Portable arginine detection device and method

A detection device and arginine technology, applied in the field of portable arginine detection devices, can solve the problems of low processing efficiency, cumbersome design, complicated operation, etc., and achieve the effects of less sample volume, portable design and strong sensitivity

Inactive Publication Date: 2019-01-04
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are absorption and fluorescence spectrum detection devices based on mobile smart terminals, which can realize real-time on-site, high-precision multi-mode rapid sp

Method used

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  • Portable arginine detection device and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1: Preparation of AuNPs / CQDs

[0058] (1) Preparation of CQDs: Pipette 0.8mL sucrose aqueous solution (30%) and 180μL concentrated sulfuric acid into a beaker, add 5mL PEG 200, place in a constant temperature electromagnetic stirrer water bath heating environment, continue stirring at room temperature for 10min, then microwave for 12s , the power of the microwave oven was 900W, and the solution turned from colorless to golden yellow; the obtained CQDs solution was centrifuged at 5000r / min for 15min to remove insoluble matter, and continued dialysis in a dialysis bag (molecular weight cut-off 1000) for 24h. Then the pH of the CQDs solution was adjusted to 7.0 with an appropriate amount of sodium hydroxide solution, and the prepared CQDs were diluted to 100 mL.

[0059] (2) Preparation of AuNPs: Heat 180mL chloroauric acid (0.3mg / mL) solution continuously from room temperature to 99°C, then quickly add 18mL trisodium citrate solution (9mg / mL) and place it in a con...

Embodiment 2

[0061] Example 2: Preparation of AuNPs / CQDs

[0062] (1) Preparation of CQDs: Pipette 1.2mL sucrose aqueous solution (30%) and 220μL concentrated sulfuric acid into a beaker, add 7mL PEG 200, place in a constant temperature electromagnetic stirrer water bath heating environment, continue stirring at room temperature for 15min, then microwave for 18s , the power of the microwave oven was 900W, and the solution turned from colorless to golden yellow; the obtained CQDs solution was centrifuged at 5000r / min for 15min to remove insoluble matter, and continued dialysis in a dialysis bag (molecular weight cut-off 1000) for 24h. Then the pH of the CQDs solution was adjusted to 7.0 with an appropriate amount of sodium hydroxide solution, and the prepared CQDs were diluted to 100 mL.

[0063] (2) Preparation of AuNPs: 220mL chloroauric acid (0.5mg / mL) solution was continuously heated from room temperature to 99°C, and then 22mL trisodium citrate solution (12mg / mL) was quickly added, pla...

Embodiment 3

[0066] Example 3: Preparation of AuNPs / CQDs

[0067] Pipette 1mL sucrose aqueous solution (30%) and 200μL concentrated sulfuric acid into a beaker, add 6mL PEG 200, place in a constant temperature electromagnetic stirrer water bath heating environment, continue stirring at room temperature for 10min, microwave heating for 15s, the power of the microwave oven is 900W, and the solution is prepared by Colorless to golden yellow; centrifuge the obtained CQDs solution at 5000r / min for 15min to remove insoluble matter, and continue dialysis in a dialysis bag (molecular weight cut-off 1000) for 24h. Then the pH of the CQDs solution was adjusted to 7.0 with an appropriate amount of sodium hydroxide solution, and the prepared CQDs were diluted to 100 mL. The prepared CQDs are in a spherical monodisperse state with a particle size of about 3-5nm, and are stored at 4°C for future use.

[0068] Continue heating 200mL of chloroauric acid (0.4mg / mL) solution from room temperature to 99°C, ...

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Abstract

The invention belongs to the technical field of detection and relates to a portable arginine detection device and method. An AuNPs/CQDs fluorescent probe is fixed to filter paper, a paper-based nanometer fluorescent sensor is prepared, a laser in the length of 420 nm is adopted as an excitation wavelength standard in a dark room, and fluorescent signals on the paper-based sensor are directly readby an image collecting device; a fluorescence detection application in the image collecting device is adopted for converting chrominance components of fluorescent images into grayscale components on the basis of a grayscale model, then data processing is carried out, fitting of a grayscale-concentration fitting curve is achieved, and arginine is detected quickly, sensitively and conveniently. Therequirement for a demanded detection environment is low, and detection can be completed at the room temperature without other additional conditions. The portable arginine detection method is high in repeatability, does not require operation of correlated professional staff and can better meet the demands of the market and companies.

Description

technical field [0001] The invention belongs to the technical field of detection, and relates to a portable arginine detection device and a detection method. Background technique [0002] L-arginine (2-amino-5-guanidinovaleric acid) is a semi-essential amino acid for the human body, an important raw material for the body to synthesize protein and creatine, and plays an important role in protein metabolism, cardiovascular function and immune regulation. important role. In the fermentation industry, L-arginine will be degraded into urea and ornithine under the action of L-arginase, part of urea will be assimilated by yeast, and part will be released into the fermentation broth. Excessive arginine in raw materials will lead to the accumulation of urea in the fermentation broth, and urea will spontaneously react with ethanol to form carcinogenic urethane, causing food safety problems and harmful to human health. In addition, the strong hydrophilicity of L-arginine and the weak...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6428G01N21/6452G01N21/6456
Inventor 邹小波蒋彩萍石吉勇史永强胡雪桃徐艺伟石海军
Owner JIANGSU UNIV
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