are low in cost.

The invention discloses a 3D printer planar motion precision measuring device. The 3D printer planar motion precision measuring device comprises a base, an X-direction guide rail, a Y-direction guide rail, an X-direction sliding plate, a Y-direction sliding block, a Y-direction grating reading head, a Y-direction grating scale, an X-direction grating scale, a data processing system, and an X-direction grating reading head. The 3D printer planar motion precision measuring device is advantageous in that a structure is simple, use is convenient, and production costs are low; during use, requirements on testing environment are low, and the 3D printer planar motion precision measuring device can be used in a production scene for measurement; during actual measurement, the data processing system is used for real-time acquisition of position numbers from the X-direction grating reading head and the Y-direction grating reading head, and the CPU is used for the real-time processing of the acquired data, and is used to output actual motion coordinate data including X-direction positions and Y-direction positions to related data communication interfaces; during the phase, the CPU is used to form a dynamic motion curve chart by the fitting of the actual motion coordinate data, and is used to display the dynamic motion curve chart visually by a display screen, and then a user can be aware of an actual measurement result conveniently.