Quick detection method of carotenoid content in marine bivalve organism

A technology of carotene and bivalves, which is applied in the field of carotenoid content detection, can solve the problems of weighing, extraction and detection, which are time-consuming, volatile, and work-intensive, and achieve shortened detection time and process, accurate batch Measuring and improving the effect of utilization

Inactive Publication Date: 2016-05-04
SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
View PDF7 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, to date, there are few studies on large-scale, population-level rapid detection methods for CAR content in these species
Existing determination method is carried out by adopting the process and method of drying, extracting and spectrophotometric detection, but this conventional method has some disadvantages, such as: 1, time-consuming
The freeze-drying process of samples generally takes 2 to 3 days to complete and the number of samples processed each time is limited. The grinding, weighing, extraction and testing of samples are also labor-intensive and time-consuming, and the work intensity is relatively high; 2. The amount of samples used is large
For the sake of ensuring accuracy, the amount of dry samples needed each time is 0.1-0.2g, which consumes a lot of experimental samples; 3. Involving volatile drugs
The sample needs to use acetone during the extraction process. The drug is volatile at room temperature and is flammable, so it has a certain impact on the laboratory environment and safety; 4. Weak practicability
Only a small amount of samples can be measured each time, and it cannot be measured in batches, so the practicability is weak
This conventional method for determining the total carotenoids content (Totalcarotenoidscontents, TCC) is far from being applicable to the needs of large-scale, multi-batch scientific research on bivalve molluscs. Therefore, a fast, efficient and reliable method must be established. Method for Determining TCC

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Quick detection method of carotenoid content in marine bivalve organism
  • Quick detection method of carotenoid content in marine bivalve organism

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The method for rapidly detecting the content of carotenoids in marine bivalve organisms provided by this embodiment comprises the following steps:

[0038](1) Animals to be tested and sampling: 30 Chlamysnobilis Reeve to be tested came from Hainan Sanya Yiyuan Aquaculture Co., Ltd. The adductor muscle of the living body was sampled, and the closed shell was obtained after being processed with a slicer knife. Transverse tissue section of the shell muscle, the thickness is about 3mm.

[0039] (2) Tissue section color difference detection: put the tissue section in a sample groove about 2mm deep (the thickness of the tissue section is determined according to the depth of the groove, because the slice is soft, so it is required to be slightly thicker than the depth of the groove. In fact, it is only a requirement The depth of the groove is slightly smaller than the thickness of the tissue section to ensure that the thickness of the tissue section after pressing is equal to ...

Embodiment 2

[0051] The method for rapidly detecting the content of carotenoids in marine bivalve organisms provided by this embodiment comprises the following steps:

[0052] (1) Animals to be tested and sampling: the marine bivalve mollusc of Pinctadafucata to be tested comes from the cultured species in Lingshui, Hainan. The adductor muscle of the living body was sampled, and the transverse tissue section of the adductor muscle was obtained after being processed with a slicer knife, with a thickness of about 3mm;

[0053] (2) Chromatic aberration detection of tissue sections: place the tissue sections in a sample chamber with a depth of about 2mm (same as Example 1), and cover the sample with a cover glass with light-shielding treatment on the edge; press the cover glass tightly to ensure the thickness of the sample The depth is the same as that of the sample groove to ensure that the sample detection surface is smooth and the detection thickness is consistent to reduce errors; the dete...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
thicknessaaaaaaaaaa
Login to view more

Abstract

The invention discloses a quick detection method of a carotenoid content in a marine bivalve organism. The method comprises the following steps: (1) sampling and pretreatment of tissues; (2) tissue slicing and chromatic aberration detection; (3) total carotenoid content (TCC) detection; (4) establishment of a chromatic-aberration-TCC regression curve; and (5) total carotenoid content (TCC) detection of an adductor muscle of a to-be-detected marine bivalve organism. According to the method, the adductor muscle tissue of the marine bivalve organism is sliced and is subjected to chromatic aberration detection, and chromatic aberration is associated with the carotenoid content to establish a chromatic-aberration-TCC equation of linear regression. Therefore, the carotenoid content detection of the organism is detected through a corresponding chromatic aberration parameter. The method is free of extra sample consumption, has good repeatability and practicability, and is quick and efficient.

Description

technical field [0001] The invention belongs to the technical field of carotenoid content detection, in particular to a method for rapidly detecting carotenoid content in marine bivalve organisms. Background technique [0002] Carotenoids (CAR), as a polyene substance, has certain antioxidant capacity due to its polyunsaturated double bonds, and also has other biological functions. Studies have shown that the carotenoid content in shellfish has a certain correlation with the growth and life of shellfish and gonad development. For example, the study of Patinopectenyessoensis showed that the content of carotenoids in adductor muscle reaches the highest value during the gonad development period, and the content of carotenoids decreases after the reproductive period; The survival rate during the cage culture process and after spawning was higher than that of the control group, and it had significantly higher stress resistance; while the study of Chlamysnobilis Reeve found that ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N21/31G01N1/28
CPCG01N21/25G01N1/28G01N21/31
Inventor 张博喻达辉姜松刘宝锁黄桂菊李海梅郭奕惠邓正华范嗣刚
Owner SOUTH CHINA SEA FISHERIES RES INST CHINESE ACAD OF FISHERY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap