51results about How to "Less sample volume" patented technology

A disposable electrode strip for testing a fluid sample including a laminated strip with a first and second end, a vent, an open path for receiving a fluid sample of less than one microliter beginning from the first end and connecting to the vent, a working electrode, a reference electrode and a pseudo-working electrode embedded in the laminated strip within the open path and proximate to the first end, a reagent matrix coextensive within the open path and covering the three electrodes, and conductive contacts located at the second end of the laminated strip.

This invention provides devices, compositions and methods for determining the concentration of one or more metabolites or analytes in a biological sample, including cells, tissues, organs, organisms, and biological fluids. In particular, this invention provides materials, apparatuses, and methods for several non-invasive techniques for the determination of in vivo blood glucose concentration levels based upon the in vivo measurement of one or more biologically active molecules found in skin.

The invention relates to an online analysis method for sulfur isotope of hydrogen sulfide gas in natural gas. The method comprises the steps of collecting a natural gas sample; heating oxidation furnace of an elemental analyzer to 1,020 DEG C; heating a chromatographic column box to 50 DEG C; introducing reference gas into a stable isotope ratio mass spectrometer, adjusting center magnetic field of the peak with mass / charge ratio of 66, and storing the magnetic field value; connecting a steel cylinder filled with the natural gas sample with a sampling valve, placing in a ventilating hood, and switching on the ventilating hood; taking the natural gas sample with an airtight sample injector; injecting the natural gas sample into a sample inlet; introducing the natural gas sample into a high-temperature oxidation tube with carrier gas, to be oxidized into carbon dioxide, water and sulfur dioxide gas, carrying the mixed gas with carrier gas through a dewatering tube for water removal; separating the dewatered mixed gas with a gas chromatographic column, sequentially introducing into a stable isotope ratio mass spectrometer through a continuous flow interface, measuring sulfur isotope composition, and generating spectrogram.

The invention relates to a method for quickly detecting amyloid protein fiber by thzTHz time-domain light spectrum, which is used for detecting misfolded or denatured protein. The method comprises the following steps: 1. measuring the thzTHz time-domain light spectrum of a series of normal protein corresponding to protein to be detected, and building establishing a light spectrum database; 2). measuring the thzTHz time-domain light spectrum of the protein sample to be detected; and 3). according to the light spectrum database, comparing the light spectrum data of normal protein, and analyzing whether the protein sample to be detected forms amyloid protein fiber. The method has simple process, needs small amount of samples, has short detection time and can perform lossless high-sensitivity detection to amyloid protein fiber.

The invention relates to a beet syrup adulterated honey identification method, and aims to provide an accurate and fast beet syrup adulterated honey identification method to solve the problem that beet syrup is adulterated in the conventional counterfeited honey, and discloses a beet syrup adulterated honey detection method. The beet syrup adulterated honey identification method comprises three steps of pretreatment of a sample, liquid chromatographic separation and mass spectrum detection, and is a liquid chromatography-mass spectrography detection method taking 3-methoxy-tyramine of which the mass-to-charge ratio is 168.1016 as a characteristic marker of beet syrup.

The invention discloses a method for measuring the antioxidant activity of lactic acid bacteria based on a cellular level and belongs to the technical field of microorganisms. According to the method, a full-wave fluorescent scanning type microplate reader with a 96-hole ferment plate based on the cellular level is utilized to replace a conventional spectrophotometer so as to measure the antioxidant capability of a treated sample; a fluorescent probe and a free-radical initiator are utilized to perform batched rapid and accurate measurement on lactic acid bacteria within 1 hour, so that a rapid detection method is provided for researching the antioxidant activity of the lactic acid bacteria at the cellular level; by utilizing the method, the antioxidant activity of the different strains of lactic acid bacteria can be measured within ultrashort time, and the antioxidant level of the lactic acid bacteria can be rapidly and accurately reflected.

The invention discloses a detection method of graphene oxide test paper for identification of collagen peptide. The method comprises the following steps: (1) substrate preparation: modifying a nano material capable of fluorescence resonance energy transfer to absorbent paper, cutting the absorbent paper into rectangular of 3+ / - 0.3mm*1+ / - 0.1mm, drying naturally at 20-40 DEG C, dropwise adding 0.5-1 [mu]l 20mM PBS buffer on the substrate and drying naturally; (2) probe preparation: modifying a peptide with specific sequence by fluorescent molecules; (3) probe and substrate hybrid: dropwise adding 0.5-1 ul of a probe peptide solution to the substrate for hybridization, incubating for 10-20min and drying naturally; and (4) identification of collagen peptide: premixing an analyte and probe peptide, dropwise adding 0.5-1 [mu]l of the mixed liquid to the substrate, incubating for 20-40 min, and drying naturally; and exciting by using excitation wavelength of fluorescent molecules in step (2) in the dark, and detecting the degree of fluorescence recovery. The test paper has the advantages of low cost, simple operation, convenient detection, good specificity, and small sample amount.

The invention belongs to the technical field of wheat breeding, in particular relates to a wheat breeding method for improving content of resistant starch of kernels and strengthening a health care function. The wheat breeding method for conveniently, rapidly, accurately and efficiently improving content of resistant starch of kernels comprises the steps of: after required variation and breeding generated by variety selection and seed treatment are stabilized, screening materials with reduced main viscosity parameters through starch pasting characteristic measurement, further measuring direct-chain starch content and defining change of physical and chemical properties of starch, and finally confirming materials with increased content of the resistant starch through directly measuring the content of the resistant starch. According to the invention, detection procedures are simplified, selection accuracy and working efficiency are improved, and better effect is obtained in practice.

The invention discloses an identification method of ginger in a traditional Chinese medicine compound and in particular relates to a thin-layer identification method of ginger in a traditional Chinese medicine compound. The identification method comprises the following steps: preparing a reference crude herb solution; preparing a solution of test articles; preparing a negative sample solution; carrying out sample application on a same silica gel G thin-layer plate by using a capillary sample applicator; putting the thin-layer plate in a chromatography cylinder, developing the thin-layer plate with a developer and taking out and airing the thin-layer plate; viewing the thin-layer chromatoplate and identifying the obtained results, wherein the test article chromatogram is in a position corresponding to the reference crude herb chromatogram and shows principal spots in the same color while a negative sample does not have the spots. The identification method is simple in process, has good separating effects and extremely strong specificity, has the effect of further improving the accuracy of identification of the component ginger in the traditional Chinese medicine compound and has huge popularization and application potentials in the field of identification of medicines related to ginger.

The invention relates to a method for extracting nucleic acids with urine as a sample in the biological field, and application thereof, and provides a special kit and method for rapidly extracting the nucleic acids from a large amount of urine. The method comprises the following steps: enriching the nucleic acids in the urine onto a nucleic acid adsorbing column in a suction filtration column manner, washing, eluting and the like for extracting a trace amount of nucleic acids in the urine. The nucleic acids obtained by using the kit provided by the invention comprise free nucleic acids in the urine and nucleic acids in exfoliated cells in the urine; the operation is simple and rapid; the nucleic acids are high in yield and high in purity; the kit and the method can be directly applied to molecular biological detection.

The invention relates to the technical field of analysis and detection, in particular to a method for simultaneously detecting trimethylamine oxide and phenylacetylglutamine as well as a detection kit and application thereof. According to the detection method provided by the invention, a to-be-detected sample is subjected to sample pretreatment, then detection is performed by adopting a liquid chromatography-tandem mass spectrometry method, the sample pretreatment comprises the following steps: the to-be-detected sample is subjected to precipitation treatment by adopting a precipitant to obtain separated supernate, and the precipitant is an acetonitrile and methanol mixed solution containing 10-100mM ammonium acetate. The method has the advantages of simple sample reservation, simple sample treatment, small sample use amount and fast detection speed, can reduce the problems of two-time sample reservation, time consumption, cost increase and the like due to respective determination, and is easy to popularize and apply; the detection method has high precision and low matrix effect, and can significantly improve the accuracy of trimethylamine oxide and phenylacetyl glutamine detection.

The present invention provides a method for a method for measuring the properties of liquid based on a quartz crystal microbalance sensor, which employs two measurements to obtain two frequency shifts of the QCM sensor induced by two different volume of the sample liquid. The present invention creatively established the relationship between the density and viscosity of sample liquid and the frequency shifts of QCM sensor. With present invention, the density and viscosity of sample liquid can be obtained through two frequency shifts. Comparing to the conventional liquid property measurement. The measuring procedure of present invention are more simple, and the measuring results are more accurate. Moreover, the present invention consumes less volume of sample liquid, and has the features such as online, real time and quantitative.

The invention relates to a capillary isoelectric focusing analysis method for polypeptide polymer. The method includes the steps of firstly, preparing capillary isoelectric focusing sample-loading liquid, wherein the sample-loading liquid is mixed liquid comprising ampholyte, gel liquid, isoelectric point markers and to-be-analyzed polypeptide polymer; secondly, loading the sample-loading liquid to a capillary column, focusing under 15-30 DEG C and 10-40kV for 2-25 minutes, and migrating under 10-40kV for 20-50 minutes; using a capillary isoelectric focusing instrument or a capillary electrophoresis instrument for detection to obtain isoelectric focusing spectrogram; thirdly, using the isoelectric point markers to calculate the isoelectric point of each component of a polypeptide mixture, and inspecting through the isoelectric point value of each component and the peak pattern of each component. The method can fast and accurately judge whether the components of two polypeptide polymer are identical or not and is low in sample use amount, fast, low in cost and high in result accuracy.

The invention discloses a quick detection method of a carotenoid content in a marine bivalve organism. The method comprises the following steps: (1) sampling and pretreatment of tissues; (2) tissue slicing and chromatic aberration detection; (3) total carotenoid content (TCC) detection; (4) establishment of a chromatic-aberration-TCC regression curve; and (5) total carotenoid content (TCC) detection of an adductor muscle of a to-be-detected marine bivalve organism. According to the method, the adductor muscle tissue of the marine bivalve organism is sliced and is subjected to chromatic aberration detection, and chromatic aberration is associated with the carotenoid content to establish a chromatic-aberration-TCC equation of linear regression. Therefore, the carotenoid content detection of the organism is detected through a corresponding chromatic aberration parameter. The method is free of extra sample consumption, has good repeatability and practicability, and is quick and efficient.

The invention discloses an analysis method for detecting furans compounds and pyrans compounds in beer, which combines a solid-phase extraction analysis method and a gas chromatogram / mass spectrometry analysis method. The analysis method comprises the following steps of preparation, solid-phase extraction, gas chromatogram / mass spectrometry analysis, detection and the like. The analysis method for detecting volatile furans compounds and pyrans compounds in beer is established and has the advantages of rapidness, small sample usage, accuracy, sensitiveness, high efficiency and the like. Small polystyrene / divinyl benzene columns selected in the solid-phase extraction process have the characteristics of strong adsorption capability, small organic solvent usage, convenience, safety, high efficiency and the like.

The invention relates to a method for determining antioxidant capacity of ocean shellfishes, in particular to a method for rapidly determining total antioxidant capacity of marine bivalves, which replaces a conventional spectrophotometer with a full wave frequency scanning type microplate reader a 96 ferment plate for determining total antioxidant capacity of processed samples, can determine batches of samples rapidly and accurately in a short time and accordingly can detect interspecies resistance study of the marine bivalves rapidly. The method rapidly determining total antioxidant capacity of marine bivalves can determine the total antioxidant capacity of the marine bivalves with different varieties and different textures in batch mode, and determination speed is improved by about 10 times compared with a conventional method.

The invention discloses a TLC identification method for Nazhenning, which belongs to the technical field of quality control of traditional Chinese medicine extracts and preparations. The invention provides the identification of tangerine peel in Naozhenning. The preparation method of the test product is simple, fast, accurate and environmentally friendly. The crude drug contained in it can be quantified, the separation degree is high, the sampling volume is small, the sampling volume is controllable, the sensitivity is high, and the interference is less. The invention provides the identification of Cortex Moutan in Naozhenning, using methanol ultrasonic extraction, water-saturated n-butanol extraction, neutral alumina mixing sample, can obtain the test sample, the sample amount is small, save time, and reduce environmental pollution Less, faster and easier to operate. The method provided by the invention has strong specificity, good reproducibility and durability, and can be used for the identification of the corresponding medicinal flavors in the extracts and preparations of Zhenzhengning.

The invention discloses an identification method of patchouli in traditional Chinese medicine compounds. The method comprises the following steps: preparing medicinal material solution; preparing sample solution; preparing negative sample solution; using capillary tube sample applicator to spot samples on a same silica gel G thin layer plate; placing the thin layer plate into a chromatographic tank, using developer to develop, taking out, drying in air; checking the chromatoplate to obtain the results to be identified, the sample chromatoplate coincides with the medicine chromatoplate in corresponding positions by indicating fluorescent main spots with the same color. The method has the advantages of easy identification method and process, good separation effects and utmost specialization. The method further elevates the identification correctness of patchouli in traditional Chinese medicine compounds, bears great potential of the promotion and application in the field of medicine identification related with patchouli.

Proteases regulate a wide range of normal cellular functions where dysregulated activity is observed in various diseases. Compositions and methods use protease activity multiplexed bead-based immunoassays to profile protease activity. This platform technology integrates protease activity measurements with total protein quantification techniques. It represents a significant improvement over existing detection techniques by allowing for multiplexed, sensitive active protease measurements in complex biological samples. Exemplary multiplexed detections are realized in a single assay using a minute sample amount (e.g., 5 μl) for active recombinant MMP-1, -2, -3, -7, 9, and 12 and those same MMPs in cell culture supernatant, menstrual fluid effluent, and peritoneal aspirates. This multiplexed platform achieves high level of sensitivities equal to or better than existing leading single-plex detection strategies. It also allows for high throughput screening to identify inhibitors of proteases in complex, donor-derived samples.

The invention provides a method and a kit for detecting antiasthmatic drugs in serum. In the method, the antiasthmatic drugs are theophylline, diprophylline and doxofylline. The method comprises the steps that a serum sample is extracted and then detected and analyzed through an HPLC-MS method, and an extracting solution is a mixed solution of methanol containing trichloroacetic acid and acetonitrile. According to the method, the separation degree can be improved, the detection time can be effectively shortened, the analysis and determination efficiency is improved, and the problems of low detection signal and low sensitivity are solved; meanwhile, the method is good in linearity and high in feasibility.

The invention discloses a method for ignition-free closed flash point determination of petroleum products. The method includes: heating a to-be-tested petroleum product to dry out; collecting a firststeam temperature and the liquid temperature of the petroleum product according to preset sampling frequency; collecting steam differential pressure of the petroleum product according to preset collection frequency; preprocessing data of the first steam temperature and the steam differential pressure to obtain a changing curve of normalization integrals of the steam differential pressure and a second steam temperature of the to-be-tested petroleum product; substituting the changing curve of normalization integrals of the steam differential pressure and the second steam temperature of the to-be-tested petroleum product into an established multi-element correction model to obtain closed flash point results of the petroleum product in a distillation process. The invention further disclosed adistillation flask and a system for ignition-free closed flash point determination of the petroleum products. By adoption of the method for ignition-free closed flash point determination of the petroleum products, determination efficiency can be improved.

The invention discloses a chemiluminescence immunoassay kit for quantitatively measuring cat serum amyloid protein A. The kit comprises a capillary wrapping a cat serum amyloid protein A monoclonal antibody, a monoclonal antibody of the cat serum amyloid protein A labeled by alkaline phosphatase, a cat serum amyloid protein A calibration product and a enhanced chemiluminescent acting on the alkaline phosphatase. The kit can quantitatively measure the cat serum amyloid protein A, is less in sampling amount and low in cost and has the advantages of high sensitivity, specificity, accuracy, precision degree and detection efficiency and simple operation method.

The invention relates to a method for evaluating the quality of coke for a lithium battery negative electrode material, which comprises the following steps: S1, airing a green coke sample, performingcoarsely crushing, and completely performing screening by 4mm to obtain primary coke powder; s2, carrying out heat treatment on the primary coke powder in a nitrogen atmosphere at 700-800 DEG C, and then crushing the primary coke powder until the primary coke powder is completely sieved by a 0.071 mm sieve, so as to obtain secondary coke powder; and S3, carrying out vacuum drying on the secondarycoke powder at 110-140 DEG C, performing heating from 50 DEG C to 900 DEG C at a rate of 2-5 DEG C / min in an air atmosphere, carrying out thermogravimetric detection, and determining the reaction starting temperature of graphitization degree change and the reaction starting temperature of reversible capacity change. Compared with the prior art, the method has the beneficial effects that the quality grade of the green coke for the negative electrode can be rapidly distinguished, the sample consumption is small, and the analysis is rapid and sensitive; and compared with the traditional method, the method has the advantages of simplicity, convenience, time saving and the like in early-stage sample preparation.

The present invention provides a method for ultrasensitive detection of dopamine based on nucleic acid aptamers. First, ssDNA1 is combined with dopamine aptamers and ssDNA2 through the principle of complementary base pairing to form double-stranded DNA1 with two 3' protruding ends, wherein , ssDNA2 has fewer bases than ssDNA1 and is complementary to ssDNA1, and the dopamine aptamer starts from the 3' end of ssDNA2 to partially complement ssDNA1; then add the sample to be tested, hairpin DNA, fluorescent dye SYBR GreenⅠ, and exonucleic acid Enzyme III, wherein, the number of bases of the hairpin DNA is less than that of ssDNA1 and can form a double-stranded DNA2 with a recess at the 3' end of the hairpin DNA chain with the free ssDNA1 through the principle of complementary base pairing. In the present invention, the dopamine aptamer recognizes dopamine, so that the aptamer is detached from the double-stranded DNA1, thereby continuously triggering the enzyme cutting reaction, reducing the fluorescence signal, and realizing the supersensitive detection of dopamine by using the cyclic amplification technology.

The invention provides a quality control method of amygdalin in children lung heat cough and asthma granules. A thin-layer chromatography identification method has the advantages of small sample application amount, good separation effect, clear spots, moderate specific shift value and good reproducibility. Quantitative analysis is carried out on the amygdalin component in the infantile lung-heat cough and asthma granules through content determination, it is verified that the content of the amygdalin in the infantile lung-heat cough and asthma granules can be accurately and efficiently detected through content determination chromatographic conditions, and through combination of a thin-layer chromatography identification method and content, the content of amygdalin in the children lung heat cough and asthma granules can be effectively controlled, and the medication safety of the children lung heat cough and asthma granules is ensured.

The invention discloses a method for distinguishing rhizoma atractylodis in a traditional Chinese medicine compound and particularly relates to a method for thin-layer distinguishing of the rhizoma atractylodis in the traditional Chinese medicine compound. The distinguishing method comprises the following steps that a control medicinal material solution is prepared; a sample solution for test is prepared; a negative sample solution is prepared; sample application is performed on the same silica gel G thin-layer plate by using a capillary tube sample applicator; the thin-layer plate is arranged in a chromatography cylinder, development is performed by using a developing solvent, and the plate is taken out and air-dried; the thin-layer chromatographic plate is inspected, an obtained result is distinguished, main fluorescence spots of the chromatography of the sample solution for test and the control medicinal material are located in the corresponding positions and have same color, and the negative sample is free of main fluorescence spots having the same color. The distinguishing method is simple in process and good in separating effect, has strong specificity, further improves the accuracy of distinguishing the rhizoma atractylodis component in the traditional Chinese medicine compound and has huge popularization and application potential in the drug distinguishing fields relevant to the rhizoma atractylodis.

The invention relates to an online analysis method for hydrogen sulfide gas sulfur isotope in natural gas. The method includes the following steps: collecting natural gas samples; heating the oxidation furnace of the element analyzer to 1020°C; raising the temperature of the chromatographic column box to 50°C; introducing the reference gas into the stable isotope ratio mass spectrometer, and adjusting the mass-to-charge ratio to 66 The magnetic field at the center of the peak, and save the magnetic field value; connect the cylinder containing the natural gas sample to the sampling valve and put it into the fume hood, turn on the power of the fume hood; take the natural gas sample with an air-tight sampling needle; inject the natural gas sample into the In the sample port; the natural gas sample flows into the high-temperature oxidation tube with the carrier gas, and is oxidized into CO2, H2O, SO2 gas, and the mixed gas is carried by the carrier gas and flows through the dehydration tube to remove moisture; the mixed gas after removing moisture flows through the gas phase After the chromatographic column is separated, it enters the stable isotope ratio mass spectrometer through the continuous flow interface to measure the sulfur isotope composition and generate a spectrum to obtain the measurement result.