104results about How to "Strong repeatability" patented technology

The invention discloses a method for simulating a biaxial test of a braided composite material and determining material performance parameters by using a finite element method. The method comprises the following steps: simulating a biaxial test of a material by using finite element software based on size and space distribution conditions of fibers and matrixes obtained by a polarizing microscope technology and performances of known component materials, and determining the damage mechanism and process of the material in the test process. The simulation result of the finite element software is compared with true data acquired by a biaxial testing machine, and when the simulation result is almost completely matched with the real data, the method is effective and feasible, and the material damage process simulated by the method is quite credible. The method is simple in operation and strong in repeatability and is suitable for various complex load conditions of the braided composite material, the mechanical response of the braided composite material under the complex load conditions can be accurately and comprehensively reflected, and particularly the microscopic damage mechanism and process of the material which cannot be observed in the test can be intuitively reflected.

The invention discloses an assembling and aligning method for a thermal infrared spectrum imaging system based on a plane-array detector. As the surface of a lens plated with a thermal infrared anti-reflection film has stronger reflection on visible light, the invention provides the assembling and aligning method realized by means of auxiliary objects such as a black body, a slit pair, an auxiliary plane mirror, a precision rotary table, an internal focusing telescope, a collimator tube and the like. The method comprises: placing the black body and the slit pair on a focal plane of the collimator tube to simulate radiation at infinity, and observing the definition of an image of the slit pair on a photosensitive surface of the detector to determine relative positions of lenses and elements. The assembling and aligning method can effectively solve the problem that the thermal infrared spectrum imaging system cannot carry out optical assembling and aligning through visual measurement or other common optical instruments, shortens the assembling and aligning period of the system, and has strong repeatability.

The invention discloses a preparation method of organic acid salt catalytic polyarylene sulfide. The preparation method is characterized by comprising the following steps of: adding 1-50 parts of catalyst, 0.1-20 parts of alkali or strong alkali and weak acid salt, 100-500 parts of organic solvent and 130-140 parts of Na2S.xH2O into a reaction kettle, carrying out dehydration reaction for 0.5-2h at 150-200 DEG C under the protection of nitrogen to obtain a dehydrated Na2S solution, when the temperature of the Na2S solution is reduced to 150-190 DEG C, adding 114-287 parts of dihalogenated aromatic compound, reacting for 0.5-6h at 160-240 DEG C, maintaining the temperature for 0.5-6h at 190 DEG C to 280 DEG C, reducing temperature to 80-140 DEG C, filtering reaction liquid, pouring a filtered reaction product into water, separating a polymer crude product, washing the crude product, purifying by using deionized water and acetone after drying, and drying for 1-10h at 100-120 DEG C under vacuum of 0.08-0.095Mpa, thus obtaining the polyarylene sulfide resin.

The invention provides a waveform-variable automatic impact device for a modal test, and belongs to the field of modal tests. The waveform-variable automatic impact device for the modal test mainly comprises a controller, a digital steering engine, a workbench, a crank-connecting rod mechanism formed by a crank disc and a connecting rod, an impact head device, a sensor, a connecting stud, a linear optical axis guide rail, lubricating components of various motion pairs, corresponding supporting and fixing components and the like, wherein the impact head device comprises an impact hammer cap for damping replacement and a hammer body. According to the invention, the instantaneous rotating speed of the intelligent steering engine is controlled through programming, specific impact waveforms such as triangular waves, pulse waves and the like are outputted, circumferential motions are transformed into reciprocating rectilinear motions by adopting a crank-guide rod mechanism, and the tail end of a guide rod is additionally provided with a hammer to output an exciting force. The contact time between the hammer and an excited portion can also be controlled, and the adjustability of an impact force and the impact time are ensured. A problem that manual knock contact is difficult in excitation time control, poor in repeatability and difficult to excite ideal pulse waves is solved.

The invention provides a preparation method with polysaccharide modified activated carbon as a supercapacitor electrode material. Firstly, a polysaccharide thermal reaction is carried out to obtain a carbon ball, then the carbon ball and potassium hydroxide are mixed and soaked such that the potassium hydroxide is fully diffused into a carbon material, and finally the mixture is subjected to high-temperature calcinations such that the carbon ball is activated by the potassium hydroxide to obtain an activated carbon material. The polysaccharide modified activated carbon material prepared by using the method has the advantages of rich level pore structures and a large specific surface area, as a supercapacitor electrode material, the electrode capacity is high to 510F/g, and the method has the advantages of simple process operation, high reproducibility and low cost.

The invention discloses a coating device and a coating method. The coating device comprises a needle head, a clamp, an imaging device, a height measurement device and a control device, wherein paint is sprayed on the needle head, the clamp is positioned under the needle head, and the clamp and the needle head can relatively move in a transverse direction and a longitudinal direction; the imaging device, the height measurement device and the control device are positioned above the clamp; the needle head can vertically move relative to the clamp; the needle head is connected with a flow control device, and the imaging device, the height measurement device and the flow control device are connected with the control device. During coating, the injection quantity of the paint of the needle head and the opening and closing of a valve are controlled through the flow control device. In the injection process, the control device controls the relative positions of the clamp and the needle head in the transverse direction and the longitudinal direction so as to ensure that the needle head carries out point injection according to a point injection track determined in advance, and controls the needle head to vertically move relative to the clamp so as to ensure that the distance between the needle head and the surface of a product to be coated is constant.

The invention discloses a magnetic separable g-C3N4/Ag/Fe3O4 nanometer composite photocatalysis material and a preparation method thereof. The preparation method comprises the following steps: with melamine as a precursor, preparing g-C3N4 by using a pyrolysis method; then preparing g-C3N4/Fe3O4 by using an in-situ precipitation method; and adding a silver salt solution and carrying out reduction by NaBH4 to complete modification of silver nanoparticles so as to prepare the g-C3N4/Ag/Fe3O4 nanometer composite photocatalysis material. The preparation method is simple and easily practicable, has good repeatability, can realize large-scale production and does not need a template and subsequent treatment; and the prepared photocatalysis material has high visible light catalysis efficiency, can realize magnetic separation of degraded pollutants, can be well recycled and shows wide application prospects in degradation of pollutants in a water body.

The invention belongs to the technical field of detection and relates to a portable arginine detection device and method. An AuNPs/CQDs fluorescent probe is fixed to filter paper, a paper-based nanometer fluorescent sensor is prepared, a laser in the length of 420 nm is adopted as an excitation wavelength standard in a dark room, and fluorescent signals on the paper-based sensor are directly readby an image collecting device; a fluorescence detection application in the image collecting device is adopted for converting chrominance components of fluorescent images into grayscale components on the basis of a grayscale model, then data processing is carried out, fitting of a grayscale-concentration fitting curve is achieved, and arginine is detected quickly, sensitively and conveniently. Therequirement for a demanded detection environment is low, and detection can be completed at the room temperature without other additional conditions. The portable arginine detection method is high in repeatability, does not require operation of correlated professional staff and can better meet the demands of the market and companies.

The invention discloses a tunnel lining structure model test device and test method. The test device comprises a pressure cabin, a stress loading mechanism, a lining layer and a monitoring system. Thepressure cabin comprises a front cabin door, a cabin body and a back cabin door; the lining layer may be poured by plain concrete, reinforced concrete or ring anchor prestressed concrete or the like,and in addition, a surrounding rock simulation layer may be arranged between the lining layer and an inner wall of the cabin body; the stress loading mechanism comprises a loading chamber, a pressuretube, a pressure collection valve and a pressure water pump; the monitoring system comprises a monitoring instrument and a data collection instrument; and the test method comprises the manufacture and installation of the lining layer, the installation of the stress loading mechanism and the monitoring system, loading of different internal and external loads, and data collection and processing analysis.

This invention relates to a gene chip for detecting viruses of bovine infectious diseases. The gene chip comprises a solid carrier and an oligonucleotide probe immobilized on the solid carrier. The oligonucleotide probe is DNA fragment selected from the nucleotide sequences of bovine rabies virus, bovine viral diarrhea virus, bovine foot and mouth disease virus, rinderpest virus and bovine bluetongue virus. This invention also relates to a method for preparing the gene chip and utilizing the gene chip for detecting the five viruses. The method comprises: synthesizing the probe, preparing the gene chip, and hybridizing with a treated sample. The gene chip, together with sample treatment solution, hybridization reagent, color-development reagent and specification, constitutes the test kit for detecting viruses of bovine infectious diseases. The gene chip can detect the five viruses simultaneously, and has such advantages as easy operation, high accuracy and good repeatability.

The invention provides a graphene zinc sulfide nanoparticle composite material and preparation method which relates to the electrode material technical field, the graphene zinc sulfide nanoparticle material is a spherical particle of uniform shape and size and structure of the shell with graphene covering zinc sulfide. The particle diameter is 50-100nm. The graphene zinc sulfide nanoparticle composite material and preparation method has the advantages of simple structure and strong repeatability, the zinc sulfide nanoparticle is disperse evenly in the grapheme, the electrical conductivity is improved and the high the specific capacitance and good circulation stability is provided.

The invention relates to a calibrating device and method of a vehicle profile dimension automatic measuring apparatus, and belongs to the vehicle measuring technical field; the calibration method comprises the following steps: allowing the calibrating device to use an industrial control computer to control a mobile dolly to travel, wherein the mobile dolly is configured with a calibrating plate rotating device; using a laser radar of the vehicle profile dimension automatic measuring apparatus to measure the horizontal distance between the radar and the calibrating plate; using a computer calibrating program to verify the distance measured by the laser radar and the mobile dolly travel displacement; and evaluating the vehicle profile dimension automatic measuring apparatus accuracy. The calibrating device comprises the mobile dolly configured with the calibrating plate rotating device, a customization guide rail, a drive unit, a slave unit, a telescopic scale and the industrial control computer; the calibrating device and method can accurately evaluate the measuring precision of the vehicle profile dimension automatic measuring apparatus measuring the vehicle length, width and height, and can evaluate a laser radar mounting position deviation and angle deviation, thus correcting the whole measuring apparatus. The calibrating method and device are high in detection precision, strong in practicality, and have very well practical values and promotion prospects.

The invention provides an insect epidermal cell line and a construction method. The invention successfully overcomes the difficult problem that the epidermal cell tissue has a high differentiation degree, which is difficult to be cultured in vitro. The steps are that the cell culture solution is prepared firstly; then the insect larvae which enter into the molt cycle are selected for the dissection; the dorsal and abdominal epidermis on the body wall of the worm body is taken and rinsed; the dorsal and abdominal epidermis is minced into tiny tissue fragments which are cultured at 25 degree to 29 degree for the adherence to the wall; 1ml to 2ml cell culture solution is added after 1 hour; 1ml to 2ml cell culture solution is added again after 9 days to 11 days; 1:2 to 1:3 dilution subculture is conducted during the passage of generation every 15 days to 20 days; the serum content in culture solution is decreased to 10 percent to 20 percent at the sixth passage and the growth factor content is reduced to half of the original amount; the serum content in culture solution is decreased to 8 percent to 12 percent at the tenth passage and the growth factor content is deprived from the culture solution; the cell line is successfully constructed. The epidermal cell line which is constructed by the method can be passed continuously; the invention provides a large amount of insect epidermal cells for various biological researches.

The invention discloses a specific primer and an identification method for microsatellite markers of sea horses in coastal waters of China. The identification method comprises the following steps: firstly developing a plurality of sea horse microsatellite markers (SSR); then extracting a sea horse sample DNA; then carrying out PCR amplification on the sea horse microsatellite markers and positive control (a COI gene); carrying out agarose gel electrophoresis on a PCR product; and identifying according to strip type of a plurality of product strips. The identification method disclosed by the invention is not limited by morphological characteristics of the sea horse sample. Regardless of size, freshness or death, deformation and incompleteness of the sample, species identification of sea horses in coastal waters of China can be accomplished by a small amount of DNA, so that the identification method is strong in practicability. The identification method is clear in result, intuitive and clear, strong in repeatability and high in reliability, can be used for detecting a plurality of sea horses or types of sea horses at the same time, and is simple and convenient and efficient and convenient to operate, thereby providing a powerful tool for researches on biological resource survey, germplasm protection, ecoethology and the like on the sea horses in coastal waters of China.

The invention discloses a method for preparing thin films in different components and doping ratios through multi-target co-sputtering. Due to a traditional film coating means of a normal temperaturemagnetron sputtering film coating system, with V being a proportion, the manner of multi-target co-sputtering is introduced to prepare V thin films in different doping components and doping ratios; then an oxidizing annealing process is used for achieving preparation of VO2 thin films in different doping components and doping ratios, so that the phase transformation temperature of the VO2 thin films is adjusted, and wide application in the fields of uncooled infrared focal planes, optical switches, intelligent energy-saving glass and the like can be achieved. Doped atoms can be one or a combination of tungsten, molybdenum, titanium, tantalum, fluorine and niobium, and two or more can be selected for the number of target materials for use according to the specific condition. The method forpreparing the doped thin films through multi-target co-sputtering effectively avoids the problem that different doping components and doping ratios of a traditional sputtering method need a pluralityof different target materials, the preparation cost is greatly lowered, meanwhile, the flexibility and diversity of doping are improved, and the characteristic of being matched with a large-scale filmcoating production line is achieved.

The invention discloses a manufacturing method of a porous microneedle array based on metal sintering. The manufacturing method comprises the following steps that firstly, base mixed liquor is prepared and poured into a base mold, and the bottom of the base mold is provided with multiple conical holes used for manufacturing microneedles; secondly, the base mold with the base mixed liquor poured in the first step is aerated and dried, and a microneedle array obtained after drying and molding are conducted is taken out of the base mold; and thirdly, the microneedle array obtained in the second step is sintered, so that the metal porous microneedle array is obtained. By the adoption of the manufacturing method of the porous microneedle array based on metal sintering, the technology is simple, the structure is stable and the metal strength is high.

The invention discloses a noncellular synthetic biology based preparation method of 2-phenylethanol and an application. According to the preparation method and the application, three recombinases including ARO8, ARO10 and ADH1 are utilized to constitute a co-reaction system, and a complicated metabolic process of an Ehrlich pathway requiring multi-step continuous reaction and multi-enzyme participation for synthesis of 2-phenylethanol in microorganisms is simplified to be performed in the same reaction system in vitro, so that the environmental pollution is reduced and the reaction process is shortened. Furthermore, the three recombinases are subjected to carrier-free co-immobilization with glutaraldehyde serving as a cross-linking agent, Combi-CLEAs (Combi-Cross-linked Enzyme Aggregates) are prepared, a target product is synthesized in vitro, accordingly, the enzymes are further recycled on the basis of the beneficial effects, and the production cost is reduced; the preparation method fills up the technical blank of multi-enzyme preparation of 2-phenylethanol in vitro, and important reference is provided for biological preparation of 2-phenylethanol.

The invention discloses a preparation method of a grapheme-nanometer precious metal composite pipe. The preparation method comprises the steps: (1) immersing a metal wire in dilute nitric acid, placing the metal wire into anhydrous ethanol for ultrasound and then drying; (2) placing the dried metal wire into chemical vapor deposition equipment to grow grapheme on the surface of the metal wire, and obtaining a grapheme/metal wire composite structure; (3) depositing a nanometer precious metal particle coating on the grapheme surface of the grapheme/metal wire composite structure; (4) placing the composite structure obtained into etching liquid, removing the metal wire, then cleaning and drying to obtain the grapheme-nanometer precious metal composite pipe. The preparation method disclosed by the invention has the features of easy operation, high repeatability and no environmental pollution. The grapheme-nanometer precious metal composite pipe provided by the invention is high in mobility, mechanical strength, thermal conductivity and corrosion resistance, and is applied to the fields of microelectronics, transparent electrodes, organic photovoltaic devices, energy storage batteries, multifunctional composites, biomedicine, etc.

The invention discloses a HPLC (High Performance Liquid Chromatography) analysis method for sodium 3,3'-dithiodipropane sulfonate and belongs to the technical field of analysis. The HPLC analysis method is characterized in that a high performance liquid chromatograph with an ultraviolet detector is used for determining; octadecyl silane is taken as a filling column, the size of a chromatographic column is 250mm (length)*4.6mm (inner diameter) and the grain size is 5 microns; methyl alcohol and a buffer solution in volume ratio of (0-50):(100-50) is taken as a flowing phase; the detection wavelength is 190-330nm, and the column temperature is at 10-60 DEG C; the speed of the flowing phase is 0.3-1.2mL/min; the feeding size of a sample is 20 micro liter; the buffer solution contains 0.17mol/L acetic acid, 0.142mol/L triethylamine and ultrapure water. The content of sodium 3,3'-dithiodipropane sulfonate in a to-be-detected sample can be quickly and accurately measured according to a standard curve established via a standard solution; the determination result error is small; the repeated operability is strong; the precision is less than 2.8%; the recovery rate is 96.7%-99.3%; the HPLC analysis method can be widely applied to the production process detection and product quality detection for industrial sodium 3,3'-dithiodipropane sulfonate.

The invention discloses an octacarboxyl-calix [4] arene based terbium metal complex and a preparation method thereof, and belongs to the chemical technical field. The complex is represented by the following chemical formula: [(CH3)2NH2][Tb2(HL)(H2O)7].2H2O, wherein HL is a 2,8,14,20-tetra-amyl-4,6,10,12,16,18,22,24-octa-carboxymethoxy-calix [4] arene anion having seven protons removed. The complex is prepared by a solvothermal method. The fluorescence testing study of the complex indicates that the complex has strong and stable green fluorescence, can realize selective highly efficient detection of organic small molecules N,N'-dimethylformamide and is expected as a fluorescent material to be applied in the field of material science.

The invention provides a fusion method of a pleurotus nebrodensis protoplast and a pleurotus eryngii protoplast. The fusion method is characterized in that hyphal cells of pleurotus nebrodensis and pleurotus eryngii are subjected tot enzymatic hydrolysis for preparation of the protoplasts, the pleurotus nebrodensis protoplast is subjected to the heat inactivation to serve as a mark, and the pleurotus eryngii protoplast is subjected to ultraviolet inactivation to serve as a mark, the pleurotus nebrodensis protoplast and the pleurotus eryngii protoplast are fused by adopting a PEG (Polyethylene Glycol) mediated chemical fusion method, and a fused strain is comprehensively tested through antagonistic experiments, bacterial colony phenotypes, hypha shapes and fruiting experiments in combination of RAPD (random amplified polymorphic DNA). The fusion method can be used for effectively fusing the pleurotus nebrodensis protoplast and the pleurotus eryngii protoplast to obtain a new bacterial strain, the pileus of which is not easy to break and the fruiting body of which is individual; and an effective method is provided for breeding a target bacterial strain through the fusion of the protoplasts.

The invention relates to a set of methods suitable for evaluating toxicity changes of pesticide residues in soil. According to different properties of the pesticide residues in the soil, the set of methods comprises an organic solvent extraction method, a deionized water extraction method and a slurry direct exposure infection method respectively; through an earthworm comet assay and a broad bean micronucleus test, soil ecotoxicity is detected on the basis of double indexes; the set of methods suitable for evaluating the toxicity changes of the pesticide residues in the soil is established, thereby providing a technical support for evaluating influence of toxicities of the pesticide residues on the environment and human health. The set of methods is simple and feasible, high in repeatability and wide in applicability and can be applied to pesticides with different polar properties; by the set of methods, the subsequent determination of the ecotoxicity is facilitated; the set of methods has a relatively great practical application value.

The invention relates to a method for preparing a CoFe alloy/graphene oxide/carbon nanotube composite material. The method adopts urea to treat Fe and Co bimetals. The preparation method comprises thefollowing steps of: adding ferric salt, cobalt salt and urea into deionized water, and performing oil-bath mixing and stirring until drying; uniformly grinding the obtained powder and graphene oxidepowder; calcining under the condition of Ar2; heating to 550 DEG C at a speed of 3-10 DEG C/min, keeping the temperature for 2 hours, heating a tubular furnace to 600-900 DEG C at a speed of 3-10 DEGC/min, keeping the temperature for 2 hours, and naturally cooling to room temperature, thereby obtaining the oxygen reduction catalyst with the CoFe alloy/porous graphene oxide/carbon nanotube multi-dimensional composite structure. The preparation method is simple in preparation process and low in cost; the material has good structure uniformity, has the advantages of high crystallinity, excellentelectrochemical performance and the like, is used for preparing the oxygen reduction catalyst of a zinc-air cell cathode; and the defect that a noble metal catalyst is high in cost, complex in preparation process and difficult to industrialize is overcome.

The invention discloses a construction method for a fat body cell line of a chilo suppressalis larva. The construction method for the fat body cell line of the chilo suppressalis larva includes the following steps: (1) a cell primary culture solution and a cell subculture solution are prepared; (2) a chilo suppressalis larva is soaked in an ethanol solution, surface sterilization is carried out, and the larva body is cleaned and dried through absorption; (3) the larva body is placed in a wax dissection tray disinfected with ethanol, and the chilo suppressalis larva is dissected; (4) a fat body tissue block is placed in a cell culture bottle filled with an HBSS cleaning solution, the cell culture bottle with the fat body tissue block stands still, and the process of pipetting the HBSS and adding fresh HBSS is repeated for three times after fat body cells become adherent; (5) the cell primary culture solution is added, the cell culture bottle is placed into a non-illuminated cell culture box for culture through the night, and then the cell subculture solution is added; (6) the cell subculture solution is pipetted and replaced every 7 days till the culture bottle is filled with expanded and reproduced cells. The construction method for the fat body cell line of the chilo suppressalis larva provided by the invention facilitates chilo suppressalis researches under an isolated culture condition, so as to provide help for the research and development of chilo suppressalis prevention and control methods.

The invention relates to a dual metallic copper/cobalt-organic framework material as well as a preparation method and application thereof. The preparation method comprises the following steps: dissolving cobalt nitrate hexahydrate, copper nitrate and terephthalic acid in an organic solvent to obtain a precursor solution; performing a solvothermal reaction on the precursor solution at the temperature of 150 DEG C; cooling, washing and drying at the end of the reaction to obtain the dual metallic copper/cobalt-organic framework material. Cobalt and copper active center ions highly dispersed in the dual metallic copper/cobalt-organic framework material can enhance effective contact between cobalt ions as well as copper and peroxymonosulfate to produce highly-oxidative sulfate radicals, so that refractory organic pollutants in wastewater are removed. The dual metallic copper/cobalt -organic framework material is suitable for treating various organic wastewater, has the advantages of high efficiency, high durability, convenience in operation and environmental friendliness, and provides a wide prospect for the treatment of toxic, harmful and refractory organic wastewater.

The invention discloses a simple synthetic method for a renewable magnetic carbon micro-sphere with effective adsorption property and an application thereof. The simple synthetic method includes steps of wave synthesis of magnetic ferroferric oxide and secondary microwave synthesis of magnetic carbon micro-sphere. The magnetic carbon micro-sphere is used for adsorbing to remove methylene blue and the like; the magnetic carbon micro-sphere is renewable, and the re-adsorption property is repeated effectively. Compared with a general hydrothermal method, the synthesis time is greatly shortened, the regeneration efficiency is high, the repeated regeneration property is strong, the regeneration can repeat 16 times, and the regeneration rate is 90%.

The invention relates to a rare earth complex based on 2-HNCP and pyridine-3-carboxylic acid as mixed ligands, a preparation method, a crystal structure and an application of the rare earth complex. The molecular formula of the rare earth complex is [RE(2-NCP)2(3-pyc)]n. According to the preparation method, trivalent rare-earth salt, 2-HNCP and pyridine-3-carboxylic acid are mixed in the substance ratio being 1:(0.5-1.5):(0.5-1.5), a certain amount of water is added, the mixture is stirred for a period of time, pH of the mixture is regulated to be 5-8 with an NaOH solution, then the mixed solution is transferred to a reactor with a polytetrafluoroethylene liner, subjected to a reaction in a temperature range of 140-180 DEG C for a period of time and then cooled gradually to room temperature, a product is washed with water and aired at room temperature, and obtained bulk crystals are the rare earth complex. The rare earth complex is applied to aspects of catalysis of organic synthesis, selective adsorption and separation of CO2, CH4, H2, N2, O2 and other gas as well as selective adsorption, separation and photocatalytic degradation of organic dyes and phenolic endocrine disruptors. The synthetic method has the characteristics of high repeatability, high yield, stable product performance and the like.

The present invention provides a production method of a hot-pressing-based kerogen-containing shale physical model and the shale model. The production method comprises the following steps: mixed powder is obtained by ball milling of silica power, calcite, illite and kerogen; a binder is added into the mixed powder for uniformly mixing to obtain a mixed material; the mixed material is layer by layer spread in a hot-pressing die, after each layer of the mixed material is spread, pre-compacting treatment is performed, and a mold filled with the mixed material is obtained; and the kerogen-containing shale physical model can be obtained by final room temperature pre-compacting treatment and heating, pressing and heat and pressure preservation treatment. The production method is a physical model production method which is closest to the natural shale diagenesis environment and diagenetic mechanism. Various petrophysical parameters, mechanical characteristics, seismic properties, microstructure and diagenetic conditions of the kerogen-containing shale physical model are closer to that of natural shale.

The invention discloses a method for manufacturing a decorative crease, comprising the following steps of: 1. drawing crease printing lines on interleaving paper; 2. overlaying creases on the interleaving paper according to the crease printing lines; 3. unfolding the interleaving paper, putting a cut piece between the two pieces of interleaving paper to overlay the creases; 4. forming the interleaving paper overlaid with creases and clamped with the cut piece; and 5. removing the interleaving paper, and overlaying creases and positioning the cut piece according to the crease impression on the cut piece. The method has the characteristic of working hour saving, simpleness in operation and is suitable for mass production.

The invention discloses an improved preparation method of a kelp chromosome. The steps are absorbing surplus moisture after kelp gamobiums are centrifuged, keeping the kelp gamobiums to be deposited, and adding colchicines solution for evenly shaking and stewing; conducting hypotension on the kelp gamobiums by using potassium chloride solution, fixing the kelp gamobiums by sing kano stationary liquid after hypotension, conducting enzymolysis and wall removal through mixed enzyme liquid of cellulose R-10 solution, pectinase solution and liquation enzyme R-10 solution, absorbing supernate by using a liquor relief gun after the kelp gamobiums are centrifuged, adding glacial acetic acid for evenly mixing to form cell suspension, absorbing the cell suspension by using a dropper to an upper dropping sheet of a centrifuging and pre-cooling glass slide, burning the bottom face of the glass slide by using an alcohol burner for one time, and naturally drying or stoving, dying the kelp gamobium chromosome adhered to the glass slide by using giemsa staining fluid, and observing and photographing under a microscope. The chromosomes obtained by using the improved preparation method are good in segregation, differentiable in shape and denumerable in chromosome split pahse, and the improved preparation method is simple and rapid in method, strong in repeatability and capable of opening in a general laboratory.