Chemiluminescence immunoassay kit for quantitatively measuring cat serum amyloid protein A

A chemiluminescence immunoassay and serum starch technology, applied in the field of immunoassay, can solve the problems of inability to quantitatively detect cat serum amyloid, large amount of serum samples, low detection efficiency, etc. The effect of wide detection range

Pending Publication Date: 2019-03-08
CHENGDU POLYTECH BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1. The carriers used in this patent are magnetic particles, microwell plates, plastic tubes or plastic beads. The use of such carriers has the disadvantages of large sample loading, large serum sample volume, and low detection efficiency;
[0008] 2. The kit provided by this patent cannot be used for the quantitative detection of feline serum amyloid A

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] This example provides a chemiluminescence immunoassay kit for quantitative determination of feline serum amyloid A. The kit includes the coated solid phase carrier of feline serum amyloid A monoclonal antibody, alkaline phosphatase (ALP)-labeled feline serum amyloid A monoclonal antibody, feline serum amyloid A calibration A chemiluminescent substrate for alkaline phosphatase. At the same time, it provides a preparation method of a reagent for quantitative detection of feline serum amyloid A, which includes: (1) pre-treatment of the solid phase carrier; (2) coating the carrier with a monoclonal antibody to feline serum amyloid A ; (3) Use alkaline phosphatase to label the monoclonal antibody of cat serum amyloid A; (4) prepare cat serum amyloid A calibrator with pure cat serum amyloid A; Serum amyloid A calibrator, alkaline phosphatase-labeled cat serum amyloid A monoclonal antibody and the chemiluminescent substrate for the enzyme; (6) assembled into a finished produc...

Embodiment 2

[0072] In this embodiment, the pre-processing steps of the capillary are specifically:

[0073] Step 1, Cleaning

[0074] Mix concentrated hydrochloric acid and ethanol at a ratio of 1:1 (volume ratio) to make a mixed solution I. Soak the capillary in the mixed solution I for 20 minutes at 22°C, take out the capillary, and rinse it with ultrapure water for 5 times , and blow dry, then soak the capillary in calcium hydroxide solution at room temperature for 20 minutes, take out the capillary, rinse with ultrapure water 5 times, and blow dry;

[0075] Step 2, Hydroxylation

[0076] Mix hydrogen peroxide and concentrated sulfuric acid at a volume ratio of 1:2) to make a mixed solution II, soak the capillary in the mixed solution II at 22°C, and after ultrasonic treatment for 20 minutes, take out the capillary and rinse it with ultrapure water for 3 times And blow dry, use calcium hydroxide solution to rinse 5 times, ethanol rinse 3 times, then rinse 5 times with ultrapure water...

Embodiment 3

[0101] In this embodiment, the pre-processing steps of the capillary are specifically:

[0102] Step 1, Cleaning

[0103] Mix concentrated hydrochloric acid and ethanol at a ratio of 1:1 (volume ratio) to make a mixed solution I, soak the capillary in the mixed solution I for 25 minutes at 28°C, take out the capillary, and rinse it with ultrapure water for 6 times , and blow dry, then soak the capillary with magnesium hydroxide solution at room temperature for 24min, take out the capillary, wash it with ultrapure water 7 times, and blow dry;

[0104] Step 2, Hydroxylation

[0105] Mix hydrogen peroxide and concentrated sulfuric acid at a ratio of 1:2.5 (volume ratio) to make a mixed solution II. Soak the capillary in the mixed solution II at 28°C. After ultrasonic treatment for 25 minutes, take out the capillary and rinse it with ultrapure water for 4 rinse with magnesium hydroxide solution for 6 times and ethanol for 4 times, then rinse with ultrapure water for 5 times and ...

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Abstract

The invention discloses a chemiluminescence immunoassay kit for quantitatively measuring cat serum amyloid protein A. The kit comprises a capillary wrapping a cat serum amyloid protein A monoclonal antibody, a monoclonal antibody of the cat serum amyloid protein A labeled by alkaline phosphatase, a cat serum amyloid protein A calibration product and a enhanced chemiluminescent acting on the alkaline phosphatase. The kit can quantitatively measure the cat serum amyloid protein A, is less in sampling amount and low in cost and has the advantages of high sensitivity, specificity, accuracy, precision degree and detection efficiency and simple operation method.

Description

technical field [0001] The invention belongs to the technical field of immune analysis, in particular to a chemiluminescent immunoassay kit for quantitatively measuring feline serum amyloid A. Background technique [0002] Feline serum amyloid A (SAA) is a precursor of amyloid deposition, which was discovered during the study of amyloidosis, and subsequently acts as an acute phase response protein, which belongs to the heterogeneity of the apolipoprotein family protein-like. Feline serum amyloid A is synthesized in the liver by activated macrophages and fibroblasts and can be elevated in the acute phase response to stimulation with interleukin-1, interleukin-6, and tumor necrosis factor to 100 -1000 times the initial concentration. During inflammation, feline serum amyloid A binds to plasma high-density lipoprotein (HDL) and regulates HDL metabolism. In addition, feline serum amyloid A degradation products can be deposited in different organs as amyloid A (AA) fibrils, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577
CPCG01N33/577
Inventor 曾妮冉鹏
Owner CHENGDU POLYTECH BIOLOGICAL TECH CO LTD
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