Naozhenning thin layer chromatography identification method

A technique of thin-layer chromatography and Naozhenning is applied in the field of quality control of traditional Chinese medicine extracts and preparations, which can solve the problems of unenvironmental protection and numerous steps, and achieve the effects of time saving, simple preparation method and small sampling amount.

Inactive Publication Date: 2017-09-19
SHANXI ZHENDONG ANTE BIOPHARMACEUTICAL CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing methods for identifying paeoniflorin in other Chinese medicinal materials or preparations that have been disclosed, the preparation of the test product needs to heat the organic solvent to reflux (Chinese patent CN200610201379.8, CN200810117041.3 ), or successively extract water-saturated n-butanol and n-butanol-saturated water (Chinese patent CN200810046038.7 ); the extracted solvent is eluted through a neutral alumina column (Chinese patent CN200810018308.3 ), many steps, and not environmentally friendly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1: the TLC identification method of Naozhenning Granules

[0066] According to standard WS 3 -B-3312-98 prepares sucrose-containing Naozhenning granules, and uses the identification method provided by the present invention to identify them.

[0067] Prescription: Angelica 200g, Dihuang 200g, Moutan bark 150g, Chuanxiong 150g, Dilong 150g, Salvia miltiorrhiza 375g, Poria cocos 250g, Tangerine peel 250g, Zhuru 250g, fried jujube kernel 250g, Baiziren 250g

[0068] Preparation method: the above eleven flavors, angelica, peony cortex, Chuanxiong, and tangerine peel are distilled to extract volatile oil, and the distilled aqueous solution is collected in a separate device; the dregs and the remaining seven flavors such as salvia miltiorrhiza are decocted twice, each time for 1.5 hours, and the decoction is combined. Filtrate, combine the filtrate with the above aqueous solution, concentrate to a clear paste with a relative density of 1.35-1.38 (55°C), add sucrose...

Embodiment 2

[0072] Embodiment 2: TLC identification method of Naozhenning Granules (without sucrose)

[0073] According to standard WS 3 - B-3312-98-1 prepares sucrose-free Naozhenning Granules, which are identified by the identification method provided by the present invention.

[0074] Prescription: Angelica 200g, Dihuang 200g, Moutan bark 150g, Chuanxiong 150g, Dilong 150g, Salvia miltiorrhiza 375g, Poria cocos 250g, Tangerine peel 250g, Zhuru 250g, fried jujube kernel 250g, Baiziren 250g

[0075]The above eleven flavors, angelica, Moutan cortex, Chuanxiong, and tangerine peel are distilled to extract volatile oils, and the distilled aqueous solution is collected in a separate device; the dregs and the remaining seven flavors such as Salvia miltiorrhiza are decocted twice, each time for 1.5 hours, and the decoctions are combined and filtered. Combine the filtrate with the above aqueous solution, concentrate to a clear paste with a relative density of 1.35-1.38 (55°C), add dextrin, gra...

Embodiment 3

[0078] Embodiment 3: TLC identification method of Naozhenning Capsules

[0079] Prescription: Angelica 220g, Rehmannia glutinosa 210g, Moutan bark 160g, Chuanxiong 140g, Dilong 130g, Salvia miltiorrhiza 330g, Poria cocos 260g, tangerine peel 260g, Zhuru 260g, fried jujube kernel 160g, Baiziren 160g

[0080] Preparation method: the above eleven flavors, angelica, peony bark, chuanxiong, tangerine peel are distilled to extract volatile oil, and then add other medicinal materials for water extraction. After water extraction, use 80% ethanol to precipitate twice, concentrate into a thick paste, add starch and mix Homogenize, granulate, dry and granulate, add the obtained volatile oil, and stir evenly to prepare 200 Naozhenning capsules, each containing 0.4g.

[0081] Identification: (1) Take 0.77g of the contents of Naozhenning Capsules, grind it finely, add 10mL of methanol, ultrasonicate for 10 minutes, filter, evaporate the filtrate to dryness, add 0.5mL of methanol to dissolve...

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Abstract

The invention discloses a TLC identification method for Nazhenning, which belongs to the technical field of quality control of traditional Chinese medicine extracts and preparations. The invention provides the identification of tangerine peel in Naozhenning. The preparation method of the test product is simple, fast, accurate and environmentally friendly. The crude drug contained in it can be quantified, the separation degree is high, the sampling volume is small, the sampling volume is controllable, the sensitivity is high, and the interference is less. The invention provides the identification of Cortex Moutan in Naozhenning, using methanol ultrasonic extraction, water-saturated n-butanol extraction, neutral alumina mixing sample, can obtain the test sample, the sample amount is small, save time, and reduce environmental pollution Less, faster and easier to operate. The method provided by the invention has strong specificity, good reproducibility and durability, and can be used for the identification of the corresponding medicinal flavors in the extracts and preparations of Zhenzhengning.

Description

technical field [0001] The invention relates to a TLC identification method for Nazhenning, which belongs to the technical field of quality control of traditional Chinese medicine extracts and preparations. Background technique [0002] Naozhenning is an extract and Chinese patent medicine for the prevention and treatment of concussion, post-traumatic brain syndrome, post-neurosurgery recovery period and can be made into granules according to the prescription disclosed in the patent No. 931007410 , capsules, tablets, soft capsules and other suitable dosage forms. In order to control the quality of Naozhenning preparation, we conducted a TLC identification study on it. [0003] Naozhenning granules are granules made according to a patented prescription. At present, Youtang Granules are included in the "Ministry of Health of the People's Republic of China Drug Standards for Traditional Chinese Medicine Prescriptions (Volume 17)" on page 230 (standard number: WS 3 -B-3312-98...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90G01N1/28
CPCG01N30/90G01N1/28
Inventor 李昆刘海霞朱平秦正国范玛莉秦文杰杨晓宁赵丽敏
Owner SHANXI ZHENDONG ANTE BIOPHARMACEUTICAL CO LTD
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