51results about How to "Less sample volume" patented technology

This invention provides devices, compositions and methods for determining the concentration of one or more metabolites or analytes in a biological sample, including cells, tissues, organs, organisms, and biological fluids. In particular, this invention provides materials, apparatuses, and methods for several non-invasive techniques for the determination of in vivo blood glucose concentration levels based upon the in vivo measurement of one or more biologically active molecules found in skin.

The invention relates to a method for quickly detecting amyloid protein fiber by thzTHz time-domain light spectrum, which is used for detecting misfolded or denatured protein. The method comprises the following steps: 1. measuring the thzTHz time-domain light spectrum of a series of normal protein corresponding to protein to be detected, and building establishing a light spectrum database; 2). measuring the thzTHz time-domain light spectrum of the protein sample to be detected; and 3). according to the light spectrum database, comparing the light spectrum data of normal protein, and analyzing whether the protein sample to be detected forms amyloid protein fiber. The method has simple process, needs small amount of samples, has short detection time and can perform lossless high-sensitivity detection to amyloid protein fiber.

The invention discloses a method for measuring the antioxidant activity of lactic acid bacteria based on a cellular level and belongs to the technical field of microorganisms. According to the method, a full-wave fluorescent scanning type microplate reader with a 96-hole ferment plate based on the cellular level is utilized to replace a conventional spectrophotometer so as to measure the antioxidant capability of a treated sample; a fluorescent probe and a free-radical initiator are utilized to perform batched rapid and accurate measurement on lactic acid bacteria within 1 hour, so that a rapid detection method is provided for researching the antioxidant activity of the lactic acid bacteria at the cellular level; by utilizing the method, the antioxidant activity of the different strains of lactic acid bacteria can be measured within ultrashort time, and the antioxidant level of the lactic acid bacteria can be rapidly and accurately reflected.

The invention belongs to the technical field of wheat breeding, in particular relates to a wheat breeding method for improving content of resistant starch of kernels and strengthening a health care function. The wheat breeding method for conveniently, rapidly, accurately and efficiently improving content of resistant starch of kernels comprises the steps of: after required variation and breeding generated by variety selection and seed treatment are stabilized, screening materials with reduced main viscosity parameters through starch pasting characteristic measurement, further measuring direct-chain starch content and defining change of physical and chemical properties of starch, and finally confirming materials with increased content of the resistant starch through directly measuring the content of the resistant starch. According to the invention, detection procedures are simplified, selection accuracy and working efficiency are improved, and better effect is obtained in practice.

The invention discloses an identification method of ginger in a traditional Chinese medicine compound and in particular relates to a thin-layer identification method of ginger in a traditional Chinese medicine compound. The identification method comprises the following steps: preparing a reference crude herb solution; preparing a solution of test articles; preparing a negative sample solution; carrying out sample application on a same silica gel G thin-layer plate by using a capillary sample applicator; putting the thin-layer plate in a chromatography cylinder, developing the thin-layer plate with a developer and taking out and airing the thin-layer plate; viewing the thin-layer chromatoplate and identifying the obtained results, wherein the test article chromatogram is in a position corresponding to the reference crude herb chromatogram and shows principal spots in the same color while a negative sample does not have the spots. The identification method is simple in process, has good separating effects and extremely strong specificity, has the effect of further improving the accuracy of identification of the component ginger in the traditional Chinese medicine compound and has huge popularization and application potentials in the field of identification of medicines related to ginger.

The invention relates to the technical field of analysis and detection, in particular to a method for simultaneously detecting trimethylamine oxide and phenylacetylglutamine as well as a detection kit and application thereof. According to the detection method provided by the invention, a to-be-detected sample is subjected to sample pretreatment, then detection is performed by adopting a liquid chromatography-tandem mass spectrometry method, the sample pretreatment comprises the following steps: the to-be-detected sample is subjected to precipitation treatment by adopting a precipitant to obtain separated supernate, and the precipitant is an acetonitrile and methanol mixed solution containing 10-100mM ammonium acetate. The method has the advantages of simple sample reservation, simple sample treatment, small sample use amount and fast detection speed, can reduce the problems of two-time sample reservation, time consumption, cost increase and the like due to respective determination, and is easy to popularize and apply; the detection method has high precision and low matrix effect, and can significantly improve the accuracy of trimethylamine oxide and phenylacetyl glutamine detection.

The present invention provides a method for a method for measuring the properties of liquid based on a quartz crystal microbalance sensor, which employs two measurements to obtain two frequency shifts of the QCM sensor induced by two different volume of the sample liquid. The present invention creatively established the relationship between the density and viscosity of sample liquid and the frequency shifts of QCM sensor. With present invention, the density and viscosity of sample liquid can be obtained through two frequency shifts. Comparing to the conventional liquid property measurement. The measuring procedure of present invention are more simple, and the measuring results are more accurate. Moreover, the present invention consumes less volume of sample liquid, and has the features such as online, real time and quantitative.

The invention relates to a capillary isoelectric focusing analysis method for polypeptide polymer. The method includes the steps of firstly, preparing capillary isoelectric focusing sample-loading liquid, wherein the sample-loading liquid is mixed liquid comprising ampholyte, gel liquid, isoelectric point markers and to-be-analyzed polypeptide polymer; secondly, loading the sample-loading liquid to a capillary column, focusing under 15-30 DEG C and 10-40kV for 2-25 minutes, and migrating under 10-40kV for 20-50 minutes; using a capillary isoelectric focusing instrument or a capillary electrophoresis instrument for detection to obtain isoelectric focusing spectrogram; thirdly, using the isoelectric point markers to calculate the isoelectric point of each component of a polypeptide mixture, and inspecting through the isoelectric point value of each component and the peak pattern of each component. The method can fast and accurately judge whether the components of two polypeptide polymer are identical or not and is low in sample use amount, fast, low in cost and high in result accuracy.

The invention discloses an identification method of patchouli in traditional Chinese medicine compounds. The method comprises the following steps: preparing medicinal material solution; preparing sample solution; preparing negative sample solution; using capillary tube sample applicator to spot samples on a same silica gel G thin layer plate; placing the thin layer plate into a chromatographic tank, using developer to develop, taking out, drying in air; checking the chromatoplate to obtain the results to be identified, the sample chromatoplate coincides with the medicine chromatoplate in corresponding positions by indicating fluorescent main spots with the same color. The method has the advantages of easy identification method and process, good separation effects and utmost specialization. The method further elevates the identification correctness of patchouli in traditional Chinese medicine compounds, bears great potential of the promotion and application in the field of medicine identification related with patchouli.

The invention relates to a method for evaluating the quality of coke for a lithium battery negative electrode material, which comprises the following steps: S1, airing a green coke sample, performingcoarsely crushing, and completely performing screening by 4mm to obtain primary coke powder; s2, carrying out heat treatment on the primary coke powder in a nitrogen atmosphere at 700-800 DEG C, and then crushing the primary coke powder until the primary coke powder is completely sieved by a 0.071 mm sieve, so as to obtain secondary coke powder; and S3, carrying out vacuum drying on the secondarycoke powder at 110-140 DEG C, performing heating from 50 DEG C to 900 DEG C at a rate of 2-5 DEG C/min in an air atmosphere, carrying out thermogravimetric detection, and determining the reaction starting temperature of graphitization degree change and the reaction starting temperature of reversible capacity change. Compared with the prior art, the method has the beneficial effects that the quality grade of the green coke for the negative electrode can be rapidly distinguished, the sample consumption is small, and the analysis is rapid and sensitive; and compared with the traditional method, the method has the advantages of simplicity, convenience, time saving and the like in early-stage sample preparation.

The invention provides a quality control method of amygdalin in children lung heat cough and asthma granules. A thin-layer chromatography identification method has the advantages of small sample application amount, good separation effect, clear spots, moderate specific shift value and good reproducibility. Quantitative analysis is carried out on the amygdalin component in the infantile lung-heat cough and asthma granules through content determination, it is verified that the content of the amygdalin in the infantile lung-heat cough and asthma granules can be accurately and efficiently detected through content determination chromatographic conditions, and through combination of a thin-layer chromatography identification method and content, the content of amygdalin in the children lung heat cough and asthma granules can be effectively controlled, and the medication safety of the children lung heat cough and asthma granules is ensured.

The invention discloses a method for distinguishing rhizoma atractylodis in a traditional Chinese medicine compound and particularly relates to a method for thin-layer distinguishing of the rhizoma atractylodis in the traditional Chinese medicine compound. The distinguishing method comprises the following steps that a control medicinal material solution is prepared; a sample solution for test is prepared; a negative sample solution is prepared; sample application is performed on the same silica gel G thin-layer plate by using a capillary tube sample applicator; the thin-layer plate is arranged in a chromatography cylinder, development is performed by using a developing solvent, and the plate is taken out and air-dried; the thin-layer chromatographic plate is inspected, an obtained result is distinguished, main fluorescence spots of the chromatography of the sample solution for test and the control medicinal material are located in the corresponding positions and have same color, and the negative sample is free of main fluorescence spots having the same color. The distinguishing method is simple in process and good in separating effect, has strong specificity, further improves the accuracy of distinguishing the rhizoma atractylodis component in the traditional Chinese medicine compound and has huge popularization and application potential in the drug distinguishing fields relevant to the rhizoma atractylodis.